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Synonyms
3-o-sulfotransferase, 3-ost-1, hs3st1, 3-ost-4, 3-o-sulfotransferase-1, heparan sulfate d-glucosaminyl 3-o-sulfotransferase, hs3st5, heparan sulfate 3-o-sulfotransferase-1, 3-o-sulfotransferase isoform 1, 3-ost1,
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3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine
heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine
heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
3-phosphoadenylylsulfate + GlcNSO3(6-OSO3)-GlcA-GlcNSO3(6-OSO3)-IdoA(2-OSO3)-GlcNSO3(6-OSO3)
adenosine 3',5'-bisphosphate + GlcNSO3(6-OSO3)-GlcA-GlcNSO3(3,6-bisOSO3)-IdoA(2-OSO3)-GlcNSO3(6-OSO3)
3-phosphoadenylylsulfate + heparan sulfate
adenosine 3',5'-bisphosphate + ?
3-phosphoadenylylsulfate + heparin-glucosamine
?
3-phosphoadenylylsulfate + heparin-glucosamine
adenosine 3',5'-bisphosphate + heparin-glucosamine 3-O-sulfate
additional information
?
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3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine
heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
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?
3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine
heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
regulation of blood coagulation
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?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
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3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
3-OST catalyzes a key substitution in heparan sulfate sequences of biological importance, in particular heparan sulfate anticoagulant activity
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?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
residues R72, R67, K68, K123, and R276 are basic arginine/lysine residues located in a large open cleft of 3-OST-1 that is responsible for heparan sulfate binding
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3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine
heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
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-
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?
3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine
heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
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regulation of blood coagulation
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-
?
3-phosphoadenylylsulfate + GlcNSO3(6-OSO3)-GlcA-GlcNSO3(6-OSO3)-IdoA(2-OSO3)-GlcNSO3(6-OSO3)
adenosine 3',5'-bisphosphate + GlcNSO3(6-OSO3)-GlcA-GlcNSO3(3,6-bisOSO3)-IdoA(2-OSO3)-GlcNSO3(6-OSO3)
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?
3-phosphoadenylylsulfate + GlcNSO3(6-OSO3)-GlcA-GlcNSO3(6-OSO3)-IdoA(2-OSO3)-GlcNSO3(6-OSO3)
adenosine 3',5'-bisphosphate + GlcNSO3(6-OSO3)-GlcA-GlcNSO3(3,6-bisOSO3)-IdoA(2-OSO3)-GlcNSO3(6-OSO3)
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synthetic polysaccharide, O-sulfation almost exclusively in the 6-O-position of glucosamiyl residues
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?
3-phosphoadenylylsulfate + heparan sulfate
adenosine 3',5'-bisphosphate + ?
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?
3-phosphoadenylylsulfate + heparan sulfate
adenosine 3',5'-bisphosphate + ?
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converts inactive heparan sulfate in the precursor pool into active heparan sulfate
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?
3-phosphoadenylylsulfate + heparan sulfate
adenosine 3',5'-bisphosphate + ?
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enzyme generates sequences containing 6-sulfo-GlcUA-GlcN and 6-sulfo-GlcUA-GlcN
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?
3-phosphoadenylylsulfate + heparin-glucosamine
?
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enzyme brings about the final stage in biosynthesis of heparin
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?
3-phosphoadenylylsulfate + heparin-glucosamine
?
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biosynthesis of heparin/heparan sulfate
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?
3-phosphoadenylylsulfate + heparin-glucosamine
adenosine 3',5'-bisphosphate + heparin-glucosamine 3-O-sulfate
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heparin-related saccharides
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?
3-phosphoadenylylsulfate + heparin-glucosamine
adenosine 3',5'-bisphosphate + heparin-glucosamine 3-O-sulfate
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3-O-sulfation occurs only after the introduction of all other structural components required for the high affinity interaction with antithrombin, including the 6-O-sulfate groups on glucosamine unit 6
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?
