Information on EC 2.7.7.28 - nucleoside-triphosphate-aldose-1-phosphate nucleotidyltransferase

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The expected taxonomic range for this enzyme is: Eutheria

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
2.7.7.28
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
nucleoside-triphosphate-aldose-1-phosphate nucleotidyltransferase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nucleoside triphosphate + alpha-D-aldose 1-phosphate = diphosphate + NDP-hexose
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nucleotidyl group transfer
-
-
-
-
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
NTP:alpha-D-aldose-1-phosphate nucleotidyltransferase
In decreasing order of activity, guanosine, inosine and adenosine diphosphate hexoses are substrates in the reverse reaction, with either glucose or mannose as the sugar.
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
EC 2.7.7.29
-
-
formerly
-
GDP hexose pyrophosphorylase
-
-
-
-
GDPhexose pyrophosphorylase
-
-
-
-
GTP:alpha-D-hexose-1-phosphate guanylyltransferase
-
-
-
-
guanosine diphosphohexose pyrophosphorylase
-
-
-
-
guanylyltransferase, hexose 1-phosphate
-
-
-
-
hexose 1-phosphate guanylyltransferase
-
-
-
-
hexose 1-phosphate nucleotidyltransferase
-
-
-
-
hexose nucleotidylating enzyme
-
-
-
-
hexose-1-phosphate guanylyltransferase
-
-
-
-
NDP hexose pyrophosphorylase
-
-
-
-
NTP:hexose-1-phosphate nucleotidyltransferase
-
-
-
-
nucleoside diphosphohexose pyrophosphorylase
-
-
-
-
nucleoside-triphosphate-hexose-1-phosphate nucleotidyltransferase
-
-
-
-
nucleotidyltransferase, hexose 1-phosphate
-
-
-
-
SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
37278-26-5
-
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SplaateGeneral_Information, Organism, Commentary, Literature
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADPmannose + diphosphate
ATP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
GDP-glucose + diphosphate
GTP + D-glucose 1-phosphate
show the reaction diagram
-
best substrate
-
r
GDP-glucose + diphosphate
GTP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
r
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
reaction at about half the rate of GDPglucose
-
-
IDP-glucose + diphosphate
ITP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
IDP-glucose + diphosphate
ITP + D-glucose 1-phosphate
show the reaction diagram
-
reaction at 21% the rate of GDPglucose
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
reaction at 7% the rate of GDPglucose
-
-
UDPglucose + diphosphate
UTP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
UDPmannose + diphosphate
UTP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADPmannose + diphosphate
ATP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
GDP-glucose + diphosphate
GTP + D-glucose 1-phosphate
show the reaction diagram
-
best substrate
-
r
GDP-glucose + diphosphate
GTP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
r
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
reaction at about half the rate of GDPglucose
-
-
IDP-glucose + diphosphate
ITP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
IDP-glucose + diphosphate
ITP + D-glucose 1-phosphate
show the reaction diagram
-
reaction at 21% the rate of GDPglucose
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
reaction at 7% the rate of GDPglucose
-
-
UDPglucose + diphosphate
UTP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
UDPmannose + diphosphate
UTP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
SplaateCofactor,Organism,Commentary,Literature,Filename
SplaateMetals_Ions,Organism,Commentary, Literature
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
-
metal requirement can be met by Co2+
Mg2+
-
requirement, maximal activity at about 5 mM, maximal activity at about twice the diphosphate-concentration
Mg2+
-
required
Mn2+
-
metal requirement can be partially met by Mn2+
SplaateInhibitors, Organism, Commentary, Literature,Filename
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
D-mannose 1-phosphate
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strong
D-mannose 1-phosphate
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-
GDPmannose
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strong
GDPmannose
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-
Zn2+
-
inhibits when added with Mg2+, Mn2+ or Co2+
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.6
D-glucose 1-phosphate
-
pH 7.8, 25C
2.6
D-glucose 1-phosphate
-
-
0.082
diphosphate
-
pH 7.8, 25C
0.082
diphosphate
-
-
0.62
GDPglucose
-
pH 7.8, 25C
0.0001
GDPmannose
-
pH 7.8, 25C
0.0001
GDPmannose
-
-
2.6
GTP
-
pH 7.8, 25C
2.6
GTP
-
-
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.047
D-mannose 1-phosphate
-
pH 7.8, 25C
0.047
D-mannose 1-phosphate
-
-
0.00017
GDP-mannose
-
pH 7.8, 25C
0.00017
GDP-mannose
-
-
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5 - 8
-
-
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 9
-
pH 6.5: about 40% of maximal activity, pH 9.0: about half-maximal activity
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
SplaateSubunits, Organism, Commentary, Literature
SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 6.5
-
t1/2: 60 days at 4C
645219
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
-
15% loss of activity after 5 min, 33% loss of activity after 10 min
645219
55
-
1 min, 57% loss of activity
645219
SplaateGeneral_Stability, Organism, Literature
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, at pH 6-6.5, t1/2: 60 days
-
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SplaateCommentary, Organism, Literature
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature