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Information on EC 2.7.7.31 - DNA nucleotidylexotransferase and Organism(s) Bos taurus and UniProt Accession P06526

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IUBMB Comments
Catalyses template-independent extension of the 3'- end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. Nucleoside may be ribo- or 2'-deoxyribo-.
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Bos taurus
UNIPROT: P06526
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The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Synonyms
terminal deoxynucleotidyl transferase, terminal deoxynucleotidyl transferase (tdt), deoxynucleotidyl transferase, terminal deoxynucleotidyltransferase, terminal deoxynucleotide transferase, terminal deoxyribonucleotidyl transferase, rep75, terminal deoxyribonucleotidyltransferase, dna nucleotidylexotransferase, deoxynucleotidyl terminal transferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
terminal deoxynucleotidyl transferase
-
terminal deoxynucleotiyl transferase
-
addase
-
-
-
-
bTdT
-
-
deoxynucleotidyl terminal transferase
-
-
-
-
deoxynucleotidyl transferase
-
-
deoxyribonucleic acid nucleotidyltransferase
-
-
-
-
deoxyribonucleic nucleotidyltransferase
-
-
-
-
DNA-polymerizing enzyme
-
-
nucleotidyltransferase, terminal deoxyribo-
-
-
-
-
terminal addition enzyme
-
-
-
-
terminal deoxynucleotide transferase
-
-
-
-
terminal deoxynucleotidyl transferase
-
-
terminal deoxynucleotidyl transferase (TDT)
-
-
terminal deoxynucleotidyltransferase
terminal deoxyribonucleotidyltransferase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
nucleoside-triphosphate:DNA deoxynucleotidylexotransferase
Catalyses template-independent extension of the 3'- end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. Nucleoside may be ribo- or 2'-deoxyribo-.
CAS REGISTRY NUMBER
COMMENTARY hide
9027-67-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-pyridone-2'-deoxyriboside triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
show the reaction diagram
-
-
-
?
4-bromo-2-pyridone-2'-deoxyriboside triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
show the reaction diagram
-
-
-
?
4-chloro-2-pyridone-2'-deoxyriboside triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
show the reaction diagram
-
-
-
?
4-hydroxy-2-pyridone-2'-deoxyriboside triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
show the reaction diagram
-
-
-
?
4-thio-2'-deoxyuridine triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
show the reaction diagram
-
-
-
?
5-carboxy-2'-deoxyuridine triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
show the reaction diagram
-
-
-
?
5-cyano-2'-deoxyuridine triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
show the reaction diagram
-
-
-
?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
?
Cy5-dCTP + 2bA FAM oligo
diphosphate + ?
show the reaction diagram
2bA FAM oligo is a primer with FAM at 2 bases from the 3' end (5'-CGCTTGCACAGGTGCGTT/iFluorT/CA-3')
-
-
?
dCTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
?
d[5-(3-aminophenyl)]CTP + DNA
diphosphate + ?
show the reaction diagram
5-substituted dCTPs give products elongated by 1 to 5 nucleotides, with most intense bands corresponding to 2-3 extra nucleotides
-
-
?
d[5-(3-aminophenyl)]UTP + DNA
diphosphate + ?
show the reaction diagram
5-substituted dUTPs give products elongated by 4 to 5 nucleotides, the maximum length of the tail is about 14 nucleotides
-
-
?
d[5-(3-nitrophenyl)]CTP + DNA
diphosphate + ?
show the reaction diagram
5-substituted dCTPs give products elongated by 1 to 5 nucleotides, with most intense bands corresponding to 2-3 extra nucleotides
-
-
?
d[5-(3-nitrophenyl)]UTP + DNA
diphosphate + ?
show the reaction diagram
5-substituted dUTPs give products elongated by 4 to 5 nucleotides, the maximum length of the tail is about 14 nucleotides
-
-
?
d[7-deaza-7-(3-aminophenyl)]ATP + DNA
diphosphate + ?
show the reaction diagram
the most intense band observed for reaction with 7-deaza,7-substituted dATPs corresponds to one nucleotide attached, but the products are rather polydisperse in lengths, reaching several tens of extra nucleotides
-
-
?
d[7-deaza-7-(3-aminophenyl)]GTP + DNA
diphosphate + ?
show the reaction diagram
-
-
-
?
d[7-deaza-7-(3-nitrophenyl)]ATP + DNA
diphosphate + ?
show the reaction diagram
the most intense band observed for reaction with 7-deaza,7-substituted dATPs corresponds to one nucleotide attached, but the products are rather polydisperse in lengths, reaching several tens of extra nucleotides
-
-
?
d[7-deaza-7-(3-nitrophenyl)]GTP + DNA
diphosphate + ?
show the reaction diagram
most useful substrate, efficiently incorporated by the transferase to form long tail-labels at any oligonucleotide
-
-
?
