Information on EC 2.7.7.41 - phosphatidate cytidylyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.7.7.41
-
RECOMMENDED NAME
GeneOntology No.
phosphatidate cytidylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
show the reaction diagram
-
-
-
-
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
show the reaction diagram
sequential bi-bi reaction: CDPdiacylglycerol synthase binds to CTP prior to phosphatidate and diphosphate is released prior to CDPdiacylglycerol in the reaction sequence
-
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
show the reaction diagram
ping-pong mechanism
-
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
show the reaction diagram
sequential bi bi reaction mechanism
-
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
show the reaction diagram
sequential mechanism
-
CTP + phosphatidate = diphosphate + CDP-diacylglycerol
show the reaction diagram
sequential ordered reaction mechanism
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
CDP-diacylglycerol biosynthesis I
-
CDP-diacylglycerol biosynthesis II
-
CDP-diacylglycerol biosynthesis III
-
Glycerophospholipid metabolism
-
Metabolic pathways
-
SYSTEMATIC NAME
IUBMB Comments
CTP:phosphatidate cytidylyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
CDP-DAG synthase
-
-
-
-
CDP-DG
-
-
-
-
CDP-DG synthetase
-
-
-
-
CDP-diacylglyceride synthetase
-
-
-
-
CDP-diacylglycerol synthase
-
-
-
-
CDP-diacylglycerol synthase
-
-
CDP-diacylglycerol synthase
-
-
CDP-diacylglycerol synthase
Synechocystis sp. PCC6803
-
-
-
CDP-diglyceride pyrophosphorylase
-
-
-
-
CDP-diglyceride synthetase
-
-
-
-
CDPdiglyceride pyrophosphorylase
-
-
-
-
CDS
-
-
-
-
CDS
-
-
CTP-diacylglycerol synthetase
-
-
-
-
CTP:1,2-diacylglycerophosphate-cytidyl transferase
-
-
-
-
CTP:phosphatidate cytidylyltransferase
-
-
-
-
cytidine 5'-diphosphate diacylglycerol synthase
-
-
cytidine 5'-diphosphate diacylglycerol synthase
Synechocystis sp. PCC6803
-
-
-
cytidine diphosphoglyceride pyrophosphorylase
-
-
-
-
cytidylyltransferase, phosphatidate
-
-
-
-
phosphatidate cytidyltransferase
-
-
-
-
phosphatidate cytidyltransferase 2
Q99L43
-
phosphatidic acid cytidylyltransferase
-
-
-
-
DAG synthetase
-
-
-
-
additional information
P98191, Q99L43
two different enzymes termed Cds1 and Cds2 with different genetical localization and different expression pattern
CAS REGISTRY NUMBER
COMMENTARY
9067-83-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain Marburg
-
-
Manually annotated by BRENDA team
Bacillus subtilis Marburg
strain Marburg
-
-
Manually annotated by BRENDA team
K12 strain DH1 with plasmid pCD100 causing 53fold overproduction of the enzyme
-
-
Manually annotated by BRENDA team
Micrococcus cerificans
-
-
-
Manually annotated by BRENDA team
Cds1; Cds1
SwissProt
Manually annotated by BRENDA team
Cds2; Cds2
SwissProt
Manually annotated by BRENDA team
male C57BL/6N x Sv/129 mice and peroxisome proliferator-activated receptor alpha (PPARalpha) null mice, mice fed ethyl-p-chlorophenoxyisobutyrate (clofibrate) for 14 days, activity is increased by 3-fold in normal mice fed clofibrate but not in PPARalpha null mice
-
-
Manually annotated by BRENDA team
cultured H9c2 cells
-
-
Manually annotated by BRENDA team
hog
-
-
Manually annotated by BRENDA team
strain PCC6803
-
-
Manually annotated by BRENDA team
Synechocystis sp. PCC6803
strain PCC6803
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-, Q8ILZ6
enzyme can substitute for the corresponding enzyme in Plasmodium knowlesi. Both the C-terminal cytidylyltransferase domain and the N-terminal extension are essential to Plasmodium spp.
