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Information on EC 3.1.1.4 - phospholipase A2 and Organism(s) Bothrops insularis and UniProt Accession Q8QG87

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.4 phospholipase A2
IUBMB Comments
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
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This record set is specific for:
Bothrops insularis
UNIPROT: Q8QG87
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The taxonomic range for the selected organisms is: Bothrops insularis
The enzyme appears in selected viruses and cellular organisms
Synonyms
phospholipase a2, cpla2, spla2, spla(2), prdx6, cytosolic phospholipase a2, crotoxin, pla2s, ipla2, spla2-iia, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14 kDa phospholipase A2
-
-
-
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Agkistrotoxin
-
-
-
-
amdI1
-
-
-
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Ammodytin I2
-
-
-
-
APLA
-
-
-
-
APP-D-49
-
-
-
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ASPLA1
-
-
-
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ASPLA10
-
-
-
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ASPLA11
-
-
-
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ASPLA12
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-
-
-
ASPLA13
-
-
-
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ASPLA14
-
-
-
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ASPLA15
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-
-
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ASPLA16
-
-
-
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ASPLA17
-
-
-
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ASPLA2
-
-
-
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ASPLA3
-
-
-
-
ASPLA4
-
-
-
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ASPLA5
-
-
-
-
ASPLA6
-
-
-
-
ASPLA7
-
-
-
-
ASPLA8
-
-
-
-
ASPLA9
-
-
-
-
ATX
-
-
-
-
Basic protein I/II
-
-
-
-
BJ-PLA2
-
-
-
-
BJUPLA2
-
-
-
-
BPI/BPII
-
-
-
-
CaI-PLA2
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-
-
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Caudoxin
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-
-
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cPm09
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-
-
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Enhancing factor
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-
-
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GIIC sPLA2
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-
-
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GIID sPLA2
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-
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GIIE sPLA2
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-
-
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GIIF sPLA2
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-
-
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GIII sPLA2
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-
-
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Group IB phospholipase A2
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-
-
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Group IIA phospholipase A2
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-
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Group V phospholipase A2
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-
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Group VI phospholipase A2
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-
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GVI PLA2
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-
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GX sPLA2
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-
-
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GXII sPLA2
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-
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GXIII sPLA2
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-
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iPLA2
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-
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lecithinase A
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-
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MP-III 4R
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-
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Muscarinic inhibitor
-
-
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Myotoxin
-
-
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NAJPLA-2A
-
-
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NAJPLA-2B
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NAJPLA-2C
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-
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Nigexine
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-
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Non-pancreatic secretory phospholipase A2
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-
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Notechis 11'2
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-
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Notexin
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-
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NPLA
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-
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NPS-PLA2
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-
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OHV A-PLA2
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-
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OHV-APLA2
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pgPLA 1a/pgPLA 2a
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phosphatidase
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phosphatide 2-acylhydrolase
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phosphatidolipase
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Phosphatidylcholine 2-acylhydrolase
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Phosphatidylcholine 2-acylhydrolase GIIC
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Phosphatidylcholine 2-acylhydrolase GIID
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Phosphatidylcholine 2-acylhydrolase GIIE
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Phosphatidylcholine 2-acylhydrolase GIIF
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Phosphatidylcholine 2-acylhydrolase GIII
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Phosphatidylcholine 2-acylhydrolase GX
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Phosphatidylcholine 2-acylhydrolase GXII
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Phosphatidylcholine 2-acylhydrolase GXIII
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phospholipase A
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Phospholipase A2 inhibitor
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pkP5
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PLA2-10
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PLA2-VI
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PLA2-VII
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PLA2IID
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platelet activating factor acetyl hydrolase
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Pt-PLA1
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Pt-PLA2
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Secretory-type PLA, stroma-associated homolog
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sPLA(2)-IID
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-
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sPLA(2)-IIE
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-
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sPLA(2)-IIF
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-
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TMV-K49
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-
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Toxin VI
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Toxin VI:5
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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-
-
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SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine 2-acylhydrolase
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-84-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitro-3-(octanoyloxy)benzoic acid + H2O
?
show the reaction diagram
-
-
-
?
4-nitro-3-octanoyloxy-benzoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.49
isozyme BinTx-II, sequence calculation
5.05
isozyme BinTx-I, sequence calculation
8.6
-
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isozyme BinTx-I; two isozymes BinTx-I and BinTx-II
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
isozymes BinTx-I and BinTx-II
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
isozymes BinTx-I and BinTx-II
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PA2A_BOTIN
138
0
15759
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
13788
x * 13975, isozyme BinTx-I, sequence calculation, x * 13788, isozyme BinTx-II, sequence calculation
13975
x * 13975, isozyme BinTx-I, sequence calculation, x * 13788, isozyme BinTx-II, sequence calculation
13984
-
x * 13984, mass spectrometry, x * 14000, SDS-PAGE
14000
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x * 13984, mass spectrometry, x * 14000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 13975, isozyme BinTx-I, sequence calculation, x * 13788, isozyme BinTx-II, sequence calculation
?
-
x * 13984, mass spectrometry, x * 14000, SDS-PAGE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native PLA2 from venom in several chromatographic steps, including gel filtration and anion exchange chromatography, to over 95% puritiy
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
isozymes BinTx-I and BinTx-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis, sequence comparisons, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cogo, J.C.; Lilla, S.; Souza, G.H.; Hyslop, S.; de Nucci, G.
Purification, sequencing and structural analysis of two acidic phospholipases A2 from the venom of Bothrops insularis (jararaca ilhoa)
Biochimie
88
1947-1959
2006
Bothrops insularis (Q8QG87), Bothrops insularis
Manually annotated by BRENDA team
Machado Braga, M.D.; Costa Martins, A.M.; Alves, C.D.; Beserra de Menezes, D.; Martins, R.D.; Ferreira Barbosa, P.S.; Oliveira, I.M.; Toyama, M.H.; Toyama, D.O.; dos Santos Diz Filho, E.B.; Ramos Fagundes, F.H.; Fonteles, M.C.; Azul Monteiro, H.S.
Purification and renal effects of phospholipase A2 isolated from Bothrops insularis venom
Toxicon
51
181-190
2008
Bothrops insularis
Manually annotated by BRENDA team