Information on EC 3.1.1.48 - fusarinine-C ornithinesterase

for references in articles please use BRENDA:EC3.1.1.48
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.1.48
-
RECOMMENDED NAME
GeneOntology No.
fusarinine-C ornithinesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N5-acyl-L-ornithine ester + H2O = N5-acyl-L-ornithine + an alcohol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
hydrolysis of ester
SYSTEMATIC NAME
IUBMB Comments
N5-acyl-L-ornithine-ester hydrolase
Hydrolyses the three ornithine ester bonds in fusarinine C. Also acts on N5-dinitrophenyl-L-ornithine methyl ester.
CAS REGISTRY NUMBER
COMMENTARY hide
60202-10-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Fusarium roseum
ATCC 12822
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
fusarinine B + H2O
fusarinine A + fusarinine
show the reaction diagram
Fusarium roseum
-
-
-
?
fusarinine C + H2O
?
show the reaction diagram
fusarinine C + H2O
fusarinine A + fusarinine
show the reaction diagram
Fusarium roseum
-
-
-
?
N,N',N''-triacetylfusarinine C + H2O
?
show the reaction diagram
Fusarium roseum
-
-
-
-
?
N5-dinitrophenyl-L-ornithine methyl ester + H2O
N5-dinitrophenyl-L-ornithine + methanol
show the reaction diagram
triacetylfusarinine C + H2O
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
fusarinine C + H2O
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
HgCl2
Fusarium roseum
-
-
N-Bromoacetamide
Fusarium roseum
-
-
N-ethylmaleimide
Fusarium roseum
-
-
N5-Dinitrophenyl-L-ornithine methyl ester
Fusarium roseum
-
-
p-chloromercuribenzoate
Fusarium roseum
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
N5-Dinitrophenyl-L-ornithine methyl ester
Fusarium roseum
-
-
0.4
triacetylfusarinine C
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.1
Fusarium roseum
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
Fusarium roseum
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
Fusarium roseum
-
about 50% of maximal activity at pH 6.5 and pH 9.0, no activity at pH 10.0
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 37
-
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32700
-
calculation from sequence of cDNA
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
Fusarium roseum
-
significant loss of activity after 10 min
40
Fusarium roseum
-
85% loss of activity after 20 min
50
Fusarium roseum
-
complete loss of activity after 20 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Talon metal resin affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE