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Information on EC 2.8.2.21 - keratan sulfotransferase and Organism(s) Homo sapiens and UniProt Accession O43916
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2.8.2.21 keratan sulfotransferaseIUBMB Comments
Sulfation takes place at the 6-position of galactosyl and N-acetylglucosaminyl residues in keratan, a proteoglycan. Not identical with EC 2.8.2.5 (chondroitin 4-sulfotransferase), EC 2.8.2.6 (choline sulfotransferase) or EC 2.8.2.17 (chondroitin 6-sulfotransferase).
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Word Map
- 2.8.2.21
-
sulfotransferases
- l-selectin
- 5'-phosphosulfate
- n-acetyllactosamine
- 3'-phosphoadenosine
-
6-o-sulfation
- glycam-1
-
6-sulfated
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n-acetylglucosamine-6-o-sulfotransferase
-
retnlb
-
addressin
-
siglec-f
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
chst1, ksgal6st, chst5, glcnac 6-o-sulfotransferase, glcnac6st, keratan sulfate sulfotransferase, gn6st, keratan sulfate gal-6-sulfotransferase, keratan sulfate galactose 6-o-sulfotransferase, galactose-6-sulfotransferase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3'-phosphoadenylyl keratan sulfotransferase
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-
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3'-phosphoadenylylsulfate:keratan sulfotransferase
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Gal-6-sulfotransferase
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galactose-6-sulfotransferase
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GlcNAc 6-O-sulfotransferase
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GlcNAc 6-sulfotransferase
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GlcNAc sulfotransferase
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GlcNAc6ST
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-
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Gn6ST
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-
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GST
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-
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GST3
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GST4alpha
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-
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GST4beta
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-
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keratan sulfate Gal-6-sulfotransferase
keratan sulfate sulfotransferase
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KSG6ST
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KSGal6ST
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KSST
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LSST
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N-acetylgalactosamine 6-O-sulfotransferase
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N-acetylglucosaminyl sulfotransferase
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sulfotransferase, keratan
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keratan sulfate Gal-6-sulfotransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
sulfate group transfer
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-
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:keratan 6'-sulfotransferase
Sulfation takes place at the 6-position of galactosyl and N-acetylglucosaminyl residues in keratan, a proteoglycan. Not identical with EC 2.8.2.5 (chondroitin 4-sulfotransferase), EC 2.8.2.6 (choline sulfotransferase) or EC 2.8.2.17 (chondroitin 6-sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY
62168-79-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + keratan sulfate
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
sulfation at position 6 of Gal residues which are adjacent to GlcNAc(6S) or GlcNAc
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-
?
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
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-
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?
3'-phosphoadenylylsulfate + GlcNAcbeta1-3Galbeta1-4GlcNACbeta1-3Galbeta1-4GlcNAcbeta1-6Manalpha1-6Manbeta-octyl
adenosine 3',5'-bisphosphate + ?
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synthetic substrate, sulfation at position 6 of N-acetylgalactosamine residues, GlcNAc 6-O-sulfotransferase
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-
?
3'-phosphoadenylylsulfate + GlyCAM-1
adenosine 3',5'-bisphosphate + ?
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endothelial mucin-like ligand for L-selectin, products correspond to [SO3-6]GlcNAc and Galbeta1-4[SO3-6]GlcNAc
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-
?
3'-phosphoadenylylsulfate + keratan sulfate
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
3'-phosphoadenylylsulfate + NeuAcalpha2-3Galbeta1(6-sulfo)GlcNAcbeta1-3(6-sulfo)Galbeta1-4(6-sulfo)GlcNAc
adenosine 3',5'-bisphosphate + NeuAcalpha2-3-(6-sulfo)Galbeta1-(6-sulfo)GlcNAcbeta1-3(6-sulfo)Galbeta1-4(6-sulfo)GlcNAc
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sulfation at position 6 of Gal residues
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?
3'-phosphoadenylylsulfate + NeuAcalpha2-3Galbeta1-4GlcNAc
adenosine 3',5'-bisphosphate + NeuAcalpha2-3(6-sulfo)Galbeta1-4GlcNAc
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sulfation at position 6 of Gal residues, no sulfation of GlcNAc
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?
3'-phosphoadenylylsulfate + NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAc
adenosine 3',5'-bisphosphate + ?
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sulfation at position 6 of Gal residues
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-
?
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
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-
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?
