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Information on EC 3.1.1.73 - feruloyl esterase and Organism(s) Aspergillus tubingensis and UniProt Accession O42815
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3.1.1.73 feruloyl esteraseIUBMB Comments
Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates. p-Nitrophenol acetate and methyl ferulate are poorer substrates. All microbial ferulate esterases are secreted into the culture medium. They are sometimes called hemicellulase accessory enzymes, since they help xylanases and pectinases to break down plant cell wall hemicellulose.
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Word Map
- 3.1.1.73
- aspergillus
- esterases
- wheat
- niger
- bran
- xylans
- arabinoxylans
-
hydroxycinnamic
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p-coumaric
- thermocellum
- hemicellulose
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destarched
- caffeate
- fermentum
- degradation
- synthesis
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talaromyces
-
xylanolytic
- xylanases
-
diferulic
-
psycinfo
- sinapinate
- nutrition
- spelt
- arabinofuranosidase
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ester-linked
-
ensile
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lignocellulolytic
- awamori
- acetylxylan
- neocallimastix
-
fibrolytic
- food industry
- agriculture
- paper production
- biotechnology
- pharmacology
- biofuel production
- industry
- medicine
The taxonomic range for the selected organisms is: Aspergillus tubingensis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
feruloyl esterase, ferulic acid esterase, cinii, anfaea, feruloyl esterase a, fae-iii, cinnae, type a feruloyl esterase, xylanase z, fae-ii, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
cinnAE
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cinnamoyl ester hydrolase
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-
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Cinnamoyl esterase
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-
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EstA
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-
-
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FAE-I
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-
-
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FAE-II
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-
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FAE-III
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-
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FAEA
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-
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ferulic acid esterase
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feruloyl/p-coumaroyl esterase
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Feruloylesterase
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hemicellulase acessory enzymes
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hydroxycinnamoyl esterase
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phenolic acid esterase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
carboxylic ester hydrolysis
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-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
4-hydroxy-3-methoxycinnamoyl-sugar hydrolase
Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates. p-Nitrophenol acetate and methyl ferulate are poorer substrates. All microbial ferulate esterases are secreted into the culture medium. They are sometimes called hemicellulase accessory enzymes, since they help xylanases and pectinases to break down plant cell wall hemicellulose.
CAS REGISTRY NUMBER
COMMENTARY
134712-49-5
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224306-54-1
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224306-55-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
feruloyl-polysaccharide + H2O
ferulate + polysaccharide
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-
-
-
?
additional information
?
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FaeA shows preference to substrates with methoxy substituents
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-
?
additional information
?
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crude enzyme extracts, containing feruloyl esterase and polygalacturonase, removes the mucilage of coffee cherries within 3 h, which is substantially more efficient than traditional fermentation, the viscosity of coffee mucilage is reduced to 80% by a 3-h treatment with the crude enzyme extract at 50°C. Total chlorogenic acid in the green beans decreases to 67.3%, while a decline of only 14.3% is observed in the traditionally fermented group. On the other hand, chlorogenic acid lactones in the roasted beans are reduced to 63.9%, and a 37.2% decline in the chlorogenic acid content is detected
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
feruloyl-polysaccharide + H2O
ferulate + polysaccharide
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-
-
-
?
additional information
?
-
-
crude enzyme extracts, containing feruloyl esterase and polygalacturonase, removes the mucilage of coffee cherries within 3 h, which is substantially more efficient than traditional fermentation, the viscosity of coffee mucilage is reduced to 80% by a 3-h treatment with the crude enzyme extract at 50°C. Total chlorogenic acid in the green beans decreases to 67.3%, while a decline of only 14.3% is observed in the traditionally fermented group. On the other hand, chlorogenic acid lactones in the roasted beans are reduced to 63.9%, and a 37.2% decline in the chlorogenic acid content is detected
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-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
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solid-state fermentation with pectin and destarched wheatbran as carbon sources
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). FAEA_ASPTU
280
0
30451
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
PCR cloning and sequence analysis, gene faeA encoding ferulic acid esterase cloned, the gene product FAEA overexpressed in wild-type Aspergillus tubingensis and protease-deficient Aspergillus niger mutant, gene product is identical with Aspergillus niger FAE-III
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
food industry
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potential for the application of Aspergillus tubingensis enzyme extract in coffee processing. Crude enzyme extracts, containing feruloyl esterase and polygalacturonase, removes the mucilage of coffee cherries within 3 h, which is substantially more efficient than traditional fermentation, the viscosity of coffee mucilage is reduced to 80% by a 3-h treatment with the crudeenzyme extract at 50°C. Total chlorogenic acid in the green beans decreases to 67.3%, while a decline of only 14.3% is observed in the traditionally fermented group. On the other hand, chlorogenic acid lactones in the roasted beans are reduced to 63.9%, and a 37.2% decline in the chlorogenic acid content is detected
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
De Vries, R.P.; Michelsen, B.; Poulsen, C.H.; Kroon, P.A.; van den Heuvel, R.H.; Faulds, C.B.; Williamson, G.; van den Hombergh, J.P.; Visser, J.
