Information on EC 2.7.8.23 - carboxyvinyl-carboxyphosphonate phosphorylmutase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.7.8.23
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RECOMMENDED NAME
GeneOntology No.
carboxyvinyl-carboxyphosphonate phosphorylmutase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
1-carboxyvinyl carboxyphosphonate = 3-(hydrohydroxyphosphoryl)pyruvate + CO2
show the reaction diagram
catalyses the transfer and decarboxylation of the carboxy(hydroxy)phosphoryl group, HOOC-P(O)(OH)-(phosphoryl being a 3-valent group), in the formation of an unusual C-P bond that is involved in the biosynthesis of the antibiotic bialaphos
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
decarboxylation
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-
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substituted phospho group transfer
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PATHWAY
KEGG Link
MetaCyc Link
phosphinothricin tripeptide biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
1-carboxyvinyl carboxyphosphonate phosphorylmutase (decarboxylating)
Catalyses the transfer and decarboxylation of the carboxy(hydroxy)phosphoryl group, HOOC-P(O)(OH)- (phosphoryl being a 3-valent group), in the formation of an unusual C-P bond that is involved in the biosynthesis of the antibiotic bialaphos.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
carboxyPEP mutase
Q2QWN6
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carboxyphosphoenolpyruvate mutase
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carboxyphosphonoenolpyruvate phosphonomutase
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CPEP mutase
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CPEP mutase
Q2QWN6
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CPEP phosphonomutase
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CPPM
Q2QWN6
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CAS REGISTRY NUMBER
COMMENTARY
122799-57-9
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
no activity in Streptomyces hygroscopicus
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Manually annotated by BRENDA team
HP782 and mutant NP71; SF1293
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Manually annotated by BRENDA team
HP782, HP5-29 and SF1293 mutant NP71; SF1293
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Manually annotated by BRENDA team
Streptomyces hygroscopicus SF1293
SF1293
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Manually annotated by BRENDA team
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
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increases activity, 1-10 mM MgSO4, relative activity 250%
Mg2+
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reaction shows metal dependence
Mn2+
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1 mM MnSO4, relative activity 680%, 10 mM MnSO4, relative activity 600%
Mn2+
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increases activity, about 10times more active compared with the activity in presence of Mg2+
additional information
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NaCl, KCl and NH4(SO4)2 up to 100 mM have no effect
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Phosphonopyruvate
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5 mM, 50% inhibition
EDTA
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enzyme is inactive in presence of EDTA, but inhibition is completely relieved by addition of 1 mM MnSO4 or MgSO4
additional information
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phosphoenolpyruvate shows almost no effect
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KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0022
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carboxyphosphinopyruvate
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pH 6.5, 30C
0.25
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carboxyphosphoenolpyruvate
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pH 6.5, 27C
0.27
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carboxyphosphoenolpyruvate
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0.27
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carboxyphosphoenolpyruvate
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pH 6.5, 30C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00076
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carboxyphosphinopyruvate
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pH 7.5, 30C
0.02
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carboxyphosphoenolpyruvate
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0.02
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carboxyphosphoenolpyruvate
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pH 7.5, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
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specific activity 217.0 U/mg of protein, one unit is the amount of enzyme required to produce 1micromol of bialaphos
additional information
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specific activity 37.6 U/mg x 1000
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
PDB
SCOP
CATH
ORGANISM
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
32700
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calculated from amino acid composition
32800
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calculated from amino acid composition
56000
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gel filtration
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
8
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stable in this range
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 80% ammonium sulfate suspension, no loss of activity observed for 1 month
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4C, 1 mg/ml in 50 mM MES buffer, pH 6.5, stable for 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cloned and expressd at high levels in Escherichia coli
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gene cloned from a subgenomic library cloned by colony hybridisation in Escherichia coli AG1, subcloned into vector pET11a and overexpressed in Escherichia coli BL21(DE3)
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mutant NP71 constructed by gene replacement technique
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nucleotide sequence of gene isolated and cloned and expressed in Streptomyces lividans 66 using expression vector pIJ680
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APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
agriculture
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formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
agriculture
Streptomyces hygroscopicus SF1293
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formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production; formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production
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