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
keratan sulfate proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain
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-
?
3'-phosphoadenylylsulfate + keratan sulfate
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
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?
3'-phosphoadenylylsulfate + keratan sulfate
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
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sulfation at position 6 of N-acetylgalactosamine residues, GlcNAc 6-O-sulfotransferase
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?
additional information
?
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no activity towards chondroitin, chondroitin sulfate, dermatan sulfate or CDSNS-heparinn
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?
additional information
?
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no activity towards chondroitin, chondroitin sulfate, dermatan sulfate or CDSNS-heparinn
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?
additional information
?
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sulfation of GlcNAc residues precedes sulfation of Gal residues during biosynthesis of keratan sulfate
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?
additional information
?
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sulfation of GlcNAc residues precedes sulfation of Gal residues during biosynthesis of keratan sulfate
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?
additional information
?
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Gn6ST produces keratan sulfate in cooperation with KSG6ST, inactivation of Gn6ST may lead to macular corneal dystrophy
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?
additional information
?
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loss of GST-4beta causes macular corneal dystrophy
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
keratan sulfate proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain
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-
?
3'-phosphoadenylyl sulfate + keratan
adenosine 3',5'-bisphosphate + keratan 6'-sulfate
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-
-
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?
additional information
?
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sulfation of GlcNAc residues precedes sulfation of Gal residues during biosynthesis of keratan sulfate
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-
?
additional information
?
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sulfation of GlcNAc residues precedes sulfation of Gal residues during biosynthesis of keratan sulfate
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-
?
additional information
?
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Gn6ST produces keratan sulfate in cooperation with KSG6ST, inactivation of Gn6ST may lead to macular corneal dystrophy
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?
additional information
?
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loss of GST-4beta causes macular corneal dystrophy
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
glioblastoma cell line, highly expresses keratan sulfate on a cell surface and expresses a high level of keratan sulfate Gal-6-sulfotransferase
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intestinal isoform of GlcNAc6T, predominantly expressed in intestine
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
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KS6ST transfers sulfate to the C-6 of Gal of LacNAc on glycoproteins and is responsible for the generation of a Langerin ligands. Langerin plays roles in the first line defense against pathogens and in Birbeck granule formation in Langerhans cells, vLangerin is categorized as a C-type lectin. It recognizes pathogenic fungi, such as Candida sp. and Malassezia sp., expressing heavily mannosylated glycans, Langerin recognizes 6'-sulfo-LacNAc as a preferred, glycan ligand, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CHST1_HUMAN
411
1
46715
Swiss-Prot
Secretory Pathway (Reliability: 1)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
stable expression and co-expression of Langerin gene and keratan sulfate Gal-6-sulfotransferase gene CHST1 in CHO cells, expression of fluorescent tagged Langerin, quantitative real-time PCR analysis of the KS6ST in human tissue
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Akama, T.O.; Nakayama, J.; Nishida, K.; Hiraoka, N.; Suzuki, M.; McAuliffe, J.; Hindsgaul, O.; Fukuda, M.; Fukuda, M.N.
Human corneal GlcNac 6-O-sulfotransferase and mouse intestinal GlcNac 6-O-sulfotransferase both produce keratan sulfate
J. Biol. Chem.
276
16271-16278
2001
Homo sapiens, Mus musculus
Bartes, A.; Bhakta, S.; Hemmerich, S.
Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta)
Biochem. Biophys. Res. Commun.
282
928-933
2001
Homo sapiens
Fukuta, M.; Inazawa, J.; Torii, T.; Tsuzuki, K.; Shimada, E.; Habuchi, O.
Molecular cloning and characterization of human keratan sulfate Gal-6-sulfotransferase
J. Biol. Chem.
272
32321-32328
1997
Homo sapiens (O43916), Homo sapiens
Torii, T.; Fukuta, M.; Habuchi, O.
Sulfation of sialyl N-acetyllactosamine oligosaccharides and fetuin oligosaccharides by keratan sulfate Gal-6-sulfotransferase
Glycobiology
10
203-211
2000
Homo sapiens
Hayatsu, N.; Ogasawara, S.; Kaneko, M.K.; Kato, Y.; Narimatsu, H.
Expression of highly sulfated keratan sulfate synthesized in human glioblastoma cells
Biochem. Biophys. Res. Commun.
368
217-222
2008
Homo sapiens (O43916), Homo sapiens
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