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + 1,2-diarachidonoyl phosphatidic acid
diphosphate + CDP-1,2-diarachidonoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1,2-dicaproyl phosphatidic acid
diphosphate + CDP-1,2-dicaproylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1,2-dioleoyl phosphatidic acid
diphosphate + CDP-dioleoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1,2-dioleoyl phosphatidic acid
diphosphate + CDP-dioleoylglycerol
show the reaction diagram
-
reaction with 1-oleoyl-2-palmitoyl-phosphatidic acid at 87.5% of the activity with phosphatidic acid from egg phosphatidylcholine
-
-
-
CTP + 1,2-dipalmitoyl phosphatidic acid
diphosphate + CDP-dipalmitoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1,2-dipalmitoyl phosphatidic acid
diphosphate + CDP-dipalmitoylglycerol
show the reaction diagram
-
-
-
-
-
CTP + 1,2-dipalmitoyl phosphatidic acid
diphosphate + CDP-dipalmitoylglycerol
show the reaction diagram
-
reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 4.6% of the activity with phosphatidic acid from egg phosphatidylcholine
-
-
?
CTP + 1,2-distearoyl phosphatidic acid
diphosphate + CDP-1,2-distearoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-arachidonoyl-2-stearoyl phosphatidic acid
diphosphate + CDP-1-arachidonoyl-2-stearoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-oleoyl-2-palmitoyl phosphatidic acid
diphosphate + CDP-1-oleoyl-2-palmitoylglycerol
show the reaction diagram
-
reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 83.4% of the activity with phosphatidic acid from egg phosphatidylcholine
-
-
?
CTP + 1-oleoyl-2-stearoyl phosphatidic acid
diphosphate + CDP-1-oleoyl-2-stearoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-palmitoyl-2-oleoyl phosphatidic acid
diphosphate + CDP-1-palmitoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-palmitoyl-2-oleoyl phosphatidic acid
diphosphate + CDP-1-palmitoyl-2-oleoylglycerol
show the reaction diagram
-
reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 99.7% of the activity with phosphatidic acid from egg phosphatidylcholine
-
-
?
CTP + 1-stearoyl-2-arachidonoyl phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
show the reaction diagram
O35052
-
-
-
?
CTP + 1-stearoyl-2-oleoyl phosphatidic acid
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
r
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
Q92903
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
P38221
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
r
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
Q9NIH5
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
equilibrium constant is 0.001. Reverse reaction is favored in vitro
-
-
r
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
reaction with 1,2-dioleoylphosphatidic acid. Varying the fatty acid composition in the phosphatidic acids added exogenously gives the following order of decreasing activity: 1-stearoyl-2-oleoylphosphatidic acid, 1-oleoyl-2-stearoylphosphatidic acid, 1,2-dioleoylphosphatidic acid, 1-palmitoyl-2-oleoylphosphatidic acid, 1-stearoyl-2-arachidonoylphosphatidic acid, 1-arachidonoyl-2-stearoylphosphatidic acid, 1,2-diarachidonoylphosphatidic acid, 1,2-dicaproylphosphatidic acid, 1,2-dipalmitoylphosphatidic acid, 1,2-distearoylphosphatidic acid
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
didecanoyl phosphatidic acid is the most active of the synthetic phosphatidic acids tested, lysophosphatidic acid is a poor substrate
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme is specific for long-chain phosphatidic acid. 1-Acyl-sn-glycero-3-phosphate is not a substrate and phosphatidic acids with acyl chains shorter than 16 carbons are poor substrates
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
activity with phosphatidate obtained from egg lecithin which has a variety of mixed fatty acid in ester linkage. The phosphatidate has two oleoyl fatty acids is only 20% as effective as the phospatidate prepared from egg lecithin
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
O35052
the enzyme prefers 1-stearoyl-2-arachidonoyl phosphatidic acid as substrate. Little or no activity is detected towards phosphatidic acids containing saturated fatty acyl groups in both the sn-1 and sn-2 positions
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
high activity with phosphatidic acid from egg phosphatidylcholine
-
-
r
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
phosphatidic acid from egg lecithin and 1-stearoyl 2-arachidonoyl phosphatidic acid are preferred substrates for the microsomal enzyme
