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Information on EC 2.7.7.59 - [protein-PII] uridylyltransferase and Organism(s) Azospirillum brasilense and UniProt Accession Q8RQD1

for references in articles please use BRENDA:EC2.7.7.59
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IUBMB Comments
The enzyme uridylylates and de-uridylylates the small trimeric protein PII. The enzymes from Escherichia coli and Salmonella typhimurium have been wrongly identified, in some databases, as EC 2.7.7.12 (UDP-glucose---hexose-1-phosphate uridylyltransferase), from which it differs greatly in both reaction catalysed and sequence.
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This record set is specific for:
Azospirillum brasilense
UNIPROT: Q8RQD1
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Word Map
The taxonomic range for the selected organisms is: Azospirillum brasilense
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
uridylyltransferase, uridylyl transferase, utase/ur, uridylyltransferase/uridylyl-removing enzyme, utase, glnd protein, uridylyl-removing enzyme, uridylyl-removing activity, pii uridylyltransferase, uridylyltransferase enzyme, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PII uridylyltransferase
UniProt
uridylyl-removing enzyme
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uridylyltransferase
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[protein-PII]-UMP uridylyl-removing enzyme
UR, UniProt
PII uridylyl-transferase
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-
-
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PII uridylyltransferase
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-
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PII uridylyltransferase (Escherichia coli gene glnD)
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-
-
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uridyl removing enzyme
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-
-
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uridyltransferase, glutamine synthetase adenylyltransferase-regulating protein
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-
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uridylyl removing enzyme
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-
-
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uridylyltransferase, glutamine synthetase adenylyltransferase-regulating protein (Escherichia coli clone 21C8 gene glnD reduced)
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-
-
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uridylyltransferase/uridylyl removing enzyme
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uridylyltransferase/uridylyl-removing enzyme
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UTase/UR
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-
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UTP:PII protein uridylyltransferase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
UTP:[protein-PII] uridylyltransferase
The enzyme uridylylates and de-uridylylates the small trimeric protein PII. The enzymes from Escherichia coli and Salmonella typhimurium have been wrongly identified, in some databases, as EC 2.7.7.12 (UDP-glucose---hexose-1-phosphate uridylyltransferase), from which it differs greatly in both reaction catalysed and sequence.
CAS REGISTRY NUMBER
COMMENTARY hide
151596-34-8
Escherichia coli clone 21C8 gene glnD reduced
57657-57-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diphosphate + uridylyl-[protein-PII GlnB]
UTP + [protein-PIIGlnB]
show the reaction diagram
-
-
-
r
diphosphate + uridylyl-[protein-PII GlnZ]
UTP + [protein-PIIGlnZ]
show the reaction diagram
-
-
-
r
UTP + GlnB
diphosphate + uridylyl-GlnB
show the reaction diagram
-
-
-
r
UTP + GlnZ
diphosphate + uridylyl-GlnZ
show the reaction diagram
-
-
-
r
UTP + [protein-PII GlnB]
diphosphate + uridylyl-[protein-PII GlnB]
show the reaction diagram
PII-1 protein
-
-
r
UTP + [protein-PII GlnZ]
diphosphate + uridylyl-[protein-PII GlnZ]
show the reaction diagram
PII-2 protein
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diphosphate + uridylyl-[protein-PII GlnB]
UTP + [protein-PIIGlnB]
show the reaction diagram
-
-
-
r
diphosphate + uridylyl-[protein-PII GlnZ]
UTP + [protein-PIIGlnZ]
show the reaction diagram
-
-
-
r
UTP + GlnB
diphosphate + uridylyl-GlnB
show the reaction diagram
-
-
-
r
UTP + GlnZ
diphosphate + uridylyl-GlnZ
show the reaction diagram
-
-
-
r
UTP + [protein-PII GlnB]
diphosphate + uridylyl-[protein-PII GlnB]
show the reaction diagram
PII-1 protein
-
-
r
UTP + [protein-PII GlnZ]
diphosphate + uridylyl-[protein-PII GlnZ]
show the reaction diagram
PII-2 protein
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-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NH4+
uridylylation in response to the concentration of ammonium ions
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
Azospirillum brasilense is a diazotrophic microorganism capable of associating with roots of important grasses and cereals, promoting plant growth and increasing crop yields. Nitrogen levels and the Ntr regulatory system control the nitrogen metabolism in Azospirillum brasilense. This system comprises the nitrogen regulatory proteins GlnD, which is capable of adding uridylyl groups to the PII proteins, GlnB (PII-1) and GlnZ (PII-2), under limiting nitrogen levels. Under such conditions, the histidine kinase NtrB (nitrogen regulatory protein B) cannot interact with GlnB and phosphorylate NtrC (nitrogen regulatory protein C). The phosphorylated form of NtrC acts as a transcriptional activator of genes involved in the metabolism of alternative nitrogen sources. Analysis of proteomic profile and proteins regulated by nitrogen availability in Azospirillum brasilense ntrC, identification of proteins likely to belong to the NtrC regulon, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GLND_AZOBR
933
0
104602
Swiss-Prot
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
on a Hi-trap-chelating-Ni2+ column
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene glnD, gene expression evaluation by RT-PCR
into the vector pET28a for expression in Escherichia coli cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Araujo, L.M.; Huergo, L.F.; Invitti, A.L.; Gimenes, C.I.; Bonatto, A.C.; Monteiro, R.A.; Souza, E.M.; Pedrosa, F.O.; Chubatsu, L.S.
Different responses of the GlnB and GlnZ proteins upon in vitro uridylylation by the Azospirillum brasilense GlnD protein
Braz. J. Med. Biol. Res.
41
289-294
2008
Azospirillum brasilense (Q8RQD1), Azospirillum brasilense
Manually annotated by BRENDA team
Kukolj, C.; Pedrosa, F.O.; de Souza, G.A.; Sumner, L.W.; Lei, Z.; Sumner, B.; do Amaral, F.P.; Juexin, W.; Trupti, J.; Huergo, L.F.; Monteiro, R.A.; Valdameri, G.; Stacey, G.; de Souza, E.M.
Proteomic and metabolomic analysis of Azospirillum brasilense ntrC mutant under high and low nitrogen conditions
J. Proteome Res.
19
92-105
2020
Azospirillum brasilense (Q8RQD1), Azospirillum brasilense FP2 (Q8RQD1)
Manually annotated by BRENDA team