Information on EC 3.1.1.5 - lysophospholipase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.1.1.5
-
RECOMMENDED NAME
GeneOntology No.
lysophospholipase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
degradation of sugar alcohols
-
-
Glycerophospholipid metabolism
-
-
phospholipid remodeling (phosphatidylethanolamine, yeast)
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-
SYSTEMATIC NAME
IUBMB Comments
2-lysophosphatidylcholine acylhydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-85-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Col-0
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
adult Beijing white laying hen
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
isolate Pb18
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-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
lysoPL2 knockout mutants show enhanced sensitivity to Zn2+ and H2O2 in comparison to wild type
metabolism
-
the enzyme exerts important functions in the homeostasis of phospholipids or detoxification of lysophospholipids
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2,3-trioleoyl-sn-glycerol + H2O
?
show the reaction diagram
1,2-didecanoylphosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
?
1,2-didodecanoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
?
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
1,2-dioleoylglycerol + H2O
?
show the reaction diagram
-
-
-
-
?
1,2-dioleoylphosphatidylcholine + H2O
monooleoyl-lysophosphatidylcholine + oleic acid
show the reaction diagram
-
129% of activity with 1,2-dipalmitoylphosphatidylcholine
-
?
1,2-dioleoylphosphatidylethanolamine + H2O
monooleoyl-lysophosphatidylethanolamine + oleic acid
show the reaction diagram
-
-
33.5% of activity with 1,2-dipalmitoylphosphatidylcholine
?
1,2-dioleoylphosphatidylserine + H2O
monooleoyl-lysophosphatidylserine + oleic acid
show the reaction diagram
-
-
22.5% of activity with 1,2-dipalmitoylphosphatidylcholine
?
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-sn-glycero-3-phosphocholine + palmitate
show the reaction diagram
-
-
-
?
1,2-dipalmitoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
1,2-dipalmitoylphosphatidylcholine + H2O
?
show the reaction diagram
1,2-palmitylphosphatidylcholine + H2O
1-palmityl-lysophosphatidylcholine + palmitic acid
show the reaction diagram
-
low activity after activating of ExoU with an unknown eukaryotic cell factor
-
?
1-acyl-2-lysophosphatidycholine + H2O
fatty acid + glycerophosphocholine
show the reaction diagram
-
-
-
?
1-acyl-glycerophosphoglycerol + H2O
fatty acid + glcyerophosphoglycerol
show the reaction diagram
-
-
-
-
?
1-acyl-lysophosphatidylcholine + 1-alkyllysophosphatidylcholine
1-alkyl-2-acyl-phosphatidylcholine + glycerylphosphocholine
show the reaction diagram
-
-
-
?
1-acyl-sn-glycero-3-phosphate + H2O
fatty acid + glyceryl phosphate
show the reaction diagram
-
-
-
-
?
1-acyl-sn-glycero-3-phospho-myo-inositol
fatty acid + glycero-3-phospho-myo-inositol
show the reaction diagram
1-acyl-sn-glycero-3-phosphocholine + H2O
fatty acid + glycero-3-phosphocholine
show the reaction diagram
1-acyl-sn-glycero-3-phosphoserine + H2O
fatty acid + glycerophosphoserine
show the reaction diagram
1-acylglycerophosphoethanolamine + H2O
fatty acid + glycerylphosphoethanolamine
show the reaction diagram
1-arachidonoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-docosahexaenoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-dodecanoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
?
1-hexanoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-hexanoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + hexanoate
show the reaction diagram
-
-
-
-
?
1-hexyl-glycerophosphocholine + H2O
hexanoate + glycerophosphocholine
show the reaction diagram
-
-
-
-
?
1-lauroyl lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-lauroyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-lauroyl-sn-glycero-3-phosphocholine + H2O
lauric acid + sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-lauryl-glycerophosphocholine + H2O
laurate + glycerophosphocholine
show the reaction diagram
-
-
-
-
?
1-linoleoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-linoleoyl-sn-glycero-3-phosphocholine + H2O
linoleic acid + glycerophosphorylcholine
show the reaction diagram
-
-
-
-
?
1-monopalmitoylglycerol + H2O
glycerol + palmitic acid
show the reaction diagram
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-
lipase activity of LPLA
?
1-monopalmitoyllysophosphatidylglycerol + H2O
glycerophosphoglycerol + palmitic acid
show the reaction diagram
-
-
?
1-myristoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-myristoyl-sn-glycero-3-phosphocholine + H2O
myristic acid + sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-octanoyl-sn-glycero-3-phosphocholine + H2O
octanoic acid + sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
-
?
1-oleoyl lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-oleoyl lysophosphatidylcholine + H2O
glycerophosphocholine + oleate
show the reaction diagram
-
30% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1
-
-
?
1-oleoyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-oleoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-oleoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + oleate
show the reaction diagram
-
-
-
-
?
1-oleoyl-2-lysophosphatidylcholine + H2O
glycerophosphorylcholine + oleic acid
show the reaction diagram
-
30% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0, 667% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 7.0
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-
?
1-oleoyl-glycerophosphocholine + H2O
oleate + glycerophosphocholine
show the reaction diagram
-
-
-
-
?
1-oleoyl-lysophosphatidylcholine + H2O
glycerophosphorylcholine + oleic acid
show the reaction diagram
-
194% of activity with 1-palmitoyl-lysophosphatidylcholine
-
?
1-oleoyl-sn-glycero-3-phosphocholine + H2O
oleic acid + glycerophosphorylcholine
show the reaction diagram
1-oleoyl-sn-glycero-3-phosphoethanolamine + H2O
oleic acid + glycero-3-phosphoethanolamine
show the reaction diagram
1-palmitoyl lysophosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
1-palmitoyl lysophosphatidylcholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
1-palmitoyl lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-acetyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
1-palmitoyl-2-lyso-sn-phosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
?
show the reaction diagram
1-palmitoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
1-palmitoyl-2-lysophosphatidylcholine + H2O
palmitate + glycerophosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-2-oleoylphosphatidylcholine + H2O
lysophosphatidylcholine + ?
show the reaction diagram
-
36% of activity with 1,2-dipalmitoylphosphatidylcholine
-
?
1-palmitoyl-glycerophosphocholine + H2O
palmitate + glycerophosphocholine
show the reaction diagram
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
1-palmitoyl-lysophosphatidylethanolamine + H2O
glycerophosphatidylethanolamine + palmitic acid
show the reaction diagram
1-palmitoyl-lysophosphatidylethanolamine + H2O
glycerophosphoethanolamine + palmitate
show the reaction diagram
-
20% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
1-palmitoyl-sn-glycero-3-phosphoethanolamine + H2O
palmitic acid + glycero-3-phosphoethanolamine
show the reaction diagram
1-palmitoyl-sn-glycero-3-phosphoserine + H2O
glycerophosphoserine + palmitic acid
show the reaction diagram
1-palmitoyl-sn-glycerol + H2O
palmitic acid + glycerol
show the reaction diagram
-
-
-
-
?
1-stearoyl-2-arachidonoyl-sn-glycerol
?
show the reaction diagram
-
-
-
-
?
1-stearoyl-glycerophosphocholine + H2O
stearate + glycerophosphocholine
show the reaction diagram
-
-
-
-
?
1-stearoyl-sn-glycero-3-phosphocholine + H2O
stearic acid + glycerophosphorylcholine
show the reaction diagram
1-stearoyl-sn-glycero-3-phosphoethanolamine + H2O
stearic acid + glycero-3-phosphoethanolamine
show the reaction diagram
2,3-dioleoylglycerol + H2O
?
show the reaction diagram
-
-
-
-
?
2,3-dipalmitoyl-sn-glycero-1-phosphocholine + H2O
monopalmitoyl-sn-glycero-1-phosphocholine + palmitic acid
show the reaction diagram
2-acylglycerophosphocholine + H2O
fatty acid + glycerylphosphocholine
show the reaction diagram
2-acylglycerophosphoethanolamine + H2O
fatty acid + glycerylphosphoethanolamine
show the reaction diagram
-
-
-
-
?
2-acylglycerophosphoglycerol + H2O
fatty acid + glcyerophosphoglycerol
show the reaction diagram
-
-
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a fatty acid
show the reaction diagram
-
-
-
?
2-palmitoyl-sn-glycero-3-phosphocholine + H2O
palmitic acid + glycero-3-phosphocholine
show the reaction diagram
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
show the reaction diagram
4-nitrophenyl caprylate + H2O
4-nitrophenol + caprylate
show the reaction diagram
-
-
-
?
4-nitrophenyl decanoate + H2O
4-nitrophenol + decanoate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl laurate + H2O
4-nitrophenol + laurate
show the reaction diagram
relative activity: 7.56%
-
-
?
4-nitrophenyl myristate + H2O
4-nitrophenol + myristate
show the reaction diagram
4-nitrophenyl palmitate + H2O
4-nitrophenol + palmitate
show the reaction diagram
4-nitrophenyl stearate + H2O
4-nitrophenol + stearate
show the reaction diagram
C10:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C12:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C14:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C18:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C18:1-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C18:2-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
C8:0-glycero-3-phosphocholine + H2O
?
show the reaction diagram
-
rank order for lysophospholipase activity C12:0-GPC higher than C14:0-GPC, C10:0-GPC higher than C18:0-GPC, C18:1-GPC, and C18:2-GPC higher than C8:0-GPC
-
-
?
dioleoylphosphatidylcholine + H2O
?
show the reaction diagram
-
2% of the activity with dipalmitoylphosphatidylcholine
-
-
?
dioleoylphosphatidylethanolamine + H2O
?
show the reaction diagram
-
11% of the activity with dipalmitoylphosphatidylcholine
-
-
?
dipalmitoylphosphatidylcholine + H2O
?
show the reaction diagram
-
-
-
-
?
ghrelin + H2O
des-acyl ghrelin + n-octanoic acid
show the reaction diagram
-
-
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine
show the reaction diagram
-
lysophospholipase A activity
-
-
?
lysophosphatidylcholine + H2O
glycerophosphorylcholine + unesterified fatty acid
show the reaction diagram
lysophosphatidylcholine + lysophosphatidylcholine
glycerophosphocholine + phosphatidylcholine
show the reaction diagram
lysophosphatidylethanolamine + H2O
?
show the reaction diagram
-
-
-
-
?
lysophosphatidylethanolamine + H2O
fatty acid + glycerylphosphoethanolamine
show the reaction diagram
lysophosphatidylglycerol + H2O
fatty acid + glycerol
show the reaction diagram
-
-
-
-
?
lysophosphatidylinositol + H2O
glycerophosphatidylinositol + a carboxylate
show the reaction diagram
-
78% of activity with 1-palmitoyl-lysophosphatidylcholine
-
?
monooleoylglycerol + H2O
oleic acid + glycerol
show the reaction diagram
-
-
-
-
?
monopalmitoylglycerol + H2O
palmitic acid + glycerol
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
p-nitrophenyl butyrate
p-nitrophenol + butyrate
show the reaction diagram
substrate activity assay
-
-
?
p-nitrophenyl palmitate
p-nitrophenol + palmitate
show the reaction diagram
substrate activity assay
-
-
?
palmitoyl-Gialpha1 + H2O
palmitic acid + Gialpha1
show the reaction diagram
-
thioesterase activity
-
?
palmitoyl-glycero-3-phosphocholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
-
?
palmitoyl-peptide + H2O
palmitate + peptide
show the reaction diagram
residue 3 to 14 of RGS4 protein with palmitoyl group attached at Cys12 of RGS4 protein
-
-
?
palmitoyl-SCoA + H2O
HS-CoA + palmitic acid
show the reaction diagram
-
-
-
?
palmitoyllysophosphatidylglycerol + H2O
glycerophosphoglycerol + palmitic acid
show the reaction diagram
-
-
-
-
?
phosphatidic acid + H2O
?
show the reaction diagram
phosphatidylcholine + H2O
lysophosphatidylcholine + a carboxylate
show the reaction diagram
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
show the reaction diagram
phosphatidylcholine + H2O
lysophosphatidylcholine + glycerophosphorylcholine + a fatty acid
show the reaction diagram
-
-
-
?
phosphatidylethanolamine + H2O
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a carboxylate
show the reaction diagram
phosphatidylethanolamine + H2O
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a fatty acid
show the reaction diagram
-
-
-
?
phosphatidylglycerol + H2O
lysophosphatidylglycerol + a carboxylate
show the reaction diagram
-
94.2% of activity with 1,2-dipalmitoylphosphatidylcholine
-
?
phosphatidylinositol + H2O
lysophophatidylinositol + a carboxylate
show the reaction diagram
phosphatidylinositol + H2O
lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
show the reaction diagram
phosphatidylserine + H2O
lysophosphatidylserine + glycerophosphorylserine + a carboxylate
show the reaction diagram
phosphatidylserine + H2O
lysophosphatidylserine + glycerophosphorylserine + a fatty acid
show the reaction diagram
-
-
-
?
platelet activating factor + H2O
platelet activating factor + a carboxylate
show the reaction diagram
-
8.9% of activity with 1,2-dipalmitoylphosphatidylcholine
-
?
sphingomyelin + H2O
?
show the reaction diagram
-
3% of the activity with dipalmitoylphosphatidylcholine
-
-
?
triacetin + H2O
?
show the reaction diagram
-
weak activity
-
-
?
tributyrin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-acyl-2-lysophosphatidycholine + H2O
fatty acid + glycerophosphocholine
show the reaction diagram
Q9U8F2
-
-
-
?
1-monopalmitoyllysophosphatidylglycerol + H2O
glycerophosphoglycerol + palmitic acid
show the reaction diagram
Q8GNM7
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + H2O
glycerophosphocholine + palmitic acid
show the reaction diagram
-
-
-
?
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
glycerophosphocholine + palmitate
show the reaction diagram
Q8GNM7
-
-
?
2-lysophosphatidylcholine + H2O
glycerophosphocholine + a carboxylate
show the reaction diagram
lysophosphatidylcholine + H2O
glycerophosphorylcholine
show the reaction diagram
-
lysophospholipase A activity
-
-
?
phosphatidylcholine + H2O
lysophosphatidylcholine + a carboxylate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity, to 8% at pH 5.0 and 4% at pH 7.0
Co2+
pH 8.0, activates
CoCl2
-
10 mM, stimulates
CuSO4
-
10 mM, stimulates
Hg2+
significantly inhibits enzyme activity
HgCl2
-
10 mM, stimulates
K3Fe(CN)6
-
activates
MgCl2
-
10 mM, stimulates
Mn2+
pH 8.0, activates
MnCl2
-
10 mM, stimulates
NiSO4
-
10 mM, stimulates
ZnCl2
-
10 mM, stimulates
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,12-bis(tributylammonium)dodecane
-
-
1,12-bis(trihexylammonium)dodecane
-
-
1,12-bis(triisopentylammonium)dodecane
-
-
1,12-bis(tripentylammonium)dodecane
-
-
1,12-bis(tripropylammonium)dodecane
-
-
1-palmitoyl lysophosphatidylcholine
competitive inhibitor for acylprotein thioesterase activity
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphate
2-Hydroxy-5-nitrobenzyl bromide
-
-
3(cis,cis-7,10)hexadecadienyl-4-hydroxy-2-butenolide
-
-
AACOCF3
-
a trifluoromethyl ketone analog of arachidonic acid, uncompetitive
albumin
-
-
-
alexidine
-
specific inhibitor, 66% inhibition at 0.00025 mM
arachidonic acid
-
-
arachidonyl trifluoromethyl ketone
-
0.025 mM, approx. 50% inhibition
Ba2+
-
-
bis(p-nitrophenyl)phosphate
-
0.01 mM, 8% inhibition of lysophospholipase I and 61% inhibition of lysophospholipase II
bromoenol lactone
-
0.025 mM, approx. 30% inhibition
CaCl2
-
10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity
calcium dipicolinate
-
71% residual activity at 1 mM
carnitine
-
10 mM, 38% inhibition of phospholipase B activity; 10 mM, 9% inhibition at pH 7.0, LPL1
Cetyltrimethylammonium bromide
-
-
chlorpromazine
-
at low concentrations significantly stimulates phospholipase B activity of native enzyme, strong stimulation at high concentrations
chlorpyrifos pentyl oxon
-
0.000076 mM, 50% inhibition
Co2+
-
-
D-alanine
-
94% residual activity at 1 mM
deoxycholate
dichlorvos
-
0.000028 mM, 50% inhibition
diisopropylfluorophosphate
dipalmitoyl phosphatidylcholine
-
0.5 mM, 84% inhibition at pH 5.0, 50% inhibition at pH 7.0, LPL 1
dipalmitoylphosphatidylcholine
-
0.5 mM, 89% inhibition of lysophospholipase at pH 5.0, 82% inhibition of lysophospholipase at pH 7.0
diphenyl fluorophosphinate
-
2 mM, 52% inhibition
dipicolinate
-
82% residual activity at 1 mM
Dithionitrobenzoate
-
-
dithiothreitol
DNA containing thymine glycol
EGTA
-
10 mM, 15% inhibition at pH 7.0, LPL1
EPTC sulfoxide
-
0.2 mM, 51% inhibition
ethyl octylphosphonofluoridate
FeCl3
-
10 mM, 39% inhibition at pH 5.0, 38% inhibition at pH 7.0, LPL1; 10 mM, 80% inhibiotion of phospholipase B activity, 17% inhibition of lysophospholipase activity at pH 5.0, 29% in inhibition of lysophospholipase activity at pH 7.0
glutathione
-
membrane-bound enzyme, no inhibition of the soluble enzyme
Insulin
-
insulin reduces NRE mRNA levels in a dose-dependent manner in 3T3-L1 adipocytes
-
iodoacetamide
iodoacetate
-
-
isopropyl dodecylphosphonofluoridate
L-alanine
-
93% residual activity at 1 mM
L-linoleoylcarnitine
-
-
L-palmitoylcarnitine
lysophosphatidic acid
methyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14- tetraenylphosphonofluoridate
-
-
Mn2+
-
67% residual activity at 1 mM
N-bromosuccinimide
-
-
n-dodecyl-benzodioxaphosphorine 2-oxide
n-octadecyl-benzodioxaphosphorine 2-oxide
-
0.000067 mM, 50% inhibition
p-hydroxymercuribenzoate
-
-
palmitic acid
-
-
palmitoyl carnitine
-
0.5 mM, 90% inhibition at pH 5.0, LPL1; 1 mM, 64% inhibition of phospholipase B activity, 65% inhibition of lysophospholipase activity at pH 5.0
palmitoyl-peptide
competitive inhibitor for lysophospholipase activity
palmitoylcarnitine
phenyl N-benzylcarbamate
-
0.2 mM, 47% inhibition
Phenylglyoxal
-
-
phenylmethylsulfonyl fluoride
phosphatidic acid
PMSF
-
little effect
S-n-octyl-benzodioxaphosphorine 2-oxide
-
0.000005 mM, 50% inhibition
sphingomyelin
-
0.5 MM, 21% inhibition at pH 5.0, 29% inhibition at pH 7.0, LPL1; 0.5 mM, 35% inhibition of phospholipase B activity, 45% inhibition of lysophospholipase at pH 5.0, 28% inhibition of lysophospholipase at pH 7.0
sulfite
-
enzymatic activity: 1.6%
tellurate
-
enzymatic activity: 19.8%
tellurite
-
enzymatic activity: 0%
-
tri-o-cresyl phosphate
TOCP
tricyclodecan-9-yl-xanthogenate potassium
-
-
-
trifluoromethyl ketone
-
0.025 mM, approx. 40% inhibition
Triton X-100
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
minimizes loss of activity in presence of sulfhydryl reagent
carnitine
-
10 mM, activation to 121% of control at pH 5.0, LPL1
Chloramphenicol
-
stimulates phospholipase B activity
dithiothreitol
-
10 mM, activation to 122% of control at pH 5.0, LPL1
ethanol
ethanol increases the enzyme activity to 136%
glycerol
-
50% increase of lysophospholipase activity in the presence of 30% glycerol
Isopropanol
isopropanol increases the enzyme activity to 133%
lysophosphatidylcholine
-
0.5 mM, activation of phospholipase B activity to 132% of control
methanol
methanol increases the enzyme activity to 111%
palmitoyl carnitine
-
0.5 mM, stimulates to 157% of control at pH 7.0, LPL1; 1 mM, activation of lysophospholipase activity at pH 7.0 to 239% of control
palmitoylcarnitine
-
stimulates hydrolase activity
pulmonary surfactant
-
enzyme activity significantly increases (28%) in the presence of 0.1 mg/ml pulmonary surfactant
-
Sodium deoxycholate
Triton X-100
additional information
-
pilD-dependent lysophospholipase
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
1,2-didecanoyl-sn-glycero-3-phosphocholine
-
-
1.1
1,2-didecanoylphosphatidylcholine
-
2.9
1,2-dioctanoyl-sn-glycero-3-phosphocholine
-
-
0.82
1,2-dipalmitoyl-sn-glycero-3-phosphocholine
-
-
0.19
1,2-dipalmitoylphosphatidylcholine
-
37C, pH 4.0
0.04498
1-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-2-lysophosphatidylcholine
-
Vmax: 103.5 micromol/h/mg protein
1.6
1-acyl-2-oleoyl-phosphatidylcholine
-
-
0.0676
1-arachidonoyl lysophosphatidylcholine
0.04498
1-docosahexaenoyl lysophosphatidylcholine
-
Vmax: 103.5 micromol/h/mg protein
0.0992
1-hexanoyl lysophosphatidylcholine
7.22
1-hexyl-glycerophosphocholine
-
at pH 8.0 and 37C
-
0.1203
1-lauroyl lysophosphatidylcholine
1.78
1-lauryl-glycerophosphocholine
-
at pH 8.0 and 37C
-
0.08892
1-linoleoyl lysophosphatidylcholine
0.127
1-myristoyl lysophosphatidylcholine
0.306
1-oleoyl lysophosphatidylcholine
0.0273 - 0.19
1-palmitoyl lysophosphatidylcholine
0.19
1-palmitoyl-2-lysophosphatidylcholine
-
Vmax: 90.9 micromol/h/mg protein
2.04
1-palmitoyl-glycerophosphocholine
-
at pH 8.0 and 37C
0.092
1-palmitoyl-lysophosphatidylcholine
-
37C, pH 4.0