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
strong preference for phosphatidic acid with 16-carbon and 18-carbon length fatty acids and at least one unsaturated fatty acid
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
Micrococcus cerificans
-
highly specific for CTP
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
equilibrium constant is 0.22
-
-
r
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme shows a linear increase in activity with membrane-bound phosphatidate concentrations up to at least 100 nmol phosphatidate per mg of microsomal protein. The enzyme has a large reserve capacity and suggests that the enzyme is operating intracellularly, i.e. at phosphatidate concentrations of 5-10 mM/mg endoplasmic reticulum protein, far below the maximal capacity. The ratio of phosphatidate conversion into CDP diglyceride and 1,2-diglyceride seems to be constant for a large range of membrane-bounmd phosphatidate concentrations. The membrane-bound enzyme cannot utilize phosphatidate substrate present in heat denatured membranes, but is active on phosphatidate incorporated into membranes of phospholipid vesicles
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme is involved in the regulation of phospholipid metabolism
-
-
-
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
-
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
-
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
enzyme plays a central role in phospholipid biosynthesis
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
Q9NIH5
the enzyme catalyzes the synthesis of CDPdiacylglycerol, an obligatory intermediate compound in the biosynthesis of the major anionic and zwitterionic phospholipids
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme is required for the regeneration of the signalling molecule phosphatidylinositol-4,5-diphosphate from phosphatidic acid. A photoreceptor cell-specific isoform is a key regulator of phototransduction, a G-protein-coupled signalling cascade mediated by phospholipase C
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme plays a regulatory role in phototransduction by ensuring an adequate supply of phosphatidylinositol-4,5-diphosphate
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
regulation of phospholipid biosynthetic enzymes by the level of CDP-diacylglycerol synthase activity
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
Q92903
the level of CDP-diacylglycerol synthetase 1 is not a critical determinant of cellular phosphatidylinositol content. This argues against a determining role of the activity in the regulation of phosphatidylinositol biosynthesis
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
Micrococcus cerificans
-
enzyme is involved in synthesis of CDP-diglyceride, which plays a primary role in bacterial biosyntheses of essential phosphoglycerides
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the activity is essential for all phospholipid biosynthesis in Escherichia coli
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme produces key intermediates in phospholipid biosynthesis
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
P98191, Q99L43
-
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
-
involved in phospholipid synthesis
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
-
involved in the de novo biosynthesis of cardiolipin
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
P98191, Q99L43
involved in the synthesis of phospholipids and of phosphatidylinositol 4,5-diphosphate, which plays an important role in phosphoinositide-mediated signallng pathways
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
Synechocystis sp. PCC6803
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
Bacillus subtilis Marburg
-
-, involved in phospholipid synthesis
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
-
-
-
-
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
-
-
-
-
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
-
-
-
-
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
Micrococcus cerificans
-
50% of the activity with CTP
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
reaction at the same rate as CTP
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme is involved in the regulation of phospholipid metabolism
-
-
-
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
-
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
-
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
enzyme plays a central role in phospholipid biosynthesis
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
