Information on EC 2.7.8.11 - CDP-diacylglycerol-inositol 3-phosphatidyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.7.8.11
-
RECOMMENDED NAME
GeneOntology No.
CDP-diacylglycerol-inositol 3-phosphatidyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
CDP-diacylglycerol + myo-inositol = CMP + 1-phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
substituted phospho group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
3-phosphoinositide biosynthesis
-
D-myo-inositol (1,4,5)-trisphosphate biosynthesis
-
Glycerophospholipid metabolism
-
Inositol phosphate metabolism
-
Metabolic pathways
-
phospholipid biosynthesis II
-
SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:myo-inositol 3-phosphatidyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
BnPtdIns S1
Q8LKS7
-
cdipt
-
-
CDP-DG:inositol transferase
-
-
-
-
CDP-diacylglacerol: myo-inositol 3-phosphatidyl-transferase
C0H5B7
-
CDP-diacylglacerol: myo-inositol 3-phosphatidyl-transferase
Q2MJB8
-
CDP-diacylglycerol-inositol 3-phosphatidyltransferase
-
gene name
CDP-diacylglycerol-inositol phosphatidyltransferase
-
-
-
-
CDP-diacylglycerol:myo-inositol-3-phosphatidyltransferase
-
-
-
-
CDP-diglyceride-inositol phosphatidyltransferase
-
-
-
-
CDP-diglyceride-inositol transferase
-
-
-
-
CDP-diglyceride:inositol transferase
-
-
-
-
CDPdiglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine 5'-diphospho-1,2-diacyl-sn-glycerol: myoinositol 3-phosphatidyl transferase
-
-
cytidine 5'-diphospho-1,2-diacyl-sn-glycerol:myo-inositol 3-phosphatidyltransferase
-
-
-
-
cytidine diphosphodiglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine diphosphoglyceride-inositol phosphatidyltransferase
-
-
-
-
cytidine diphosphoglyceride-inositol transferase
-
-
-
-
PfPIS
C0H5B7
-
phosphatidyinositol synthase
Q8GUK6, Q8LBA6
-
phosphatidyinositol synthase
C0H5B7
-
phosphatidyinositol synthase
Q2MJB8
-
phosphatidyinositol synthase
-
-
phosphatidyinositol synthase
Tetrahymena vorax V2S
-
-
-
phosphatidylinositol synthase
-
-
-
-
phosphatidylinositol synthase 1
-
-
phosphatidylinositol synthase1
Q8LKS7
-
PI synthase
-
-
PI synthase
C0H5B7
-
PI synthase
-
-
PI synthase
Q2MJB8
-
PIS1
Q8LBA6
gene locus At1g68000
PIS1-encoded phosphatidylinositol synthase
-
-
PIS2
Q8GUK6
gene locus At4g38570
PkPIS
Q2MJB8
-
PtdIn synthase
-
-
PtdIn synthase
Tetrahymena vorax V2S
-
-
-
PtdIns synthase
Q8GUK6, Q8LBA6
-
PtdIns synthase
-
-
PtdIns synthase
-
-
PtIns synthase
-
-
PtIns synthase
Toxoplasma gondii 76K
-
-
-
ZmPIS
-
-
CAS REGISTRY NUMBER
COMMENTARY
9027-01-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
; gene AtPIS1, cDNA
-
-
Manually annotated by BRENDA team
ecotype Columbia-0
UniProt
Manually annotated by BRENDA team
ecotype Columbia:2; gene AtPIS1, cDNA
-
-
Manually annotated by BRENDA team
gene AtPIS2, putative phosphatidylinositol synthase that appears to be encoded by a genomic fragment present in BAC clone F20m13
UniProt
Manually annotated by BRENDA team
-
Q8LKS7
SwissProt
Manually annotated by BRENDA team
chicken
-
-
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
cell line A431; human
-
-
Manually annotated by BRENDA team
human
Uniprot
Manually annotated by BRENDA team
no activity in Escherichia coli
-
-
-
Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
yeast, PI synthase mutant strain D501-1
-
-
Manually annotated by BRENDA team
rabbit, New Zealand white
-
-
Manually annotated by BRENDA team
strain 3D7
UniProt
Manually annotated by BRENDA team
erythrocytes infected with
-
-
Manually annotated by BRENDA team
castor bean
-
-
Manually annotated by BRENDA team
wild-type strain S288C(alpha gal2); yeast
-
-
Manually annotated by BRENDA team
; strain V2S
-
-
Manually annotated by BRENDA team
Tetrahymena vorax V2S
strain V2S
-
-
Manually annotated by BRENDA team
strain 76K, cDNA fragment TgPIS1 encoding PtIns synthase
-
-
Manually annotated by BRENDA team
strain 76K, cDNA fragment TgPIS2 potentially encoding PtIns synthase
SwissProt
Manually annotated by BRENDA team
Toxoplasma gondii 76K
strain 76K, cDNA fragment TgPIS1 encoding PtIns synthase
-
-
Manually annotated by BRENDA team
Toxoplasma gondii 76K
strain 76K, cDNA fragment TgPIS2 potentially encoding PtIns synthase
SwissProt
Manually annotated by BRENDA team
maize, L. cv. Mona
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
using a cdipt knockout it shown that cdipt is required for lens structural integrity and photoreceptor cell survival in the zebrafish eye
malfunction
-
overexpression of ZmPIS leads to elevated levels of phospholipids and galactolipids in transgenic tobacco leaves under drought stress conditions compared to that of wild-type. These changes are correlated with increased drought tolerance; ZmPIS complements the yeast PIS1 mutant phenotype (nonviable on medium with or without lysine)
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-stearoyl-2-arachidonoylphosphatidylinositol + CMP
CDP-1-stearoyl-2-arachidonoylglycerol + myo-inositol
show the reaction diagram
-
-
-
r
2-deoxy-CDP-diacylglycerol + myo-inositol
CMP + ?
show the reaction diagram
-
-
-
-
r
2-deoxy-CDP-diacylglycerol + myo-inositol
CMP + ?
show the reaction diagram
-
optimal activity is 25% higher then with CDP-diacylglycerol
-
-
r
2-deoxy-CDP-diacylglycerol + myo-inositol
CMP + ?
show the reaction diagram
-
maximal activity only 36% of the optimal activity reached with CDP-diacylglycerol
-
-
r
CDP-1-arachidonoyl-2-stearoylglycerol + myo-inositol
CMP + 1-arachidonoyl-2-stearoylphosphtidylinositol
show the reaction diagram
-
6% of the activity with CDP-1-stearoyl-2-oleoylglycerol
-
-
r
CDP-1-stearoyl-2-arachidonoylglycerol + myo-inositol
CMP + 1-stearoyl-2-arachidonoylphosphatidylinositol
show the reaction diagram
-
4% of the activity with CDP-1-stearoyl-2-oleoylglycerol
-
-
r
CDP-1-stearoyl-2-oleoylglycerol + myo-inositol
CMP + 1-stearoyl-2-oleoylphosphatidylinositol
show the reaction diagram
-
-
-
-
r
CDP-diacylglycerol + 1D-chiro-inositol
CMP + phosphatidyl-1D-chiro-inositol
show the reaction diagram
-
conversion of 30.9% CDP-diacylglycerol (utilization significant compared to background, p: 0.05), microsomal fraction, 30C, 20 min
-
-
?
CDP-diacylglycerol + 1L-chiro-inositol
CMP + phosphatidyl-1L-chiro-inositol
show the reaction diagram
-
conversion of 25.1% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
-
-
?
CDP-diacylglycerol + allo-inositol
CMP + phosphatidyl-allo-inositol
show the reaction diagram
-
83% of the activity on myo-inositol, conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
-
-
?
CDP-diacylglycerol + allo-inositol
CMP + phosphatidyl-allo-inositol
show the reaction diagram
Tetrahymena vorax V2S
-
83% of the activity on myo-inositol, conversion of 23.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
-
-
?
CDP-diacylglycerol + D-chiro-inositol
CMP + phosphatidyl-chiro-inositol
show the reaction diagram
-
82% of the activity on myo-inositol
-
-
?
CDP-diacylglycerol + D-myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
Q8GUK6, Q8LBA6
key step in lipid biosynthesis
-
-
?
CDP-diacylglycerol + D-myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
Q8GUK6, Q8LBA6
D-myo-inositol substrates: phosphatidylethanolamine, phosphatidic acid, overexpressed enzyme in bacterial membrane pellets, 30 min, 22C, pH 8, 1% (v/v) Triton X-100, 20 mM MgCl2
fatty acid analysis by thin-layer chromatography
-
?
CDP-diacylglycerol + epi-inositol
CMP + phosphatidyl-epi-inositol
show the reaction diagram
-
62% of the activity on myo-inositol, conversion of 31.1% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30C, 20 min
-
-
?
CDP-diacylglycerol + L-chiro-inositol
CMP + phosphatidyl-chiro-inositol
show the reaction diagram
-
67% of the activity on myo-inositol
-
-
?
CDP-diacylglycerol + muco-inositol
CMP + phosphatidyl-muco-inositol
show the reaction diagram
-
41% of the activity on myo-inositol, conversion of 15.2% CDP-diacylglycerol (non-significant), microsomal fraction, 30C, 20 min
-
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
O14735
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-, Q8GUK6
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
Q9XY91, -
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
formation of membrane phospholipids essential for the growth
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
key enzyme in cell membrane biosynthesis
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
membrane lipid biosynthesis
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme plays a role in resynthesis of phosphatidylinositol during agonist-stimulated inositol-phospholipid metabolism
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
phosphatidylinositol synthase activity increased when zinc is depleted from the growth medium. Analysis of a zrt1DELTA zrt2DELTA mutant defective in plasma membrane zinc transport indicates that the cytoplasmic levels of zinc are responsible for the regulation of phosphatidylinositol synthase. Regulation of PIS1 gene expression by zinc depletion is mediated by the zinc-regulated transcription factor Zap1p. Direct in-teraction between glutathione S-transferase (GST)-Zap1p 687880 and a putative upstream activating sequence (UAS) zinc-responsive element in the PIS1
-
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
Q6KBS8
high specificityfor myo-inositol
-
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
Toxoplasma gondii 76K
Q9XY91, -
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-myo-inositol
show the reaction diagram
-
myo-inositol and scyllo-inositol are preferred substrates
-
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-myo-inositol
show the reaction diagram
-
conversion of 37.4% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, standard reaction: 20 min, 30C, pH 8, 2 mM MgCl2, 2 mM MnCl2, 1.6 mM Triton X-100
analyses by liquid scintillation counting and thin-layer chromatography
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-myo-inositol
show the reaction diagram
C0H5B7, -, Q2MJB9
parasite membrane extracts, infected red blood cells
-
-
?
CDP-diacylglycerol + scyllo-inositol
CMP + phosphatidyl-scyllo-inositol
show the reaction diagram
-
myo-inositol and scyllo-inositol are preferred substrates, conversion of 33.7% CDP-diacylglycerol (utilization significant compared to background, p: 0.01), microsomal fraction, 30C, 20 min
-
-
?
CDP-diarachidonoylglycerol + myo-inositol
CMP + diarachidonoylphosphatidylinositol
show the reaction diagram
-
9% of the activity with CDP-1-stearoyl-2-oleoylglycerol
-
-
r
CDP-didecanoyl-sn-glycerol + myo-inositol
CMP + didecanoylphosphatidylinositol
show the reaction diagram
-
preferred substrate
-
-
r
CDP-diheptadecanoylglycerol + D-myo-inositol
CMP + 1,2-diheptadecanoyl-phosphatidyl-1D-myo-inositol
show the reaction diagram
Q8GUK6, Q8LBA6
-
-
-
?
CDP-dilinoleoylglycerol + D-myo-inositol
CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol
show the reaction diagram
Q8GUK6, Q8LBA6
-
-
-
?
CDP-dilinoleoylglycerol + D-myo-inositol
CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol
show the reaction diagram
Q8GUK6, Q8LBA6
highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dioleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol
-
-
?
CDP-dioleoylglycerol + D-myo-inositol
CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol
show the reaction diagram
Q8GUK6, Q8LBA6
-
-
-
?
CDP-dioleoylglycerol + D-myo-inositol
CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol
show the reaction diagram
Q8GUK6, Q8LBA6
highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dilinoleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol
-
-
?
CDP-dioleoylglycerol + myo-inositol
CMP + dioleoylphosphatidylinositol
show the reaction diagram
-
-
-
r
CDP-dioleoylglycerol + myo-inositol
CMP + dioleoylphosphatidylinositol
show the reaction diagram
-
-
-
r
CDP-dipalmitoyl-sn-glycerol + myo-inositol
CMP + dipalmitoylphosphatidylinositol
show the reaction diagram
-
-
-
-
r
CDP-dipalmitoylglycerol + D-myo-inositol
CMP + 1,2-dipalmitoyl-phosphatidyl-1D-myo-inositol
show the reaction diagram
Q8GUK6, Q8LBA6
-
-
-
?
CDP-distearoylglycerol + myo-inositol
CMP + distearoylphosphatidylinositol
show the reaction diagram
-
38% of the activity with CDP-1-stearoyl-2-oleoylglycerol
-
-
r
CDPdipalmitoyl-sn-glycerol + myo-inositol
CMP + dipalmitoylphosphatidylinositol
show the reaction diagram
-
70% of the activity with CDP-1-stearoyl-2-oleoylglycerol
-
-
r
additional information
?
-
-
-
-
-
-
additional information
?
-
-
CDP, CTP, UMP, AMP, and GMP, are no substrates
-
-
-
additional information
?
-
-
UDP-diacylglycerol, ADP-diacylglycerol, and GDP-diacylglycerol are inactive as substrates
-
-
-
additional information
?
-
-
enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol
-
-
-
additional information
?
-
-
enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol
-
-
-
additional information
?
-
-
enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol
-
-
-
additional information
?