N4-amino-2'-deoxycytidine triphosphate + 5'-TAATACGACTCACTATAGGGAGA-3'
diphosphate + ?
show the reaction diagram
-
-
-
?
D-beta-deoxythymidine-triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
dATP + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
dATP + poly(dA)50
diphosphate + poly(dA)51
show the reaction diagram
-
-
-
-
?
dCTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
show the reaction diagram
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
dGTP + DNA
diphosphate + DNA
show the reaction diagram
-
-
-
-
?
dGTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
dTTP + DNAn
?
show the reaction diagram
-
-
-
-
?
ribonucleoside triphosphate + DNAn
diphosphate + ?
show the reaction diagram
-
-
-
-
?
ssDNA + ferrocene-labeled dideoxy-UTP
ssDNA-ddUFc + diphosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
?
deoxynucleoside triphosphate + DNA
diphosphate + DNAn+1
show the reaction diagram
ssDNA + ferrocene-labeled dideoxy-UTP
ssDNA-ddUFc + diphosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-triphosphate
-
-
1,10-phenanthroline
-
not m-isomer
2',3'-dideoxyadenosine
-
inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate ATP, 50% inhibition at 0.006 mM
2',3'-dideoxycytidine
-
inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate CTP, 50% inhibition at 0.002 mM
2',3'-dideoxyguanosine
-
inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate GTP, 50% inhibition at 0.005 mM
2',3'-dideoxythymidine
-
inhibition is stronger with (dA)12-18 as primer than with heat denatured DNA, substrate TTP, 50% inhibition at 0.020 mM
2',3'-dideoxythymidine 5'-triphosphate
-
-
2,2'-dipyridyl
-
-
3'-dATP
-
selective inhibitor, inactive in DNA-synthesizing systems both with DNA polymerase alpha or beta
3'-Deoxy-3'-fluorothymidine 5'-triphosphate
-
-
5-(hydroxymethyl)-2-furfural
-
HMF, a pyrolysate of carbohydrate isolated from instant coffee (CoVea arabica L.)
6-(1-phenyl-1H-indol-3-yl)-2,4-dioxy-5-hexenoic acid
-
IC50: 0.0472 mM
6-(1-phenylmethyl-1H-indol-3-yl)-2,4-dioxy-5-hexenoic acid
-
IC50: 0.0079 mM
6-(4-benzoyl-1-phenylmethyl-1H-pyrrol-2-yl)-2,4-dioxo-5-hexenoic acid
-
IC50: 0.0162 mM
9-alpha-Arabinofuranosyladenine 5'-triphosphate
-
-
9-beta-arabinofuranosyladenine 5'-triphosphate
-
-
ATP
-
selective inhibitor, inactive in DNA-synthesizing systems both with DNA polymerase alpha or beta
cysteine
-
-
dATP
-
mechanism of Mn2+-dependent inhibition
diadenosine 5',5'-diphosphate
-
-
diadenosine 5',5'-hexaphosphate
-
-
diadenosine 5',5'-pentaphosphate
-
-
diadenosine 5',5'-tetraphosphate
diadenosine 5',5'-triphosphate
-
-
diallyl trisulfide
-
-
diphosphate
-
-
EDTA
-
-
high ionic strength
-
-
-
histidine
-
-
L-beta-deoxythymidine-triphosphate
-
i.e. L-dTTP, competitive
L-dTTP
-
-
Mercaptoacetate
-
-
metal chelators
-
-
-
triethylenetetramine
-
-
Tris
-
above 50 mM
additional information
-
Allium sativa extract is a mixture of diallyl trisulfide, diallyl tetrasulfide, and diallyl pentasulfide exhibiting an IC50 value of 0.371 mM at 37°C and a KI value of 0.0121 mM at 37°C
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
stimulates
sulfhydryl compound
-
required
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00646 - 0.0176
2bA FAM oligo
-
0.1
D-beta-deoxythymidine-triphosphate
-
pH 6.6, 37°C, primer oligo(dT)20
0.004
dATP
-
37°C, primer (dA)12-18
0.071
dCTP
-
37°C, primer (dA)12-18
0.083 - 0.114
dGTP
0.248 - 1.02
dTTP
0.0003 - 0.0025
poly(dA)50
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0925 - 1.2
2bA FAM oligo
-
0.833
dATP
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.25 - 146
2bA FAM oligo
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-triphosphate
-
-
0.06
L-dTTP
-
pH 6.6, 37°C, primer oligo(dT)20
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0055
5-(hydroxymethyl)-2-furfural
Bos taurus
-
-
0.0472
6-(1-phenyl-1H-indol-3-yl)-2,4-dioxy-5-hexenoic acid
Bos taurus
-
IC50: 0.0472 mM
0.0079
6-(1-phenylmethyl-1H-indol-3-yl)-2,4-dioxy-5-hexenoic acid
Bos taurus
-
IC50: 0.0079 mM
0.0162
6-(4-benzoyl-1-phenylmethyl-1H-pyrrol-2-yl)-2,4-dioxo-5-hexenoic acid
Bos taurus
-
IC50: 0.0162 mM
0.144
diallyl trisulfide
Bos taurus
-
at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
precursor
Manually annotated by BRENDA team
-
precursor
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
enzyme is ubiquitylated by a BPOZ-2/Cul3 complex, as the ubiquitin ligase, and then degraded by the 26 S proteasome. Enzyme is ubiquitylated by the Cul3-based ubiquitylation system in vitro and may also be directly ubiquitylated by the E2 proteins UbcH5a/b/c and UbcH6, E3-independently. Ubiquitylation inhibits the nucleotidyltransferase activity
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TDT_BOVIN
509
0
58296
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26500
-
1 * 8000 (alpha) + 1 * 26500 (beta), SDS-PAGE
32360
-
equilibrium sedimentation
42000 - 60000
-
gel filtration, the 2 subunits of the calf thymus enzyme reported earlier may be proteolytic products derived from a single polypeptide of MW 60000, which may be the native form
44000
-
2 high MW forms: 58000 and 45000 and one two subunit form of 44000 MW
45000
-
2 high MW forms: 58000 and 45000 and one two subunit form of 44000 MW
58000
-
2 high MW forms: 58000 and 45000 and one two subunit form of 44000 MW
60000
-
-
79000
-
-
8000
-
1 * 8000 (alpha) + 1 * 26500 (beta), SDS-PAGE
additional information
-
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 45000-47000, SDS-PAGE
dimer
-
1 * 8000 (alpha) + 1 * 26500 (beta), SDS-PAGE
monomer
-
1 * 79000
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K177N/E178G/K271E/F280L/M283K/H405P
the thermostable mutant TdT3-2 shows a 10 C increased melting temperature as compared to the wild type enzyme. The mutant is capable of incorporating higher amount of ddCTP onto double stranded blunt end DNA
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
stable at
643200, 643201
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41 - 47
the melting temperature of the wild type enzyme is 41.5-45.7 C. At 47 C, the wild type enzyme is denatured within 5 min. At 58 C,the wild type enzyme is not active
40
-
not stable above
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
not stable in urea, SDS and organic solvents
-
rapid loss of activity can be eliminated by addition of albumin to the reaction mixture
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 40% loss of activity after 7 months
-
-20°C, 50 mM potassium phosphate buffer, pH 7.0, stable for 9 years
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-Sepharose column chromatography and Q Sepharose column chromatography
2 high MW forms: 58000 and 45000 and one two subunit form of 44000 MW
-
single step immunoaffinity purification
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
-
expression in Escherichia coli and in 293T cell
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
DNA tail-labelling using terminal deoxynucleotidyl transferase and modified deoxynucleoside triphosphates. Amino- and nitrophenyl-modified dNTPs are good substrates giving 3'-end stretches of different lengths depending on the nucleotide and concentration. d[7-Deaza-7-(3-nitrophenyl)]GTP is efficiently incorporated by the transferase to form long tail-labels at any oligonucleotide, resulting in a considerable enhancement of voltammetric signals due to the nitro group reduction. Discrimination between complementary and non-complementary target DNAs sequences by tail-labelled hybridization probes is easily accomplished, and tumour suppressor p53 protein is able to recognize a specific binding site within tail-labelled DNA substrates
analysis
-
reliable and accurate 3'-end miRNA-labeling method for microarray detection by terminal-deoxynucleotidyl transferase TdT. Using its ability to add polynucleotides at a RNA receptor molecule by using deoxycytidine triphosphate, miRNA is successfully labeled by adding fluorescence deoxycytidine triphosphates to its 3'-end. The TdT-labeling method can detect as little as 0.04 fmol of synthetic small RNA, and produce precise and accurate measurements that span a linear dynamic range from 0.04 to 5 fmol of synthetic small RNA
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Krakow, J.S.; Coutsogeorgopoulos, C.; Canellakis, E.S.
