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Information on EC 2.8.2.8 - [heparan sulfate]-glucosamine N-sulfotransferase and Organism(s) Rattus norvegicus and UniProt Accession Q02353
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2.8.2.8 [heparan sulfate]-glucosamine N-sulfotransferaseIUBMB Comments
The enzyme also catalyses the sulfation of chondroitin 4-sulfate and dermatan sulfate, but to a much more limited extent.
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Word Map
- 2.8.2.8
- proteoglycans
-
n-sulfation
- glycosaminoglycans
-
n-deacetylation
-
sulfotransferases
- 5'-phosphosulfate
-
ndsts
- 3'-phosphoadenosine
-
2-o-sulfation
-
2-o-sulfotransferase
-
n-unsubstituted
-
sulfuryl
- 3'-phosphoadenosine-5'-phosphosulfate
- hs3st1
- medicine
- diagnostics
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ndst1, ndst-1, ndst2, ndst3, ndst-2, ndst4, n-deacetylase/n-sulfotransferase-1, n-deacetylase/n-sulfotransferase, n-deacetylase/n-sulfotransferase 1, glucosaminyl n-deacetylase/n-sulfotransferase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3'-phosphoadenylyl-sulfate:heparitin N-sulfotransferase
-
-
-
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3'-phosphoadenylylsulfate:N-desulfoheparin N-sulfotransferase
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-
-
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3'-phosphoadenylylsulfate:N-desulfoheparin sulfotransferase
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-
-
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desulfoheparin sulfotransferase
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-
-
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heparan sulfate 2-N-sulfotransferase
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-
-
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heparan sulfate N-deacetylase/N-sulfotransferase
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-
-
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heparan sulfate N-sulfotransferase
-
-
-
-
heparan sulfate sulfotransferase
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-
-
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heparan sulfate/heparin N-deacetylase/N-sulfotransferase-1
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bifunctional enzyme
heparin N-sulfotransferase
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-
-
-
heparitin sulfotransferase
-
-
-
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N-desulfoheparin sulfotransferase
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-
-
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N-heparan sulfate sulfotransferase
-
-
-
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N-HSST
-
-
-
-
NDST-1
PAPS:DSH sulfotransferase
-
-
-
-
PAPS:N-desulfoheparin sulfotransferase
-
-
-
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sulfotransferase, desulfoheparin
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
sulfate group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine N-sulfotransferase
The enzyme also catalyses the sulfation of chondroitin 4-sulfate and dermatan sulfate, but to a much more limited extent.
CAS REGISTRY NUMBER
COMMENTARY
9026-75-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + (heparan sulfate)-glucosamine
adenosine 3',5'-bisphosphate + (heparan sulfate)-N-sulfoglucosamine
NDST-1 plays a role in lung development and repair following injury due to its influence on the activity of fibroblast growth factor
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
-
-
?
3'-phosphoadenylyl sulfate + N-desulfated heparin
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-D-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfo-D-glucosamine
-
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
3'-phosphoadenylylsulfate + de-N-sulfated heparin
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + desulfated N-acetylated heparan sulfate
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + heparan sulfate
adenosine 3',5'-bisphosphate + N-sulfoheparan sulfate
-
-
-
-
?
3'-phosphoadenylylsulfate + heparin
adenosine 3',5'-bisphosphate + N-sulfoheparin
-
-
-
-
?
3'-phosphoadenylylsulfate + N-acetyl heparosan
adenosine 3',5'-bisphosphate + ?
-
-
-
-
?
3'-phosphoadenylylsulfate + N-desulfated, N-acetylated heparin
adenosine 3',5'-bisphosphate + ?
-
poor substrate
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
substrate needs to be deacetylated
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
N-sulfated residues of heparan sulfate participate in the binding of this polymer to proteins as basic fibroblast growth factor
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
the sulfation of the nitrogen of glucosamine in heparan sulfate is an obligatory step for subsequent epimerization of D-glucuronic to L-iduronic acid and of O-sulfation of the sugar chains
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
important role of the enzyme in heparan sulfate assembly in glomerula basement membrane and glomerular epithelial cells and in the pathogenesis of proteinuria in puromycin aminonucleoside nephrosis
-
-
?
additional information
?
-
-
no acceptors: tyrosine-containing tripeptides, N-acetylated heparan sulfate, N-acetylated heparin, chondroitin
-
-
?
additional information
?
-
-
key enzyme in hepatic heparan sulfate biosynthesis.Diabetes substantially suppresses hepatic N-deacetylase/N-sulfotransferase-1 mRNA, protein and enzymatic activity. Suppression of hepatic N-deacetylase/N-sulfotransferase-1 may contribute to diabetic dyslipidemia. Stimulation of N-deacetylase/N-sulfotransferase-1 activity by AngII inhibitors may provide cardiovascular protection
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + (heparan sulfate)-glucosamine
adenosine 3',5'-bisphosphate + (heparan sulfate)-N-sulfoglucosamine
NDST-1 plays a role in lung development and repair following injury due to its influence on the activity of fibroblast growth factor
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-D-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfo-D-glucosamine
-
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
additional information
?
