Information on EC 2.7.7.28 - nucleoside-triphosphate-aldose-1-phosphate nucleotidyltransferase

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
2.7.7.28
-
RECOMMENDED NAME
GeneOntology No.
nucleoside-triphosphate-aldose-1-phosphate nucleotidyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
nucleoside triphosphate + alpha-D-aldose 1-phosphate = diphosphate + NDP-hexose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
NTP:alpha-D-aldose-1-phosphate nucleotidyltransferase
In decreasing order of activity, guanosine, inosine and adenosine diphosphate hexoses are substrates in the reverse reaction, with either glucose or mannose as the sugar.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
EC 2.7.7.29
-
-
formerly
-
GDP hexose pyrophosphorylase
-
-
-
-
GDPhexose pyrophosphorylase
-
-
-
-
GTP:alpha-D-hexose-1-phosphate guanylyltransferase
-
-
-
-
guanosine diphosphohexose pyrophosphorylase
-
-
-
-
guanylyltransferase, hexose 1-phosphate
-
-
-
-
hexose 1-phosphate guanylyltransferase
-
-
-
-
hexose 1-phosphate nucleotidyltransferase
-
-
-
-
hexose nucleotidylating enzyme
-
-
-
-
hexose-1-phosphate guanylyltransferase
-
-
-
-
NDP hexose pyrophosphorylase
-
-
-
-
NTP:hexose-1-phosphate nucleotidyltransferase
-
-
-
-
nucleoside diphosphohexose pyrophosphorylase
-
-
-
-
nucleoside-triphosphate-hexose-1-phosphate nucleotidyltransferase
-
-
-
-
nucleotidyltransferase, hexose 1-phosphate
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37278-26-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADPmannose + diphosphate
ATP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
GDP-glucose + diphosphate
GTP + D-glucose 1-phosphate
show the reaction diagram
-
best substrate
-
r
GDP-glucose + diphosphate
GTP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
r
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
reaction at about half the rate of GDPglucose
-
-
-
IDP-glucose + diphosphate
ITP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
IDP-glucose + diphosphate
ITP + D-glucose 1-phosphate
show the reaction diagram
-
reaction at 21% the rate of GDPglucose
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
reaction at 7% the rate of GDPglucose
-
-
-
UDPglucose + diphosphate
UTP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
UDPmannose + diphosphate
UTP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADPmannose + diphosphate
ATP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
GDP-glucose + diphosphate
GTP + D-glucose 1-phosphate
show the reaction diagram
-
best substrate
-
r
GDP-glucose + diphosphate
GTP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
r
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
GDPmannose + diphosphate
GTP + D-mannose 1-phosphate
show the reaction diagram
-
reaction at about half the rate of GDPglucose
-
-
-
IDP-glucose + diphosphate
ITP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
IDP-glucose + diphosphate
ITP + D-glucose 1-phosphate
show the reaction diagram
-
reaction at 21% the rate of GDPglucose
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
r
IDPmannose + diphosphate
ITP + D-mannose 1-phosphate
show the reaction diagram
-
reaction at 7% the rate of GDPglucose
-
-
-
UDPglucose + diphosphate
UTP + D-glucose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
UDPmannose + diphosphate
UTP + D-mannose 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
metal requirement can be met by Co2+
Mg2+
-
requirement, maximal activity at about 5 mM, maximal activity at about twice the diphosphate-concentration
Mg2+
-
required
Mn2+
-
metal requirement can be partially met by Mn2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
D-mannose 1-phosphate
-
strong
D-mannose 1-phosphate
-
-
GDPmannose
-
strong
GDPmannose
-
-
Zn2+
-
inhibits when added with Mg2+, Mn2+ or Co2+
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2.6
-
D-glucose 1-phosphate
-
pH 7.8, 25C
2.6
-
D-glucose 1-phosphate
-
-
0.082
-
diphosphate
-
pH 7.8, 25C
0.082
-
diphosphate
-
-
0.62
-
GDPglucose
-
pH 7.8, 25C
0.0001
-
GDPmannose
-
pH 7.8, 25C
0.0001
-
GDPmannose
-
-
2.6
-
GTP
-
pH 7.8, 25C
2.6
-
GTP
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.047
-
D-mannose 1-phosphate
-
pH 7.8, 25C
0.047
-
D-mannose 1-phosphate
-
-
0.00017
-
GDP-mannose
-
pH 7.8, 25C
0.00017
-
GDP-mannose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.38
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
9
-
pH 6.5: about 40% of maximal activity, pH 9.0: about half-maximal activity
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
6.5
-
t1/2: 60 days at 4C
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
-
15% loss of activity after 5 min, 33% loss of activity after 10 min
55
-
-
1 min, 57% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, at pH 6-6.5, t1/2: 60 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE