Information on EC 2.8.2.17 - chondroitin 6-sulfotransferase

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The expected taxonomic range for this enzyme is: Chordata

EC NUMBER
COMMENTARY
2.8.2.17
-
RECOMMENDED NAME
GeneOntology No.
chondroitin 6-sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
chondroitin sulfate biosynthesis (late stages)
-
Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:chondroitin 6'-sulfotransferase
The sulfation is at the 6-position of N-acetylgalactosamine residues of chondroitin. Not identical with EC 2.8.2.5 chondroitin 4-sulfotransferase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3'-phosphoadenosine 5'-phosphosulfate:chondroitin sulfate sulfotransferase
-
-
-
-
C6ST
-
-
-
-
C6ST-1
O88199
-
C6ST-1
Q9QZL2
-
carbohydrate sulfotransferase 3
Q7LGC8
-
chondroitin 6-O-sulfotransferase
-
-
-
-
chondroitin 6-O-sulfotransferase
-
-
chondroitin 6-O-sulfotransferase-1
Q7LGC8
-
chondroitin 6-sulfotransferase-1
O88199
-
chondroitin 6-sulphotransferase-1
Q9QZL2
-
chondroitin-6-sulfotransferase
-
-
CHST3
Q7LGC8
-
CST
-
-
-
-
sulfotransferase, chondroitin 6-
-
-
-
-
terminal 6-sulfotransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37292-93-6
-
83589-04-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
calf
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
gene CHST3
SwissProt
Manually annotated by BRENDA team
patients with a distinct form of spondyloepiphyseal dysplasia, SED Omani type
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
recombinant enzyme with FLAG-tag
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
gene Chst3, C6st, or Gst0; isozyme C6ST-1
SwissProt
Manually annotated by BRENDA team
wild-type and knock-out mutant
-
-
Manually annotated by BRENDA team
carbohydrate sulfotransferase 3; gene C6st1
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
deficiency of CHST3 is associated with a phenotype of severe chondrodysplasia with progressive spinal involvement
malfunction
-
Omani-type spondyloepiphyseal dysplasia with cardiac involvement caused by a missense mutation in CHST3
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
Q7LGC8
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6-sulfate
show the reaction diagram
O88199
-
-
-
?
3'-phosphoadenylyl sulfate + DELTA4HexUAalpha(1->3)Galbeta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + DELTA4HexUAalpha(1->3)Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
24% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + DELTA4HexUAalpha(1->3)GalNAc(4S)beta(1->4)GlcUAbeta(1->3)Gal(4S)beta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + DELTA4HexUAalpha(1->3)GalNAc(4S)beta(1->4)GlcUAbeta(1->3)Gal(4S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
270% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + DELTA4HexUAalpha(1->3)GalNAc(4S)beta(1->4)GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + DELTA4HexUAalpha(1->3)GalNAc(4S)beta(1->4)GlcUAbeta(1->3)Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
260% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + DELTA4HexUAalpha(1->3)GalNAc(6S)beta(1->4)GlcUAbeta(1->3)Gal-Gal(6S)beta(1->4)Xyl-ol
adenosine 3',5'-bisphosphate + DELTA4HexUAalpha(1->3)GalNAc(6S)beta(1->4)GlcUAbeta(1->3)Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xyl-ol
show the reaction diagram
-
360% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + DELTA4HexUAalpha(1->3)GalNAc(6S)beta(1->4)GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl(2P)-ol
adenosine 3',5'-bisphosphate + DELTA4HexUAalpha(1->3)GalNAc(6S)beta(1->4)GlcUAbeta(1->3)Gal(6S)beta(1->3)-Galbeta(1->4)Xyl(2P)-ol
show the reaction diagram
-
290% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + DELTA4HexUAalpha(1->4)GlcNAc(6S)-GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + DELTA4HexUAalpha(1->4)GlcNAc(6S)beta(1->3)GlcUAbeta(1->3)Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
17% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + Galbeta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
4% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + GalNAcbeta(1->4)GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + GalNAc(6S)beta(1->4)GlcUAbeta(1->3)Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
84% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + GlcNAcalpha(1->4)GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + GlcNAcalpha(1->4)GlcUAbeta(1->3)Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
2% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + GlcUAbeta(1->3)Gal(4S)beta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + GlcUAbeta(1->3)Gal(4S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
20% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
adenosine 3',5'-bisphosphate + GlcUAbeta(1->3)Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + GlcUAbeta(1->3)Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
8% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + GlcUAbeta(1->3)GalNAc(4S)beta(1->4)GlcUAbeta(1->3)Gal(4S)beta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + GlcUAbeta(1->3)GalNAc(4S)beta(1->4)GlcUAbeta(1->3)Gal(4S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
250% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + GlcUAbeta(1->3)GalNAcbeta(1->4)GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
adenosine 3',5'-bisphosphate + GlcUAbeta(1->3)GalNAc(6S)beta(1->3)GlcUAbeta(1->3)Gal(6S)beta(1->3)Gal(6S)beta(1->4)Xylbeta(1->O)-Ser
show the reaction diagram
-
130% of the activity with GlcUAbeta(1->3)Galbeta(1->3)Galbeta(1->4)Xyl-(Gly)Ser-(Gly-Glu)
-
-
?