-
-
key enzyme in hepatic heparan sulfate biosynthesis.Diabetes substantially suppresses hepatic N-deacetylase/N-sulfotransferase-1 mRNA, protein and enzymatic activity. Suppression of hepatic N-deacetylase/N-sulfotransferase-1 may contribute to diabetic dyslipidemia. Stimulation of N-deacetylase/N-sulfotransferase-1 activity by AngII inhibitors may provide cardiovascular protection
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
N-sulfated residues of heparan sulfate participate in the binding of this polymer to proteins as basic fibroblast growth factor
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
-
the sulfation of the nitrogen of glucosamine in heparan sulfate is an obligatory step for subsequent epimerization of D-glucuronic to L-iduronic acid and of O-sulfation of the sugar chains
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.7 - 8.1
-
pH 5.7: 33% of activity maximum, pH 8.1: 54% of activity maximum
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
diabetes substantially suppresses hepatic N-deacetylase/N-sulfotransferase-1 mRNA, protein and enzymatic activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NDST1_RAT
882
1
100785
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of the bifunctional enzyme heparan sulfate/heparin N-deacetylase/N-sulfotransferase-1 in Saccharomyces cerevisiae, yeast-expressed enzyme has both N-deacetylase and N-sulfotransferase activities
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
diabetes substantially suppresses hepatic N-deacetylase/N-sulfotransferase-1 mRNA, protein and enzymatic activity. Suppression of hepatic N-deacetylase/N-sulfotransferase-1 may contribute to diabetic dyslipidemia. Stimulation of N-deacetylase/N-sulfotransferase-1 activity by AngII inhibitors may provide cardiovascular protection
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wei, Z.; Swiedler, S.J.; Ishihara, M.; Orellana, A.; Hirschberg, C.B.
A single protein catalyzes both N-deacetylation and N-sulfation during the biosynthesis of heparan sulfate
Proc. Natl. Acad. Sci. USA
90
3885-3888
1993
Rattus norvegicus
Hashimoto, Y.; Orellana, A.; Gil, G.; Hirschberg, C.B.
Molecular cloning and expression of rat liver N-heparan sulfate sulfotransferase
J. Biol. Chem.
267
15744-15750
1992
Rattus norvegicus
Mandon, E.; Kempner, E.S.; Ishihara, M.; Hirschberg, C.B.
A monomeric protein in the Golgi membrane catalyzes both N-deacetylation and N-sulfation of heparan sulfate
J. Biol. Chem.
269
11729-11733
1994
Rattus norvegicus
Brandan, E.; Hirschberg, C.B.
Purification of rat liver N-heparan-sulfate sulfotransferase
J. Biol. Chem.
263
2417-2422
1988
Rattus norvegicus
Kusche-Gullberg, M.; Eriksson, I.; Pikas, D.S.; Kjellen, L.
Identification and expression in mouse of two heparan sulfate glucosaminyl N-deacetylase/N-sulfotransferase genes
J. Biol. Chem.
273
11902-11907
1998
Rattus norvegicus, Mus musculus (Q3UHN9), Mus musculus
Williams, K.J.; Liu, M.L.; Zhu, Y.; Xu, X.; Davidson, W.R.; McCue, P.; Sharma, K.
Loss of heparan N-sulfotransferase in diabetic liver: role of angiotensin II
Diabetes
54
1116-1122
2005
Rattus norvegicus
Saribas, A.S.; Mobasseri, A.; Pristatsky, P.; Chen, X.; Barthelson, R.; Hakes, D.; Wang, J.
Production of N-sulfated polysaccharides using yeast-expressed N-deacetylase/N-sulfotransferase-1 (NDST-1)
Glycobiology
14
1217-1228
2004
Rattus norvegicus
Nakayama, K.; Natori, Y.; Sato, T.; Kimura, T.; Sugiura, A.; Sato, H.; Saito, T.; Ito, S.
Altered expression of NDST-1 messenger RNA in puromycin aminonucleoside nephrosis
J. Lab. Clin. Med.
143
106-114
2004
Rattus norvegicus
Newman, D.R.; Walsh, E.; Apparao, K.B.; Sannes, P.L.
Fibroblast growth factor-binding protein and N-deacetylase/N-sulfotransferase-1 expression in type II cells is modulated by heparin and extracellular matrix
Am. J. Physiol. Lung Cell Mol. Physiol.
293
1314-1320
2007
Rattus norvegicus (Q02353)
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