Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.1.1.11 - pectinesterase and Organism(s) Arabidopsis thaliana and UniProt Accession Q5MFV6

for references in articles please use BRENDA:EC3.1.1.11
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.11 pectinesterase
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q5MFV6 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
pectin methylesterase, pectinesterase, pectin methyl esterase, pectinmethylesterase, pectin esterase, pme i, sal k 1, atpme3, hms-1, pmeu1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pectin methylesterase
-
At1g23200
locus name
cell wall pectin methylesterase
-
P65
-
-
-
-
PE
-
-
-
-
pectase
-
-
-
-
pectin demethoxylase
-
-
-
-
pectin methoxylase
-
-
-
-
pectin methyl esterase
pectin methylesterase
pectin methylesterase 3
-
-
pectin methylesterase31
-
pectinesterase 31
-
pectinoesterase
-
-
-
-
pectofoetidin
-
-
-
-
PME31
PME35
-
isoform
pollen specific PME
-
-
RIP
-
the RIP purified is identified as a mature form of pectin methylesterase, the purified native protein shows both pectin methylesterase and ribosome-inactivating protein activity
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pectin + n H2O = n methanol + pectate
show the reaction diagram
reaction mechanism, D157, stabilized by a hydrogen bond to R225, performs a nucleophilic attack on the ester bond of the carboxymethyl group of HGA
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
pectin pectylhydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-98-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pectin + H2O
methanol + pectate
show the reaction diagram
homogalacturonan + H2O
?
show the reaction diagram
different homogalacturonan substrates, best at pH 4.0-6.0 for enzyme BcPME
-
-
?
pectin + H2O
methanol + pectate
show the reaction diagram
pectin + n H2O
n methanol + pectate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pectin + H2O
methanol + pectate
show the reaction diagram
PME plays an important role in elongation of the pollen tube in pistil, which is essential for delivering sperms into the female gametophyte in sexual plant reproduction, regulation mechanism, overview
-
-
?
pectin + H2O
methanol + pectate
show the reaction diagram
pectin + n H2O
n methanol + pectate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aluminium
-
a toxic metal in soils that inhibits plant root elongation, can be modulated by PME activity, overexpression of PME activity leads to increases in aluminium content in the plant, which correlates to reductions in the degree of pectin methylesterification, overview
Na+
pme31 mutants are more sensitive to Na+ toxicity than the wild-type
additional information
-
the enzyme does not require salt for activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(E)-1-(2-nitroethenyl)-4-(2-propenyloxy)-benzene
-
1-(3,4-dihydroxyphenyl)-2-([1-(1-naphthyl)-1H-tetrazol-5-yl]thio)ethanone
-
1-(3,6-dichloro-9H-carbazoyl-9-yl)-3-morpholinopropan-2-ol
-
10-(hydroxymethylene)-9(10H)-phenanthrenone
-
2-(2-[4-(trifluoromethyl)phenyl]hydrazylidene)propanedinitrile
-
2-(3,4-dihydro-1(2H)-naphthalenylidene)hydrazinecarbothioamide
-
2-(3-chloro-2-fluorophenyl)-2,3-dihydroisothiazol-3-one
-
2-chloro-3-(2-methylphenoxy)-1,4-dihydro-naphthalene-1,4-dione
-
2-methoxyacridin-9-amine
-
2-[(4-chlorophenyl)sulfonyl]-2,4-dihydro-4-(2-propen-1-yl)-5-propyl-3H-pyrazol-3-one
-
3H-pyrazol-3-one
-
4-(3-hydroxy-3-methylbut-1-ynyl)benzaldehyde-2-phenylhydrazone
-
4-[1-(fur-2-oyl)pyrazol-5-yl]-5-methyl-1-phenylpyrazole
-
N'1-[2-(tert-butyl)-5-(trifluoromethyl)-pyrazolo[1,5-alpha]-pyrimidin-7-yl]-4-chlorobenzenohydrazide
-
N'6-[3,5-di-(trifluoromethyl)phenyl]-5-oxo-2,3-dihydro-5H-pyrimido-[2,1-b][1,3]thiazole-6-carbohydrazide
-
N-(1,4-dibenzoyl-5-phenyl-4,5-dihydro-1H-1,2,4-triazol-3-yl)-benzamide
-
N-(3,5-dichlorophenyl)-N'-[5-(2-furyl)-1H-pyrazol-3-yl]urea
-
N-(4-chloro-2-nitrophenyl)-N'-phenylurea
-
N-(4-methyl-2-thienyl)-N'-[4-(trifluoromethyl)phenyl]-urea
-
N-[(2-chlorobenzoyl)oxy]-2,1,3-benzoxadiazole-5-carboximidamide
-