The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides
Appl. Environ. Microbiol.
63
4638-4644
1997
Aspergillus niger, Aspergillus niger N402, Aspergillus tubingensis, Butyrivibrio fibrisolvens
Blum, D.L.; Kataeva, I.A.; Li, X.L.; Ljungdahl, L.G.
Feruloyl esterase activity of the Clostridium thermocellum cellulosome can be attributed to previously unknown domains of XynY and XynZ
J. Bacteriol.
182
1346-1351
2000
Aspergillus niger, Aspergillus tubingensis, Butyrivibrio fibrisolvens, Acetivibrio thermocellus, Neocallimastix sp., no activity in Escherichia coli, Orpinomyces sp., Pseudomonas fluorescens, Ruminococcus sp., Acetivibrio thermocellus JW20
Blum, D.L.; Schubot, F.D.; Ljungdahl, L.G.; Rose, J.P.; Wang, B.C.
Crystallization and preliminary x-ray analysis of the Clostridium thermocellum cellulosome xylanase Z feruloyl esterase domain
Acta Crystallogr. Sect. D
56
1027-1029
2000
Aspergillus niger, Aspergillus tubingensis, Butyrivibrio fibrisolvens, Acetivibrio thermocellus, Orpinomyces sp., Piromyces sp. 'equi', Pseudomonas fluorescens
-
Topakas, E.; Vafiadi, C.; Christakopoulos, P.
Microbial production, characterization and applications of feruloyl esterases
Process Biochem.
42
497-509
2007
Aspergillus oryzae, Aureobasidium pullulans, Aspergillus niger (O42807), Aspergillus niger (Q8WZI8), Aspergillus tubingensis (O42815), Acetivibrio thermocellus (P10478), Butyrivibrio fibrisolvens (P70884), Butyrivibrio fibrisolvens (P94315), Aspergillus nidulans (Q5BCF8), Aspergillus awamori (Q9P979)
-
Koseki, T.; Fushinobu, S.; Ardiansyah, S.; Shirakawa, H.; Komai, M.
Occurrence, properties, and applications of feruloyl esterases
Appl. Microbiol. Biotechnol.
84
803-810
2009
Aspergillus luchuensis, Aspergillus niger, Aspergillus niger (O42807), Aspergillus niger (Q8WZI8), Aspergillus oryzae, Aureobasidium pullulans, Thermothelomyces heterothallicus, Acetivibrio thermocellus, Fusarium oxysporum, Neocallimastix sp., Talaromyces stipitatus, Cellvibrio japonicus, Salmonella enterica subsp. enterica serovar Typhimurium (G2QND5), Aspergillus tubingensis (O42815), Penicillium chrysogenum (Q3V6C9), Aspergillus nidulans (Q5BCF8), Piromyces sp. E2 (Q870B0), Talaromyces funiculosus (Q9HE18), Neurospora crassa (Q9HGR3), Orpinomyces sp. PC-2 (Q9P8Y0), Aspergillus awamori (Q9P979), Piromyces sp. 'equi' (Q9Y871)
Tai, E.; Hsieh, P.; Sheu, S.
Effect of polygalacturonase and feruloyl esterase from Aspergillus tubingensis on demucilage and quality of coffee beans
Process Biochem.
49
1274-1280
2014
Aspergillus tubingensis
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