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CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
additional information
?
-
-
lipoate-protein ligase attaches octanoate to the dehydrogenase subunit and sulfur insertion protein LipA then converts octanoate to lipoate. LipA acts on both octanoate and octanoyl-proteins
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
r
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
essential enzyme in he mevalonate-independent pathway of isoprenoid biosynthesis
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis
-
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
intermediate in the deoxyxylulose pathway of isoprenoid biosynthesis
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
third step of biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
third step of biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
part of mevalonate-indpendent pathway for isoprenoid biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
part of mevalonate-indpendent pathway for isoprenoid biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
part of mevalonate-indpendent pathway for isoprenoid biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
part of mevalonate-indpendent pathway for isoprenoid biosynthesis
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis
-
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
essential enzyme in he mevalonate-independent pathway of isoprenoid biosynthesis
-
-
?
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis
-
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
-
intermediate in the deoxyxylulose pathway of isoprenoid biosynthesis
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
third step of biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
third step of biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
part of mevalonate-indpendent pathway for isoprenoid biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
part of mevalonate-indpendent pathway for isoprenoid biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
part of mevalonate-indpendent pathway for isoprenoid biosynthesis
-
?
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
part of mevalonate-indpendent pathway for isoprenoid biosynthesis
-
?
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0.2915 - 131.5
2-C-methyl-D-erythritol 4-phosphate
0.131
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
0.061
2-C-methyl-D-erythritol 4-phosphate
-
pH 7.5, 30°C
0.00314 - 0.032
2-C-methyl-D-erythritol-4-phosphate
additional information
additional information
-
0.2915
2-C-methyl-D-erythritol 4-phosphate
recombinant detagged enzyme, pH 8.0, 30°C
0.31
2-C-methyl-D-erythritol 4-phosphate
R20K mutant enzyme, pH 6.5, 23°C
0.37
2-C-methyl-D-erythritol 4-phosphate
wild type enzyme, pH 6.5, 23°C
0.74
2-C-methyl-D-erythritol 4-phosphate
R19K mutant enzyme, pH 6.5, 23°C
2.98
2-C-methyl-D-erythritol 4-phosphate
R19A mutant enzyme, pH 6.5, 23°C
3
2-C-methyl-D-erythritol 4-phosphate
K27S mutant enzyme, pH 6.5, 23°C
3.28
2-C-methyl-D-erythritol 4-phosphate
R20A mutant enzyme, pH 6.5, 23°C
15.5
2-C-methyl-D-erythritol 4-phosphate
D106E mutant enzyme, pH 6.5, 23°C
49
2-C-methyl-D-erythritol 4-phosphate
T140V mutant enzyme, pH 6.5, 23°C
131.5
2-C-methyl-D-erythritol 4-phosphate
R109A mutant enzyme, pH 6.5, 23°C
0.003
CTP
-
0.2309
CTP
recombinant detagged enzyme, pH 8.0, 30°C
0.76
CTP
wild type enzyme, pH 6.5, 23°C
0.85
CTP
R19K mutant enzyme, pH 6.5, 23°C
1.1
CTP
K27S mutant enzyme, pH 6.5, 23°C
1.4
CTP
D106E mutant enzyme, pH 6.5, 23°C
2
CTP
T140V mutant enzyme, pH 6.5, 23°C
2.8
CTP
R19A mutant enzyme, pH 6.5, 23°C
3
CTP
R109A mutant enzyme, pH 6.5, 23°C
3.7
CTP
R20K mutant enzyme, pH 6.5, 23°C
6.6
CTP
R20A mutant enzyme, pH 6.5, 23°C
0.00314
2-C-methyl-D-erythritol-4-phosphate
-
-
0.032
2-C-methyl-D-erythritol-4-phosphate
-
-
0.061
CTP
-
pH 7.5, 30°C
additional information
additional information
kinetic analysis
-
additional information
additional information
-
kinetic analysis
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.4 - 52.7
2-C-methyl-D-erythritol 4-phosphate
16.8
2-C-methyl-D-erythritol-4-phosphate
-
-
0.4
2-C-methyl-D-erythritol 4-phosphate
R109A mutant enzyme, pH 6.5, 23°C
0.75
2-C-methyl-D-erythritol 4-phosphate
K27S mutant enzyme, pH 6.5, 23°C
0.9
2-C-methyl-D-erythritol 4-phosphate
D106E mutant enzyme, pH 6.5, 23°C
4
2-C-methyl-D-erythritol 4-phosphate
T140V mutant enzyme, pH 6.5, 23°C
21.4
2-C-methyl-D-erythritol 4-phosphate
R20K mutant enzyme, pH 6.5, 23°C
36.8
2-C-methyl-D-erythritol 4-phosphate
R19A mutant enzyme, pH 6.5, 23°C
38.45
2-C-methyl-D-erythritol 4-phosphate
recombinant detagged enzyme, pH 8.0, 30°C
47.2
2-C-methyl-D-erythritol 4-phosphate
R19K mutant enzyme, pH 6.5, 23°C
48.4
2-C-methyl-D-erythritol 4-phosphate
wild type enzyme, pH 6.5, 23°C
52.7
2-C-methyl-D-erythritol 4-phosphate
R20A mutant enzyme, pH 6.5, 23°C
0.26
CTP
R109A mutant enzyme, pH 6.5, 23°C
0.93
CTP
D106E mutant enzyme, pH 6.5, 23°C
1.8
CTP
T140V mutant enzyme, pH 6.5, 23°C
2.05
CTP
K27S mutant enzyme, pH 6.5, 23°C
27.5
CTP
R19A mutant enzyme, pH 6.5, 23°C
40.2
CTP
R20A mutant enzyme, pH 6.5, 23°C
46.4
CTP
R19K mutant enzyme, pH 6.5, 23°C
53.6
CTP
R20K mutant enzyme, pH 6.5, 23°C
54.1
CTP
wild type enzyme, pH 6.5, 23°C
79.5
CTP
recombinant detagged enzyme, pH 8.0, 30°C
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Cane, D.E.; Chow, C.; Lillo, A.; Kang, I.