Q9NIH5
the enzyme catalyzes the synthesis of CDPdiacylglycerol, an obligatory intermediate compound in the biosynthesis of the major anionic and zwitterionic phospholipids
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme is required for the regeneration of the signalling molecule phosphatidylinositol-4,5-diphosphate from phosphatidic acid. A photoreceptor cell-specific isoform is a key regulator of phototransduction, a G-protein-coupled signalling cascade mediated by phospholipase C
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme plays a regulatory role in phototransduction by ensuring an adequate supply of phosphatidylinositol-4,5-diphosphate
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
regulation of phospholipid biosynthetic enzymes by the level of CDP-diacylglycerol synthase activity
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
Q92903
the level of CDP-diacylglycerol synthetase 1 is not a critical determinant of cellular phosphatidylinositol content. This argues against a determining role of the activity in the regulation of phosphatidylinositol biosynthesis
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
Micrococcus cerificans
-
enzyme is involved in synthesis of CDP-diglyceride, which plays a primary role in bacterial biosyntheses of essential phosphoglycerides
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the activity is essential for all phospholipid biosynthesis in Escherichia coli
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
show the reaction diagram
-
the enzyme produces key intermediates in phospholipid biosynthesis
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
-
involved in phospholipid synthesis
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
-
involved in the de novo biosynthesis of cardiolipin
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
P98191, Q99L43
involved in the synthesis of phospholipids and of phosphatidylinositol 4,5-diphosphate, which plays an important role in phosphoinositide-mediated signallng pathways
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
show the reaction diagram
Bacillus subtilis Marburg
-
involved in phospholipid synthesis
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
show the reaction diagram
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
activates at low concentrations, decrease in activity at higher concentrations
Co2+
-
activates at low concentrations, decrease in activity at higher concentrations
K+
Micrococcus cerificans
-
absolute requirement for
K+
-
stimulating effect increases with concentrations up to 75 mM
Mg2+
Micrococcus cerificans
-
stimulates, 3times more active than Mn2+, optimal activity at 10 mM
Mg2+
-
divalent cation required, Mn2+ or Mg2+, maximal activity at 5 mM Mg2+. Maximal activity obtained with Mg2+ is about 1.2fold greater than that obtained with Mn2+
Mg2+
-
maximal activity with 3-6 mM. 33% inhibition at 25 mM
Mg2+
-
maximal activation, about 15fold, at 10 mM
Mg2+
-
maximal activity at 20 mM Mg2+
Mg2+
-
maximal stimulation at 20 mM
Mg2+
-
optimal concentration is 60 mM
Mg2+
-
stimultes
Mg2+
-
50-60 mM required for optimal activity
Mg2+
-
-
Mg2+
-
required
Mg2+
-
the only divalent cation that supports activity
Mg2+
-
required
Mn2+
Micrococcus cerificans
-
stimulates
Mn2+
-
maximal activity at 18 mM
Mn2+
-
divalent cation required, Mn2+ or Mg2+, maximal activity at 1 mM Mn2+
Mn2+
-
1 mM, 70% of the maximal activity obtained with Mg2+
Mn2+
-
maximal activation, about 10fold, at 1 mM
Mn2+
-
up to 10 mM, never more than 15% as effective as Mg2+
NH4+
Micrococcus cerificans
-
only about half of the activity observed with K+
Rb+
Micrococcus cerificans
-
only about half of the activity observed with K+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
ATP
-
3 mM 50% inhibition. 5 mM, complete inhibition
ATP
-
slight inhibition below 0.175 mM
Ca2+
-
2 mM, 45% inhibition, even in presence of optimal Mg2+ concentrations
cardiolipin
-
the solubilized but not the microsomal enzyme is strongly inhibited
cardiolipin
-
2 mM, 27% inhibition
CDP-diacylglycerol
-
-
CDP-dipalmitin
-
inhibits diphosphorolysis of CDP-dipalmitin
CHAPS
-
at 0.3%
Co2+
-
2 mM, 58% inhibition, even in presence of optimal Mg2+ concentrations
CTP
-
competitive with dCTP
dCTP
-
competitive with respect to CTP
dCTP
-
2.5 mM, 54% inhibition of purified enzyme; competitive with respect to CTP
dCTP
-
competitive with respect to CTP
deoxycholate
-
partial inhibition
diphosphate
-
2.5 mM, 80% inhibition of purified enzyme, noncompetitive with respect to CTP and phosphatidic acid
F-
-
20 mM, completely abolishes the stimulatory effect of GTP
F-
-
20 mM NaF, 70% inhibition
FeSO4
-
2 mM, 97% inhibition, even in presence of optimal Mg2+ concentrations
Hg2+
-
0.1 mM, 90% inhibition
Hg2+
-
5 mM HgCl2, 78% inhibition
hydrazine
Micrococcus cerificans
-
-
lysolecithin
-
1 mM, 89% inhibition
lysophosphatidylcholine
-
the solubilized but not the microsomal enzyme is strongly inhibited
Mg2+
-
in presence of Mn2+
oleic acid
-
2 mM, 84% inhibition
palmitic acid
-
2 mM, 76% inhibition
palmitoyl-CoA
-
inhibits incorporation of CTP into CDP-diglyceride in presence or absence of added phosphatidic acid
palmitoyl-CoA
-
0.5 mM, 70% inhibition
PCMB
-
0.03 mM, 90% inhibition
PCMB
-
5 mM, 88% inhibition
phosphatidylethanolamine
-
2 mM, 16% inhibition
phosphatidylinositol
-
2 mM, 23% inhibition
phosphatidylinositol 4,5-bisphosphate
O35052
strong
phosphatidylserine
-
2 mM, 46% inhibition
Sodium deoxycholate
Micrococcus cerificans
-
-
Sodium deoxycholate
-
-
sodium lauryl sulfate
Micrococcus cerificans
-
-
Sulfram
Micrococcus cerificans
-
-
-
Thiophosphatidate
-
competitive
-
Triton X-100
-
above 20 mM
Triton X-100
-
-
Tween 20
Micrococcus cerificans
-
-
Tween 20
-
-
ZWT-12
-
2 mM, 86% inhibition
-
ZWT-14
-
2 mM, 92% inhibition
-
Mg2+
-
33% inhibition at 25 mM Mg2+
additional information
-
no inhibition by 1.65 mM ADP
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ATP
-
slight stimulation at high concentrations
ATP
-
stimulates only in presence of either lecithin or sphingomyelin
ATP
-
a maximal stimulation of about 3fold with CTP as substrate and 1.25fold with dCTP
Bovine serum albumin
-
activity is 6fold increased
-
cationic detergent
-
the phosphatidate must be emulsified in a cationic detergent for optimal activity
-
CHAPS
-
maximal activity at 0.5%
Cutsum
Micrococcus cerificans
-
enhances activity
-
GTP
-
stimulates
GTP
-
maximal stimulation at 1 mM; stimulates
GTP
-
stimulates only in presence of either lecithin or sphingomyelin
GTP
-
activity is 2fold enhanced
ITP
-
stimulates only in presence of either lecithin or sphingomyelin
lecithin
-
stimulates
non-ionic detergent
Micrococcus cerificans
-
absolute requirement
-
non-ionic detergent
-
absolute requirement
-
Norfenfluramine
-
can partially replace the divalent cation requirement
phosphatidylcholine
-
the solubilized but not the microsomal enzyme is activated
sphingomyelin
-
stimulates
Triton X-100
Micrococcus cerificans
-
stimulates
Triton X-100
-
5 mM, about 9fold stimulation
Triton X-100
-
maximal stimulation at 15 mM
UTP
-
stimulates only in presence of either lecithin or sphingomyelin
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.114
-
1,2-dioleoyl-sn-glycero-3-phosphate
O35052
pH 7.5, 30C
0.102
-
1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphate
O35052
pH 7.5, 30C
0.17
-
CTP
-
pH 7.2, 37C
0.18
-
CTP
-
pH 7.0, 30C
0.26
-
CTP
-
pH 7.5, 25C
0.6
-
CTP
-
pH 7.0, 37C
1
-
CTP
-
pH 6.5, 30C
0.26
-
dCTP
-
pH 7.5, 25C
0.58
-
dCTP
-
-
0.58
-
dCTP
-
pH 7.4, 30C
0.5
-
phosphatidate
-
-
0.5
-
phosphatidate
-
pH 6.5, 30C
0.65
-
phosphatidate
-
pH 7.0, 37C
2.5
-
phosphatidate
-
-
0.08
-
phosphatidic acid
-
pH 7.2, 37C
0.138
-
phosphatidic acid
O35052
pH 7.5, 30C
0.22
-
phosphatidic acid
-
pH 7.0, 30C
0.28
-
phosphatidic acid
-
-
0.28
-
phosphatidic acid
-
pH 7.4, 30C
0.28
-
phosphatidic acid
-
pH 7, 37C
0.3
-
phosphatidic acid
-
pH 7.5, 25C, with 0.45 mM CTP
0.9
-
dCTP
-
pH 7, 37C
additional information
-
additional information
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
8.92
-
CTP
-
pH 6.5, 30C
8.92
-
phosphatidate
-
pH 6.5, 30C
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.24
-
CDP
-
pH 7.2, 37C
5.6
-
CMP
-
pH 7.5, 25C
0.48
-
CTP
-
pH 7, 37C
0.59
-
CTP
-
pH 7.4, 30C
0.75
-
dCTP
-
pH 7, 37C
0.75
-
diphosphate
-
pH 7.5, 25C
1.5
-
diphosphate
-
pH 7.2, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00011
-
-
-
0.028
-
-
-
0.1333
-
Micrococcus cerificans
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.8
-
-
reaction with 1,2-dioleoylphosphatidic acid
7
-
-
Tris-HCl buffer
7.5
-
-
phosphate buffer
8
-
Micrococcus cerificans
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.8
7.6
-
pH 5.8: 60% of maximal activity, pH 7.6: about 55% of maximal activity
6
8.5
-
pH 6.0: 70% of maximal activity, pH 8.5: about 70% of maximal activity
6
9.3
Micrococcus cerificans
-
pH 6.0: about 45% of maximal activity, pH 9.3: about 40% of maximal activity
6.2
8.7
-
pH 6.2: 54% of maximal activity, pH 8.7: 56% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
45
-
Micrococcus cerificans
