Information on EC 2.7.8.24 - phosphatidylcholine synthase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
2.7.8.24
-
RECOMMENDED NAME
GeneOntology No.
phosphatidylcholine synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
CDP-diacylglycerol + choline = CMP + phosphatidylcholine
show the reaction diagram
Requires divalent cations, with Mn2+ being more effective than Mg2+
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Glycerophospholipid metabolism
-
phosphatidylcholine biosynthesis VI
-
SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:choline O-phosphatidyltransferase
Requires divalent cations, with Mn2+ being more effective than Mg2+.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
CDP-diglyceride-choline O-phosphatidyltransferase
-
-
-
-
CDPdiglyceride-choline O-phosphatidyltransferase
-
-
-
-
PC synthase
-
-
-
-
PCS
Fluoribacter bozemanae ATCC 33217
D5KX81
-
-
PCS
Pseudomonas putida A ATCC 12633
-
-
-
PCS
E9NR86
-
PCS
E9NR86
-
-
PcsA
-
lidK/pcsA gene product
phosphatidylcholine synthase
-
-
CAS REGISTRY NUMBER
COMMENTARY
243666-86-6
-
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
a deletion mutant is much more sensitive than the wild type strain when challenged with Al3+ and tetradecyltrimethylammonium bromide, and shows a significant loss of viability
malfunction
Pseudomonas putida A ATCC 12633
-
a deletion mutant is much more sensitive than the wild type strain when challenged with Al3+ and tetradecyltrimethylammonium bromide, and shows a significant loss of viability
-
metabolism
-
the enzyme is responsible for the synthesis of phosphatidylcholine, which acts as a temporary reservoir for Al3+
metabolism
Pseudomonas putida A ATCC 12633
-
the enzyme is responsible for the synthesis of phosphatidylcholine, which acts as a temporary reservoir for Al3+
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-, O51265
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-, Q8YC37
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
E9NR86, -
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
D5KX81
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
Q9HXE9
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
second bacterial pathway for phosphatidylcholine biosynthesis
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
Q9KJY8
second bacterial pathway for phosphatidylcholine biosynthesis
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
Q8YC37
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
the phosphatidylcholine synthase gene is solely responsible for the ability of the organism to synthesize phosphatidylcholine
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
phosphatidylcholine synthesis important for functionality of Dot/Icm translocator (formation of replicative vacuole), binding to macrophages, sufficient level of flagellin protein (linked to bacterial cytotoxicity, cellular adhesion)
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
overexpression of PcsA complements low phosphatidylcholine levels in membrane of mutant strain lacking PcsA or lacking both PcsA and PmtA (phospholipid N-methyltransferase)
lipid analysis by thin-layer chromatography
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
Fluoribacter bozemanae ATCC 33217
D5KX81
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
E9NR86
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
Pseudomonas putida A ATCC 12633
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
second bacterial pathway for phosphatidylcholine biosynthesis
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
Q9KJY8
second bacterial pathway for phosphatidylcholine biosynthesis
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
Q8YC37
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
the phosphatidylcholine synthase gene is solely responsible for the ability of the organism to synthesize phosphatidylcholine
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
show the reaction diagram
-
phosphatidylcholine synthesis important for functionality of Dot/Icm translocator (formation of replicative vacuole), binding to macrophages, sufficient level of flagellin protein (linked to bacterial cytotoxicity, cellular adhesion)
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
20fold less effective than Mn2+
Mg2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mg2+
Q9HXE9
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mg2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mn2+
-
optimal activation at 10 mM, more effective than Mg2+
Mn2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
Mn2+
Q9HXE9
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
Mn2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Triton X-100
-
inhibitory at higher concentrations
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Triton X-100
-
optimal activation at 0.2%, w/v
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
a transmembrane protein
Manually annotated by BRENDA team
Q9HXE9
an integral membrane protein
Manually annotated by BRENDA team
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Triton X-100
-
the presence of Triton X-100 is required in order to detect enzyme activity. 0.2% (w/v) Triton X-100 gives maximal enzyme activity
Triton X-100
Q9HXE9
the presence of Triton X-100 is required in order to detect enzyme activity. 0.2% (w/v) Triton X-100 gives maximal enzyme activity
Triton X-100
-
the presence of Triton X-100 is required in order to detect enzyme activity. 0.2% (w/v) Triton X-100 gives maximal enzyme activity
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cloned into the expression vector pET21b to form the recombinant plasmid pET125, expression in Escherichia coli BL21
-, O51265
expression in Escherichia coli
-
expression in Escherichia coli
Q8YC37
expressed in Escherichia coli DH5alpha cells
D5KX81
expression in Escherichia coli
-
from plasmid pGC41 in pGC36 for functional complementation in bacterial mutants lacking endogenous PcsA
-
expression in Escherichia coli
-
expression in Escherichia coli
-
expressed in Escherichia coli BL21(DE3)/pLysS cells
-
expressed in Escherichia coli BL21(DE3) pLysS cells
E9NR86, -
expressed in Escherichia coli BL21(DE3).pLysS cells
-
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D56A
-
inactive
D56E
-
inactive
D56N
-
inactive
D59A
-
inactive
D59E
-
inactive
D59N
-
inactive
D81A
-
inactive
D81E
-
the mutant shows 14% of wild type activity
D81N
-
the mutant shows 21% of wild type activity
D85A
-
inactive
D85E
-
inactive
D85N
-
inactive
G229D
-
inactive
G60A
-
inactive
G77A
-
inactive
G77D
-
inactive
H20A
-
inactive
H20L
-
the mutant shows 62% of wild type activity
L72A
-
the mutant shows 46% of wild type activity
L72R
-
inactive
L80A
-
the mutant shows 85% of wild type activity
R64A
-
the mutant shows 67% of wild type activity
T23A
-
inactive
T23S
-
the mutant shows 67% of wild type activity
V83A
-
the mutant shows 39% of wild type activity
W142A
-
inactive
Y123A
-
the mutant shows less than 20% of wild type activity
Y86A
-
inactive
Y89A
-
inactive