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Information on EC 2.7.4.B1 - yeast UMP kinase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P15700

for references in articles please use BRENDA:EC2.7.4.B1
preliminary BRENDA-supplied EC number
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P15700 not found.
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae
Synonyms
yeast ump kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
ATP:NMP phosphotransferase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 5-iodo-dUMP
ADP + 5-iodo-dUDP
show the reaction diagram
reaction occurs to a lesser extent
-
-
?
ATP + dGMP
ADP + dGDP
show the reaction diagram
reaction occurs to a lesser extent
-
-
?
ATP + dTMP
ADP + dTDP
show the reaction diagram
reaction occurs to a lesser extent
-
-
?
ATP + dUMP
ADP + dUDP
show the reaction diagram
-
-
-
?
ATP + GMP
ADP + GDP
show the reaction diagram
reaction occurs to a lesser extent
-
-
?
ATP + IMP
ADP + IDP
show the reaction diagram
reaction occurs to a lesser extent
-
-
?
ATP + UMP
ADP + UDP
show the reaction diagram
ATP + XMP
ADP + XDP
show the reaction diagram
reaction occurs to a lesser extent
-
-
?
CTP + UMP
CDP + UDP
show the reaction diagram
reaction rate is 10-14% of that with ATP or dATP
-
-
?
dATP + UMP
dADP + UDP
show the reaction diagram
-
-
-
?
dCTP + UMP
dCDP + UDP
show the reaction diagram
reaction rate is 30-50% of that with ATP or dATP
-
-
?
dGTP + UMP
dGDP + UDP
show the reaction diagram
reaction rate is 30-50% of that with ATP or dATP
-
-
?
dTTP + UMP
dTDP + UDP
show the reaction diagram
reaction rate is 30-50% of that with ATP or dATP
-
-
?
GTP + UMP
GDP + UDP
show the reaction diagram
reaction rate is 30-50% of that with ATP or dATP
-
-
?
UTP + UMP
UDP + UDP
show the reaction diagram
reaction rate is 10-14% of that with ATP or dATP
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + UMP
ADP + UDP
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
57% activity at 7.5 mM
Cd2+
no activity at 7.5 mM
Co2+
99% activity at 7.5 mM
Cu2+
11% activity at 7.5 mM
Fe2+
24% activity at 7.5 mM
Mg2+
100% activity at 7.5 mM
Mn2+
100% activity at 7.5 mM
Ni2+
84% activity at 7.5 mM
Zn2+
14% activity at 7.5 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithio-bis(2-nitrobenzoic acid)
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
monomer, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
enzyme activity decreases to 55%. In the presence of 1 mM UTP enzyme activity decreases to 73%. In the presence of 10% glycerol enzyme activity decreases to 77%. In the presence of 10% polyethylene glycol enzyme activity decreases to 95%. In the presence of 10 mM DTT enzyme activity decreases to 80%
60
enzyme activity decreases to 3%. In the presence of 1 mM UTP enzyme activity decreases to 10%. In the presence of 10% glycerol enzyme activity decreases to 14%. In the presence of 10% polyethylene glycol enzyme activity decreases to 34%. In the presence of 10 mM DTT enzyme activity decreases to 68%
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jong, A.; Yeh, Y.; Ma, J.J.
Characteristics, substrate analysis, and intracellular location of Saccharomyces cerevisiae UMP kinase
Arch. Biochem. Biophys.
304
197-204
1993
Saccharomyces cerevisiae (P15700)
Manually annotated by BRENDA team