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Information on EC 2.7.7.41 - phosphatidate cytidylyltransferase and Organism(s) Rattus norvegicus and UniProt Accession O35052

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Rattus norvegicus
UNIPROT: O35052 not found.
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
cdp-diacylglycerol synthase, cdp-dg, phosphatidate cytidylyltransferase, tam41, cdp-dag synthase, tamm41, ctp:phosphatidate cytidylyltransferase, cdp-diglyceride synthetase, cdp-dg synthase, tbcds, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CDP diacylglycerol synthase
-
CDP-DAG synthase
-
-
-
-
CDP-DG
-
-
-
-
CDP-DG synthetase
-
-
-
-
CDP-diacylglyceride synthetase
-
-
-
-
CDP-diacylglycerol synthase
-
-
-
-
CDP-diglyceride pyrophosphorylase
-
-
-
-
CDP-diglyceride synthetase
-
-
-
-
CDPdiglyceride pyrophosphorylase
-
-
-
-
CDS
-
-
-
-
CTP-diacylglycerol synthetase
-
-
-
-
CTP:1,2-diacylglycerophosphate-cytidyl transferase
-
-
-
-
CTP:phosphatidate cytidylyltransferase
-
-
-
-
cytidine diphosphoglyceride pyrophosphorylase
-
-
-
-
cytidylyltransferase, phosphatidate
-
-
-
-
DAG synthetase
-
-
-
-
phosphatidate cytidyltransferase
-
-
-
-
phosphatidic acid cytidylyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
CTP:phosphatidate cytidylyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9067-83-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + 1,2-dioleoyl-sn-glycerol 3-phosphate
diphosphate + CDP-1,2-dioleoylglycerol
show the reaction diagram
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
show the reaction diagram
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
the enzyme prefers 1-stearoyl-2-arachidonoyl phosphatidic acid as substrate. Little or no activity is detected towards phosphatidic acids containing saturated fatty acyl groups in both the sn-1 and sn-2 positions
-
-
?
CTP + 1,2-diarachidonoyl phosphatidic acid
diphosphate + CDP-1,2-diarachidonoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1,2-dicaproyl phosphatidic acid
diphosphate + CDP-1,2-dicaproylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1,2-dioleoyl phosphatidic acid
diphosphate + CDP-dioleoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1,2-dipalmitoyl phosphatidic acid
diphosphate + CDP-dipalmitoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1,2-distearoyl phosphatidic acid
diphosphate + CDP-1,2-distearoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-arachidonoyl-2-stearoyl phosphatidic acid
diphosphate + CDP-1-arachidonoyl-2-stearoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-oleoyl-2-stearoyl phosphatidic acid
diphosphate + CDP-1-oleoyl-2-stearoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-palmitoyl-2-oleoyl phosphatidic acid
diphosphate + CDP-1-palmitoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-stearoyl-2-oleoyl phosphatidic acid
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphatidylinositol 4,5-bisphosphate
strong
ATP
-
3 mM 50% inhibition. 5 mM, complete inhibition
CDP-diacylglycerol
-
-
F-
-
20 mM, completely abolishes the stimulatory effect of GTP
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
-
activity is 6fold increased
-
Norfenfluramine
-
can partially replace the divalent cation requirement
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.114
1,2-dioleoyl-sn-glycerol 3-phosphate
pH 7.5, 30°C
0.102
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphate
pH 7.5, 30°C
0.138
phosphatidic acid
pH 7.5, 30°C
additional information
additional information
-
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
inner segment of
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
the enzyme is mainly localized in close association with the membrane of the endoplasmic reticulum of the transfected cells. Several possible membrane-spanning regions
Manually annotated by BRENDA team
-
inner mitochondrial membrane
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
enzyme knockdown results in decreased mitochondrial CDP diacylglycerol synthase activity, decreased cardiolipin levels and a decrease in oxygen consumption
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CDS1_RAT
461
7
52970
Swiss-Prot
other Location (Reliability: 4)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 55000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
24 h, enzyme in extract from microsomal membranes loses 50% of its activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-72°C, the enzyme extract from microsomal membranes is indefinitely stable
-
4°C, the enzyme extract from microsomal membranes is stable for at least 24 h
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mok, A.Y.P.; McDougall, G.E.; McMurray, W.C.
CDP-diacylglycerol synthesis in rat liver mitochondria
FEBS Lett.
312
236-240
1992
Rattus norvegicus
Manually annotated by BRENDA team
Liteplo, R.G.; Sribney, M.
The stimulation of rat liver microsomal CTP: phosphatidate cytidylyltransferase activity by guanosine triphosphate
Biochim. Biophys. Acta
619
660-668
1980
Rattus norvegicus
Manually annotated by BRENDA team
Sribney, M.; Dove, J.L.; Lyman, E.M.
Studies on the synthesis of CDP-diacylglycerol: stimulation by GTP and inhibition by ATP and fluoride
Biochim. Biophys. Acta
79
749-755
1977
Rattus norvegicus
Manually annotated by BRENDA team
Bishop, H.H.; Strickland, K.P.
Studies on the formation by rat brain preparations of CDP-diglyceride from CTP and phosphatidic acids of varying fatty acid compositions
Can. J. Biochem.
54
249-260
1976
Rattus norvegicus
Manually annotated by BRENDA team
Sturton, R.G.; Brindley, D.N.
Factors controlling the activities of phosphatidate phosphohydrolase and phosphatidate cytidylyltransferase. The effects of chlorpromazine, demethylimipramine, cinchocaine, norfenfluramine, mepyramine and magnesium ions
Biochem. J.
162
25-32
1977
Rattus norvegicus
Manually annotated by BRENDA team
Van Heusden, G.P.H.; Van den Bosch, H.
The influence of exogenous and of membrane-bound phosphatidate concentration on the activity of CTP: phosphatidate cytidylyltransferase and phosphatidate phosphohydrolase
Eur. J. Biochem.
84
405-412
1978
Rattus norvegicus
Manually annotated by BRENDA team
Heacock, A.M.; Agranoff, B.W.
CDP-diacylglycerol synthase from mammalian tissues
Biochim. Biophys. Acta
1348
166-172
1997
Cavia porcellus, Drosophila sp. (in: flies), Escherichia coli, Homo sapiens, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Monaco, M.E.; Feldman, M.
Extraction and stabilization of mammalian CDP-diacylglycerol synthase activity
Biochem. Biophys. Res. Commun.
239
166-170
1997
Rattus norvegicus
Manually annotated by BRENDA team
Saito, S.; Goto, K.; Tonosaki, A.; Kondo, H.
Gene cloning and characterization of CDP-diacylglycerol synthase from rat brain
J. Biol. Chem.
272
9503-9509
1997
Rattus norvegicus (O35052)
Manually annotated by BRENDA team
Jiang, Y.J.; Lu, B.; Xu, F.Y.; Gartshore, J.; Taylor, W.A.; Halayko, A.J.; Gonzalez, F.J.; Takasaki, J.; Choy, P.C.; Hatch, G.M.
Stimulation of cardiac cardiolipin biosynthesis by PPARalpha activation
J. Lipid Res.
45
244-252
2004
Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Blunsom, N.J.; Gomez-Espinosa, E.; Ashlin, T.G.; Cockcroft, S.
Mitochondrial CDP-diacylglycerol synthase activity is due to the peripheral protein, TAMM41 and not due to the integral membrane protein, CDP-diacylglycerol synthase 1
Biochim. Biophys. Acta Mol. Cell Biol. Lipids
1863
284-298
2018
Rattus norvegicus (D3ZKT0)
Manually annotated by BRENDA team