Studies on the incorporation of deoxyribonucleotides and ribonucleotides into deoxyribonucleic acid
Biochim. Biophys. Acta
55
639-650
1962
Bos taurus
Manually annotated by BRENDA team
Gottesman, M.E.; Canellakis, E.S.
The terminal nucleotidyltransferases of calf thymus nuclei
J. Biol. Chem.
241
4339-4352
1966
Bos taurus
Manually annotated by BRENDA team
Chang, L.M.S.; Bollum, F.J.
Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase
J. Biol. Chem.
246
909-916
1971
Bos taurus
Manually annotated by BRENDA team
Pandey, V.; Modak, M.J.
Purification of high molecular mass species of calf thymus terminal deoxynucleotidyltransferase
Prep. Biochem.
17
359-377
1987
Bos taurus
Manually annotated by BRENDA team
Matthes, E.; Lehman, C.; Drescher, B.; Buettner, W.; Langen, P.
3-Deoxy-3-fluorothymidinetriphosphate: inhibitor and terminator of DNA synthesis catalysed by DNA polymerase beta, terminal deoxynucleotidyl transferase and DNA polymerase I
Biomed. Biochim. Acta
44
K63-K73
1985
Bos taurus
Manually annotated by BRENDA team
Deibel, M.R.; Coleman, M.S.; Acree, K.; Hutton, J.J.
Biochemical and immunological properties of human terminal deoxynucleotidyl transferase purified from blasts of acute lymphoblastic and chronic myelogenous leukemia
J. Clin. Invest.
67
725-734
1981
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Ratliff, R.L.
Terminal deoxyribonucleotidyl transferase
The Enzymes,3rd Ed. (Boyer,P. D. ,ed. )
14
105-118
1981
Bos taurus, Homo sapiens, Mus musculus
-
Manually annotated by BRENDA team
Bollum, F.J.
Terminal deoxyribonucleotidyl transferase
The Enzymes,3rd Ed. (Boyer,P. D. ,ed. )
10
145-171
1974
Bos taurus
-
Manually annotated by BRENDA team
Bollum, F.J.
Terminal deoxynucleotidyl transferase: biological studies
Adv. Enzymol. Relat. Areas Mol. Biol.
47
347-374
1978
Bos taurus
Manually annotated by BRENDA team
Mller, W.E.G.; Zahn, R.K.; Arendes, J.
Differential mode of inhibition of terminal deoxynucleotidyl transferase by 3-dATP, ATP, betaaraATP and alphaaraATP
FEBS Lett.
94
47-50
1978
Bos taurus
Manually annotated by BRENDA team
Coleman, M.S.
Terminal deoxynucleotidyl transferase: characterization of extraction and assay conditions from human and calf tissue
Arch. Biochem. Biophys.
182
525-532
1977
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Ono, K.; Nakane, H.; Colla, L.; De Clercq, E.
Inhibition of terminal deoxyribonucleotidyltransferase by (E)-5-(2-bromovinyl)-2-deoxyuridine 5-triphosphate
Nucleic Acids Res.
12
123-126
1983
Bos taurus
-
Manually annotated by BRENDA team
Ono, K.; Iwata, Y.; Nakamura, H.; Matsukage, A.
Selective inhibition of terminal deoxynucleotidyl transferase by diadenosine 5,5-P1,P4-tetraphosphate
Biochem. Biophys. Res. Commun.
95
34-40
1980
Bos taurus
Manually annotated by BRENDA team
Fuller, S.A.; Philips, A.; Coleman, M.S.
Affinity purification and refined structural characterization of terminal deoxynucleotidyltransferase
Biochem. J.
231
105-113
1985
Bos taurus
Manually annotated by BRENDA team
Ono, K.
Inhibitory effects of various 2,3-dideoxynucleoside 5-triphosphates on the utilization of 2-deoxynucleoside 5-triphosphates by terminal deoxynucleotidyltransferase from calf thymus
Biochim. Biophys. Acta
1049
15-20
1990
Bos taurus
Manually annotated by BRENDA team
Pandey, V.N.; Amrute, S.B.; Satav, J.G.; Modak, M.J.
Inhibition of terminal deoxynucleotidyl trasnferase by adenine dinucleotides
FEBS Lett.
213
205-208
1987
Bos taurus
-
Manually annotated by BRENDA team
Srivastava, A.; Modak, M.J.
Biochemistry of terminal deoxynucleotidyltransferase: identification, characterization, requirements, and active-site involvement in the catalysis of associated pyrophosphate exchange and pyrophosphorolytic activity
Biochemistry
19
3270-3275
1980
Bos taurus
Manually annotated by BRENDA team
Modak, M.J.
Biochemistry of terminal deoxynucleotidyltransferase. Mechanism of manganese-dependent inhibition by deoxyadenosine 5-triphosphate and biological implications
Biochemistry
18
2679-2684
1979
Bos taurus
Manually annotated by BRENDA team
Nakamura, H.; Tanabe, K.; Yoshida, S.; Morita, T.
Terminal deoxynucleotidyltransferase of 60,000 daltons from mouse, rat, and calf thymus. Purification by immunoadsorbent chromatography and comparison of peptide structures
J. Biol. Chem.
256
8745-8751
1981
Bos taurus, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Okamura, S.; Crane, F.; Messner, H.A.; Mak, T.W.
Purification of terminal deoxynucleotidyltransferase by oligonucleotide affinity chromatography
J. Biol. Chem.
253
3765-3767
1978
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Yamadori, I.; Yoshino, T.; Kondo, E.; Cao, L.; Akagi, T.; Matsuo, Y.; Minowada, J.
Comparison of two methods of staining apoptotic cells of leukemia cell lines: terminal deoxynucleotidyl transferase and DNA polymerase I reactions
J. Histochem. Cytochem.
46
85-90
1998
Bos taurus
Manually annotated by BRENDA team
Focher, F.; Maga, G.; Bendiscioli, A.; Capobianco, M.; Colonna, F.; Garbesi, A.; Spadari, S.
Stereospecificity of human DNA polymerases alpha, beta, gamma, delta and epsilon, HIV-reverse transcriptase, HSV-1 DNA polymerase, calf thymus terminal transferase and Escherichia coli DNA polymerase I in recognizing D- and L-thymidine 5'-triphosphate as substrate
Nucleic Acids Res.
23
2840-2847
1995
Bos taurus
Manually annotated by BRENDA team
Koziolkiewicz, M.; Maciaszek, A.; Stec, W.J.; Semizarov, D.; Victorova, L.; Krayevsky, A.
Effect of P-chirality of oligo(deoxyribonucleoside phosphorothioate)s on the activity of terminal deoxyribonucleotidyl transferase
FEBS Lett.
434
77-82
1998
Bos taurus
Manually annotated by BRENDA team
Arzumanov, A.A.; Victorova, L.S.; Jasko, M.V.; Yesipov, D.S.; Krayevsky, A.A.
Terminal deoxynucleotidyl transferase catalyzes the reaction of DNA phosphorylation
Nucleic Acids Res.
28
1276-1281
2000
Bos taurus
Manually annotated by BRENDA team
Thai, T.H.; Kearney, J.F.
Isoforms of terminal deoxynucleotidyltransferase: Developmental aspects and function
Adv. Immunol.
86
113-136
2005
Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Locatelli, G.A.; Di Santo, R.; Crespan, E.; Costi, R.; Roux, A.; Hubscher, U.; Shevelev, I.; Blanca, G.; Villani, G.; Spadari, S.; Maga, G.
Diketo hexenoic acid derivatives are novel selective non-nucleoside inhibitors of mammalian terminal deoxynucleotidyl transferases, with potent cytotoxic effect against leukemic cells
Mol. Pharmacol.
68
538-550
2005
Bos taurus
Manually annotated by BRENDA team
Mizushina, Y.; Yagita, E.; Kuramochi, K.; Kuriyama, I.; Shimazaki, N.; Koiwai, O.; Uchiyama, Y.; Yomezawa, Y.; Sugawara, F.; Kobayashi, S.; Sakaguchi, K.; Yoshida, H.
5-(Hydroxymethyl)-2-furfural: a selective inhibitor of DNA polymerase lambda and terminal deoxynucleotidyltransferase
Arch. Biochem. Biophys.
446
69-76
2006
Bos taurus
Manually annotated by BRENDA team
Fowler, J.D.; Suo, Z.
Biochemical, structural, and physiological characterization of terminal deoxynucleotidyl transferase
Chem. Rev.
106
2092-2110
2006
Bos taurus, Mus musculus
Manually annotated by BRENDA team
Cho, Y.; Kool, E.T.
Enzymatic synthesis of fluorescent oligomers assembled on a DNA backbone
ChemBioChem
7
669-672
2006
Bos taurus
Manually annotated by BRENDA team
Anne, A.; Bonnaudat, C.; Demaille, C.; Wang, K.
Enzymatic redox 3-end-labeling of DNA oligonucleotide monolayers on gold surfaces using terminal deoxynucleotidyl transferase (TdT)-mediated single base extension
J. Am. Chem. Soc.
129
2734-2735
2007
Bos taurus
Manually annotated by BRENDA team
Nishida, M.; Hada, T.; Kuramochi, K.; Yoshida, H.; Yonezawa, Y.; Kuriyama, I.; Sugawara, F.; Yoshida, H.; Mizushina, Y.
Diallyl sulfides: Selective inhibitors of family X DNA polymerases from garlic (Allium sativum L.)
Food Chem.
108
551-560
2008
Bos taurus
Manually annotated by BRENDA team
Zhao, B.; Gong, Z.; Ma, Z.; Wang, D.; Jin, Y.
Simple and sensitive microRNA labeling by terminal deoxynucleotidyl transferase
Acta Biochim. Biophys. Sin. (Shanghai)
44
129-135
2012
Bos taurus
Manually annotated by BRENDA team
Horakova, P.; Macickova-Cahova, H.; Pivonkova, H.; Spacek, J.; Havran, L.; Hocek, M.; Fojta, M.
Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl transferase. Application in electrochemical DNA hybridization and protein-DNA binding assays
Org. Biomol. Chem.
9
1366-1371
2011
Bos taurus (P06526)
Manually annotated by BRENDA team
Maezawa, S.; Fukushima, R.; Matsushita, T.; Kato, T.; Takagaki, Y.; Nishiyama, Y.; Ando, S.; Matsumoto, T.; Kouda, K.; Hayano, T.; Suzuki, M.; Koiwai, K.; Koiwai, O.
Ubiquitylation of terminal deoxynucleotidyltransferase inhibits its activity
PLoS ONE
7
e39511
2012
Bos taurus
Manually annotated by BRENDA team
Chua, J.P.S.; Go, M.K.; Osothprarop, T.; Mcdonald, S.; Karabadzhak, A.G.; Yew, W.S.; Peisajovich, S.; Nirantar, S.
Evolving a thermostable terminal deoxynucleotidyl transferase
ACS Synth. Biol.
9
1725-1735
2020
Bos taurus (P06526)
Manually annotated by BRENDA team
Tauraite, D.; Jakubovska, J.; Dabuzinskaite, J.; Bratchikov, M.; Meskys, R.
Modified nucleotides as substrates of terminal deoxynucleotidyl transferase
Molecules
22
672
2017
Bos taurus (P06526)
Manually annotated by BRENDA team
Wang, Y.; Liu, J.; Zhou, H.
Visual detection of cucumber green mottle mosaic virus based on terminal deoxynucleotidyl transferase coupled with DNAzymes amplification
Sensors
19
1298
2019
Bos taurus (P06526)
Manually annotated by BRENDA team