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EC Tree
IUBMB Comments Acts on many phenolic esters. The reactions of EC 3.1.8.1 aryldialkylphosphatase, were previously attributed to this enzyme. It is likely that the three forms of human paraoxonase are lactonases rather than aromatic esterases [7,8]. The natural substrates of the paraoxonases are lactones [7,8], with (+/-)-5-hydroxy-6E,8Z,11Z,4Z-eicostetraenoic-acid 1,5-lactone being the best substrate .
The taxonomic range for the selected organisms is: Mus musculus The enzyme appears in selected viruses and cellular organisms
Synonyms
paraoxonase, arylesterase, paraoxonase 1, paraoxonase-1, paraoxonase1, est-1, est-2, paraoxonase/arylesterase, a-esterase, alpha-esterase,
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aromatic esterase
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Aryl-ester hydrolase
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neuropathy target esterase-related esterase
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NRECV
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catalytic domain of NRE
patatin-like phospholipase 7
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phenyl valerate esterase
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hydrolysis of carboxylic ester
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aryl-ester hydrolase
Acts on many phenolic esters. The reactions of EC 3.1.8.1 aryldialkylphosphatase, were previously attributed to this enzyme. It is likely that the three forms of human paraoxonase are lactonases rather than aromatic esterases [7,8]. The natural substrates of the paraoxonases are lactones [7,8], with (+/-)-5-hydroxy-6E,8Z,11Z,4Z-eicostetraenoic-acid 1,5-lactone being the best substrate [8].
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phenyl acetate + H2O
phenol + acetate
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2-naphthyl acetate + H2O
2-naphthol + acetate
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mipafox + H2O
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phenyl valerate + H2O
phenol + valerate
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additional information
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additional information
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PON1 is a lipo-lactonase which is associated with high density lipoprotein and possesses antioxidative properties
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additional information
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PON1 is a lipo-lactonase which is associated with high density lipoprotein and possesses antioxidative properties
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additional information
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does not use paraoxon as a substrate
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Ca2+
0.9 mM, the further increase of the calcium concentration does not significantly increase arylesterase activity
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aspirin
induces enzyme expression in the liver and stimulates enzymatic activity in blood plasma
di-oleoyl phosphatidylcholine
2.6 mM di-oleoyl phosphatidylcholine stimulates arylesterase activity of PON1 3.5fold
salicylic acid
induces enzyme expression in the liver and stimulates enzymatic activity in blood plasma
additional information
serum arylesterase activity is positively correlated with serum total cholesterol and low density lipoprotein and very low density lipoprotein-cholesterol concentrations
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0.014 - 0.201
phenyl acetate
0.5
2-naphthyl acetate
pH 8.0
0.014
phenyl acetate
wild type enzyme, in simulated body fluid, at pH 7.34-7.4 and 37°C
0.201
phenyl acetate
wild type enzyme, in 20 mM Tris-HCl and 0.9 mM CaCl2, at pH 8 and 25°C
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2.12
2-naphthyl acetate
pH 8.0
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UniProt
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PON1_MOUSE
355
0
39565
Swiss-Prot
Secretory Pathway (Reliability: 4 )
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52000
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recombinant catalytic domain of NRE, SDS-PAGE
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8
diminishing the pH below 8.0 in the Tris/HCl medium does not cause the activation of arylesterase
692124
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25
increasing the temperature over 25°C in the Tris/HCl medium does not cause the activation of arylesterase
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addition of di-oleoyl phosphatidylcholine to serum stabilizes PON1 activity and protects PON1 from nitrilotriacetic acid-induced inactivation
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expressed in HEK-293 cells
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medicine
PON1 overexpression is associated with decreased diabetes-induced macrophage oxidative stress, decreased diabetes development, and decreased mortality
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Aharoni, A.; Gaidukov, L.; Yagur, S.; Toker, L.; Silman, I.; Tawfik, D.S.
Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization
Proc. Natl. Acad. Sci. USA
101
482-487
2004
Homo sapiens (Q15166), Mus musculus (Q62087), Oryctolagus cuniculus (Q9BGN0)
brenda
Efrat, M.; Rosenblat, M.; Mahmood, S.; Vaya, J.; Aviram, M.
Di-oleoyl phosphatidylcholine (PC-18:1) stimulates paraoxonase 1 (PON1) enzymatic and biological activities: in vitro and in vivo studies
Atherosclerosis
202
461-469
2009
Mus musculus (P52430), Mus musculus
brenda
Lopez-Oliva, E.; Nus, M.; Agis-Torres, A.; Villaro, W.; Sanchez-Montero, J.M.; Munoz-Martinez, E.; Sanchez-Muniz, F.J.
Growth hormone improves lipoprotein concentration and arylesterase activity in mice with an atherogenic lipid profile induced by lactalbumin
Br. J. Nutr.
101
518-526
2009
Mus musculus (P52430)
brenda
Nus, M.; Sanchez-Muniz, F.J.; Gago, J.V.; Lopez-Oliva, E.; Sanchez-Montero, J.M.
Determination of rat and mice arylesterase activity using serum mimetics
Enzyme Microb. Technol.
43
252-256
2008
Mus musculus (P52430), Rattus norvegicus (P55159)
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brenda
Rozenberg, O.; Shiner, M.; Aviram, M.; Hayek, T.
Paraoxonase 1 (PON1) attenuates diabetes development in mice through its antioxidative properties
Free Radic. Biol. Med.
44
1951-1959
2008
Mus musculus (P52430), Mus musculus
brenda
Chang, P.; Long, D.; Sun, Q.; Wang, Q.; Bu, Y.; Wu, Y.
Identification and characterization of a splice variant of the catalytic domain of mouse NTE-related esterase
Gene
417
43-50
2008
Mus musculus
brenda
Jaichander, P.; Selvarajan, K.; Garelnabi, M.; Parthasarathy, S.
Induction of paraoxonase 1 and apolipoprotein A-I gene expression by aspirin
J. Lipid Res.
49
2142-2148
2008
Mus musculus (P52430), Mus musculus
brenda
Transporter Classification Database (TCDB):
1.A.6.2.6