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delta-dodecanolactone + H2O
5-hydroxydodecanoate
-
-
-
?
delta-nonalactone + H2O
5-hydroxynonanoate
-
-
-
?
delta-undecanolactone + H2O
5-hydroxyundecanoate
-
-
-
?
dihydrocoumarin + H2O
3-(2-hydroxyphenyl)-propionic acid
-
-
-
?
epsilon-caprolactone + H2O
?
-
-
-
?
gamma-butyrolactone + H2O
4-hydroxybutanoate
-
-
-
?
gamma-dodecanolactone + H2O
4-hydroxydodecanoate
-
-
-
?
gamma-heptanolactone + H2O
4-hydroxyheptanoate
-
-
-
?
gamma-nonalactone + H2O
4-hydroxynonanoate
-
-
-
?
gamma-undecanolactone + H2O
4-hydroxyundecanoate
-
-
-
?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
N-(3-oxododecanoyl)-L-homoserine
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
?
N-(3-oxohexanoyl)homoserine lactone + H2O
N-(3-oxohexanoyl)homoserine
-
-
-
?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
-
-
-
?
N-(3-oxooctanoyl)homoserine lactone + H2O
N-(3-oxooctanoyl)homoserine
-
-
-
?
N-butanoylhomoserine lactone + H2O
N-butanoylhomoserine
-
-
-
?
N-dodecanoylhomoserine lactone + H2O
N-dodecanoylhomoserine
-
-
-
?
N-hexanoylhomoserine lactone + H2O
N-hexanoylhomoserine
-
-
-
?
N-octanoylhomoserine lactone + H2O
N-octanoylhomoserine
-
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
-
-
?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
-
-
-
?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
the best, possibly natural, substrate of wild-type enzyme
-
-
?
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1.678
delta-dodecanolactone
pH 8.3, 25°C, wild-type enzyme
0.359
delta-nonalactone
pH 8.3, 25°C, wild-type enzyme
0.057 - 0.219
delta-undecanolactone
1.376
dihydrocoumarin
pH 8.3, 25°C, wild-type enzyme
0.234
epsilon-caprolactone
pH 8.3, 25°C, wild-type enzyme
1.22
gamma-dodecanolactone
pH 8.3, 25°C, wild-type enzyme
0.166
gamma-heptanolactone
pH 8.3, 25°C, wild-type enzyme
2.943
gamma-nonalactone
pH 8.3, 25°C, wild-type enzyme
0.013 - 2.099
gamma-undecanolactone
0.143 - 1.605
N-(3-oxodecanoyl)-L-homoserine lactone
0.0178 - 0.456
N-(3-oxododecanoyl)-L-homoserine lactone
0.558
N-(3-oxohexanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.592
N-(3-oxohexanoyl)homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.123
N-(3-oxooctanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.256
N-(3-oxooctanoyl)homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.345
N-dodecanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.459
N-hexanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.145
N-octanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.057
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263V
0.094
delta-undecanolactone
pH 8.3, 25°C, wild-type enzyme
0.13
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263T
0.135
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263F
0.161
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263M
0.219
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263L
0.013
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263T
0.334
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263M
0.361
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263I
0.372
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263L
0.373
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263F
1.76
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263V
2.099
gamma-undecanolactone
pH 8.3, 25°C, wild-type enzyme
0.143
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.288
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263F
1
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263T
1.346
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263V
1.605
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263I
0.0178
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263I
0.0247
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263V
0.137
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263T
0.146
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263F
0.456
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.7
paraoxon
pH 8.3, 25°C, mutant enzyme W263F
0.93
paraoxon
pH 8.3, 25°C, mutant enzyme W263M
24.2
paraoxon
pH 8.3, 25°C, wild-type enzyme
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15.32
delta-nonalactone
pH 8.3, 25°C, wild-type enzyme
7.38 - 93.3
delta-undecanolactone
7.32
dihydrocoumarin
pH 8.3, 25°C, wild-type enzyme
4.45
epsilon-caprolactone
pH 8.3, 25°C, wild-type enzyme
2.72
gamma-dodecanolactone
pH 8.3, 25°C, wild-type enzyme
2.92
gamma-heptanolactone
pH 8.3, 25°C, wild-type enzyme
5.54
gamma-nonalactone
pH 8.3, 25°C, wild-type enzyme
1.94 - 5.64
gamma-undecanolactone
0.107 - 4.52
N-(3-oxodecanoyl)-L-homoserine lactone
0.41 - 6.44
N-(3-oxododecanoyl)-L-homoserine lactone
0.083
N-(3-oxohexanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.041
N-(3-oxohexanoyl)homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.54
N-(3-oxooctanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.42
N-(3-oxooctanoyl)homoserine lactone
pH 8.3, 25°C, wild-type enzyme
1.7
N-dodecanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.36
N-hexanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
1
N-octanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
7.38
delta-undecanolactone
pH 8.3, 25°C, wild-type enzyme
12.65
delta-undecanolactone
pH 8.3, 25°C, wild-type enzyme
44.8
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263V
56.8
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263L
58
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263I
66.5
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263F
71.2
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263M
93.3
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263T
1.94
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263I
3.92
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263L
4.25
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263M
4.55
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263T
4.63
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263F
4.95
gamma-undecanolactone
pH 8.3, 25°C, wild-type enzyme
5.64
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263V
0.107
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263T
0.19
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263V
0.6
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263I
3.96
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263F
4.52
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.41
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263F
1.01
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
1.8
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263I
3
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263V
6.44
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263T
6.82
paraoxon
pH 8.3, 25°C, mutant enzyme W263M
8.47
paraoxon
pH 8.3, 25°C, mutant enzyme W263F
12.6
paraoxon
pH 8.3, 25°C, wild-type enzyme
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7.54
delta-dodecanolactone
pH 8.3, 25°C, wild-type enzyme
42.7
delta-nonalactone
pH 8.3, 25°C, wild-type enzyme
78.6 - 792
delta-undecanolactone
5.32
dihydrocoumarin
pH 8.3, 25°C, wild-type enzyme
19
epsilon-caprolactone
pH 8.3, 25°C, wild-type enzyme
1.2
gamma-butyrolactone
pH 8.3, 25°C, wild-type enzyme
2.23
gamma-dodecanolactone
pH 8.3, 25°C, wild-type enzyme
17.6
gamma-heptanolactone
pH 8.3, 25°C, wild-type enzyme
1.88
gamma-nonalactone
pH 8.3, 25°C, wild-type enzyme
2.36 - 349
gamma-undecanolactone
0.106 - 31.6
N-(3-oxodecanoyl)-L-homoserine lactone
2.2 - 121
N-(3-oxododecanoyl)-L-homoserine lactone
0.15
N-(3-oxohexanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.069
N-(3-oxohexanoyl)homoserine lactone
pH 8.3, 25°C, wild-type enzyme
4.39
N-(3-oxooctanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
1.63
N-(3-oxooctanoyl)homoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.012
N-butanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
4.9
N-dodecanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
0.78
N-hexanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
6.9
N-octanoylhomoserine lactone
pH 8.3, 25°C, wild-type enzyme
78.6
delta-undecanolactone
pH 8.3, 25°C, wild-type enzyme
259
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263L
442
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263M
492
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263F
717
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263T
792
delta-undecanolactone
pH 8.3, 25°C, mutant enzyme W263V
2.36
gamma-undecanolactone
pH 8.3, 25°C, wild-type enzyme
3.2
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263V
5.37
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263I
10.5
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263L
12.4
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263F
12.7
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263M
349
gamma-undecanolactone
pH 8.3, 25°C, mutant enzyme W263T
0.106
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263T
0.141
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263V
0.374
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263I
13.8
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263F
31.6
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
2.2
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
2.22
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, wild-type enzyme
2.81
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263F
47
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263T
101
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263I
121
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.3, 25°C, mutant enzyme W263V
0.519
paraoxon
pH 8.3, 25°C, wild-type enzyme
0.883
paraoxon
pH 8.3, 25°C, mutant enzyme W263V
1.06
paraoxon
pH 8.3, 25°C, mutant enzyme W263T
1.21
paraoxon
pH 8.3, 25°C, mutant enzyme W263I
2.37
paraoxon
pH 8.3, 25°C, mutant enzyme W263L
7.33
paraoxon
pH 8.3, 25°C, mutant enzyme W263M
12.1
paraoxon
pH 8.3, 25°C, mutant enzyme W263F
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