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Information on EC 2.8.2.17 - chondroitin 6-sulfotransferase and Organism(s) Rattus norvegicus and UniProt Accession Q9QZL2

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EC Tree
     2 Transferases
         2.8 Transferring sulfur-containing groups
             2.8.2 Sulfotransferases
                2.8.2.17 chondroitin 6-sulfotransferase
IUBMB Comments
The sulfation is at the 6-position of N-acetylgalactosamine residues of chondroitin. Not identical with EC 2.8.2.5 chondroitin 4-sulfotransferase.
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q9QZL2
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
chst3, c6st, c6st-1, chondroitin 6-sulfotransferase, carbohydrate sulfotransferase 3, c6st1, chondroitin 6-o-sulfotransferase, chondroitin 6-o-sulfotransferase-1, chondroitin-6-sulfotransferase, chondroitin 6-sulfotransferase-1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
chondroitin 6-sulphotransferase-1
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3'-phosphoadenosine 5'-phosphosulfate:chondroitin sulfate sulfotransferase
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C6ST
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chondroitin 6-O-sulfotransferase
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CST
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-
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sulfotransferase, chondroitin 6-
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-
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terminal 6-sulfotransferase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:chondroitin 6'-sulfotransferase
The sulfation is at the 6-position of N-acetylgalactosamine residues of chondroitin. Not identical with EC 2.8.2.5 chondroitin 4-sulfotransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
37292-93-6
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83589-04-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
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-
-
?
additional information
?
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upregulation of chondroitin 6-sulphotransferase-1 facilitates Schwann cell migration during axonal growth, overview
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
additional information
?
-
upregulation of chondroitin 6-sulphotransferase-1 facilitates Schwann cell migration during axonal growth, overview
-
-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000002
recombinant truncated mutant enzyme in COS-7 cell extract
0.00000022
recombinant wild-type enzyme in COS-7 cell extract
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
perivascular, epineurial and perineurial
Manually annotated by BRENDA team
increased enzyme expression in mobile cells, downregulation accompanies declining mobility of Schwann cells as they engaged in the myelination of re-growing axons
Manually annotated by BRENDA team
additional information
the C6st1 expression is upregulated GFAP-positive Schwann cells in an early phase of tissue reorganisation after nerve crush, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHST3_RAT
474
1
54018
Swiss-Prot
other Location (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene C6ST, recombinant expression in Bacillus subtilis strain E168C, Escherichia coli strain BL21(DE3), and Pichia pastoris strain GS115, coexpression with ASSTIV and C4ST from Rattus norvegicus, subcloning in Escherichia coli strain JM109. Chondroitin extraction and quantification
gene C6st1, DNA and amino acid sequence determination and analysis, expression analysis and sequence comparison, expression of HIs-tagged full-length enzyme and truncated enzyme, lacking the N-terminal transmembrane domain but retaining the PAPS-binding sites and the catalytic domain, in COS-7 cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
the enzyme is used for large scale chondroitin sulfate synthesis in a coupled system with aryl sulfotransferase IV and chondroitin 4-sulfotransferase, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Liu, J.; Chau, C.; Liu, H.; Jang, B.R.; Li, X.; Chan, Y.; Shum, D.K.
Upregulation of chondroitin 6-sulphotransferase-1 facilitates Schwann cell migration during axonal growth
J. Cell Sci.
119
1196
2006
Rattus norvegicus (Q9QZL2)
Manually annotated by BRENDA team
Zhou, Z.; Li, Q.; Huang, H.; Wang, H.; Wang, Y.; Du, G.; Chen, J.; Kang, Z.
A microbial-enzymatic strategy for producing chondroitin sulfate glycosaminoglycans
Biotechnol. Bioeng.
115
1561-1570
2018
Rattus norvegicus (Q9QZL2)
Manually annotated by BRENDA team