Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.8.4.3 - tRNA-2-methylthio-N6-dimethylallyladenosine synthase

for references in articles please use BRENDA:EC2.8.4.3
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
This bacterial enzyme binds two [4Fe-4S] clusters as well as the transferred sulfur . The enzyme is a member of the superfamily of S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes. The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover. The identity of the electron donor is not known.
Specify your search results
Select one or more organisms in this record: ?
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
The enzyme appears in viruses and cellular organisms
Reaction Schemes
N6-dimethylallyladenine37 in tRNA
+
sulfur-(sulfur carrier)
+
2
+
reduced electron acceptor
=
2-(methylsulfanyl)-N6-dimethylallyladenine37 in tRNA
+
+
(sulfur carrier)
+
+
+
electron acceptor
N6-dimethylallyladenine37 in tRNA
+
sulfur-(sulfur carrier)
+
+
reduced electron acceptor
=
2-sulfanyl-N6-dimethylallyladenine37 in tRNA
+
(sulfur carrier)
+
+
+
electron acceptor
+
2-sulfanyl-N6-dimethylallyladenine37 in tRNA
=
+
2-(methylsulfanyl)-N6-dimethylallyladenine37 in tRNA
Synonyms
2-methylthio-N-6-isopentenyl adenosine synthase, MiaB, TM0653, tRNA-i6A37 methylthiotransferase, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-(methylsulfanyl)-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor
show the reaction diagram
N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = 2-sulfanyl-N6-dimethylallyladenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor
show the reaction diagram
S-adenosyl-L-methionine + 2-sulfanyl-N6-dimethylallyladenine37 in tRNA = S-adenosyl-L-homocysteine + 2-(methylsulfanyl)-N6-dimethylallyladenine37 in tRNA
show the reaction diagram
Select items on the left to see more content.