Information on EC 2.7.8.24 - phosphatidylcholine synthase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
2.7.8.24
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RECOMMENDED NAME
GeneOntology No.
phosphatidylcholine synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CDP-diacylglycerol + choline = CMP + phosphatidylcholine
show the reaction diagram
Requires divalent cations, with Mn2+ being more effective than Mg2+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Glycerophospholipid metabolism
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phosphatidylcholine biosynthesis VI
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phosphatidylethanolamine bioynthesis
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SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:choline O-phosphatidyltransferase
Requires divalent cations, with Mn2+ being more effective than Mg2+.
CAS REGISTRY NUMBER
COMMENTARY hide
243666-86-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain C58
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Borrelia burgdorferi
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Pseudomonas putida A ATCC 12633
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
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inhibitory at higher concentrations
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
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optimal activation at 0.2%, w/v
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Triton X-100
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned into the expression vector pET21b to form the recombinant plasmid pET125, expression in Escherichia coli BL21
Borrelia burgdorferi
expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli BL21(DE3).pLysS cells
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expressed in Escherichia coli BL21(DE3)/pLysS cells
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expressed in Escherichia coli DH5alpha cells
expression in Escherichia coli
from plasmid pGC41 in pGC36 for functional complementation in bacterial mutants lacking endogenous PcsA
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D56A
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inactive
D56E
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inactive
D56N
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inactive
D59A
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inactive
D59E
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inactive
D59N
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inactive
D81A
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inactive
D81E
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the mutant shows 14% of wild type activity
D81N
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the mutant shows 21% of wild type activity
D85A
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inactive
D85E
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inactive
D85N
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inactive
G229D
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inactive
G60A
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inactive
G77A
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inactive
G77D
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inactive
H20A
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inactive
H20L
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the mutant shows 62% of wild type activity
L72A
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the mutant shows 46% of wild type activity
L72R
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inactive
L80A
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the mutant shows 85% of wild type activity
R64A
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the mutant shows 67% of wild type activity
T23A
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inactive
T23S
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the mutant shows 67% of wild type activity
V83A
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the mutant shows 39% of wild type activity
W142A
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inactive
Y123A
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the mutant shows less than 20% of wild type activity
Y86A
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inactive
Y89A
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inactive
Show AA Sequence (872 entries)
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