3'-phosphoadenylyl sulfate + keratan sulfate
?
show the reaction diagram
O88199
-
-
-
?
3'-phosphoadenylylsulfate + 1-phospho-N-acetylgalactosamine 4-sulfate
adenosine 3',5'-bisphosphate + 1-phospho-N-acetylgalactosamine 4,6-bissulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + beta-benzyl N-acetylglucosamine
adenosine 3',5'-bisphosphate + beta-benzyl N-acetylglucosaminesulfate
show the reaction diagram
-
enzyme does not use alpha-or beta-benzyl N-acetylgalactosamine
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-, O88199
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
Q92179
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
Q9NS84
incorporates sulfate exclusively into GalNAc C-6 position
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
incorporates sulfate exclusively into GalNAc C-6 position
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
specificity towards desulfated chondroitin sulfate and oligosaccharides derived therefrom
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
native chondroitin is the best acceptor, chemically desulfated chondroitin sulfate has the lowest rate of sulfation
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
involved in biosynthesis of chondroitin sulfate
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
is believed to act in the course of chondroitin sulfate synthesis in cooperation with, but shortly after the enzymes involved in the chain elongation reaction
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
involved in the maintenance of native T lymphocytes in the spleen
-
?
3'-phosphoadenylylsulfate + chondroitin 4-sulfate
adenosine 3',5'-bisphosphate + chondroitin 4,6-bissulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin 4-sulfate
adenosine 3',5'-bisphosphate + chondroitin 4,6-bissulfate
show the reaction diagram
-
incorporates sulfate into position 6 of the nonreducing N-acetylgalactosamine 4-sulfate end group of chondroitin, no activity with unsulfated chondroitin
-
?
3'-phosphoadenylylsulfate + chondroitin 4-sulfate
adenosine 3',5'-bisphosphate + chondroitin 4,6-bissulfate
show the reaction diagram
-
may play a role in synthesis of proteoglycans
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate A
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
6.2% of the activity with chondroitin
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate C
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
3% of the activity with chondroitin
-
-
?
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides
?
show the reaction diagram
-
even-numbered oligosaccharides with a glucuronic acid at the nonreducing terminus are active as acceptors, their capacity decreasing with decreasing chain length: dodecasaccharide at 64%, decasaccharide at 55%, octasaccharide at 17%, hexasaccharide at 4% of the chondroitin sulfation
-
-
?
3'-phosphoadenylylsulfate + corneal keratan sulfate
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + corneal keratan sulfate
?
show the reaction diagram
-, O88199
-
-
-
?
3'-phosphoadenylylsulfate + corneal keratan sulfate
?
show the reaction diagram
Q92179
-
-
-
?
3'-phosphoadenylylsulfate + corneal keratan sulfate
?
show the reaction diagram
-
-
sulfated product is degraded by keratanase but not by chondroitinase ABC
?
3'-phosphoadenylylsulfate + corneal keratan sulfate
?
show the reaction diagram
Q7LGC8
transfers sulfate to position 6 of Gal residues
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
95% of the activity with chondroitin
-
-
?
