Information on EC 2.7.8.23 - carboxyvinyl-carboxyphosphonate phosphorylmutase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.8.23
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RECOMMENDED NAME
GeneOntology No.
carboxyvinyl-carboxyphosphonate phosphorylmutase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-carboxyvinyl carboxyphosphonate = 3-(hydrohydroxyphosphoryl)pyruvate + CO2
show the reaction diagram
catalyses the transfer and decarboxylation of the carboxy(hydroxy)phosphoryl group, HOOC-P(O)(OH)-(phosphoryl being a 3-valent group), in the formation of an unusual C-P bond that is involved in the biosynthesis of the antibiotic bialaphos
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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-
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substituted phospho group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycolysis
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phosalacine biosynthesis
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phosphinothricin tripeptide biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
1-carboxyvinyl carboxyphosphonate phosphorylmutase (decarboxylating)
Catalyses the transfer and decarboxylation of the carboxy(hydroxy)phosphoryl group, HOOC-P(O)(OH)- (phosphoryl being a 3-valent group), in the formation of an unusual C-P bond that is involved in the biosynthesis of the antibiotic bialaphos.
CAS REGISTRY NUMBER
COMMENTARY hide
122799-57-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Streptomyces hygroscopicus
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme plays a role that is related to the tillering regulation in rice
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-carboxyvinyl carboxyphosphonate
3-(hydrohydroxyphosphoryl)pyruvate + CO2
show the reaction diagram
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-
-
?
carboxyphosphinopyruvate
(hydroxyphosphinyl)pyruvate + CO2
show the reaction diagram
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-
-
?
carboxyphosphoenolpyruvate
?
show the reaction diagram
-
-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-carboxyvinyl carboxyphosphonate
3-(hydrohydroxyphosphoryl)pyruvate + CO2
show the reaction diagram
Q2QWN6
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-
-
?
carboxyphosphonoenolpyruvate
(hydroxyphosphinyl)pyruvate + CO2
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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NaCl, KCl and NH4(SO4)2 up to 100 mM have no effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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enzyme is inactive in presence of EDTA, but inhibition is completely relieved by addition of 1 mM MnSO4 or MgSO4
Phosphonopyruvate
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5 mM, 50% inhibition
additional information
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phosphoenolpyruvate shows almost no effect
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0022
carboxyphosphinopyruvate
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pH 6.5, 30C
0.25 - 0.27
carboxyphosphoenolpyruvate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00076
carboxyphosphinopyruvate
Streptomyces hygroscopicus
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pH 7.5, 30C
0.02
carboxyphosphoenolpyruvate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32700
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calculated from amino acid composition
32800
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calculated from amino acid composition
56000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 38000, calculated from amino acid sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8
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stable in this range
643769, 643773
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 80% ammonium sulfate suspension, no loss of activity observed for 1 month
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4C, 1 mg/ml in 50 mM MES buffer, pH 6.5, stable for 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned and expressd at high levels in Escherichia coli
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gene cloned from a subgenomic library cloned by colony hybridisation in Escherichia coli AG1, subcloned into vector pET11a and overexpressed in Escherichia coli BL21(DE3)
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mutant NP71 constructed by gene replacement technique
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nucleotide sequence of gene isolated and cloned and expressed in Streptomyces lividans 66 using expression vector pIJ680
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the mRNA level is down-regulated in the high-tillering d10 dwarf plants
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
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