Information on EC 2.8.3.12 - glutaconate CoA-transferase

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The expected taxonomic range for this enzyme is: Firmicutes

EC NUMBER
COMMENTARY hide
2.8.3.12
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RECOMMENDED NAME
GeneOntology No.
glutaconate CoA-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + (E)-glutaconate = acetate + glutaconyl-1-CoA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Butanoate metabolism
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glutamate and glutamine metabolism
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L-glutamate degradation V (via hydroxyglutarate)
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Microbial metabolism in diverse environments
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Styrene degradation
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:(E)-glutaconate CoA-transferase
Glutarate, (R)-2-hydroxyglutarate, propenoate and propanoate, but not (Z)-glutaconate, can also act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
79078-99-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NCIB 10672
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Manually annotated by BRENDA team
HB 25
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Manually annotated by BRENDA team
HB 25
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Manually annotated by BRENDA team
no activity in Clostridium tetanomorphum
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-
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Manually annotated by BRENDA team
no activity in Clostridium tetanomorphum H1
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + (E)-glutaconate
acetate + glutaconyl-1-CoA
show the reaction diagram
acetyl-CoA + (R)-2-hydroxyglutarate
acetate + (R)-2-hydroxyglutaryl-1-CoA
show the reaction diagram
acetyl-CoA + (R)-2-hydroxyglutarate
acetate + (R)-2-hydroxyglutaryl-CoA
show the reaction diagram
acetyl-CoA + acrylate
acrylyl-CoA + acetate
show the reaction diagram
-
poor substrate
-
-
?
acetyl-CoA + crotonate
crotonyl-CoA + acetate
show the reaction diagram
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poor substrate
-
-
?
acetyl-CoA + isocrotonate
isocrotonyl-CoA + acetate
show the reaction diagram
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poor substrate
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-
?
glutaconyl-CoA + (R)-2-hydroxyglutarate
glutaconate + (R)-2-hydroxyglutaryl-CoA
show the reaction diagram
glutaconyl-CoA + (S)-2-hydroxyglutarate
glutaconate + (S)-2-hydroxyglutaryl-CoA
show the reaction diagram
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at 60% of the rate with (R)-isomer or glutarate
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-
?
glutaconyl-CoA + 3-hydroxyglutarate
glutaconate + 3-hydroxyglutaryl-CoA
show the reaction diagram
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at 20% of the rate with glutarate
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-
?
glutaconyl-CoA + adipate
glutaconate + adipyl-CoA
show the reaction diagram
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at 60% of the rate with glutarate
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?
glutaconyl-CoA + butyrate
butyryl-CoA + glutaconate
show the reaction diagram
-
-
-
-
?
glutaconyl-CoA + glutarate
glutaconate + glutaryl-CoA
show the reaction diagram
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very good substrate
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?
glutaconyl-CoA + propionate
propanoyl-CoA + glutaconate
show the reaction diagram
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moderate substrate
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-
?
glutaryl-CoA + acetate
glutarate + acetyl-CoA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + (E)-glutaconate
acetate + glutaconyl-1-CoA
show the reaction diagram
-
involved in glutamate fermentation via hydroxyglutarate pathway, activates glutaconate to glutaconyl-CoA prior to glutaconate decarboxylation
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?
acetyl-CoA + (R)-2-hydroxyglutarate
acetate + (R)-2-hydroxyglutaryl-CoA
show the reaction diagram
additional information
?
-
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not involved in the dehydration of (R)-2-hydroxyglutarate to glutaconate
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-hydroxymercuribenzoate
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0.2 mM, at 37C, 2 min, 80% loss of activity, 2-mercaptoethanol partially restores activity
additional information
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not inhibited by 2 mM iodoacetate, 2 mM iodoacetamide or 1 mM 5,5-dithiobis(2-nitrobenzoate)
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5
(R)-2-hydroxyglutarate
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cosubstrate glutaconyl-CoA
0.035
(R)-2-hydroxyglutaryl-1-CoA
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pH 7, 37C, cosubstrate acetate
14
(S)-2-Hydroxyglutarate
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cosubstrate glutaconyl-CoA
13
3-hydroxyglutarate
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cosubstrate glutaconyl-CoA
26
acetate
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cosubstrate glutaconyl-CoA
0.17
acetyl-CoA
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cosubstrate glutaconate
10
Acrylate
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cosubstrate acetyl-CoA
8
adipate
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cosubstrate glutaconyl-CoA
150
Butyrate
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cosubstrate glutaconyl-CoA
500
crotonate
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cosubstrate acetyl-CoA
0.2
glutaconate
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pH 7, 25C, cosubstrate acetyl-CoA
0.017
glutaconyl-CoA
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cosubstrate glutarate
0.7
Glutarate
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cosubstrate glutaconyl-CoA
0.015 - 0.064
glutaryl-CoA
100
isocrotonate
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cosubstrate acetyl-CoA
16
Propionate
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cosubstrate glutaconyl-CoA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
39 - 140
glutaryl-CoA
additional information
additional information
Acidaminococcus fermentans
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 7.4
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about 70% of maximal activity at pH 5.6 and 7.4
8.5
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about 10% of maximal activity at pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
260000
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heterooctamer GCT
275000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
betaE54D mutant enzyme, expressed in Escherichia coli
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native and recombinant enzyme, expressed in Escherichia coli
Q59111 and Q59112
recombinant GCT, expressed in Escherichia coli, sitting-drop vapour diffusion method
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, crystalline suspension in ammonium sulfate, 2 years, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
betaE54D mutant enzyme and 65 kDa GctF fusion protein, expressed in Escherichia coli
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native and recombinant enzyme, expressed in Escherichia coli
Q59111 and Q59112
recombinant GCT, expressed in Escherichia coli
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recombinant wild-type and betaE54D mutant enzyme, expressed in Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GCT expression in Escherichia coli
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gctAB genes encoding the two subunits GctA and GctB are cloned, sequenced and overexpressed together in Escherichia coli DH5alpha, the two genes are located at the beginning of the hydroxyglutarate operon, GctA: 320 amino acids protein, GctB: 266 amino acids protein
Q59111 and Q59112
overexpression of betaE54N and betaE54A mutant enzyme in Escherichia coli, fusion of the genes gctA and gctB, encoding the 2 subunits of Gct, yields GctF, which is expressed in Escherichia coli as a 65 kDa protein with 30% of wild-type activity; overexpression of wild-type and betaE54D mutant enzyme in Escherichia coli
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overexpression of wild-type and betaE54D mutant enzyme in Escherichia coli
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wild-type and mutant genes gctA and gctB encoding the two subunits GctA and GctB are cloned and expressed together in Escherichia coli XL1-Blue
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E64A
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mutation in the subunit GctB, 30% as active as wild-type enzyme
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