3'-phosphoadenylylsulfate + N-acetylgalactosamine 4-sulfate
adenosine 3',5'-bisphosphate + N-acetylgalactosamine 4,6-bissulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + phenyl 2-acetamido-2-deoxy-4-O-sulfonato-D-galactopyranose
adenosine 3',5'-bisphosphate + phenyl 2-acetamido-2-deoxy-4,6-di-O-sulfonato-D-galactopyranose
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + UDP-N-acetylgalactosamine 4-sulfate
adenosine 3',5'-bisphosphate + UDP-N-acetylgalactosamine 4,6-bissulfate
show the reaction diagram
-
-
-
?
additional information
?
-
-
overview: sulfation of chondroitin sulfates
-
-
-
additional information
?
-
-
no substrate: GDPmannose
-
-
-
additional information
?
-
-
no substrate: hyaluronate
-
-
-
additional information
?
-
-
no substrate: UDP-N-acetylglucosamine 6-sulfate, no substrate: UDPgalactose, no substrate: UDP-N-acetylgalactosamine
-
-
-
additional information
?
-
-
no substrate: heparin
-
-
-
additional information
?
-
-
no substrate: heparin, no substrate: heparan sulfate
-
-
-
additional information
?
-
Q9NS84
keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc sequence hardly serve as acceptors for C6ST-2
-
-
-
additional information
?
-
-
overview: sulfation of sialyl lactosamine oligosaccharides
-
-
-
additional information
?
-
-
dermatan sulfate and keratan sulfate preparations which contain covalently bound chondroitin sulfate act as sulfate acceptors
-
-
-
additional information
?
-
-
no substrate: UDP-N-acetylglucosamine
-
-
-
additional information
?
-
-
does not sulfate a non-reducing terminal GalNAc residue when this residue is added concurrently with the sulfation
-
-
-
additional information
?
-
-
no substrate: UDP-N-acetylgalactosamine 6-sulfate
-
-
-
additional information
?
-
-
no substrate: dermatan sulfate. Enzyme mainly transfers sulfate to GalNAc residues located in the L-iduronic acid rich region with little preference for the GalNAc residue neighbouring the GlcA residue
-
-
-
additional information
?
-
Q9QZL2
upregulation of chondroitin 6-sulphotransferase-1 facilitates Schwann cell migration during axonal growth, overview
-
-
-
additional information
?
-
-
the enzyme mediates the sulfation of proteoglycans, particularly chondroitin sulfate, in the extracellular matrix of cartilage
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
Q7LGC8
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6-sulfate
show the reaction diagram
O88199
-
-
-
?
3'-phosphoadenylyl sulfate + keratan sulfate
?
show the reaction diagram
O88199
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
involved in biosynthesis of chondroitin sulfate
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
is believed to act in the course of chondroitin sulfate synthesis in cooperation with, but shortly after the enzymes involved in the chain elongation reaction
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
involved in the maintenance of native T lymphocytes in the spleen
-
?
3'-phosphoadenylylsulfate + chondroitin 4-sulfate
adenosine 3',5'-bisphosphate + chondroitin 4,6-bissulfate
show the reaction diagram
-
may play a role in synthesis of proteoglycans
-
-
?
additional information
?
-
Q9QZL2
upregulation of chondroitin 6-sulphotransferase-1 facilitates Schwann cell migration during axonal growth, overview
-
-
-
additional information
?
-
-
the enzyme mediates the sulfation of proteoglycans, particularly chondroitin sulfate, in the extracellular matrix of cartilage
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ba2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Ca2+
-
divalent cation required, Mn2+, Mg2+, Ca2+
Ca2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Co2+
-
good activator, stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Cu2+
-
can partially replace Mn2+ in activation
Cu2+
-
stimulates chondroitin 6-sulfotransferase activity but not keratan sulfate sulfotransferase activity
Mg2+
-
can partially replace Mn2+ in activation
Mg2+
-
divalent cation required, Mn2+, Mg2+, Ca2+
Mg2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Mn2+
-
stimulates
Mn2+
-
best activator
Mn2+
-
divalent cation required, Mn2+, Mg2+, Ca2+
Sr2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Zn2+
-
can partially replace Mn2+ in activation
Mn2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
additional information
-
Ca2+ has no effect
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4-chloromercuribenzoate
-
1 mM
ADP
-
60% inhibition at 0.12 mM
CaCl2
-
90% inhibition at 5 mM
CoCl2
-
50% inhibition at 5 mM
EDTA
-
in absence of added metal ion
heparin
-
40% inhibition in the nanomolar range
MgCl2
-
40% inhibition at 5 mM
phenyl 2-acetamido-2-deoxy-4-O-sulfonato-D-galactopyranose
-
competitive, 5 mM, 76% inhibition, specific
-
PNGase F
O88199
deglycosylation inactivates the enzyme
-
MnCl2
-
75% inhibition at 5 mM
additional information
-
no inhibition by detergents of enzyme in chicken cartilage system
-
additional information
-
not: iodoacetamide
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Basic proteins
-
stimulate
-
Chondroitin sulfates
-
competitive inhibitors
Histone
-
stimulates, optimum concentration: 0.5 mg/ml
Lysozyme
-
stimulates, optimum concentration: 3.0 mg/ml
-
poly-L-lysine
-
stimulates 2fold at 1.5 mg/ml
Polyamines
-
stimulate
-
protamine
-
stimulates, optimum concentration: 0.025 mg/ml
protamine
-
stimulates 2fold at 1.5 mg/ml
protamine
-
12fold activation of enzyme activity with totally desulfated chondroitin, 3fold activation with partially sulfated chondroitin
protamine
-
-
spermidine
-
stimulates, optimum concentration: 2.5 mM
spermidine
-
stimulates 2fold at 1 mM
spermine
-
stimulates, optimum concentration: 0.6 mM
spermine
-
stimulates 2fold at 1 mM
Lysozyme
-
stimulates 2fold at 1.5 mg/ml
-
additional information
-
enzyme is present in supernatant and in particulate fraction, detergent activates indirectly by releasing enzyme into the medium
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00034
-
3'-phosphoadenylyl sulfate
O88199
pH 6.8, 37C, recombinant mutant N250S, with chondroitin
0.0004
-
3'-phosphoadenylyl sulfate
O88199
pH 6.8, 37C, recombinant mutant N457S, with chondroitin
0.0011
-
3'-phosphoadenylyl sulfate
O88199
pH 6.8, 37C, recombinant wild-type enzyme, with chondroitin
0.0029
-
3'-phosphoadenylyl sulfate
O88199
pH 6.8, 37C, recombinant mutant N413S, with keratan sulfate
0.0038
-
3'-phosphoadenylyl sulfate
O88199
pH 6.8, 37C, recombinant wild-type enzyme, with keratan sulfate
0.00417
-
3'-phosphoadenylyl sulfate
O88199
pH 6.8, 37C, recombinant mutant N413S, with chondroitin
0.00011
-
3'-phosphoadenylylsulfate
-
+ UDP-N-acetylgalactosamine 4-sulfate, pH 7, 37C
0.0007
-
3'-phosphoadenylylsulfate
-
pH 6.8, 37C
0.0091
-
3'-phosphoadenylylsulfate
-
in the presence of protamine, pH 8, 37C
0.018
-
3'-phosphoadenylylsulfate
-
pH 8.2, 37C
0.0333
-
3'-phosphoadenylylsulfate
-
in the absence of protamine, pH 8, 37C
0.00076
-
Chondroitin
O88199