N1-[3-(trifluoromethyl)phenyl]-3-(2-thienylthio)-propanamide
-
N4-(2-furylmethyl)-2-(2,3-dihydro-1,4-benzodioxin-2-yl)-1,3-thiazole-4-carboxamide
-
NaCl
-
activity decreases in the presence of 0.1 M NaCl and is 4times lower in 0.5 M NaCl
pectin methylesterase inhibitor 7
PMEI7, inhibits the enzyme only at pH 5.0, not at pH 6.3-7.5, re-incubating the sample at pH values where the inhibitory capacity of AtPMEI7 is not expected, fully restores AtPME3 activity. PME3 enzyme-bound complex structure analysis, stability of the AtPME3-AtPMEI7 complex at acidic and neutral pH, overview. The mechanism of competition between intramolecular and intermolecular contacts is not isolated to a single pair of residues. Changes in the protonation of amino acids at the complex interface shift the network of interacting residues between intermolecular and intramolecular. These shifts ultimately regulate the stability of the PME3-PMEI7 complex and the inhibition of the PME as a function of the pH. Analysis of the conformational dynamics of the PMEI helices reveals a consistent twist of helix alphaII, which is mostly involved in the binding of AtPME3. The dihedral space explored by helix alphaII reveals the presence of several populations at pH 7.0, with values of psi dihedral angles shifting toward regions of the Ramachandran plot that do not characterize alpha-helices. PMEI7 mutated version E68A (as the wild-type protein) is able to inhibit PME activity, whereas E75A is not
-
phenylmercuric acetate
-
polyphenon 60
PP60, commercial inhibitor mixture that contains 60% catechin (which consists of 34% (-)-epigallocatechin-3-gallate, 16.7% (-)-epigallocatechin, 8.7% (-)-epicatechin-3-gallate, 7.3% (-)-epicatechin, 2.8% (-)-gallocatechin gallate, and 0.5% (-)-catechin gallate), tannic acid, or (-)-epigallocatechin-3-gallate
-
Silver nitrate
complete inhibition
[5-(4-chlorophenyl)-3-thienyl]-(piperidino)-methanone
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.246
-
crude extract
11.98
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
compared to pH 4.0, homogalacturonans as substrates
8 - 8.5
-
in phosphate buffer
9
-
around pH 9 in glycine buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.6
-
isoelectric focusing
9.8
-
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
tube, the enzyme belongs to the group II of pectinesterases in Arabidopsis thaliana pollen
Manually annotated by BRENDA team
low expression of PME31
Manually annotated by BRENDA team
moderate to lower expression of PME31
Manually annotated by BRENDA team
gene PME34 is highly expressed in guard cells and in response to the phytohormone abscisic acid
Manually annotated by BRENDA team
low expression of PME31
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
guard cell walls concerted with the action of cell-wall enzymes, acting on the cell wall polymers for stomatal movements, regulation, overview
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PME37_ARATH
588
1
62958
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33300
-
x * 33300, mature enzyme, estimated from SDS-PAGE
35000
-
SDS-PAGE
37000
-
SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
primary structures of isozymes, structural and processing motifs, three-dimensional structure analysis, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
the enzyme contains a pro-region, role of the PRO region in PME targeting and function
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
no activity at pH 9
683188
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 55
-
incubation at 30°C has little but significant effect on enzyme activity, the incubation at 55°C abolishes over 95% of enzyme activity
85
purified enzyme, 10 min, inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, crude extract, several weeks, no significant loss of activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Ni-NTA agarose column chromatography
-
Ni-NTA His-bind resin chromatography, DEAE-Sephadex gel filtration, CM-cellulose column chromatography, and Shephacryl SH-100 gel filtration
-