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
60
-
20C: about 35% of maximal activity, 60C: about 45% of maximal activity
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.6
-
P98191, Q99L43
calculated from the deduced amino acid sequence
7.57
-
-
calculated from nucleotide sequence
7.62
-
P98191, Q99L43
calculated from the deduced amino acid sequence
9.62
-
Q9NIH5
calculated from nucleotide sequence for full-length enzyme
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
embryonic
Manually annotated by BRENDA team
P98191, Q99L43
-
Manually annotated by BRENDA team
P98191, Q99L43
-
Manually annotated by BRENDA team
-
embryonic heart myoblastic cells, cells grown in cell culture and treated with ethyl-p-chlorophenoxyisobutyrate (clofibrate), no effect of clofibrate on enzyme activity
Manually annotated by BRENDA team
P98191, Q99L43
weak signal
Manually annotated by BRENDA team
O35052
inner segment of
Manually annotated by BRENDA team
P98191, Q99L43
expressed in photoreceptors but not in other layers of the retina; ganglion cell layer and inner nuclear layer, not expressed in photoreceptors
Manually annotated by BRENDA team
Q9NIH5
the enzyme is only weakly transcribed in ring stages, while major transcription occurs in the trophozoite and schizont stages
Manually annotated by BRENDA team
Q9NIH5
the enzyme is only weakly transcribed in ring stages, while major transcription occurs in the trophozoite and schizont stages
Manually annotated by BRENDA team
P98191, Q99L43
weak signal
Manually annotated by BRENDA team
P98191, Q99L43
-
Manually annotated by BRENDA team
-
high expression of the gene CDS1 in testis
Manually annotated by BRENDA team
P98191, Q99L43
weak signal
Manually annotated by BRENDA team
Q9NIH5
the enzyme is only weakly transcribed in ring stages, while major transcription occurs in the trophozoite and schizont stages
Manually annotated by BRENDA team
additional information
P98191, Q99L43
expressed in all tissues examined
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
associated with cell envelope fraction
Manually annotated by BRENDA team
P98191, Q99L43
associated to the membrane; associated to the membrane
Manually annotated by BRENDA team
-
cofractionation of enzyme and phosphatidylinositol synthase activities with cholesterol-poor, endoplasmic reticulum membranes. Enzymic activity localizes to endoplasmic reticulum membrane fragments containing calnexin and type III inositol (1,4,5)-trisphosphate receptors. CDP-diacylglycerol and phposphatidylinositol syntheses are restricted to a subfraction of the calnexin-positive endoplasmic reticulum
Manually annotated by BRENDA team
-
localized exclusively in the membrane fraction
Manually annotated by BRENDA team
-
integral protein of the inner membrane
Manually annotated by BRENDA team
-
the enzyme contains eight predicted transmembrane-spanning domains
Manually annotated by BRENDA team
O35052
the enzyme is mainly localized in close association with the membrane of the endoplasmic reticulum of the transfected cells. Several possible membrane-spanning regions
Manually annotated by BRENDA team
-
colocalizes with phospholipids in the domains located in the septal membrane
Manually annotated by BRENDA team
-, Q8ILZ6
peripheral membrane protein that is trafficked outside the parasite to the parasitophorous vacuole
Manually annotated by BRENDA team
Bacillus subtilis Marburg
-
colocalizes with phospholipids in the domains located in the septal membrane
-
Manually annotated by BRENDA team
-
about 20% of enzyme is associated with
-
Manually annotated by BRENDA team
-
mainly located in microsomal fraction
-
Manually annotated by BRENDA team
-
predominantly located in
-
Manually annotated by BRENDA team
-
inner mitochondrial membrane
Manually annotated by BRENDA team
-
main site of localization
Manually annotated by BRENDA team
-
inner mitochondrial membrane
Manually annotated by BRENDA team
-
5-10% of the cellular activity
Manually annotated by BRENDA team
-
enzyme is associated with both, the mitochondria and the endoplasmic reticulum
Manually annotated by BRENDA team
-, Q8ILZ6
peripheral membrane protein that is trafficked outside the parasite to the parasitophorous vacuole
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
114000
-
-
radiation inactivation
400000
-
-
approximately, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 27000, SDS-PAGE; x * 27570, calculation from gene sequence
?
-
x * 53226, calculation from nucleotide sequence
?
-
x * 48600, calculation from nucleotide sequence
?
-
x * 49100, calculation from nucleotide sequence
?