0.076
1-palmitoyl-sn-glycero-3-phosphatidylcholine
-
-
0.0606 - 0.17
1-palmitoyl-sn-glycero-3-phosphocholine
0.75
2-oleoyl-lysophosphatidylcholine
-
-
0.6071
4-nitrophenyl butyrate
in 50 mM HEPES (pH 8.0), at 85C
0.0065
ghrelin
-
-
-
0.132
lysolecithin
-
-
0.022 - 0.26
lysophosphatidylcholine
0.00349
palmitoyl-peptide
-
0.22
palmitoyllysophosphatidylglycerol
-
-
0.18 - 0.63
phosphatidylcholine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1016
1-hexyl-glycerophosphocholine
Pseudomonas aeruginosa
-
at pH 8.0 and 37C
-
1975
1-lauryl-glycerophosphocholine
Pseudomonas aeruginosa
-
at pH 8.0 and 37C
-
921.1
1-palmitoyl-glycerophosphocholine
Pseudomonas aeruginosa
-
at pH 8.0 and 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
110
1-hexyl-glycerophosphocholine
Pseudomonas aeruginosa
-
at pH 8.0 and 37C
197702
1100
1-lauryl-glycerophosphocholine
Pseudomonas aeruginosa
-
at pH 8.0 and 37C
197703
450
1-palmitoyl-glycerophosphocholine
Pseudomonas aeruginosa
-
at pH 8.0 and 37C
16477
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0222
1-palmitoyl lysophosphatidylcholine
for acylprotein thioesterase activity
0.0323
palmitoyl-peptide
for lysophospholipase activity
0.16
tellurite
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
1,12-bis(tributylammonium)dodecane
Cryptococcus neoformans
-
-
0.0075
1,12-bis(trihexylammonium)dodecane
0.0035
1,12-bis(tripentylammonium)dodecane
Cryptococcus neoformans
-
-
0.007
1,12-bis(tripropylammonium)dodecane
Cryptococcus neoformans
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.404
-
purified enzyme, at pH 8.0 and 37C
0.5
-
activity with palmitoyllysophosphatidylglycerol
1.34
-
-
1.62
substrate 1-palmitoyl lysophosphatidylcholine
1.66
-
lysophospholipase II
2.3
-
activity with ghrelin
2.31
-
lysophospholipase I
2.448
-
-
10.5
-
hydrolysis of 1,2-dipalmitoylphosphatidylcholine
27.3
substrate palmitoyl-peptide
40
-
phospholipase B activity
77.68
-
hydrolase activity
117
-
fatty acid release
193
-
lysophospholipase activity
320.9
-
transacylase activity
459
-
phosphatidylcholine formation
559
-
hydrolysis of 1-palmitoyl-lysophosphatidylcholine
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2
and a second optimum at pH 7.5
3
two pH-optima: pH 3.0 and pH 7.5
4.5 - 5
-
phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1
5
-
lysophospholipase activity and transacylase activity, short incubation time (30 s), LPL1
5.5 - 8
-
lysophospholipase activity, long incubation time (5 min), LPL1
6 - 8
-
deacylation of 2-acyllysophosphatidylcholine, lysophospholipase I
6.5
-
and a second optimum at pH 8.0, membrane-bound enzyme
6.5 - 7
-
hydrolysis of 1-palmitoyl-glycero-3-phosphorylcholine
7.3
-
activity assay
7.5 - 9
-
-
9.5
-
and a second optimum at pH 7.0, soluble enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.2 - 3
-
pH 2.2: about 70% of maximal activity, pH 3.0: about 65% of maximal activity
2.5 - 6
-
approx. 50% of maximal phospholipase activity at pH 3.0 and pH 5.0, respectively
2.5 - 7
-
50-60% of maximal lysophospholipase activity at pH 3.0 and pH 5.5, respectively
4 - 7
-
pH 4.0: about 45% of maximal activity, pH 7.0: about 85% of maximal activity, lysophosphatidylcholine transacylase activity, pH 4.0: about 50% of maximal activity, pH 7.0: about 80% of maximal activity, lysophosphatidyl hydrolase activity
4 - 8
-
pH 4.0: about 65% of maximal activity, pH 8.0: about 40% of maximal activity, production of phosphatidylcholine
4 - 7
-
pH 4.0: about 55% of maximal activity, pH 7.0: about 60% of maximal activity
4 - 11
-
