Information on EC 2.8.3.9 - butyrate-acetoacetate CoA-transferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.8.3.9
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RECOMMENDED NAME
GeneOntology No.
butyrate-acetoacetate CoA-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
butanoyl-CoA + acetoacetate = butanoate + acetoacetyl-CoA
show the reaction diagram
butanoate, acetoacetate and their CoA thioesters are the preferred substrates, but the enzyme also acts, more slowly, on the derivatives of a number of C2 to C6 monocarboxylic acids
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-lysine fermentation to acetate and butanoate
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Lysine degradation
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pyruvate fermentation to acetone
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SYSTEMATIC NAME
IUBMB Comments
butanoyl-CoA:acetoacetate CoA-transferase
Butanoate, acetoacetate and their CoA thioesters are the preferred substrates, but the enzyme also acts, more slowly, on the derivatives of a number of C2 to C6 monocarboxylic acids.
CAS REGISTRY NUMBER
COMMENTARY hide
66231-37-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bovine
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Manually annotated by BRENDA team
SB4
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Manually annotated by BRENDA team
SB4
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Manually annotated by BRENDA team
C22
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxypropanoate + acetoacetyl-CoA
3-hydroxypropanoyl-CoA + acetoacetate
show the reaction diagram
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reaction at 8.6% the rate of butyrate
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r
3-mercaptopropionate + acetoacetyl-CoA
3-mercaptopropionyl-CoA + acetoacetate
show the reaction diagram
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reaction at 71% the rate of butyrate
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r
3-methylvinylacetate + acetoacetyl-CoA
3-methylvinylacetyl-CoA + acetoacetate
show the reaction diagram
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reaction at 44% the rate of butyrate
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r
4-hydroxybutyrate + acetoacetyl-CoA
4-hydroxybutanoyl-CoA + acetoacetate
show the reaction diagram
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reaction at 14% the rate of butyrate
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r
4-pentenoate + acetoacetyl-CoA
4-pentenoyl-CoA + acetoacetate
show the reaction diagram
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reaction at 21% the rate of acetate
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r
acetate + acetoacetyl-CoA
acetyl-CoA + acetoacetate
show the reaction diagram
acetyl-CoA + acetoacetate
acetate + acetoacetyl-CoA
show the reaction diagram
acrylate + acetoacetyl-CoA
acrylyl-CoA + acetoacetate
show the reaction diagram
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reaction at 10% the rate of butyrate
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r
butanoate + acetoacetyl-CoA
butanoyl-CoA + acetoacetate
show the reaction diagram
butanoyl-CoA + 2-methylacetoacetate
butanoate + 2-methylacetoacetyl-CoA
show the reaction diagram
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used 12% as fast as acetoacetate
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r
butanoyl-CoA + 3-oxohexanoate
butanoate + 3-oxohexanoyl-CoA
show the reaction diagram
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used 21% as fast as acetoacetate
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r
butanoyl-CoA + 3-oxopentanoate
butanoate + 3-oxopentanoyl-CoA
show the reaction diagram
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used 60% as fast as acetoacetate
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r
butanoyl-CoA + acetoacetate
butanoate + acetoacetyl-CoA
show the reaction diagram
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r
butyrate + acetyl-CoA
butyryl-CoA + acetate
show the reaction diagram
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reaction at 56% the rate of acetoacetate, reverse reaction at 86% the rate of acetoacetyl-CoA
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r
crotonate + acetoacetyl-CoA
crotonyl-CoA + acetoacetate
show the reaction diagram
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reaction at 16% the rate of butyrate
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r
crotonyl-CoA + acetate
crotonate + acetyl-CoA
show the reaction diagram
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reaction at 4% the rate of acetoacetylCoA
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r
DL-2-hydroxybutyrate + acetoacetyl-CoA
2-hydroxybutyryl-CoA + acetoacetate
show the reaction diagram
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reaction at 19% the rate of butyrate
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r
DL-2-hydroxypentanoate + acetoacetyl-CoA
2-hydroxypentanoyl-CoA + acetoacetate
show the reaction diagram
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reaction at 7.9% the rate of butyrate
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r
DL-3-hydroxybutyrate + acetoacetyl-CoA
3-hydroxybutyryl-CoA + acetoacetate
show the reaction diagram
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reaction at 30% the rate of butyrate
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r
DL-3-hydroxybutyrate + acetyl-CoA
3-hydroxybutyryl-CoA + acetate
show the reaction diagram
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reaction at 9% the rate of acetoacetate
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r
epsilon-acetyl-CoA + acetoacetate
acetate + acetoacetyl-CoA
show the reaction diagram
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reaction at 1% the rate of acetylCoA
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r
formate + acetoacetyl-CoA
formyl-CoA + acetoacetate
show the reaction diagram
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reaction at 7% the rate of acetate
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r
isobutyrate + acetoacetyl-CoA
isobutyryl-CoA + acetoacetate
show the reaction diagram
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reaction at 19% the rate of butyrate
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r
isopentanoate + acetoacetyl-CoA
isopentanoyl-CoA + acetoacetate
show the reaction diagram
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reaction at 8.3% the rate of butyrate
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r
monochloroacetate + acetoacetyl-CoA
monochloroacetyl-CoA + acetoacetate
show the reaction diagram
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reaction at 5.4% the rate of butyrate
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r
octanoate + acetoacetyl-CoA
octanoyl-CoA + acetoacetate
show the reaction diagram
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reaction at 3% the rate of acetate
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r
pentanoate + acetoacetyl-CoA
pentanoyl-CoA + acetoacetate
show the reaction diagram
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reaction at 19% the rate of butyrate
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r
propionate + acetoacetyl-CoA
propionyl-CoA + acetoacetate
show the reaction diagram
vinylacetate + acetoacetyl-CoA
vinylacetyl-CoA + acetoacetate
show the reaction diagram
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best substrate
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r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
butanoate + acetoacetyl-CoA
butanoyl-CoA + acetoacetate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activation at 6 mM CaCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
K+
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activation at 0.08 M KCl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Li+
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activation at 0.2 M LiCl, inhibitions at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Mg2+
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activation at 12 mM MgCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Mn2+
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activation at 1.2 mM MnCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Na+
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activation at 0.17 M NaCl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
NH4+
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activation at 0.08 M NH4Cl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
epsilon-Acetyl-CoA
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p-chloromercuribenzoate
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additional information
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partially inactivated by acyl-CoA substrates in absence of carboxylic acid substrates
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Tris-HCl
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activation at 0.25 M, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
250
3-hydroxypropionate
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pH 7.5, 25C
29
3-Mercaptopropionate
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pH 7.5, 25C
42
3-Methylvinylacetate
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pH 7.5, 25C
33
4-hydroxybuyrate
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pH 7.5, 25C
24
acetate
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pH 7.5, 25C
1.1 - 1.8
acetoacetate
0.0034 - 0.035
acetoacetyl-CoA
0.135 - 0.33
acetyl-CoA
40
Acrylate
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pH 7.5, 25C
0.8
Butanoate
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pH 7.5, 25C
0.023 - 0.083
Butyryl-CoA
2
crotonate
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pH 7.5, 25C
0.167
crotonyl-CoA
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pH 7.0, 25C, cosubstrate acetoacetate
49
DL-2-hydroxybuyrate
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pH 7.5, 25C
21
DL-2-Hydroxypentanoate
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pH 7.5, 25C
44
DL-3-hydroxybutyrate
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pH 7.5, 25C
6.7
epsilon-Acetyl-CoA
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pH 8.1, 24C
14
Isobutyrate
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pH 7.5, 25C
21
Isopentanoate
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pH 7.5, 25C
29
Monochloroacetate
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pH 7.5, 25C
7
pentanoate
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pH 7.5, 25C
3.2
Propionate
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pH 7.5, 25C
0.205
propionyl-CoA
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pH 7.0, 25C, cosubstrate acetoacetate
17
Vinylacetate
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pH 7.5, 25C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.6
epsilon-Acetyl-CoA
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
117
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fraction B
160
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fraction A
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
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maximally active from pH 6.6 to pH 7.9
additional information
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active over a wide pH range
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90000
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gel electrophoresis
97000
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gel filtration
97800
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sedimentation equilibrium centrifugation
98000
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SDS-PAGE
99000
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gel filtration
108000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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1 h, at 30C, most stable, about 20% loss of activity at pH 6.0 and 8.0
643838
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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1 h, in potassium phosphate buffer, pH 6.5-7.0, most stable, about 20% loss of activity at pH 6.0 and 8.0
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified enzyme is very stable, considerably less stable in Tris-HCl than in phosphate buffer
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C 0.0062 mg/ml protein, 10 mM phosphate buffer, pH 7.0, half-life of 12 days when thawed and refrozen 5 times
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-15C, 3.1 mg protein/ml, 20 mM potassium phosphate, pH 7.0, retains full activity during storage for 1 year
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4C, 0.0062 mg/ml protein, 10 mM phosphate buffer, pH 7.0, half-life of about 7 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (226 entries)
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