recombinant His-tagged isozyme PME31 from Escherichia coli strain JM101 by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence and phylogenetic analysis
diverse isozymes, DNA sequence anaylsis, phylogenetic tree, PME transcriptomes, overview
-
DNA sequence and phylogenetic analysis
expressed in Escherichia coli strain JM101, IPTG induction at 37°C instead of 25-28°C results in approximately 10times less PME activity in the extract
-
expressed in Escherichia coli strain M15, the recombinant PME proteins (full-length and mature) do not show either PME or RIP activity
-
gene PME3, quantitative real-time RT-PCR enzyme expression analysis
gene PME6, DNA and amino acid sequence determination and analysis, recombinant expression of YFP-tagged enzyme in the seed coat and cells of the embryos, the HMS-YFP fusions does not have enough HMS activity to compensate for the hms mutation
recombinant expression of wild-type enzyme and mutants in Nicotiana benthamiana leaves via the Agrobacterium tumefaciens strain GV3101 transfection method, quantitative RT-PCR enzyme expression analysis
recombinant overexpression of His-tagged isozyme PME31 in Escherichia coli strain JM101
YFP-tagged protein expressed in host
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene PME34 is highly expressed in guard cells and in response to the phytohormone abscisic acid
isoform PME3 is induced upon infection with Botrytis cinerea and Pectobacterium carotovorum. The enzyme expression is induced upon herbivore attack as a defense mechanism
-
salt stress significantly decreases the PME31 expression, pme31 mutants expression is more affected
upon infection, Pectobacterium carotovorum and Botrytis cinerea induce in Arabidopsis a rapid expression of isoform PME3
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tian, G.W.; Chen, M.H.; Zaltsman, A.; Citovsky, V.
Pollen-specific pectin methylesterase involved in pollen tube growth
Dev. Biol.
294
83-91
2006
Arabidopsis thaliana
Manually annotated by BRENDA team
Chen, L.; Ye, D.
Roles of pectin methylesterases in pollen-tube growth
J. Integr. Plant Biol.
49
94-98
2007
Arabidopsis thaliana (O23447), Arabidopsis thaliana (O80722), Arabidopsis thaliana (Q5MFV6), Arabidopsis thaliana (Q5MFV8), Arabidopsis thaliana (Q84WM7), Arabidopsis thaliana (Q8GXA1), Arabidopsis thaliana (Q8L7Q7), Arabidopsis thaliana (Q9LSP1), Arabidopsis thaliana (Q9LY18), Arabidopsis thaliana (Q9LY19), Arabidopsis thaliana (Q9SMY6)
-
Manually annotated by BRENDA team
Pelloux, J.; Rusterucci, C.; Mellerowicz, E.J.
New insights into pectin methylesterase structure and function
Trends Plant Sci.
12
267-277
2007
Arabidopsis thaliana, Oryza sativa, Populus trichocarpa, Daucus carota (P83218)
Manually annotated by BRENDA team
De-la-Pena, C.; Badri, D.V.; Vivanco, J.M.
Novel role for pectin methylesterase in Arabidopsis: A new function showing ribosome-inactivating protein (RIP) activity
Biochim. Biophys. Acta
1780
773-783
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Mach, J.
Will remodel to suit: cellulose binding protein secreted by a parasitic nematode interacts with Arabidopsis pectin methylesterase
Plant Cell
20
2927
2008
Arabidopsis thaliana
-
Manually annotated by BRENDA team
Hewezi, T.; Howe, P.; Maier, T.R.; Hussey, R.S.; Mitchum, M.G.; Davis, E.L.; Baum, T.J.
Cellulose binding protein from the parasitic nematode Heterodera schachtii interacts with Arabidopsis pectin methylesterase: cooperative cell wall modification during parasitism
Plant Cell
20
3080-3093
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Dedeurwaerder, S.; Menu-Bouaouiche, L.; Mareck, A.; Lerouge, P.; Guerineau, F.
Activity of an atypical Arabidopsis thaliana pectin methylesterase
Planta
229
311-321
2009
Arabidopsis thaliana
Manually annotated by BRENDA team
Guenin, S.; Mareck, A.; Rayon, C.; Lamour, R.; Assoumou Ndong, Y.; Domon, J.M.; Senechal, F.; Fournet, F.; Jamet, E.; Canut, H.; Percoco, G.; Mouille, G.; Rolland, A.; Rusterucci, C.; Guerineau, F.; Van Wuytswinkel, O.; Gillet, F.; Driouich, A.; Lerouge, P.; Gutierrez, L.; Pelloux, J.
Identification of pectin methylesterase 3 as a basic pectin methylesterase isoform involved in adventitious rooting in Arabidopsis thaliana
New Phytol.
192
114-126
2011
Arabidopsis thaliana
Manually annotated by BRENDA team
Lionetti, V.; Cervone, F.; Bellincampi, D.
Methyl esterification of pectin plays a role during plant-pathogen interactions and affects plant resistance to diseases
J. Plant Physiol.
169
1623-1630
2012
Apium graveolens, Arabidopsis thaliana, Solanum nigrum, Nicotiana attenuata
Manually annotated by BRENDA team
Raiola, A.; Lionetti, V.; Elmaghraby, I.; Immerzeel, P.; Mellerowicz, E.J.; Salvi, G.; Cervone, F.; Bellincampi, D.
Pectin methylesterase is induced in Arabidopsis upon infection and is necessary for a successful colonization by necrotrophic pathogens
Mol. Plant Microbe Interact.
24
432-440
2011
Arabidopsis thaliana
Manually annotated by BRENDA team
Hongo, S.; Sato, K.; Yokoyama, R.; Nishitani, K.
Demethylesterification of the primary wall by pectin methylesterase35 provides mechanical support to the Arabidopsis stem
Plant Cell
24
2624-2634
2012
Arabidopsis thaliana
Manually annotated by BRENDA team
Yan, J.; He, H.; Fang, L.; Zhang, A.
Pectin methylesterase31 positively regulates salt stress tolerance in Arabidopsis
Biochem. Biophys. Res. Commun.
496
497-501
2018
Arabidopsis thaliana (Q9LVQ0), Arabidopsis thaliana Col-0 (Q9LVQ0)
Manually annotated by BRENDA team
L'Enfant, M.; Domon, J.M.; Rayon, C.; Desnos, T.; Ralet, M.C.; Bonnin, E.; Pelloux, J.; Pau-Roblot, C.
Substrate specificity of plant and fungi pectin methylesterases identification of novel inhibitors of PMEs
Int. J. Biol. Macromol.
81
681-691
2015
Botrytis cinerea (A0A384J5R9), Citrus sinensis (O04886), Arabidopsis thaliana (Q9LVQ0), Botrytis cinerea B05.10 (A0A384J5R9)
Manually annotated by BRENDA team
Senechal, F.; Habrylo, O.; Hocq, L.; Domon, J.M.; Marcelo, P.; Lefebvre, V.; Pelloux, J.; Mercadante, D.
Structural and dynamical characterization of the pH-dependence of the pectin methylesterase-pectin methylesterase inhibitor complex
J. Biol. Chem.
292
21538-21547
2017
Arabidopsis thaliana (O49006), Arabidopsis thaliana Col-0 (O49006)
Manually annotated by BRENDA team
Guenin, S.; Hardouin, J.; Paynel, F.; Mueller, K.; Mongelard, G.; Driouich, A.; Lerouge, P.; Kermode, A.R.; Lehner, A.; Mollet, J.C.; Pelloux, J.; Gutierrez, L.; Mareck, A.
AtPME3, a ubiquitous cell wall pectin methylesterase of Arabidopsis thaliana, alters the metabolism of cruciferin seed storage proteins during post-germinative growth of seedlings
J. Exp. Bot.
68
1083-1095
2017
Arabidopsis thaliana (O49006), Arabidopsis thaliana
Manually annotated by BRENDA team
Levesque-Tremblay, G.; Mueller, K.; Mansfield, S.D.; Haughn, G.W.
Highly methyl esterified seeds is a pectin methyl esterase involved in embryo development
Plant Physiol.
167
725-737
2015
Arabidopsis thaliana (O49298)
Manually annotated by BRENDA team
Wu, H.C.; Huang, Y.C.; Stracovsky, L.; Jinn, T.L.
Pectin methylesterase is required for guard cell function in response to heat
Plant Signal. Behav.
12
e1338227
2017
Arabidopsis thaliana (Q9M3B0), Arabidopsis thaliana
Manually annotated by BRENDA team