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Information on EC 2.8.3.8 - acetate CoA-transferase and Organism(s) Acetobacter aceti and UniProt Accession B3EY95

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.3 CoA-transferases
                2.8.3.8 acetate CoA-transferase
IUBMB Comments
The enzyme belongs to family I of CoA-transferases, which operate with a ping-pong kinetic mechanism. The reaction takes place in two half-reactions and involves the formation of a CoA thioester intermediate with a glutamate residue. Unlike EC 2.8.3.9, butyrate---acetoacetate CoA-transferase, this enzyme exhibits maximal activity using acetate as the CoA acceptor. Substrate range depends on the specific enzyme. Typical substrates include butanoyl-CoA and pentanoyl-CoA.
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This record set is specific for:
Acetobacter aceti
UNIPROT: B3EY95
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Word Map
The taxonomic range for the selected organisms is: Acetobacter aceti
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
butyryl-coa:acetate coa-transferase, butyryl-coa acetate coa-transferase, acetate:succinate coa-transferase, butyryl-coenzyme a coa transferase, acetate coa-transferase, acyl-coa:acetate coa-transferase, acetoacetate:acetate coa-transferase, succinyl-coa:acetate coa transferase, butyryl coa:acetate coa transferase, butyryl coenzyme a transferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
succinyl-CoA:acetate CoA-transferase
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succinyl-coenzyme A:acetate CoA-transferase
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acetate coenzyme A-transferase
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butyryl CoA:acetate CoA transferase
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butyryl coenzyme A transferase
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coenzyme A-transferase, acetate
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succinyl-CoA:acetate CoA transferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:acetate CoA-transferase
The enzyme belongs to family I of CoA-transferases, which operate with a ping-pong kinetic mechanism. The reaction takes place in two half-reactions and involves the formation of a CoA thioester intermediate with a glutamate residue. Unlike EC 2.8.3.9, butyrate---acetoacetate CoA-transferase, this enzyme exhibits maximal activity using acetate as the CoA acceptor. Substrate range depends on the specific enzyme. Typical substrates include butanoyl-CoA and pentanoyl-CoA.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-35-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetate + succinyl-CoA
succinate + acetyl-CoA
show the reaction diagram
5.1% activity compared to succinate
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-
r
succinate + acetyl-CoA
acetate + succinyl-CoA
show the reaction diagram
100% activity
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r
additional information
?
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less than 1% activity with acetoacetate, propionate, D-malate, fumarate, L-malate, formate, oxaloacetate, DL-methylsuccinate, and glutarate. No activity is detected with dethiaacetyl-CoA, glycolate, glyoxylate, oxalate, trifluoroacetate, DL-lactate, L-lactate, malonate, pyruvate, maleate, butyrate, D-tartrate, L-tartrate, alpha-ketoglutarate, citrate, or DL-isocitrate
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?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
110
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
290
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029 - 2600
acetate
0.7 - 52
acetyl-CoA
0.03 - 79
succinate
0.074 - 9
succinyl-CoA
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1600
acetate
wild type enzyme, in 50 mM potassium phosphate (pH 8.0), 100 mM potassium chloride, at 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
succinyl-CoA:acetate CoA-transferase is an acetic acid resistance factor (AarC) with a role in a variant citric acid cycle
physiological function
the enzyme is an acetic acid resistance factor that is required for acetate resistance
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SCACT_ACEAC
505
0
54826
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 1.7-2.0 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M sodium cacodylate (pH 6.5), and 25 mM 2-mercaptoethanol
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E294A
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
E435A
insoluble mutant
E435D
the mutant shows almost wild type activity
E435Q
insoluble mutant
R228E
the mutant has a specific defect in its ability to bind both carboxylate substrates succinyl-CoA and acetyl-CoA and does not use 4-aminobutyrate as substrate
S71A
the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, dye-affinity chromatography, ion-exchange column chromatography, and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mullins, E.A.; Kappock, T.J.
Crystal structures of Acetobacter aceti succinyl-coenzyme A (CoA):acetate CoA-transferase reveal specificity determinants and illustrate the mechanism used by class I CoA-transferases
Biochemistry
51
8422-8434
2012
Acetobacter aceti (B3EY95)
Manually annotated by BRENDA team