Information on EC 2.8.2.9 - tyrosine-ester sulfotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.9
-
RECOMMENDED NAME
GeneOntology No.
tyrosine-ester sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + L-tyrosine methyl ester = adenosine 3',5'-bisphosphate + L-tyrosine methyl ester 4-sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:L-tyrosine-methyl-ester sulfotransferase
Phenols and organic hydroxylamines can act as acceptors (cf. EC 2.8.2.1 aryl sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
9055-56-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. bacillaris, W10BSmL, aplastidic mutant
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phospho-R-(-)-1,2,3,4-tetrahydro-1-naphthol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
stereochemical configuration is important, the other enantiomer acts as inhibitor
-
-
?
3'-phospho-S-(-)-2-methyl-1-phenylpropanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
stereochemical configuration is important, the other enantiomer acts as inhibitor
-
-
?
3'-phosphoadenosine 5'-phosphosulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + (R)-(-)-1,2,3,4-tetrahydro-1-naphthol
adenosine 3',5'-bisphosphate + (R)-(-)-1,2,3,4-tetrahydro-1-naphthyl sulfate
show the reaction diagram
-
activity is abserved with wild-type enzyme and mutant enzymes F77A, F138A and Y236A
-
-
?
3'-phosphoadenylylsulfate + (S)-(+)-1,2,3,4-tetrahydro-1-naphthol
adenosine 3',5'-bisphosphate + (S)-(+)-1,2,3,4-tetrahydro-1-naphthyl sulfate
show the reaction diagram
-
mutant enzyme F77A and F138A show activity, wild-type enzyme and mutant enzyme Y236A show no activity
-
-
?
3'-phosphoadenylylsulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 2-chloro-4-nitrophenol
adenosine 3',5'-bisphosphate + 2-chloro-4-nitrophenyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 2-chlorophenol
adenosine 3',5'-bisphosphate + 2-chlorophenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 2-cyanoethyl-N-hydroxythioacetamide
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 2-cyanoethyl-N-hydroxythioacetimidate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 2-fluoro-4-nitrophenol
adenosine 3',5'-bisphosphate + 2-fluoro-4-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-naphthol
adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 2-naphthylamine
adenosine 3',5'-bisphosphate + 2-naphthylamine sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3-chlorophenol
adenosine 3',5'-bisphosphate + 3-chlorophenyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 3-fluoro-4-nitrophenol
adenosine 3',5'-bisphosphate + 3-fluoro-4-nitrophenyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-methoxy-4-hydroxyphenylglycol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 4-chloro-N-hydroxyaniline
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 4-chlorophenol
adenosine 3',5'-bisphosphate + 4-chlorophenyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 4-methoxyphenol
adenosine 3',5'-bisphosphate + 4-methoxyphenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-methylumbelliferol
adenosine 3',5'-bisphosphate + 4-methylumbelliferyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 5-hydroxytryptamine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 5-hydroxytryptophol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 9-hydroxy-N-2-fluorenylacetamide
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + Ala-Tyr
adenosine 3',5'-bisphosphate + Ala-(O-sulfo)Tyr
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + Asp-Arg-Asp-Tyr-Met-Gly
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + caerulein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
nonsulfated
-
-
?
3'-phosphoadenylylsulfate + cholecystokinin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
nonsulfated
-
-
?
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + epinephrine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + GlyTyr
adenosine 3',5'-bisphosphate + Gly-(O-sulfo)Tyr
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + hydrocortisone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + L-4-hydroxyphenylglycine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + L-tyrosine
adenosine 3',5'-bisphosphate + tyrosine O4-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + L-tyrosine allyl ester
adenosine 3',5'-bisphosphate + tyrosine allyl ester 4-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + L-tyrosine amide
adenosine 3',5'-bisphosphate + O-sulfo-L-tyramide
show the reaction diagram
3'-phosphoadenylylsulfate + L-tyrosine benzyl ester
adenosine 3',5'-bisphosphate + tyrosine benzyl ester 4-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + L-tyrosine ethyl ester
adenosine 3',5'-bisphosphate + tyrosine ethyl ester 4-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + L-tyrosine methyl ester
adenosine 3',5'-bisphosphate + L-tyrosine methyl ester 4-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + L-tyrosine tert-butyl ester
adenosine 3',5'-bisphosphate + tyrosine tert-butyl ester 4-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + methylphenol
adenosine 3',5'-bisphosphate + methylphenyl sulfate
show the reaction diagram
-
3-methylphenol or 4-methylphenol
-
-
?
3'-phosphoadenylylsulfate + N-acetyl-L-tyrosine ethyl ester
adenosine 3',5'-bisphosphate + N-acetyl-L-tyrosine O4-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N-acetylserotonin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N-ethyl-N-hydroxyaniline
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N-hydroxy-2-aminofluorene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + N-hydroxy-N-ethylaniline
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N-hydroxy-N-methylaniline
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N-hydroxy-N-n-propylaniline
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + N-hydroxyaniline
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + nitrophenol
adenosine 3',5'-bisphosphate + nitrophenyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + phenol
adenosine 3',5'-bisphosphate + phenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + quercetin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + serotonin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + tert-butoxycarbonyl-Asp-Arg-Asp-Tyr-Met-Gly
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + tert-butoxycarbonyl-Asp-Tyr
adenosine 3',5'-bisphosphate + tert-butoxycarbonyl-Asp-Tyr-O4-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + tert-butoxycarbonyl-Asp-Tyr-Met
adenosine 3',5'-bisphosphate + tert-butoxycarbonyl-Asp-Tyr-(O4-sulfate)-Met
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + tert-butoxycarbonyl-Asp-Tyr-Met-Gly
adenosine 3',5'-bisphosphate + tert-butoxycarbonyl-Asp-Tyr-(O4-sulfate)-Met-Gly
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + tert-butoxycarbonyl-Asp-Tyr-Met-Gly-Trp
adenosine 3',5'-bisphosphate + tert-butoxycarbonyl-Asp-Tyr-(O4-sulfate)-Met-Gly-Trp
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + tert-butoxycarbonyl-Asp-Tyr-Met-Gly-Trp-Met
adenosine 3',5'-bisphosphate + tert-butoxycarbonyl-Asp-Tyr-(O4-sulfate)-Met-Gly-Trp-Met
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + tert-butoxycarbonyl-Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2
adenosine 3',5'-bisphosphate + tert-butoxycarbonyl-Asp-Tyr-(O4-sulfate)-Met-Gly-Trp-Met-Asp-Phe-NH2
show the reaction diagram
-
i.e. Boc-CCK-8 ns
-
-
?
3'-phosphoadenylylsulfate + tert-butoxycarbonylcholecystokinin-octapeptide
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + tyramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + TyrGly
adenosine 3',5'-bisphosphate + 4O-sulfo-Tyr-Gly
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine 3',5'-bisphosphate
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
-
activates below 0.1 M, inhibits at higher concentrations
MnCl2
-
activates at 0.2 mM
NaCl
-
activates below 0.1 M, inhibits at higher concentrations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-naphthaldehyde
-
reversible
1-naphthoic acid
-
-
1-naphthylacetic acid
-
competitive with respect to 1-napththalenemethanol
2'-O-[(R)-Formyl(adenin-9-yl)methyl]-(S)-glyceraldehyde 3'-triphosphate
-
ATPDA, irreversible, inhibition prevented by either adenosine 3,5'-diphosphate or 3'-phosphoadenylylsulfate, binds to Lys65 and Cys66 at the PAPS binding site
-
2,6-Dichloro-4-nitrophenol
2-acetylaminofluorene
-
increases and/or alters the inhibitory effect of metal ions, relationship between inhibition by heavy metal ions and dietary 2-acetylaminofluorene, overview
2-chloro-4-nitrophenol
-
-
2-Chloro-4-nitrophenyl sulfate
-
product inhibition
2-naphthaldehyde
-
reversible
2-naphthoic acid
-
reversible
2-naphthol
-
potent product inhibitor
2-naphthylacetic acid
-
competitive with respect to 1-napththalenemethanol
3'-phosphoadenylylsulfate
-
-
4-chloro-N-hydroxyaniline
4-chloromercuribenzoate
-
-
adenosine 3',5'-bisphosphate
Adenosine 5'-triphosphate
-
-
Aryl sulfates
-
-
-
Benzoic acid
-
competitive with respect to 1-napththalenemethanol
Cd2+
-
strong inhibition in vitro and in vivo, 50% inhibition of purified enzyme at about 0.0025 mM, 50% inhibition in liver cytosol at about 0.025 mM
Cu2+
-
elevated copper levels in the diet result in reduced enzyme levels in rat liver, strong inhibition in vitro and in vivo, 50% inhibition of purified enzyme at about 0.0025 mM, 50% inhibition in liver cytosol at about 0.025 mM
iodoacetamide
-
pH 7.5, 30C, inhibition prevented by presence of 3'-phosphate 5'-sulfatophosphate but not by L-tyrosine methyl ester
iodoacetate
-
-
KCl
-
activates below 0.1 M, inhibits at higher concentrations
N-Hydroxy-2-acetylaminofluorene
-
N-OH-2AAF, formation of AST IV:2AAF adducts leads to irreversible inactivation
N-hydroxy-2-aminofluorene
-
N-OH-2AF, causes inhibition under reaction conditions where oxidative degradation occurs
N-hydroxyaniline
-
causes inhibition under reaction conditions where oxidative degradation occurs, activity rapidly decreases to less than 10% of the initial activity
N-n-butyl-N-hydroxyaniline
-
competitive, reversible
NaCl
-
activates below 0.1 M, inhibits at higher concentrations
naproxen
-
competitive with respect to 1-napththalenemethanol
o-Iodosobenzoate
-
-
Pentachlorophenol
-
-
phenol
-
poor inhibitor of the reverse reaction
phenylacetic acid
-
competitive with respect to 1-naphthalenemethanol
Phenylarsine oxide
-
effective inhibitor
R-(+)-2-methyl-1-phenylpropanol
-
competitive, reversible inhibitor, stereochemical configuration is important, the other enantiomer acts as substrate
-
S-(+)-1,2,3,4-tetrahydro-1-naphthol
-
competitive, reversible inhibitor, stereochemical configuration is important, the other enantiomer acts as substrate
salicylic acid
-
competitive with respect to 1-napththalenemethanol
thiol reagents
-
-
Triton X-100
-
-
Tyrosine O4-sulfate
-
uncompetitive to L-tyrosine
Zn2+
-
indirect inhibition mechanism, strong inhibition in vitro and in vivo, 50% inhibition of purified enzyme at about 0.0025 mM, 50% inhibition in liver cytosol at about 0.025 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activates, degree of activation is more marked with preparations previously stored at 0C or -10C
5,5'-dithiobis(2-nitrobenzoate)
-
low concentrations lead to increase of activity at a physiological pH, higher concentrations lead to complete inactivation
Diamide
-
low concentrations lead to increase of activity at a physiological pH, higher concentrations lead to complete inactivation
dithiothreitol
-
activates, degree of activation is more marked with preparations previously stored at 0C or -10C
glutathione
-
activates, degree of activation is more marked with preparations previously stored at 0C or -10C
glutathione disulfide
-
leads to the formation of internal disulfide bonds between Cys66 and Cys232, activates the enzyme and allows it to function at a pH optimum in the physiological range
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012 - 1.4
(R)-(-)-1,2,3,4-tetrahydro-1-naphthol
0.5 - 1.8
(S)-(+)-1,2,3,4-tetrahydro-1-naphthol
0.26
2-Chloro-4-nitrophenyl sulfate
-
pH 7, 25C
0.5
2-chlorophenol
-
pH 7, 37C
6.9
2-cyanoethyl-N-hydroxythioacetamide
-
pH 5.5, 37C
0.1 - 0.37
2-naphthol
1.1
2-Naphthylamine
-
pH 9, 25C
0.00012 - 41
3'-phosphoadenylylsulfate
0.39 - 0.4
3-chlorophenol
0.2
3-methoxy-4-hydroxyphenylglycol
0.416 - 0.44
3-Nitrophenol
0.026
4-chloro-N-hydroxyaniline
-
pH 7, 37C
0.34 - 0.55
4-Chlorophenol
1.4
4-methoxyphenol
-
pH 5.5, 37C
1.2
4-Methylphenol
-
pH 5.5, 37C
0.00775 - 1.25
4-nitrophenol
0.21
5-hydroxytryptophol
-
pH 5.5, 37C
26.4
adenosine 3',5'-bisphosphate
-
pH 7, 25C
0.000008
adenosine 3'-phosphate 5'-sulfophosphate
-
pH 7.5, 37C
0.35
AlaTyr
-
pH 7, 37C
0.16 - 0.41
dopamine
0.43
epinephrine
-
pH 5.5, 37C
0.0021
estrone
-
pH 9, 25C
2.5
GlyTyr
-
pH 7, 37C
0.012
hydrocortisone
-
pH 7, 25C
0.714
L-4-hydroxyphenylglycine
-
pH 7, 37C
0.033
L-tyrosine
-
pH 7, 37C
0.133
L-tyrosine benzyl ester
-
pH 7, 37C
0.37
L-tyrosine ethyl ester
-
pH 7, 37C
0.15 - 0.3
L-tyrosine methyl ester
0.08
L-tyrosine tert-butyl ester
-
pH 7, 37C
1.5
N-acetylserotonin
-
pH 5.5, 37C
0.65
N-ethyl-N-hydroxyaniline
-
pH 7, 37C
0.65
N-hydroxy-N-ethylaniline
-
pH 7, 37C
0.119 - 0.12
N-hydroxy-N-methylaniline
0.161
N-hydroxy-N-n-propylaniline
-
pH 7, 37C
0.23
N-hydroxyaniline
-
pH 7, 37C
0.92
phenol
-
pH 5.5, 37C
0.00085
quercetin
-
pH 7, 25C
0.14
t-butyloxycarbonyl-cholecystokinin-octapeptide
-
pH 7.4, 37C
0.833
Tyr-Gly
-
pH 7, 37C
0.46
tyramine
-
pH 5.5, 37C
0.91
tyrosine methyl ester
0.62
tyrosine methylester
-
pH 8, 25C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0233
2-cyanoethyl-N-hydroxythioacetamide
-
pH 5.5, 37C
0.233
2-cyanoethyl-N-hydroxythioacetimidate
-
pH 5.5, 37C
0.7
2-naphthol
-
pH 5.5, 37C
0.45
3-chlorophenol
-
pH 5.5, 37C
0.183
3-methoxy-4-hydroxyphenolglycol
-
pH 5.5, 37C
0.183
3-methylphenol
-
pH 5.5, 37C
0.8
3-Nitrophenol
-
pH 5.5, 37C
0.3 - 0.317
4-Chlorophenol
0.45
4-methoxyphenol
-
pH 5.5, 37C
0.02
4-Methylphenol
-
pH 5.5, 37C
0.517
4-nitrophenol
-
pH 5.5, 37C
0.14
5-hydroxytryptophol
-
pH 5.5, 37C
0.07
dopamine
-
pH 5.5, 37C
0.122
epinephrine
-
pH 5.5, 37C
0.3
N-acetylserotonin
-
pH 5.5, 37C
0.03
phenol
0.0467
tyramine
-
pH 5.5, 37C
0.233
tyrosine methyl ester
-
pH 5.5, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029
1-naphthaldehyde
-
pH 7, 37C
0.016
1-naphthoic acid
-
pH 7, 37C
0.2
1-naphthylacetic acid
-
pH 7, 37C
0.09
2'-O-[(R)-Formyl(adenin-9-yl)methyl]-(S)-glyceraldehyde 3'-triphosphate
-
pH 7, 37C
-
0.089
2-naphthaldehyde
-
pH 7, 37C
0.041
2-naphthoic acid
-
pH 7, 37C
0.41
2-naphthylacetic acid
-
pH 7, 37C
0.02
adenosine 3',5'-bisphosphate
-
pH 7, 37C
0.28
Benzoic acid
-
pH 7, 37C
0.066
N-n-butyl-N-hydroxyaniline
-
pH 7, 37C
0.13
naproxen
-
pH 7, 37C
1.1
phenylacetic acid
-
pH 7, 37C
0.067
salicylic acid
-
pH 7, 37C
0.5
Tyrosine O4-sulfate
-
pH 7, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.104
-
recombinant enzyme
0.8 - 1
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
two optima: pH 6 and pH 9.7, reduced enzyme activity with 2-naphthylamine as substrate
6.8
-
assay at, forward direction
7.6
-
assay at, reverse direction
8
-
oxidized enzyme
9.7
-
two optima: pH 6 and pH 9.7, reduced activity with 2-naphthylamine as substrate
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
-
3 fractions with different pI: 6.6, 5.8 and 5.3
6.6
-
3 fractions with different pI: 6.6, 5.8 and 5.3
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
the activity of aryl sulfotransferase IV increases 3fold to 8fold in lungs treated with more than 95% O2. However, hyperoxic exposure does not change the mRNA and protein levels of aryl sulfotransferase IV in lungs. Oxidative regulation occurs at the level of protein modification
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
-
gel filtration
61000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 33500, SDS-PAGE
monomer
-
1 * 26000, SDS-PAGE
additional information
-
oxidative regulation of aryl sulfotransferase IV is not caused by dimer-monomer status change
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
oxidative regulation occurs at the level of protein modification. Active site Cys chemical modification can regulate AST IV activity both in vivo and in vitro
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SULT1D1 complexed with 3'-phosphoadenosine-5'-phosphate plus 4-nitrophenol or 3'-phosphoadenosine-5'-phosphate plus 1-naphthol, hanging drop vapor diffusion method, using 16% (w/v) PEG 10000, 10 mM dithiothreitol, and 100 mM Bis-Tris, pH 5.5
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SULT1D1 complexed with donor substrate 3''-phosphoadenosine 5''-phosphosulfate and accepter substrate p-nitrophenol, hanging drop vapor diffusion method, using 50 mM Tris-HCl (pH 7.9), 150 mM NaCl, 10 mM dithiothreitol, 16% (w/v) PEG 10000, 100 mM Bis-Tris (pH 5.5), 25% (v/v) glycerol
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
activity strongly decreases above pH 7
644823
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
instable to freezing
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acetone
-
5% acetone is not inhibitory
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C, stable for 6 months in sodium acetate buffer, pH 5.5, 0.25 M sucrose, 10% glycerol, 1 mM dithiothreitol at protein concentration of 1 mg/ml
-
4C, 10 mM potassium phosphate, pH 6.8, 250 mM sucrose and 0.02% w/v NaN3, no more than 10% loss of activity after 5 months
-
4C, sodium phosphate buffer, pH 7.0, 0.25 M sucrose, 5 mM 2-mercaptoethanol, 3 mM NaN3, 5% loss of activity per week at protein concentration between 0.5 and 1 mg/ml
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
aryl sulfotransferase IV
-
cloned and expressed in Escherichia coli
-
homogeneity
-
homogeneity, 2 isoforms
-
wild-type and mutant enzymes
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HAST4; HAST4v, variant of HAST4
rat enzyme expressed in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C232S
-
mutant is catalytically active
C66S
-
mutant is catalytically active, can not be activated by glutathione disulfide
F138A
-
25fold increase in KM-value for (R)-(-)-tetrahydro-1-naphthol. Unlike the wild-type enzyme the mutant enzyme is also active with (S)-(+)-1,2,3,4-tetrahydro-1-naphthol
F77A
-
83.3fold increase in KM-value for (R)-(-)-tetrahydro-1-naphthol,unlike the wild-type enzyme the mutant enzyme is also active with (S)-(+)-1,2,3,4-tetrahydro-1-naphthol
G208R
-
same pattern of specific activities as wild-type enzyme
H104A
-
no enzymatic activity
Y236A
-
116.7fold increase in KM-value for (R)-(-)-tetrahydro-1-naphthol