3-phosphoadenylylsulfate + heparin-glucosamine
adenosine 3',5'-bisphosphate + heparin-glucosamine 3-O-sulfate
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only heparin with high affinity for antithrombin, i.e. HA heparin, acts as substrate
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?
additional information
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isoform 3OST-1 preferentially sulfates a GlcNS(+/-)6S with a D-glucuronic acid residue on its nonreducing end
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?
additional information
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combinatorial virtual library screening analysis of antithrombin binding oligosaccharide motif generation by heparan sulfate 3-O-sulfotransferase 1 (3OST-1). The enzyme prefers very well defined pentasaccharide sequences carrying distinct groups in each of the five residues to generate the antithrombin binding motif. Key residues include His271, Arg72, Arg197 and Lys173, which interact with 6-sulfate, 5-COO-, 2-/6-sulfates and 2-sulfate at the -2, -1, +2, and +1 positions of the precursor pentasaccharide, respectively. Additionally, uncharged residues, especially Gln163 and Asn167, are also identified as playing important roles in recognition, mechanism, overview
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additional information
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HS3ST-2 is a marker for specific subsets of TrkC-expressing cutaneous low-threshold mechanosensory and proprioceptive mechanosensory neurons, overview. HS3ST-2 is involved in formation of sensory endings of nerves in skeletal muscle
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3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine
heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
regulation of blood coagulation
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
3'-phosphoadenosine 5'-phosphosulfate + heparan sulfate-glucosamine
heparan sulfate 3-O-sulfo glucosamine + adenosine 3',5'-bisphosphate
-
regulation of blood coagulation
-
-
?
3-phosphoadenylylsulfate + heparin-glucosamine
?
additional information
?
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HS3ST-2 is a marker for specific subsets of TrkC-expressing cutaneous low-threshold mechanosensory and proprioceptive mechanosensory neurons, overview. HS3ST-2 is involved in formation of sensory endings of nerves in skeletal muscle
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-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
3-OST catalyzes a key substitution in heparan sulfate sequences of biological importance, in particular heparan sulfate anticoagulant activity
-
-
?
3-phosphoadenylylsulfate + heparin-glucosamine
?
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enzyme brings about the final stage in biosynthesis of heparin
-
-
?
3-phosphoadenylylsulfate + heparin-glucosamine
?
-
biosynthesis of heparin/heparan sulfate
-
-
?
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E90Q
site-directed mutagenesis, almost inactive mutant
H168A
the mutant shows 51.1% activity compared to the wild type enzyme
H168E
the mutant shows 19.1% activity compared to the wild type enzyme
K171A
the mutant shows 83.9% activity compared to the wild type enzyme
K284STP
truncated b3-OST-1 mutant, lacks C-terminal region from residue 284 to 307, exhibits a 200fold reduction in sulfotransferase activity
N167A
the mutant shows 40.4% activity compared to the wild type enzyme
R268A
the mutant shows 7.2% activity compared to the wild type enzyme
R276A
site-directed mutagenesis, almost inactive mutant
V164E
the mutant shows 20.3% activity compared to the wild type enzyme
D95A
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0.0% of the wild type activity
D95N
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8.1% of the wild type activity
E76A
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30.7% of the wild type activity
E76Q
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15.6% of the wild type activity
E88A
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63.7% of the wild type activity
E90A
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0.0% of the wild type activity
E90Q
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0.0% of the wild type activity
E98A
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65.6% of the wild type activity
H271A
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109.0% of the wild type activity
H271F
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42.0% of the wild type activity
H92A
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0.0% of the wild type activity
H92F
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0.0% of the wild type activity
K123A
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0.2% of the wild type activity
K132A
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49.1% of the wild type activity
K274A
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17.4% of the wild type activity
K68A
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0.1% of the wild type activity
N89A
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69.5% of the wild type activity
N89D
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74.4% of the wild type activity
Q163A
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34.0% of the wild type activity
R197A
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95.0% of the wild type activity
R276A
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0.3% of the wild type activity
R67A
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1.2% of the wild type activity
R67E
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1.1% of the wild type activity
R72A
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7.0% of the wild type activity
R72E
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0.0% of the wild type activity
S273A
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99.6% of the wild type activity
W96A
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91.6% of the wild type activity
W96F
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69.1% of the wild type activity
additional information
mutation of residues R72, R67, K68, and K123, lead to inactive mutant enzymes
additional information
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generation of a HS3ST-2-hPLAP knock-in mouse line, in which HS3ST-2-expressing neurons and their projections are labeled by human placental alkaline phosphatase, hPLAP, analysis fo the morphological subtypes of HS3ST-2/TrkC-expressing neurons, phenotype: in the glabrous and hairy skin, rare Merkel endings and transverse lanceolate endings are weakly stained, subcellular localization of the isozyme HS3ST-2, overview
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Kusche, M.; Bckstrm, G.; Riesenfeld, J.; Petitou, M.; Choay, J.; Lindahl, U.