Molecular cloning, expression and characterization of the first three genes in the mevalonate-independent isoprenoid pathway in Streptomyces coelicolor
Bioorg. Med. Chem.
9
1467-1477
2001
Escherichia coli, Streptomyces coelicolor, Streptomyces coelicolor CH999
brenda
Richard, S.B.; Bowman, M.E.; Kwiatkowski, W.; Kang, L.; Chow, C.; Lillo, A.M.; Cane, D.E.; Noel, J.P.
Structure of 4-diphosphocytidyl-2-C-methylerythritol synthetase involved in mevalonate-independent isoprenoid biosynthesis
Nature Struct. Biol.
8
641-648
2001
Escherichia coli, Mycobacterium tuberculosis, Plasmodium falciparum
brenda
Kemp, L.E.; Bond, C.S.; Hunter, W.N.
Crystallization and preliminary x-ray diffraction studies of recombinant Escherichia coli 4-diphosphocytidyl-2-C-methyl-D-erythritol synthetase
Acta Crystallogr. Sect. D
57
1189-1191
2001
Escherichia coli
brenda
Rohdich, F.; Wungsintaweekul, J.; Eisenreich, W.; Richter, G.; Schuhr, C.A.; Hecht, S.; Zenk, M.H.; Bacher, A.
Biosynthesis of terpenoids: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase of Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
97
6451-6456
2000
Arabidopsis thaliana, Escherichia coli (Q46893), Escherichia coli
brenda
Kuzuyama, T.; Takagi, M.; Kaneda, K.; Dairi, T.; Seto, H.
Formation of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from 2-C-methyl-D-erythritol 4-phosphate by 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, a new enzyme in the nonmevalonate pathway
Tetrahedron Lett.
41
703-706
2000
Escherichia coli
-
brenda
Rohdich, F.; Wungsintaweekul, J.; Fellermeier, M.; Sagner, S.; Herz, S.; Kis, K.; Eisenreich, W.; Bacher, A.; Zenk, M.H.
Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol
Proc. Natl. Acad. Sci. USA
96
11758-11763
1999
Arabidopsis thaliana, Escherichia coli, Escherichia coli DH5-alpha
brenda
Gabrielsen, M.; Bond, C.S.; Hallyburton, I.; Hecht, S.; Bacher, A.; Eisenreich, W.; Rohdich, F.; Hunter, W.N.
Hexameric assembly of the bifunctional methylerythritol 2,4-cyclodiphosphate synthase and protein-protein associations in the deoxy-xylulose-dependent pathway of isoprenoid precursor biosynthesis
J. Biol. Chem.
279
52753-52761
2004
Agrobacterium tumefaciens, Campylobacter jejuni, Escherichia coli (Q46893), Escherichia coli
brenda
Kemp, L.E.; Bond, C.S.; Hunter, W.N.
Structure of a tetragonal crystal form of Escherichia coli 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Acta Crystallogr. Sect. D
59
607-610
2003
Escherichia coli
brenda
Bernal, C.; Palacin, C.; Boronat, A.; Imperial, S.
A colorimetric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol 4-phosphate synthase activity
Anal. Biochem.
337
55-61
2005
Escherichia coli
brenda
Richard, S.B.; Lillo, A.M.; Tetzlaff, C.N.; Bowman, M.E.; Noel, J.P.; Cane, D.E.
Kinetic analysis of Escherichia coli 2-C-methyl-D-erythritol-4-phosphate cytidyltransferase, wild type and mutants, reveals roles of active site amino acids
Biochemistry
43
12189-12197
2004
Escherichia coli (Q46893), Escherichia coli
brenda
Zhao, X.; Miller, J.R.; Jiang, Y.; Marletta, M.A.; Cronan, J.E.
Assembly of the covalent linkage between lipoic acid and its cognate enzymes
Chem. Biol.
10
1293-1302
2003
Escherichia coli
brenda
Illarionova, V.; Kaiser, J.; Ostrozhenkova, E.; Bacher, A.; Fischer, M.; Eisenreich, W.; Rohdich, F.
Nonmevalonate terpene biosynthesis enzymes as antiinfective drug targets: substrate synthesis and high-throughput screening methods
J. Org. Chem.
71
8824-8834
2006
Escherichia coli
brenda
Haymond, A.; Dowdy, T.; Johny, C.; Johnson, C.; Ball, H.; Dailey, A.; Schweibenz, B.; Villarroel, K.; Young, R.; Mantooth, C.J.; Patel, T.; Bases, J.; Dowd, C.S.; Couch, R.D.
A high-throughput screening campaign to identify inhibitors of DXP reductoisomerase (IspC) and MEP cytidylyltransferase (IspD)
Anal. Biochem.
542
63-75
2018
Escherichia coli (Q46893), Francisella tularensis subsp. novicida (A0Q5K1), Francisella tularensis subsp. novicida Utah 112 (A0Q5K1)
brenda
Jin, Y.; Liu, Z.; Li, Y.; Liu, W.; Tao, Y.; Wang, G.
A structural and functional study on the 2-C-methyl-D-erythritol-4-phosphate cytidyltransferase (IspD) from Bacillus subtilis
Sci. Rep.
6
36379
2016
Bacillus subtilis (Q06755), Bacillus subtilis, Escherichia coli (Q46893), Escherichia coli, Bacillus subtilis 168 (Q06755), Bacillus subtilis DSM 23778 (Q06755)
brenda