pH 6.8, 37C, recombinant mutant N250S
0.00103
-
Chondroitin
O88199
pH 6.8, 37C, recombinant mutant N457S
0.00144
-
Chondroitin
O88199
pH 6.8, 37C, recombinant mutant N413S
0.00146
-
Chondroitin
O88199
pH 6.8, 37C, recombinant wild-type enzyme
0.15
-
Chondroitin
-
1-phospho-N-acetylgalactosamine 4-sulfate, pH 7, 37C
0.15
-
Chondroitin
-
-
0.00049
-
chondroitin 4-sulfate
-
pH 7, 37C
0.038
-
chondroitin 4-sulfate
-
pH 6.8, 37C
0.9
-
decasaccharide
-
derived from chondroitin
-
1.8
-
Desulfated chondroitin sulfate
-
pH 8.2, 37C
0.48
-
dodecasaccharide
-
derived from chondroitin
-
0.05
-
GlcUAbeta(1->3)GalNAc(4S)beta(1->4)GlcUAbeta(1->3)Gal(4S)beta(1->3)Galbeta(1->4)Xylbeta(1->O)-Ser
-
pH 6.8, 37C
5
-
hexasaccharide
-
derived from chondroitin
-
0.0029
-
Keratan sulfate
O88199
pH 6.8, 37C, recombinant mutant N413S
0.36
-
N-acetylgalactosamine 4-sulfate
-
pH 7, 37C
0.06
-
native chondroitin
-
pH 8.2, 37C
-
2.5
-
Octasaccharide
-
derived from chondroitin
-
0.027
-
UDP-N-acetylgalactosamine 4-sulfate
-
pH 7, 37C
0.005
-
Keratan sulfate
O88199
pH 6.8, 37C, recombinant wild-type enzyme
additional information
-
additional information
-
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.98
-
phenyl 2-acetamido-2-deoxy-4-O-sulfonato-D-galactopyranose
-
pH 6.8, 37C
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0000002
-
Q9QZL2
recombinant truncated mutant enzyme in COS-7 cell extract
0.00000022
-
Q9QZL2
recombinant wild-type enzyme in COS-7 cell extract
0.0000005
0.0000011
-
wild-type activity in fibroblasts
0.000231
-
-, O88199
recombinant protein expressed in COS-7 cells
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.4
-
-
-
5.5
8.5
-
-
5.8
-
-
chondroitin 6-sulfotransferase activity
6
-
-
double pH-optimum: 100% at pH 6.0 and 65% at pH 7.3
6.2
-
-
keratan sulfate sulfotransferase activity
6.4
-
O88199
keratan sulfate sulfotransferase activity, assay at
6.8
-
-
imidazole buffer
6.8
-
O88199
chondroitin sulfotransferase activity, assay at
6.8
-
-
assay at
7.3
-
-
double pH-optimum: 100% at pH 6.0 and 65% at pH 7.3
8.2
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
9
-
pH 4.0: about 30% of activity maximum, pH 9.0: about 55% of activity maximum
additional information
-
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
37
-
-
assay at
37
-
O88199
assay at
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-, O88199
stromal cells
Manually annotated by BRENDA team
-
embryo chondrocytes release enzyme into the cell culture medium
Manually annotated by BRENDA team
-, O88199
-
Manually annotated by BRENDA team
Q9QZL2
perivascular, epineurial and perineurial
Manually annotated by BRENDA team
-, O88199
-
Manually annotated by BRENDA team
Q9QZL2
increased enzyme expression in mobile cells, downregulation accompanies declining mobility of Schwann cells as they engaged in the myelination of re-growing axons
Manually annotated by BRENDA team
-
from surgical disc procedures performed on individuals with herniated discs and degenerative disc disease
Manually annotated by BRENDA team
additional information
Q9QZL2
the C6st1 expression is upregulated GFAP-positive Schwann cells in an early phase of tissue reorganisation after nerve crush, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
enzyme is present in supernatant and in particulate fraction
-
Manually annotated by BRENDA team
additional information
-
embryo chondrocytes release enzyme into the medium under culture conditions
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
75000
-
Q92179
glycolysated enzyme, SDS-PAGE
150000
-
-
gel filtration
160000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 38000, SDS-PAGE
?