Biosynthesis of heparin. O-sulfation of the antithrombin-binding region
J. Biol. Chem.
263
15474-15484
1988
Mus musculus
brenda
Kusche, M.; Torri, G.; Casu, B.; Lindahl, U.
Biosynthesis of heparin. Availability of glucosaminyl 3-O-sulfation sites
J. Biol. Chem.
265
7292-7300
1990
Mus musculus
brenda
Razi, N.; Lindahl, U.
Biosynthesis of heparin/heparan sulfate. The D-glucosaminyl 3-O-sulfotransferase reaction: target and inhibitor saccharides
J. Biol. Chem.
270
11267-11275
1995
Mus musculus
brenda
Zhang, L.; Lawrence, R.; Schwartz, J.J.; Bai, X.; Wei, G.; Esko, J.D.; Rosenberg, R.D.
The effect of precursor structures on the action of glucosaminyl 3-O-sulfotransferase-1 and the biosynthesis of anticoagulant heparan sulfate
J. Biol. Chem.
276
28806-28813
2001
Mus musculus
brenda
Zhang, L.; Schwartz, J.J.; Miller, J.; Liu, J.; Fritze, L.M.; Shworak, N.W.; Rosenberg, R.D.
The retinoic acid and cAMP-dependent up-regulation of 3-O-sulfotransferase-1 leads to a dramatic augmentation of anticoagulantly active heparan sulfate biosynthesis in F9 embryonal carcinoma cells
J. Biol. Chem.
273
27998-28003
1998
Mus musculus
brenda
Edavettal, S.C.; Carrick, K.; Shah, R.R.; Pedersen, L.C.; Tropsha, A.; Pope, R.M.; Liu, J.
A conformational change in heparan sulfate 3-O-sulfotransferase-1 is induced by binding to heparan sulfate
Biochemistry
43
4680-4688
2004
Mus musculus (O35310)
brenda
Edavettal, S.C.; Lee, K.A.; Negishi, M.; Linhardt, R.J.; Liu, J.; Pedersen, L.C.
Crystal structure and mutational analysis of heparan sulfate 3-O-sulfotransferase isoform 1
J. Biol. Chem.
279
25789-25797
2004
Mus musculus
brenda
Munoz, E.; Xu, D.; Kemp, M.; Zhang, F.; Liu, J.; Linhardt, R.J.
Affinity, kinetic, and structural study of the interaction of 3-O-sulfotransferase isoform 1 with heparan sulfate
Biochemistry
45
5122-5128
2006
Mus musculus (O35310)
brenda
Hasegawa, H.; Wang, F.
Visualizing mechanosensory endings of TrkC-expressing neurons in HS3ST-2-hPLAP mice
J. Comp. Neurol.
511
543-556
2008
Mus musculus
brenda
Liu, J.; Moon, A.; Sheng, J.; Pedersen, L.
Understanding the substrate specificity of the heparan sulfate sulfotransferases by an integrated biosynthetic and crystallographic approach
Curr. Opin. Struct. Biol.
22
550-557
2012
Mus musculus (O35310)
brenda
Moon, A.F.; Xu, Y.; Woody, S.M.; Krahn, J.M.; Linhardt, R.J.; Liu, J.; Pedersen, L.C.
Dissecting the substrate recognition of 3-O-sulfotransferase for the biosynthesis of anticoagulant heparin
Proc. Natl. Acad. Sci. USA
109
5265-5270
2012
Mus musculus (O35310)
brenda
Datta, P.; Li, G.; Yang, B.; Zhao, X.; Baik, J.Y.; Gemmill, T.R.; Sharfstein, S.T.; Linhardt, R.J.
Bioengineered Chinese hamster ovary cells with Golgi-targeted 3-O-sulfotransferase-1 biosynthesize heparan sulfate with an antithrombin-binding site
J. Biol. Chem.
288
37308-37318
2013
Mus musculus (O35310)
brenda
Thacker, B.E.; Xu, D.; Lawrence, R.; Esko, J.D.
Heparan sulfate 3-O-sulfation: a rare modification in search of a function
Matrix Biol.
35
60-72
2014
Mus musculus (O35310)
brenda
Sankaranarayanan, N.V.; Bi, Y.; Kuberan, B.; Desai, U.R.
Combinatorial virtual library screening analysis of antithrombin binding oligosaccharide motif generation by heparan sulfate 3-O-Sulfotransferase 1
Comput. Struct. Biotechnol. J.
18
933-941
2020
Mus musculus (O35310)
brenda