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Information on EC 2.8.1.B3 - tRNA-5-methyluridine54 2-sulfurtransferase and Organism(s) Thermotoga maritima and UniProt Accession Q9X220

for references in articles please use BRENDA:EC2.8.1.B3
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Thermotoga maritima
UNIPROT: Q9X220
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The taxonomic range for the selected organisms is: Thermotoga maritima
The enzyme appears in selected viruses and cellular organisms
Synonyms
4-thiouridine synthetase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4-thiouridine synthetase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)
sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)
sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with a truncated tRNA, sitting drop vapor diffusion method, using 2 M sodium formate, 100 mM sodium citrate, pH 4.6, 2 mM dithiothreitol
in complex with RNA (truncated tRNAPhe), sitting drop vapor diffusion method, using 2 M sodium formate, 100 mM sodium citrate pH 4.6, 2 mM dithiothreitol, at 20Ā°C
sitting drop vapor diffusion method, using 2.0 M sodium formate, 100 mM sodium citrate pH 4.6, 2 mM dithiothreitol
THiI-tRNA complex and selenium-ThiI-tRNA complex, 3.1 A resolution
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose column chromatography and Superdex 75 gel filtration
MBP-fusion protein
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)pLysS cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Naumann, P.-T.
Versuche zur Strukturaufklärung bakterieller Thiouridin Synthetasen
PH. D. Thesis Universität Göttingen
2005
0000
2005
Escherichia coli, Thermotoga maritima
-
Manually annotated by BRENDA team
Naumann, P.T.; Lauhon, C.T.; Ficner, R.
Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex
Acta Crystallogr. Sect. F
69
421-424
2013
Thermotoga maritima (Q9X220), Thermotoga maritima
Manually annotated by BRENDA team
Neumann, P.; Lakomek, K.; Naumann, P.T.; Erwin, W.M.; Lauhon, C.T.; Ficner, R.
Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification
Nucleic Acids Res.
42
6673-6685
2014
Thermotoga maritima (Q9X220), Thermotoga maritima, Thermotoga maritima ATCC 43589 (Q9X220)
Manually annotated by BRENDA team