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Information on EC 3.1.1.29 - aminoacyl-tRNA hydrolase and Organism(s) Pyrococcus horikoshii and UniProt Accession O74017

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.29 aminoacyl-tRNA hydrolase
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This record set is specific for:
Pyrococcus horikoshii
UNIPROT: O74017 not found.
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Word Map
The taxonomic range for the selected organisms is: Pyrococcus horikoshii
The enzyme appears in selected viruses and cellular organisms
Synonyms
peptidyl-trna hydrolase, ankzf1, ptrhd1, spovc, mspth, bacterial peptidyl-trna hydrolase, peptidyl-trna hydrolase 2, yhr189w, abpth, cdc48 adaptor, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
peptidyl-tRNA hydrolase
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peptidyl-tRNA hydrolase 2
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PH1539
locus name
alanyl-tRNA editing protein AlaX-M
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AlaX-M trans-editing enzyme
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aminoacyl-transfer ribonucleate hydrolase
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-
-
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hydrolase, aminoacyl-transfer ribonucleate
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-
-
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N-substituted aminoacyl transfer RNA hydrolase
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-
-
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peptidyl-tRNA hydrolase
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-
-
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PTH
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
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-
-
-
PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
aminoacyl-tRNA aminoacylhydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9054-98-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-substituted aminoacyl-tRNA + H2O
N-substituted amino acid + tRNA
show the reaction diagram
-
-
-
?
Gly-tRNAAla + H2O
Gly + tRNAAla
show the reaction diagram
-
-
-
?
Ser-tRNAAla + H2O
Ser + tRNAAla
show the reaction diagram
-
-
-
?
additional information
?
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no activity with N-formyl-methionyl-tRNA
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-substituted aminoacyl-tRNA + H2O
N-substituted amino acid + tRNA
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
a zinc ion is coordinated by the conserved zinc-binding cluster in the C-domain, which is expected to be the enzymatic active site
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
peptidyl-tRNA hydrolase is an essential enzymewhich acts as one of the rescue factors of the stalled ribosomes. This enzyme is required for rapid clearing of the peptidyl-tRNAs, the accumulation of which in the cell leads to cell death
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25300
dynamic light-scattering
25000
gel filtration
25300
1 * 25300, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
x-ray crystallography
monomer
1 * 25300, calculated from sequence
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method at 20°C, crystal structure is determined at 2.7 A resolution
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C703A
mutation abolishes editing activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Super Q Toyopearl 650M column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shimizu, K.; Kuroishi, C.; Sugahara, M.; Kunishima, N.
Structure of peptidyl-tRNA hydrolase 2 from Pyrococcus horikoshii OT3: insight into the functional role of its dimeric state
Acta Crystallogr. Sect. D
64
444-453
2008
Pyrococcus horikoshii (O74017), Pyrococcus horikoshii OT-3 (O74017)
Manually annotated by BRENDA team
Fukunaga, R.; Yokoyama, S.
Structure of the AlaX-M trans-editing enzyme from Pyrococcus horikoshii
Acta Crystallogr. Sect. D
63
390-400
2007
Pyrococcus horikoshii (O57848), Pyrococcus horikoshii
Manually annotated by BRENDA team
Sharma, S.; Kaushik, S.; Sinha, M.; Kushwaha, G.S.; Singh, A.; Sikarwar, J.; Chaudhary, A.; Gupta, A.; Kaur, P.; Singh, T.P.
Structural and functional insights into peptidyl-tRNA hydrolase
Biochim. Biophys. Acta
1844
1279-1288
2014
Acinetobacter baumannii, Escherichia coli, Francisella tularensis, Methanocaldococcus jannaschii (Q60363), Mycobacterium tuberculosis, Mycolicibacterium smegmatis, Pseudomonas aeruginosa, Pyrococcus horikoshii (O74017), Pyrococcus horikoshii OT-3 (O74017), Saccharolobus solfataricus, Saccharolobus solfataricus P2
Manually annotated by BRENDA team