41% of maximal activity at pH 5.0, 79% of maximal activity at pH 6, Semimembranosus proprius muscle, 33% of maximal acitivity at pH 5, 75% of maximal activity at pH 6, Longissimus lumborum muscle
4.2 - 6
-
pH 4.2: about 75% of maximal lysophospholipase activity, pH 6.0: about 75% of maximal lysophospholipase activity, short incubation time (30 s), LPL1
5 - 9.3
-
pH 5.0: about 30% of maximal activity, pH 9.3: about 35% of maximal activity
7
-
no phospholipase B activity, no lysophospholipase activity and no transacylase activity of PLB1 is detected at short incubation time (30 s), at incubation time 5-10 min lysophospholipase activity and transacylase activity is detected (about 5% of the activity at pH 5.0), no phospholipase B activity is detected at incubation time 5-10 min
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
lysophospholipase activity
25
activity assay
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
-
25C: about 70% of maximal activity, 50C: about 90% of maximal activity
30 - 80
conformational changes are negligible over the temperature range of 30-80C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
-
2D-electrophoresis
3.9
-
isoelectric focusing
5.1
-
isoelectric focusing, 63000 Da species
6.05
determined by isoelectric focusing, SDS-PAGE, extract of adult Schistosoma japonicum worm
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest expression
Manually annotated by BRENDA team
primary cultures of mouse neural cells. PLB activity is present in a variety of cultured mouse neural cell types but that acute loss of this activity is not cytotoxic
Manually annotated by BRENDA team
-
macrophage-like
Manually annotated by BRENDA team
isoform lysophospholipase I only
Manually annotated by BRENDA team
-
in vitro storage for 48 h does not affect activity
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
spore coat
Manually annotated by BRENDA team
-
hLysoPLA I expression is induced by phorbol 12-myristate 13-acetate
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
two-third of the enzyme is particulate, one-third is cytoplasmic
Manually annotated by BRENDA team
-
enzyme is also located in mitochondria even in the absence of prenylation
Manually annotated by BRENDA team
-
two-third of the enzyme is particulate, one-third is cytoplasmic
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21000
-
lysophospholipase I, gel filtration
25000
-
gel filtration
29000
determined by SDS-PAGE
30000
-
lysophospholipase I, gel filtration
32040
calculated
35500
calculated from cDNA
42000
determined by SDS-PAGE and Western Blot analysis
49000
-
gel filtration
52000
-
a 52000 Da species and a 63000 Da species is detected by SDS-PAGE.The 52000 Da species is a truncated version of the 63000 Da protein
57000
-
lysophospholipase II, SDS-PAGE
58000
-
gel filtration
61000
-
SDS-PAGE, Flag-tagged fusion protein
65000
-
SDS-PAGE
90000
-
gel filtration
116000
-
gel filtration
130000
determined by gel filtration
140000 - 232000
-
native PAGE
160000 - 180000
-
gel filtration
220000
-
isoenzyme LPL1, gel filtratiin
275000
-
isoenzyme PLB1, gel filtratiin
280000 - 420000
-
gel filtration
300000
-
gel filtration
330000
-
gel filtration
additional information
-
-
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decamer
-
10 * 66000, isoenzyme LPL1, SDS-PAGE
dimer
-
2 * or 3 x * 100000-150000, SDS-PAGE
homodimer
prediction, based on the determination of the hydrodynamic radius by dynamic light scattering measurements
monomer
trimer
-
2 * or 3 x * 100000-150000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
side-chain modification