-
-
enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol
-
-
-
additional information
?
-
-
formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol
-
-
?
additional information
?
-
Q8GUK6, Q8LBA6
highly efficient conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic, acid linoleic acid and linolenic acid, complete conversion of CDP-diacylglycerol residues containing palmitic, acid linoleic acid and linolenic acid
-
-
-
additional information
?
-
Q8GUK6, Q8LBA6
in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, and polyphosphoinositide (PtdIns(4,5)P, PtdIns(4,5)P2) that are enriched in polyunsaturated fatty acids compared to wild-type plants
-
-
-
additional information
?
-
Q8GUK6, Q8LBA6
in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, phosphatidylethanolamine and diacylglycerol containing less polyunsaturated fatty acids but more saturated and monounsaturated fatty acids, no change in fatty acid composition of polyphopshoinositides compared to wild-type plants
-
-
-
additional information
?
-
-
microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol
-
-
-
additional information
?
-
Q8GUK6, Q8LBA6
moderate conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic acid and little linoleic acid, non-converted CDP-diacylglycerol residues containing linoleic acid and linolenic acid
-
-
-
additional information
?
-
-
myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol
-
-
-
additional information
?
-
C0H5B7, -, Q2MJB9
partial complementation of PIS activity by overexpression of PfPIS/ScPIS hybrids in ScPIS1-deficient Saccharomyces cerevisiae mutant strain BY4743, analysis by enzyme assay (30C, yeast membrane pellet, in presence of 18 mM octyl-beta-D-glucopyranoside) or lipid incorporation and extraction
-
-
-
additional information
?
-
Q8GUK6, Q8LBA6
preference of C18-fatty acid CDP-diacylglycerol substrates
-
-
-
additional information
?
-
Q8GUK6, Q8LBA6
preference of polyansaturated C18-fatty acid CDP-diacylglycerol substrates
-
-
-
additional information
?
-
Tetrahymena vorax V2S
-
formation of novel non-myo-inositol-containing second messengers, preferred non-myo-inositols are scyllo- and chiro-inositol
-
-
?
additional information
?
-
Tetrahymena vorax V2S
-
microsomal fraction contains additional PdtIn: inositol phosphatidyl transferase activity (inositol headgroup exchange, 20 min, 30C) between myo-, scyllo-, 1D-chiro-inositol and phosphatidyl-myo-inositol, increased in presence of CMP, least specificity for 1L-chiro- and muco-inositol, myo- and scyllo-inositol preferred inositol isomers for PtdIn synthase activity, least specificity for muco-inositol
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + D-myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
Q8GUK6, Q8LBA6
key step in lipid biosynthesis
-
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
O14735
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-, Q8GUK6
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
Q9XY91, -
-
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
formation of membrane phospholipids essential for the growth
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
key enzyme in cell membrane biosynthesis
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
membrane lipid biosynthesis
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
enzyme plays a role in resynthesis of phosphatidylinositol during agonist-stimulated inositol-phospholipid metabolism
-
r
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
-
phosphatidylinositol synthase activity increased when zinc is depleted from the growth medium. Analysis of a zrt1DELTA zrt2DELTA mutant defective in plasma membrane zinc transport indicates that the cytoplasmic levels of zinc are responsible for the regulation of phosphatidylinositol synthase. Regulation of PIS1 gene expression by zinc depletion is mediated by the zinc-regulated transcription factor Zap1p. Direct in-teraction between glutathione S-transferase (GST)-Zap1p 687880 and a putative upstream activating sequence (UAS) zinc-responsive element in the PIS1
-
-
?
CDP-diacylglycerol + myo-inositol
CMP + phosphatidyl-1D-myo-inositol
show the reaction diagram
Toxoplasma gondii 76K
Q9XY91, -
-
-
r
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
either Mg2+ or Mn2+ ions are necessary
Mg2+
-
either Mg2+ or Mn2+ ions are necessary
Mg2+
-
reaction and reverse reaction dependent on either Mg2+ or Mn2+
Mg2+
-
either Mg2+ or Mn2+ ions are necessary
Mg2+
-
reaction and reverse reaction dependent on either Mg2+ or Mn2+
Mg2+
Q9XY91, -
-
Mg2+
-, Q8GUK6
-
Mg2+
-
less effect at more than 5 mM; stimulation, maximum catalytic activity in presence of 2 mM MgCl2 plus 2 mM MnCl2. Less stimulatory above 5 mM
Mn2+
-
either Mn2+ or Mg2+ ions are necessary
Mn2+
-
-
Mn2+
-
either Mn2+ or Mg2+ ions are necessary
Mn2+
-
reaction and reverse reaction dependent on either Mn2+ or Mg2+
Mn2+
-
absolute requirement, reaction is dependent on for maximum activity
Mn2+
Q9XY91, -
-
Mn2+
-
greater effect than Mg2+, less effect at more than 5 mM; stimulation, maximum catalytic activity in presence of 2 mM MgCl2 plus 2 mM MnCl2. Less stimulatory above 5 mM
additional information
-
replacement of Mn2+ or Mg2+ with 1 mM Co2+ give approximately 10% of maximal activity, 1 mM Zn2+, Ni2+, Cu2+, Ca2+ and Fe2+ does not activate the enzyme
additional information
-
Ca2+ is ineffective
additional information
-
slight or no effect by calcium, cobalt, copper, iron, lithium, molybdenum, zinc ions; synergistic effect of Mg2+ and Mn2+, optimum condition: 2 mM MgCl2, 2 mM MnCl2
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
6-O-(N-heptyl-carbamoyl)methyl-alpha-D-glucopyranoside
-
Hecameg, above 5 mM
AMP
-
non-competitive inhibition
Bovine serum albumin
-
slight inhibition
-
Ca2+
-
markedly inhibits about 0.5 mM
Ca2+
-
inhibition above 2 mM
Ca2+
-
inhibition above 2 mM
CDP
-
competitive inhibition
CDP-diacylglycerol
-
-
CDP-diacylglycerol
-
above 0.5 mM
CDP-diacylglycerol
-
above 0.4 mM
CDP-dipalmitoylglyceride
-
above 1.2 mM
Cetylpyridinium chloride
-
-
Cetyltrimethylammonium bromide
-
strong inhibition
diphosphate
-
most efficient inhibitor
hexachlorocyclohexane
-
-
hexachlorocyclohexane
-
-
hexachlorocyclohexane
-
inhibition is non-competitive and not specific for inositol processing enzymes
Inosose-2
-
competitive to inositol
Inostamycin
-
-
iodoacetamide
-
-
myo-Inositol
-
product inhibition
myo-Inositol
-
substrate inhibition above 1 mM
N-ethylmaleimide
-
-
p-chloromercuribenzene sulfonate
-
-
p-chloromercuribenzene sulfonate
-
-
Sodium dodecyl sulfate
-
-
Triton X-100
-
inhibits at all concentrations tested
Triton X-100
-
above 1%
additional information
-
inactivated by thioreactive reagents
-
additional information
-
AMP CMP, GMP, UMP, TMP, TDP, and GDP are not inhibitory, triphosphates are even more inhibitory than diphosphates
-
additional information
-
no substrate inhibition with 2-deoxy-CDPdiacylglycerol, CMP and to a lesser extent AMP shows low inhibition of the enzyme
-
additional information
-
AMP CMP, GMP, UMP, TMP, TDP, and GDP are not inhibitory, triphosphates are even more inhibitory than diphosphates; no inhibition with 2-deoxy-CDP-diacylglycerol
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
enhances enzyme activity
Triton X-100
-
-
Triton X-100
-
-
Triton X-100
-
0.1% increases activity by 15fold
Triton X-100
-
-
Triton X-100
-
reaction is dependent on for maximum activity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.045
-
1-stearoyl-2-arachidonoylglycerol
-
pH 8.3, 37C
0.029
-
2-deoxy-CDP-diacylglycerol
-
pH 9.0, 37C
0.045
-
2-deoxy-CDP-diacylglycerol
-
-
0.03
-
CDP-1-stearoyl-2-oleoylglycerol
-
pH 8.3, 37C
0.0095
-
CDP-diacylglycerol
-
pH 8.6, 37C
0.0095
-
CDP-diacylglycerol
-
-
0.01
-
CDP-diacylglycerol
-
pH 8.0, 30C
0.018
-
CDP-diacylglycerol
-
pH 8.5, 37C
0.036
-
CDP-diacylglycerol
-
pH 9.0, 37C
0.036
-
CDP-diacylglycerol
-
-
0.07
-
CDP-diacylglycerol
-
?
