Information on EC 2.8.2.17 - chondroitin 6-sulfotransferase

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The expected taxonomic range for this enzyme is: Chordata

EC NUMBER
COMMENTARY
2.8.2.17
-
RECOMMENDED NAME
GeneOntology No.
chondroitin 6-sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
chondroitin sulfate biosynthesis (late stages)
-
Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:chondroitin 6'-sulfotransferase
The sulfation is at the 6-position of N-acetylgalactosamine residues of chondroitin. Not identical with EC 2.8.2.5 chondroitin 4-sulfotransferase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3'-phosphoadenosine 5'-phosphosulfate:chondroitin sulfate sulfotransferase
-
-
-
-
C6ST
-
-
-
-
C6ST-1
O88199
-
C6ST-1
Q9QZL2
-
carbohydrate sulfotransferase 3
Q7LGC8
-
chondroitin 6-O-sulfotransferase
-
-
-
-
chondroitin 6-O-sulfotransferase
-
-
chondroitin 6-O-sulfotransferase-1
Q7LGC8
-
chondroitin 6-sulfotransferase-1
O88199
-
chondroitin 6-sulphotransferase-1
Q9QZL2
-
chondroitin-6-sulfotransferase
-
-
CHST3
Q7LGC8
-
CST
-
-
-
-
sulfotransferase, chondroitin 6-
-
-
-
-
terminal 6-sulfotransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37292-93-6
-
83589-04-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
calf
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
gene CHST3
SwissProt
Manually annotated by BRENDA team
patients with a distinct form of spondyloepiphyseal dysplasia, SED Omani type
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
recombinant enzyme with FLAG-tag
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
gene Chst3, C6st, or Gst0; isozyme C6ST-1
SwissProt
Manually annotated by BRENDA team
wild-type and knock-out mutant
-
-
Manually annotated by BRENDA team
carbohydrate sulfotransferase 3; gene C6st1
SwissProt
Manually annotated by BRENDA team
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ba2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Ca2+
-
divalent cation required, Mn2+, Mg2+, Ca2+
Ca2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Co2+
-
good activator, stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Cu2+
-
can partially replace Mn2+ in activation
Cu2+
-
stimulates chondroitin 6-sulfotransferase activity but not keratan sulfate sulfotransferase activity
Mg2+
-
can partially replace Mn2+ in activation
Mg2+
-
divalent cation required, Mn2+, Mg2+, Ca2+
Mg2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Mn2+
-
stimulates
Mn2+
-
best activator
Mn2+
-
divalent cation required, Mn2+, Mg2+, Ca2+
Sr2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
Zn2+
-
can partially replace Mn2+ in activation
Mn2+
-
stimulates chondroitin 6-sulfotransferase activity and keratan sulfate sulfotransferase activity
additional information
-
Ca2+ has no effect
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Basic proteins
-
stimulate
-
Chondroitin sulfates
-
competitive inhibitors
Histone
-
stimulates, optimum concentration: 0.5 mg/ml
Lysozyme
-
stimulates, optimum concentration: 3.0 mg/ml
-
poly-L-lysine
-
stimulates 2fold at 1.5 mg/ml
Polyamines
-
stimulate
-
protamine
-
stimulates, optimum concentration: 0.025 mg/ml
protamine
-
stimulates 2fold at 1.5 mg/ml
protamine
-
12fold activation of enzyme activity with totally desulfated chondroitin, 3fold activation with partially sulfated chondroitin
protamine
-
-
spermidine
-
stimulates, optimum concentration: 2.5 mM
spermidine
-
stimulates 2fold at 1 mM
spermine
-
stimulates, optimum concentration: 0.6 mM
spermine
-
stimulates 2fold at 1 mM
Lysozyme
-
stimulates 2fold at 1.5 mg/ml
-
additional information
-
enzyme is present in supernatant and in particulate fraction, detergent activates indirectly by releasing enzyme into the medium
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
9
-
pH 4.0: about 30% of activity maximum, pH 9.0: about 55% of activity maximum
additional information
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-
-
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
75000
-
Q92179
glycolysated enzyme, SDS-PAGE
150000
-
-
gel filtration
160000
-
-
gel filtration
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
thermal inactivation
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE