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Information on EC 3.1.1.7 - acetylcholinesterase and Organism(s) Lucilia cuprina and UniProt Accession P91954
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3.1.1.7 acetylcholinesteraseIUBMB Comments
Acts on a variety of acetic esters; also catalyses transacetylations.
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- 3.1.1.7
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cholinergic
- alzheimer
- nerve
- butyrylcholinesterase
-
organophosphate
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pesticide
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neurotoxicity
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organophosphorus
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insecticide
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muscarinic
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innervation
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poison
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histochemistry
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neurochemical
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monoamine
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intoxication
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antidote
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anticholinergic
- environmental protection
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The taxonomic range for the selected organisms is: Lucilia cuprina
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ache, acetylcholinesterase, acetylcholine esterase, acetyl cholinesterase, hache, eeache, ache1, huache, tcache, membrane-bound acetylcholinesterase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AcCholE
-
-
-
-
acetyl beta-methylcholinesterase
-
-
-
-
acetylcholine esterase
-
-
-
-
acetylcholine hydrolase
-
-
-
-
acetylthiocholinesterase
-
-
-
-
AChE
-
-
-
-
choline esterase I
-
-
-
-
cholinesterase
-
-
-
-
esterase, acetyl choline
-
-
-
-
true cholinesterase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
transacetylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
acetylcholine acetylhydrolase
Acts on a variety of acetic esters; also catalyses transacetylations.
CAS REGISTRY NUMBER
COMMENTARY
9000-81-1
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-methoxyacetylthiocholine + H2O
thiocholine + 2-methoxyacetate
recombinant enzyme shows about 65% activity compared to acetylthiocholine
-
-
?
3-methylbutyrylthiocholine + H2O
thiocholine + 3-methylbutyrate
recombinant enzyme shows less than 1% activity compared to acetylthiocholine
-
-
?
acetyl-homo-thiocholine + H2O
?
recombinant enzyme shows less than 10% activity compared to acetylthiocholine
-
-
?
benzoylthiocholine + H2O
thiocholine + benzoate
recombinant enzyme shows about 1% activity compared to acetylthiocholine
-
-
?
butyrylthiocholine + H2O
thiocholine + butyrate
native enzyme shows about 50% activity and recombinant enzyme shows about 45% activity compared to acetylthiocholine
-
-
?
cyclopentylcarbonylthiocholine + H2O
thiocholine + cyclopentanecarboxylate
recombinant enzyme shows less than 1% activity compared to acetylthiocholine
-
-
?
cyclopropylcarbonylthiocholine + H2O
thiocholine + cyclopropanecarboxylate
recombinant enzyme shows about 5% activity compared to acetylthiocholine
-
-
?
hexanoylthiocholine + H2O
thiocholine + hexanoate
recombinant enzyme shows less than 1% activity compared to acetylthiocholine
-
-
?
isobutyrylthiocholine + H2O
thiocholine + isobutyrate
recombinant enzyme shows about 15% activity compared to acetylthiocholine
-
-
?
pentanoylthiocholine + H2O
thiocholine + pentanoate
recombinant enzyme shows about 5% activity compared to acetylthiocholine
-
-
?
phenylacetylthiocholine + H2O
thiocholine + phenylacetate
recombinant enzyme shows about 3% activity compared to acetylthiocholine
-
-
?
pivaloylthiocholine + H2O
thiocholine + pivalate
recombinant enzyme shows less than 1% activity compared to acetylthiocholine
-
-
?
propionylthiocholine + H2O
thiocholine + propionate
native and recombinant enzyme show about 70% activity compared to acetylthiocholine
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0355
acetylthiocholine
native enzyme from head extract, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.0381
acetylthiocholine
recombinant enzyme, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.0568
butyrylthiocholine
native enzyme from head extract, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.072
butyrylthiocholine
recombinant enzyme, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.0139
propionylthiocholine
recombinant enzyme, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.0213
propionylthiocholine
native enzyme from head extract, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.001557
9-amino-1,2,3,4-tetrahydroacridin-1-ol
Lucilia cuprina
at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.03
aldicarb-sulfoxide
Lucilia cuprina
IC50 above 0.03 mM, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.03
butocarboxim
Lucilia cuprina
IC50 above 0.03 mM, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.03
butoxycarboxim
Lucilia cuprina
IC50 above 0.03 mM, at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.000014
heptylene-bis-tacrine
Lucilia cuprina
at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
0.000004
tetraethylpyrophosphate
Lucilia cuprina
at 22°C in 10 mM Na2HPO4/NaH2PO4, pH 7.4
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
the enzyme is cell-surface-exposed and possesses a glycosyl-phosphatidylinositol membrane anchor
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). P91954_LUCCU
708
0
78453
TrEMBL
Mitochondrion (Reliability: 2)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ilg, T.; Cramer, J.; Lutz, J.; Noack, S.; Schmitt, H.; Williams, H.; Newton, T.
The characterization of Lucilia cuprina acetylcholinesterase as a drug target, and the identification of novel inhibitors by high throughput screening
Insect Biochem. Mol. Biol.
41
470-483
2011
Homo sapiens, Lucilia cuprina (P91954), Lucilia cuprina
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