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Information on EC 2.7.7.75 - molybdopterin adenylyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q39054

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EC Tree
IUBMB Comments
Catalyses the activation of molybdopterin for molybdenum insertion. In eukaryotes, this reaction is catalysed by the C-terminal domain of a fusion protein that also includes molybdopterin molybdotransferase (EC 2.10.1.1). The reaction requires a divalent cation such as Mg2+ or Mn2+.
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Arabidopsis thaliana
UNIPROT: Q39054
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
aamoga, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cnx1
C-terminal G-domain, gene name
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
ATP:molybdopterin adenylyltransferase
Catalyses the activation of molybdopterin for molybdenum insertion. In eukaryotes, this reaction is catalysed by the C-terminal domain of a fusion protein that also includes molybdopterin molybdotransferase (EC 2.10.1.1). The reaction requires a divalent cation such as Mg2+ or Mn2+.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + molybdopterin
diphosphate + adenylyl-molybdopterin
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + molybdopterin
diphosphate + adenylyl-molybdopterin
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diphosphate
efficient inhibition at 1-10 mM diphosphate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diphosphate
activating by 5 units in the standard reaction set-up
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.062
ATP
10 mM MgCl2, pH 7.2, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0012
ATP
10 mM MgCl2, pH 7.2, temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
domain G; commentary
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
molybdenum cofactor biosynthesis
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CNX1_ARATH
670
0
71279
Swiss-Prot
other Location (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D515H
D515N
in Cnx1 G-domain, accumulation of molybdopterin
N597L
in Cnx1 G-domain, strong decrease in molybdopterin binding
R547E
decreased activity
S573A
inactive in absence of pyrophosphatase, decreased actitivity in presence of pyrophosphatase
S583A
increased activity
T542A
decreased activity
T542S
decreased activity
V557G
in Cnx1 G-domain, strong decrease in molybdopterin binding
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-nitrilotriacetic acid matrix
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Cnx1 G-domain in pQE60
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Llamas, A.; Mendel, R.R.; Schwarz, G.
Synthesis of adenylated molybdopterin: an essential step for molybdenum insertion
J. Biol. Chem.
279
55241-55246
2004
Arabidopsis thaliana (Q39054)
Manually annotated by BRENDA team
Kuper, J.; Palmer, T.; Mendel, R.R.; Schwarz, G.
Mutations in the molybdenum cofactor biosynthetic protein Cnx1G from Arabidopsis thaliana define functions for molybdopterin binding, molybdenum insertion, and molybdenum cofactor stabilization
Proc. Natl. Acad. Sci. USA
97
6475-6480
2000
Arabidopsis thaliana (Q39054)
Manually annotated by BRENDA team