O88199
x * 67000, glycosylated recombinant enzyme, SDS-PAGE, x * 54000, deglycosylated recombinant enzyme, SDS-PAGE
dimer
-
2 * 75000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
Q92179
N-linked glycoprotein
glycoprotein
O88199
the enzyme contains N-linked oligosaccharide side chains, which have essential roles in the generation of the active enzyme and in its keratan sulfate sulfotransferase activity, overview, six potential N-glycosylation sites
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
thermal inactivation
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
homogeneity
-
partial
-
-
Q9EP78
partial
-
recombinant wild-type and mutant FLAG-tagged C6ST-1s from COS-7 cells by anti-FLAG-tag immunoaffinity and wheat germ agglutinin affinity chromatography
O88199
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
C6ST-2 isozyme
Q9NS84
gene CHST3, expression of Flag-tagged wild-type andmutant enzymes
-
gene CHST3, expression of wild-type enzyme and mutant L286P in COS-7 cells as soluble FLAG-tagged proteins
-
gene CHST3, gene structure, genotyping
-
gene C6st, expression of wild-type and mutant FLAG-tagged C6ST-1s in COS-7 cells, addition of tunicamycin completely abolishes N-glycosylation of the recombinant enzyme and enzyme activity, overview
O88199
gene C6st1, DNA and amino acid sequence determination and analysis, expression analysis and sequence comparison, expression of HIs-tagged full-length enzyme and truncated enzyme, lacking the N-terminal transmembrane domain but retaining the PAPS-binding sites and the catalytic domain, in COS-7 cells
Q9QZL2
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
L259P
-
naturally occuring mutation of CHST3 involved in enzyme deficiency and related disease
L286P
-
naturally occuring mutation of gene CHST3 causing the Spondyloepiphyseal dysplasia Omani type, almost inactive mutant, phenotype, overview
R222W
-
naturally occuring mutation of CHST3 involved in enzyme deficiency and related disease
R304Q
-
missense mutation found in patients with a distinct form of spondyloepiphyseal dysplasia, SED Omani type, complete loss of activity, resulting in significant reduction of both DELTAHexA-GalNAc(6S) and DELTAHexA(2S)-GalNAc(6S) and elevate levels of DELTAHexA-GalNAc(4S,6S)
R304Q
-
naturally occuring mutation of gene CHST3 causing the Spondyloepiphyseal dysplasia Omani type, phenotype, overview
T141M
-
homozygous missense mutation leading to a reduction of C6ST-1 activity to 24-29% of the wild type enzyme, the naturally occuring missense mutation in exon 3 of gene CHST3 causes Omani-type spondyloepiphyseal dysplasia with cardiac involvement, phenotype with skeletal features, cardiac involvement including mitral, tricuspid and/or aortic regurgitations and type E brachydactyly, overview
N250S
O88199
site-directed mutagenesis, mutation of a putative N-glycosylation site, deletion of the fourth N-glycosylation site from the N-terminus of the enzyme inhibits production of the active C6ST-1, the mutant remains in the endoplasmic reticulum, while all other wild-type and mutant enzymes are located in the Golgi apparatus
N413S
O88199
site-directed mutagenesis, mutation of a putative N-glycosylation site, deletion of the fourth or sixth N-glycosylation site from the N-terminus of the enzyme inhibits production of the active C6ST-1
N457S
O88199
site-directed mutagenesis, mutation of a putative N-glycosylation site, deletion of the fifth N-glycosylation site from the N-terminus of the enzyme results in a marked loss of the keratan sulfate sulfotransferase activity
N63S
O88199
site-directed mutagenesis, mutation of a putative N-glycosylation site
N74S
O88199
site-directed mutagenesis, mutation of a putative N-glycosylation site
L307P
-
naturally occuring mutation of CHST3 involved in enzyme deficiency and related disease
additional information
-
identification of eight naturally occuring CHST3 mutations in six unrelated individuals who presented at birth with congenital joint dislocations with diagnosis of either Larsen syndrome or humero-spinal dysostosis, the patients show reduced enzyme activity and congenital dislocation of the knees, elbow joint dysplasia with subluxation and limited extension, hip dysplasia or dislocation, clubfoot, short stature, and kyphoscoliosis developing in late childhood, genotyping, enzyme mutant phenotypes, overview
N96S
O88199
site-directed mutagenesis, mutation of a putative N-glycosylation site
additional information
Q9QZL2
enzyme upregulation facilitates Schwann cell migration during axonal growth, while enzyme downregulation accompanies declining mobility of Schwann cells as they engaged in the myelination of re-growing axons, overview
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
missense mutation R304Q found in patients with a distinct form of spondyloepiphyseal dysplasia, SED Omani type, leads to complete loss of activity, resulting in significant reduction of both DELTAHexA-GalNAc(6S) and DELTAHexA(2S)-GalNAc(6S) and elevated levels of DELTAHexA-GalNAc(4S,6S). Defect in chondroitin sulfate chain sulfation results in chondrodysplasia with major involvement of the spine