P98191, Q99L43
x * 51300, calculated from the deduced amino acid sequence; x * 52900, calculated from the deduced amino acid sequence
additional information
-
two polypeptides of 54000 Da and 56000 Da are detected by SDS-PAGE, it is possible that the 54000 Da subunit is a proteolysis product of the 56000 Da subunit
additional information
-
two distinct protein bands of 45000 Da and 19000 Da are detected by SDS-PAGE, it is yet unclear whether this represents simple aggregation of two unrelated proteins in a monomer dimer relationship, or whether the enzyme has two distinct subunits present in a 1:4 molar ratio
additional information
-
as determined from crystal structure the enzyme is a homodimer, each monomer comprises an alpha/beta fold with a central 3-2-1-4-5 parallel beta sheet
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
proteolytic modification
Q9NIH5
N-terminal proteolytical processing form a protein of 51000 Da and a protein of 28000 Da from the 78000 Da protein
proteolytic modification
-, Q8ILZ6
the 78 kDa predicted protein is recovered as a 50 kDa conserved C-terminal cytidylyltransferase domain C-CDS and a 28 kDa fragment that corresponds to the long hydrophilic asparagine-rich N-terminal extension N-CDS. The two fragments are the processed forms of the 78 kDa pro-form that is encoded from a single transcript with no alternate translation start site for C-CDS
additional information
P98191, Q99L43
different potential modification sites predicted from the deduced amino acid sequence; different potential modification sites predicted from the deduced amino acid sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging-drop method
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
stability is good at pH 7 and dropps rapidly above
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
-
24 h, enzyme in extract from microsomal membranes loses 50% of its activity
30
-
-
20 min, labile above
40
-
-
with detergent already inactivated at
55
-
Micrococcus cerificans
-
30 min, irreversible inactivation
57
-
-
30 min, no loss of activity of membrane-bound enzyme, 70% loss of activity of partially purified enzyme
60
-
-
30 min, membranous enzyme, stable up to
60
-
-
20 min, complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
stable to at least 2 cycles of freezing and thawing
-
solubilized enzyme is stable to freezing and thawing
-
the pure enzyme can be dialyzed, but to maintain activity the dialyzing buffer must contain 1% n-octyl-beta-D-glucopyranoside, 0.25 mM dithiothreitol and at least 200 mM salt
-
stable to at least 2 cycles of freezing and thawing
-
the solubilized protein is quite labile. Its stability is markedly enhanced by exchange dialysis against 10% glycerol, 20% propylene glycol, 25 mM cacodylate buffer, pH 6.5, 10 mM MgCl2
-
-20C, enzyme is very unstable when the disrupted lymphocyte homogenate or the isolated microsomes are stored, 75% loss of activity after 24 h. Stable for at least 1 month if stored in intact lymphocytes
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, cell envelope preparations stable for at least 2 months
-
-70C, solubilized enzyme is stable for at least several months
-
0-4C, solubilized enzyme is stable for at least several weeks
-
purified enzyme can be stored frozen for months if quick-frozen in liquid nitrogen
-
-72C, the enzyme extract from microsomal membranes is indefinitely stable
-
4C, the enzyme extract from microsomal membranes is stable for at least 24 h
-
-80C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months
-
-20C, 24 h, enzyme in isolated microsomes, 75% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
Micrococcus cerificans
-
mitochondrial enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed as GFP-fusion protein
-
expression in COS cells
Q92903
expression under the control of a GAL1 promoter in a null cds1 mutant yeast strain complements its growth defect and produces enzyme activity when induced with galactose
-
expressed in CHO-1 cells; expressed in CHO-1 cells
P98191, Q99L43
expression in Escherichia coli. Fusion protein of glutathione S-transferase and residues 108 to 424 of the Solanum tuberosum enzyme is produced by Escherichia coli cells transformed with the isopropyl-beta-galactopyranoside inducible plasmid pGEXstCD1
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the 78 kDa predicted protein is recovered as a 50 kDa conserved C-terminal cytidylyltransferase domain C-CDS and a 28 kDa fragment that corresponds to the long hydrophilic asparagine-rich N-terminal extension N-CDS. The two fragments are the processed forms of the 78 kDa pro-form that is encoded from a single transcript with no alternate translation start site for C-CDS
-, Q8ILZ6
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C102Y
P38221
mutation is the molecular basis for the inositol excretion phenotype
additional information
-
the cdsA-inactivated PAL mutant strain lacks phycobilisomes
additional information
Synechocystis sp. PCC6803
-
the cdsA-inactivated PAL mutant strain lacks phycobilisomes
-