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Information on EC 2.7.9.2 - pyruvate, water dikinase and Organism(s) Pyrococcus furiosus and UniProt Accession P42850

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IUBMB Comments
A manganese protein.
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This record set is specific for:
Pyrococcus furiosus
UNIPROT: P42850
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Word Map
The taxonomic range for the selected organisms is: Pyrococcus furiosus
The enzyme appears in selected viruses and cellular organisms
Synonyms
phosphoenolpyruvate synthase, phosphoenolpyruvate synthetase, pep synthase, pep synthetase, phosphoenol pyruvate synthetase, water dikinase pyruvate, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoenolpyruvate synthase
-
phosphoenolpyruvate synthetase
-
kinase, pyruvate-water di- (phosphorylating)
-
-
-
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PEP synthase
-
-
-
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PEP synthetase
-
-
-
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phosphoenolpyruvate synthase
-
-
-
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phosphoenolpyruvate synthetase
-
-
-
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phosphoenolpyruvic synthase
-
-
-
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phosphopyruvate synthetase, phosphopyruvate
-
-
-
-
pyruvate, water dikinase
-
-
-
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pyruvate,water dikinase
-
-
-
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synthetase, phosphopyruvate
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-
-
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water dikinase pyruvate
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:pyruvate,water phosphotransferase
A manganese protein.
CAS REGISTRY NUMBER
COMMENTARY hide
9013-09-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
show the reaction diagram
-
-
-
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
show the reaction diagram
-
-
-
?
ATP + 2-ketobutyrate + H2O
?
show the reaction diagram
-
8% of the activity with pyruvate
-
-
?
ATP + 2-oxoglutarate + H2O
?
show the reaction diagram
-
2% of the activity with pyruvate
-
-
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
show the reaction diagram
additional information
?
-
-
no activity with 2-keto-3-methylvalerate, 2-ketoisocaproate, 2-ketomalonate, phenylpyruvate, hydroxyphenylpyruvate, imidazolpyruvate, and indole-3-pyruvate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
show the reaction diagram
-
involved in gluconeogenesis
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
enzyme contains 1 Ca atom per subunit
Co2+
-
requires divalent cations in the forward reaction, 93% of the activity with Mg2+
Iron
-
0.1-0.4 g-atoms per subunit, no functional part of the enzyme
K+
-
50% increased activity at 100 mM
Mn2+
-
requires divalent cations in the forward reaction, 95% of the activity with Mg2+
NH4+
-
48% increased activity at 100 mM
Ni2+
-
requires divalent cations in the forward reaction, 15% of the activity with Mg2+
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
not affected by Na+
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07
ATP
50°C, pH not specified in the publication
1
AMP
-
pH 8.4, 80°C
0.39
ATP
-
pH 8.4, 80°C
38.4
phosphate
-
pH 8.4, 80°C
0.4
phosphoenolpyruvate
-
pH 8.4, 80°C
0.11
pyruvate
-
pH 8.4, 80°C
additional information
additional information
-
kinetics
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.7
AMP
-
pH 8.4, 80°C
1330
ATP
-
pH 8.4, 80°C
11.9
phosphate
-
pH 8.4, 80°C
12.6
phosphoenolpyruvate
-
pH 8.4, 80°C
1570
pyruvate
-
pH 8.4, 80°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.05
50°C, pH not specified in the publication
14.9
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 90
-
optimum at 90°C, 0.2% of the optimal activity at 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
5% of total cytoplasmic protein
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
gluconeogenic enzyme
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
78000
x * 78000, SDS-PAGE
690000
-
gel filtration
92000
-
8 * 92000, SDS-PAGE, 30% of the enzyme is purified as a large inactive complex of about 1640 kDa
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 78000, SDS-PAGE
octamer
-
8 * 92000, SDS-PAGE, 30% of the enzyme is purified as a large inactive complex of about 1640 kDa
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90
-
t1/2: 9 h
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enhanced transcription of the mfrA gene in the presence of maltose
higher specific activity when cultures are grown in maltose medium
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hutchins, A.M.; Holden, J.F.; Adams, M.W.
Phosphoenolpyruvate synthetase from the hyperthermophilic archaeon Pyrococcus furiosus
J. Bacteriol.
183
709-715
2001
Pyrococcus furiosus
Manually annotated by BRENDA team
Sakuraba, H.; Utsumi, E.; Schreier, H.J.; Ohshima, T.
Transcriptional regulation of phosphoenolpyruvate synthase by maltose in the hyperthermophilic archaeon, Pyrococcus furiosus
J. Biosci. Bioeng.
92
108-113
2001
Pyrococcus furiosus (P42850), Pyrococcus furiosus
Manually annotated by BRENDA team
Schfer, T.; Schnheit, P.
Gluconeogenesis from pyruvate in the hyperthermophilic archaeon Pyrococcus furiosus involvement of reactions of the Embden-Meyerhof pathway
Arch. Microbiol.
159
354-363
1993
Pyrococcus furiosus (P42850)
-
Manually annotated by BRENDA team
Adams, M.; Holden, J.; Menon, A.; Schut, G.; Grunden, A.; Hou, C.; Hutchins, A.; Jenney F.E., J.; Kim, C.; Ma, K.; Pan, G.; Roy, R.; Sapra, R.; Story, S.; Verhagen, M.
Key role for sulfur in peptide metabolism and in regulation of three hydrogenases in the hyperthermophilic archaeon Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
Pyrococcus furiosus (P42850)
Manually annotated by BRENDA team