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Information on EC 3.1.1.4 - phospholipase A2 and Organism(s) Gloydius halys and UniProt Accession P14418

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.4 phospholipase A2
IUBMB Comments
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
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This record set is specific for:
Gloydius halys
UNIPROT: P14418
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The taxonomic range for the selected organisms is: Gloydius halys
The enzyme appears in selected viruses and cellular organisms
Synonyms
phospholipase a2, cpla2, spla2, spla(2), prdx6, cytosolic phospholipase a2, crotoxin, pla2s, ipla2, spla2-iia, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14 kDa phospholipase A2
-
-
-
-
Agkistrotoxin
-
-
-
-
AgkTx-II
-
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amdI1
-
-
-
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Ammodytin I2
-
-
-
-
APLA
-
-
-
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APP-D-49
-
-
-
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Asp49 basic myotoxic PLA2
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ASPLA1
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-
-
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ASPLA10
-
-
-
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ASPLA11
-
-
-
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ASPLA12
-
-
-
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ASPLA13
-
-
-
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ASPLA14
-
-
-
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ASPLA15
-
-
-
-
ASPLA16
-
-
-
-
ASPLA17
-
-
-
-
ASPLA2
-
-
-
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ASPLA3
-
-
-
-
ASPLA4
-
-
-
-
ASPLA5
-
-
-
-
ASPLA6
-
-
-
-
ASPLA7
-
-
-
-
ASPLA8
-
-
-
-
ASPLA9
-
-
-
-
ATX
-
-
-
-
basic Asp49 PLA2
-
-
Basic protein I/II
-
-
-
-
BJ-PLA2
-
-
-
-
BJUPLA2
-
-
-
-
BPI/BPII
-
-
-
-
CaI-PLA2
-
-
-
-
Caudoxin
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-
-
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cPm09
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-
-
-
Enhancing factor
-
-
-
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GIIC sPLA2
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-
-
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GIID sPLA2
-
-
-
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GIIE sPLA2
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-
-
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GIIF sPLA2
-
-
-
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GIII sPLA2
-
-
-
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Group IB phospholipase A2
-
-
-
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Group IIA phospholipase A2
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-
-
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Group V phospholipase A2
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-
-
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Group VI phospholipase A2
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-
-
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GVI PLA2
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-
-
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GX sPLA2
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-
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GXII sPLA2
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-
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GXIII sPLA2
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-
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iPLA2
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-
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lecithinase A
-
-
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MP-III 4R
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-
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Muscarinic inhibitor
-
-
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Myotoxin
-
-
-
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NAJPLA-2A
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-
-
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NAJPLA-2B
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-
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NAJPLA-2C
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-
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Nigexine
-
-
-
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Non-pancreatic secretory phospholipase A2
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-
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Notechis 11'2
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-
-
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Notexin
-
-
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NPLA
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-
-
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NPS-PLA2
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-
-
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OHV A-PLA2
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-
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OHV-APLA2
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-
-
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pgPLA 1a/pgPLA 2a
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-
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phosphatidase
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phosphatide 2-acylhydrolase
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phosphatidolipase
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Phosphatidylcholine 2-acylhydrolase
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Phosphatidylcholine 2-acylhydrolase GIIC
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Phosphatidylcholine 2-acylhydrolase GIID
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Phosphatidylcholine 2-acylhydrolase GIIE
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Phosphatidylcholine 2-acylhydrolase GIIF
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Phosphatidylcholine 2-acylhydrolase GIII
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Phosphatidylcholine 2-acylhydrolase GX
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Phosphatidylcholine 2-acylhydrolase GXII
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Phosphatidylcholine 2-acylhydrolase GXIII
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phospholipase A
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Phospholipase A2 inhibitor
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pkP5
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PLA2-10
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-
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PLA2-VI
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PLA2-VII
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PLA2IID
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platelet activating factor acetyl hydrolase
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-
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Pt-PLA1
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-
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Pt-PLA2
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Secretory-type PLA, stroma-associated homolog
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sPLA(2)-IID
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-
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sPLA(2)-IIE
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-
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sPLA(2)-IIF
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-
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TMV-K49
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-
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Toxin VI
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Toxin VI:5
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine 2-acylhydrolase
Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-84-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
egg yolk phospholipid + H2O
?
show the reaction diagram
-
-
-
-
?
phosphatidylcholine + H2O
1-acylglycerophosphocholine + fatty acid
show the reaction diagram
phosphatidylcholine + H2O
lysophosphatidylcholine + a fatty acid
show the reaction diagram
-
-
-
-
?
phospholipids + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphatidylcholine + H2O
lysophosphatidylcholine + a fatty acid
show the reaction diagram
-
-
-
-
?
phospholipids + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-bromophenacyl bromide
-
inhibits activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1500
-
de-/renatured acidic PLA2, PC for substrate
1600
-
de-/renatured basic-acidic PLA2, PA for substrate
1750
-
de-/renatured basic PLA2, PA for substrate
500
-
de-/renatured basic PLA2, PC for substrate
62000
-
pH 8.0, 30°C
650
-
de-/renatured basic-acidic PLA2, PC for substrate
750
-
de-/renatured acidic PLA2, PA for substrate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PA2A_GLOHA
124
0
13974
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14000
-
x * 14000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 14000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2.0 A resolution, R-factor of 0.157
acidic and basic PLA2
-
to 2.3 A resolution, space group P222, with unit-cell parameters a = 44.27, b = 68.39, c = 81.54 A
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from crude venom
-
native enzyme from venom by gel filtration and C8 reverse phase HPLC
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
basic-acidic hybrid PLA2 cDNA, cloned into the expression vector pBLMVL2 for later transformation in Escherichia coli
-
expressed in Escherichia coli
-
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
basic-acidic hybrid PLA2
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
in mouse aorti rings, enzyme can induce a further contractile response on the 60 mM K+-induced contraction, with an EC50 of 369 nM
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Liu, X.; Pan, H.; Yang, G.; Wu, X.; Zhou, Y.
Cloning, expression and biochemical characterization of a basic-acidic hybrid phospholipase A2-II from Agkistrodon halys Pallas
Biochim. Biophys. Acta
1431
157-165
1999
Gloydius halys
Manually annotated by BRENDA team
Wang, X.Q.; Yang, J.; Gui, L.L.; Lin, Z.J.; Chen, Y.C.; Zhou, Y.C.
Crystal structure of an acidic phospholipase A2 from the venom of Agkistrodon halys pallas at 2.0 A resolution
J. Mol. Biol.
255
669-676
1996
Gloydius halys (P14418), Gloydius halys
Manually annotated by BRENDA team
Zhong, X.; Liu, J.; Wu, X.; Zhou, Y.
Expression, purification and biochemical characterization of a recombinant phospholipase A2, with anticoagulant activity from Agkistrodon halys Pallas
J. Nat. Toxins
10
17-25
2001
Gloydius halys
Manually annotated by BRENDA team
Perumal Samy, R.; Gopalakrishnakone, P.; Ho, B.; Chow, V.T.
Purification, characterization and bactericidal activities of basic phospholipase A2 from the venom of Agkistrodon halys (Chinese pallas)
Biochimie
90
1372-1388
2008
Gloydius halys
Manually annotated by BRENDA team
Zou, Z.; Zeng, F.; Zhang, L.; Niu, L.; Teng, M.; Li, X.
Purification, crystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A2 with vasoconstrictor activity from Agkistrodon halys pallas venom
Acta Crystallogr. Sect. F
68
1329-1332
2012
Gloydius halys
Manually annotated by BRENDA team