0.16
-
CDP-diacylglycerol
-
-
0.18
-
CDP-diacylglycerol
-
pH 8.5, 37C
0.21
-
CDP-diacylglycerol
-
pH 7.5, 37C
0.06
-
CDP-didecanoyl-sn-glycerol
-
-
0.01929
-
CDP-dipalmitin
-
pH 6.8, 37C, 0.010 mM Ca2+
0.0195
-
CDP-dipalmitin
-
pH 6.8, 37C, 0.0001 mM Ca2+
1.35
-
CDP-dipalmitoylglyceride
-
pH 8.5, 37C
0.16
-
CDP-distearoylglycerol
-
pH 8.3, 37C
0.17
-
CDPdiglyceride
-
pH 8.6, 37C
-
0.022
-
CMP
-
pH 8.0, 30C
2.8
-
CMP
-
pH 8.5, 37C
0.05
-
dipalmitoyl-sn-glycerol
-
pH 8.3, 37C
0.015
-
myo-Inositol
-
pH 8.5, 37C, exchange reaction
0.06
-
myo-Inositol
-
pH 7.5, 37C
0.066
-
myo-Inositol
-
pH 8.0, 30C, CMP-independent exchange reaction
0.08
-
myo-Inositol
-
pH 8.0, 30C
0.09
-
myo-Inositol
-
pH 8.0, 30C, forward reaction
0.1
-
myo-Inositol
-
pH 8.5, 37C
0.2
-
myo-Inositol
-
pH 8.5, 37C
0.21
-
myo-Inositol
-
pH 8.0, 37C
0.277
-
myo-Inositol
-
pH 8.0, 30C, CMP-stimulated exchange reaction
0.28
-
myo-Inositol
-
pH 9.0, 37C
0.28
-
myo-Inositol
-
-
0.3
-
myo-Inositol
-
pH 8.5, 37C
0.4
-
myo-Inositol
-
pH 8.0, 30C
0.55
-
myo-Inositol
-
pH 9.0, 30C
0.6
-
myo-Inositol
-
pH 6.8, 37C, 0.01 mM Ca2+
0.76
-
myo-Inositol
-
pH 8.5, 37C
1
-
myo-Inositol
-
-
1.3
-
myo-Inositol
-
pH 8.6, 37C
1.3
-
myo-Inositol
-
-
1.5
-
myo-Inositol
-
pH 6.8, 37C, 0.003 mM Ca2+
1.6
-
myo-Inositol
-
pH 6.8, 37C, 0.0001 mM Ca2+
2.5
-
myo-Inositol
-
pH 8.6, 37C
0.71
-
myo-inositol CDP
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4.1
-
ADP
-
pH 9.0, 37C
0.04
-
AMP
-
pH 8.3, 37C
1.4
-
ATP
-
pH 9.0, 37C
0.6
-
CaCl2
-
pH 9.0, 37C
4
-
CDP
-
pH 9.0, 37C
1.8
-
CTP
-
pH 9.0, 37C
0.2
-
diphosphate
-
pH 9.0, 37C
0.2
-
diphosphate
-
-
1.6
-
GTP
-
pH 9.0, 37C
0.6
-
phosphate
-
pH 9.0, 37C
0.8
-
phosphate
-
-
1.4
-
TTP
-
pH 9.0, 37C
3.8
-
UDP
-
pH 9.0, 37C
1.4
-
UTP
-
pH 9.0, 37C
2
-
ZnCl2
-
pH 9.0, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.000023
-
C0H5B7, -, Q2MJB9
wild-type, in vitro phosphatidylinositol synthesis, yeast membrane extracts after overexpression
0.000036
-
C0H5B7, -, Q2MJB9
hybrid Hy4, in vitro phosphatidylinositol synthesis, yeast membrane extracts after overexpression
0.00025
-
-
-
0.0008
0.0009
-
-
0.001
0.002
-
-
0.002
0.009
-
-
0.0113
-
-
-
additional information
-
C0H5B7, -, Q2MJB9
1.5 nmol/h/1x10(10) cells, wild-type, in culture phosphatidylinositol synthesis, intact yeast cells after overexpression; 2.0 nmol/h/1x10(10) cells, hybrid Hy4, in culture phosphatidylinositol synthesis, intact yeast cells after overexpression; 3.4 nmol/min per 1x10(10) infected red blood cells; 3.7 nmol/h/1x10(10) cells, hybrid Hy3, in culture phosphatidylinositol synthesis, intact yeast cells after overexpression; no detectable activity for hybrid Hy3, in vitro phosphatidylinositol synthesis, yeast membrane extracts after overexpression
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.2
-
-
pH optimum for reverse reaction
6.5
7
-
pH optimum in reverse direction
7
-
-
optimum for exchange reaction
7.7
-
-
CTP and phosphatidic acid as substrate
7.8
-
-
; 30C
8
-
-
in Tris/HCl buffer, maximal activity is 60% of the activity in glycylglycine/NaOH at pH 9.0, at pH 9.0 almost no activity in Tris/HCl buffer
8
-
-
highest activity in Tris buffer, only 43% of the activity in glycine/NaOH buffer at pH 9.5
8
-
-
exchange reaction
8.5
9
-
CDP-diacyl-sn-glycerol as substrate
8.5
9
-
optimum in the forward direction
8.5
9
-
-
8.5
-
-
pH optimum for reverse reaction
9
-
-
in glycylglycine/NaOH or glycine/NaOH buffer
9.5
-
-
glycine NaOH buffer
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
10
-
little or no activity detectable at pH 6.3 and pH 5.5
6.5
10.5
-
-
6.5
9.5
-
at pH 7.0 activity is approximately one-fourth that observed at optimum pH 8.8, enzyme is equally active at pH 8.5
7.3
8.4
-
30C, 90% of maximum activity; about 90% of maximum activity within this range
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
; pH 7.8
30
-
-
assay at
50
-
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
exchange reaction exhibits reduced activity above
50
-
-
89% of maximum activity, pH 7.8, 10 min preincubation
60
-
-
82% of maximum activity, pH 7.8, 10 min preincubation
70
-
-
79% of maximum activity, pH 7.8, 10 min preincubation
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
localized to both the rough and the smooth endoplasmic reticulum
Manually annotated by BRENDA team
Q8GUK6, Q8LBA6
transient gene expression in onion epidermal cells, C-terminal CFP-tag, confocal microscopy, centre of punctuate structures; transient gene expression in onion epidermal cells, C-terminal EYFP-tag, confocal microscopy, periphery of punctuate structures
Manually annotated by BRENDA team
Q8GUK6, Q8LBA6
transient gene expression in onion epidermal cells, C-terminal EYFP-tag, confocal microscopy, limited overlap with marker (rat sialyl-S-transferase); transient gene expression in onion epidermal cells, C-terminal EYFP-tag, confocal microscopy, limited overlap with marker (rat sialyl-S-transferase)
Manually annotated by BRENDA team
Q8GUK6, Q8LBA6
membrane fraction of overexpressing Escherichia coli; membrane fraction of overexpressing Escherichia coli
Manually annotated by BRENDA team
-
; microsomal fractions from Tetrahymena vorax, positive for NADPH-cytochrome c reductase activity
-
Manually annotated by BRENDA team
Tetrahymena vorax V2S
-
; microsomal fractions from Tetrahymena vorax, positive for NADPH-cytochrome c reductase activity
-
-
Manually annotated by BRENDA team
-
sub-cellular localization of PIS is studied in Solanum tuberosum by membrane fractionation, enzymatic analysis and by confocal microscopy in living cells. In potato, beside the endoplasmic reticulum, the activity of PIS is found to be tightly associated to mitochondria, more specifically to the outer mitochondrial membrane
Manually annotated by BRENDA team
additional information
-
little or no activity in the plastids
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
23610
-
-
primary translate deduced from the encoding gene, PIS
23740
-
-, Q2MJB8
theoretical
23920
-
C0H5B7, -, Q2MJB9
theoretical
24000
-
-
SDS-PAGE
24000
-
-
calculated from cDNA
24820
-
-
calculated molecular mass from the cloned PI synthase gene
27800
-
Q9XY91, -
calculated from cDNA
34000
-
-
SDS-PAGE
34000
-
-
SDS-PAGE
60000
-
-
SDS-PAGE
150000
200000
-
gel filtration
300000
-
-
partially purified enzyme, ultrafiltration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
? * 34000, SDS-PAGE
?
-
? * 30000 + ? * 60000, SDS-PAGE
?
-
? * 21000, SDS-PAGE
?
C0H5B7, -, Q2MJB9
x * 23924, calculated
?
-, Q2MJB8
x * 23735, calculated
monomer
-
SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
bound to the cofactor NAD+, space group P42(1)2, cell dimensions a = 116.2, b = 116.2, c = 64.5
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
thermally inactivated at temperatures above, thermally unstable when incubated for 20 min, negligible activity present at 45C
30
-
-
exchange reaction exhibits reduced activity above
40
50
-
thermal denaturation at higher temperatures, half-life at 50C 30 min
50
-
-
half-life without substrates 20 min
60
-
-
reasonably stable up to, essentially 100% stable for at least 10 min at temperatures ranging from 5 to 50C, inactivation of about 35% at 60C and 100% at 70C
60
-
-
labile above
70
-
-
10 min, 80% residual activity
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
addition of bovine serum albumin is necessary in assaying the enzyme
-
stable to at least 2 cycles of freezing and thawing with no loss in enzyme activity
-
inactivation caused by repeated freezing and thawing or by contact with detergent is prevented by addition of dithiothreitol and CDPdiacyl-sn-glycerol
-
loses activity when the lipids are removed
-
activity is stable for at least 4 cycles of freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, frozen in liquid N2 and stored, no loss of activity is detected during storage for up to 2 months
-
-80C, no appreciable loss of PI synthase activity detected for up to at least 6 months
-
4C, 67% of the activity remains after 3 days
-
-80C, glycerol stabilizes PI synthase to storage, stable to storage for at least 3 months
-
-20C, frozen and stored or frozen in liquid nitrogen and subsequently stored, over 50% activity is lost after 24 h
-
-60C, frozen and stored or frozen in liquid nitrogen and subsequently stored, over 50% activity is lost after 24 h
-
-80C, regularly frozen in liqid N2, no detectable loss of activity is experienced during storage at -80C for up to 2 months
-
4C, stored on ice, 10% of the activity is lost during 24 h
-
-70C, substantial loss of enzymic activity when stored for more than 48 h
-
-20C, substrate and dithioerythritol protects the enzyme activity for several days
-
-80C, can be stored for a few days without appreciable loss of activity, inactivated gradually during storage for longer periods
-
-80C, partially purified enzyme can be stored for at least 3 months without loss of activity
-
-80C, purified enzyme is completely stable for at least 6 months
-
8C, enzyme is 100% stabe for at least 3 days of storage
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
as bacterial membrane pellets, no further specific purification possible; as bacterial membrane pellets, no further specific purification possible
Q8GUK6, Q8LBA6
recombinant enzyme
-
partially
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
; AtPIS1 cDNA cloned in Escherichia coli XL-1 Blue, functional complementation of Saccharomyces cerevisiae wild-type strain D501-1
-, Q8GUK6
; AtPIS1 cloned and expressed in Escherichia coli, a host naturally devoid of phosphatidylinositol; cloned and expressed in Escherichia coli
-
; gene AtPIS1 cDNA cloned and expressed in Escherichia coli
-
from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP for introduction of C-terminal EYFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections; from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP or -CFP for introduction of C-terminal EYFP- or CFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections
Q8GUK6, Q8LBA6
-
-, Q8LKS7
yeast cDNA S45885 used to search, pis1 cDNA cloned and expressed in COS-7 cells
O14735
tbINO, the coding sequence of ino1 amplified by PCR, cloned into expression vector pET-15b and overproduced in Escherichia coli BL21 (DE3)
-
; highly spliced gene with 9 exons, cDNA encodes 209 amino acids, from total RNA in pCR-BluntII-Topo for cloning and sequencing (transformation of Escherichia coli TOP10 and DH5alpha), in p413 for complementation studies in Saccharomyces cerevisiae
C0H5B7, -, Q2MJB9
; highly spliced gene with 9 exons, cDNA encodes 207 amino acids, from mRNA in pCRII-Blunt-TOPO for cloning and sequencing (transformation of Escherichia coli TOP10 and DH5alpha)
-, Q2MJB8
cDNA cloned by functional complementation of a Saccharomyces cerevisiae mutant
-
rat PIS expressed in mouse NIH3T3 fibroblasts by transfection with a FLAG-tagged PIS cDNA using the vector pcDNA3
-
disruption of the PIS locus in the genome is lethal
-
gene PIS cloned with pUC8 and expressed in Escherichia coli HB101
-
; cDNA cloned by functional complementation in Saccharomyces cerevisiae
Q9XY91, -
expressed in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C148W
Q8GUK6, Q8LBA6
polymorphism of unclear relevance, amplified from plant cDNA, mismatch with annotated genomic sequence
S111C
-
missense mutation leads to lens opaque (lop) phenotype. Mutation is located in the catalytic domain of the 213 amino acid phosphatidylinositol (PI) synthase enzyme
Hy3
C0H5B7, -, Q2MJB9
hybrid construct of 57 N-terminal amino acids from Saccharomyces cerevisiae PIS and 159 C-terminal amino acids from PfPIS, no in vitro activity, 60% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%)
Hy4
C0H5B7, -, Q2MJB9
hybrid construct of 14 N-terminal amino acids from Saccharomyces cerevisiae PIS and 202 C-terminal amino acids from PfPIS, 37% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%)
additional information
C0H5B7, -, Q2MJB9
gene successfully complements a Saccharomyces cerevisiae PIS1 deletion mutant
H114Q
-
oligonucleotide-mediated site-directed mutagenesis, substitution of amino acid at position 114 from His (CAC) + Gln (CAA) results in a 200fold increase in Km of the enzyme for myo-inositol, making cells auxotrophic for myo-inositol
additional information
Q6KBS8
conditional double knockout mutant in the bloodstream form of Trypanosoma is not viable under nonpermissive conditions, which lead to a decrease in the amount of enzyme protein
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
one of the major human fungal pathogens, expanded knowledge about essential metabloic processes and enzymes in this pathogenic fungus can pave the way for the development of more effective treatments
medicine
-
enzymes involved in the PI biosynthetic pathway are potential antimycobacterial agents
medicine
Q9XY91, -
causative agent of toxoplasmosis, screening of drugs against the parasite enzyme
medicine
Toxoplasma gondii 76K
-
causative agent of toxoplasmosis, screening of drugs against the parasite enzyme
-