Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.7.49 - RNA-directed DNA polymerase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P0CX63

for references in articles please use BRENDA:EC2.7.7.49
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Catalyses RNA-template-directed extension of the 3'- end of a DNA strand by one deoxynucleotide at a time. Cannot initiate a chain de novo. Requires an RNA or DNA primer. DNA can also serve as template. See also EC 2.7.7.7 DNA-directed DNA polymerase.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P0CX63 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Synonyms
telomerase, reverse-transcriptase, telomerase reverse transcriptase, nucleoside reverse transcriptase, hiv-1 rt, hiv-1 reverse transcriptase, gag-pol, hiv reverse transcriptase, rna-dependent dna polymerase, telomerase catalytic subunit, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DNA nucleotidyltransferase (RNA-directed)
-
-
-
-
MX162-RT
-
-
-
-
MX65-RT
-
-
-
-
nucleotidyltransferase, deoxyribonucleate, RNA-dependent
-
-
-
-
P72
-
-
-
-
reverse transcriptase
revertase
-
-
-
-
RNA revertase
-
-
-
-
RNA-dependent DNA polymerase
-
-
-
-
RNA-instructed DNA polymerase
-
-
-
-
RT
-
-
-
-
telomerase catalytic subunit
-
-
-
-
telomerase reverse transcriptase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (RNA-directed)
Catalyses RNA-template-directed extension of the 3'- end of a DNA strand by one deoxynucleotide at a time. Cannot initiate a chain de novo. Requires an RNA or DNA primer. DNA can also serve as template. See also EC 2.7.7.7 DNA-directed DNA polymerase.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-38-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
additional information
?
-
-
elongates telomeres to tolerate mutations in the telomeric template
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
the enzyme plays a central role in transposition of retroelements
-
-
?
additional information
?
-
-
elongates telomeres to tolerate mutations in the telomeric template
-
-
?
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.16
p-tRNAHis
-
pH 7.5, 22°C
-
4.36 - 5.58
ppp-tRNAHis
-
0.039
ppp-tRNALeu
-
pH 7.5, 22°C, mutant enzyme D68A
-
0.0048 - 0.42
ppp-tRNAPhe
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TY1AB_YEASX
1755
0
198662
Swiss-Prot
other Location (Reliability: 3)
E9P8M7_YEASX
876
0
101734
TrEMBL
other Location (Reliability: 1)
E9P8M4_YEASX
872
0
101153
TrEMBL
other Location (Reliability: 1)
A0A8H4BZU1_YEASX
882
0
102391
TrEMBL
other Location (Reliability: 2)
E9P8M1_YEASX
880
0
102204
TrEMBL
other Location (Reliability: 2)
E9P8M3_YEASX
880
0
102107
TrEMBL
other Location (Reliability: 1)
E9P8M8_YEASX
873
0
101295
TrEMBL
other Location (Reliability: 1)
E9P8N0_YEASX
873
0
101268
TrEMBL
other Location (Reliability: 1)
Q0P7C9_YEASX
876
0
101689
TrEMBL
other Location (Reliability: 1)
E9P8M6_YEASX
876
0
101718
TrEMBL
other Location (Reliability: 1)
E9P8M2_YEASX
874
0
101442
TrEMBL
other Location (Reliability: 1)
E9P8M5_YEASX
873
0
101249
TrEMBL
other Location (Reliability: 1)
E9P8M9_YEASX
878
0
101913
TrEMBL
other Location (Reliability: 1)
E9P8N1_YEASX
876
0
101570
TrEMBL
other Location (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D211N
-
still capable of in vitro polymerization, although it is blocked for in vivo transposition. D211N mutation has minimal effect on nucleotide binding but reduces the kpol by about 230fold. The mutation reduces binding affinity for both Mn2+ and Mg2+
F292A
-
slight diminuation in DNA-dependent DNA synthesis (under conditions allowing multiple rounds of synthesis). Significant reduction in polymerase activity in presence of heparin
F292Y
-
severly impaired Rnase H activity
G294A
-
mutation results in enhanced pausing at multiple positions of the DNA template in DNA-dependent DNA synthesis (under conditions allowing multiple rounds of synthesis). Significant reduction in polymerase activity in presence of heparin
Y298A
-
slight diminuation in DNA-dependent DNA synthesis (under conditions allowing multiple rounds of synthesis). Significant reduction in polymerase activity in presence of heparin
Y298W
-
significant reduction in polymerase activity in presence of heparin
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wilhelm, F.X.; Wilhelm, M.; Gabriel, A.
Reverse transcriptase and integrase of the Saccharomyces cerevisiae Ty1 element
Cytogenet. Genome Res.
110
269-287
2005
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Pandey, M.; Patel, S.; Gabriel, A.
Insights into the role of an active site aspartate in Ty1 reverse transcriptase polymerization
J. Biol. Chem.
279
47840-47848
2004
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Bibillo, A.; Lener, D.; Tewari, A.; Le Grice, S.F.
Interaction of the Ty3 reverse transcriptase thumb subdomain with template-primer
J. Biol. Chem.
280
30282-30290
2005
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Gipson, C.L.; Xin, Z.T.; Danzy, S.C.; Parslow, T.G.; Ly, H.
Functional characterization of yeast telomerase RNA dimerization
J. Biol. Chem.
282
18857-18863
2007
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Auriche, C.; Di Domenico, E.G.; Ascenzioni, F.
Budding yeast with human telomeres: a puzzling structure
Biochimie
90
108-115
2008
Saccharomyces cerevisiae
Manually annotated by BRENDA team