We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
The taxonomic range for the selected organisms is: Bacillus anthracis The enzyme appears in selected viruses and cellular organisms
Synonyms
nm23-h1, nucleoside diphosphate kinase, ndp kinase, nm23-h2, ndpk-b, ndpk2, nucleoside diphosphokinase, ndk-1, nucleoside-diphosphate kinase, nucleoside diphosphate kinase a,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
nucleoside diphosphate kinase
-
kinase, nucleoside diphosphate (phosphorylating)
-
-
-
-
nucleoside 5'-diphosphate kinase
-
-
-
-
nucleoside diphosphate (UDP) kinase
-
-
-
-
nucleoside diphosphokinase
-
-
-
-
nucleotide phosphate kinase
-
-
-
-
uridine diphosphate kinase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
phospho group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + GDP
ADP + GTP
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
additional information
?
-
additional information
?
-
the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor
-
-
?
additional information
?
-
-
the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + NDP
ADP + NTP
-
-
-
?
additional information
?
-
additional information
?
-
the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor
-
-
?
additional information
?
-
-
the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mg2+
-
Mg2+
the activity is dependent on a metal ion (mainly Mg2+)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EDTA
complete inhibition at 10 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
22
assay at room temperature
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NDK_BACAN
148
0
16601
Swiss-Prot
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hexamer
exists as a hexamer that is composed of two SDS-stable trimers interacting in a back-to-back association
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
phosphoprotein
the presence of diethyl dicarbonate inhibits the autophosphorylation of Ndk
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hanging drop vapour-diffusion method, 0.002 ml protein solution consisting of 10 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM EDTA equilibrated against 0.5 ml reservoir solution consisting of 2.25 M ammonium formate and 0.1 M HEPES buffer, pH 7.25, X-ray diffraction structure determination at 2.0 A resolution
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
G19V
the mutation rigidifies the 43-63 loop in Ndk, using GTP as the phosphate donor a 300fold reduction in phosphotransferase activity is observed
H115Q
H115Q is incompetent in autophosphorylation, confirming the role of histidine as a phosphate acceptor
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ni-NTA column chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Misra, G.; Aggarwal, A.; Mittal, S.; Singh, Y.; Ramachandran, R.
Purification, crystallization and preliminary structural analysis of nucleoside diphosphate kinase from Bacillus anthracis
Acta Crystallogr. Sect. F
63
1084-1086
2007
Bacillus anthracis (Q81SV8), Bacillus anthracis
brenda
Misra, G.; Aggarwal, A.; Dube, D.; Zaman, M.S.; Singh, Y.; Ramachandran, R.
Crystal structure of the Bacillus anthracis nucleoside diphosphate kinase and its characterization reveals an enzyme adapted to perform under stress conditions
Proteins
76
496-506
2009
Bacillus anthracis (Q81SV8), Bacillus anthracis
brenda