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Information on EC 2.7.4.6 - nucleoside-diphosphate kinase and Organism(s) Bacillus anthracis and UniProt Accession Q81SV8

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IUBMB Comments
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
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This record set is specific for:
Bacillus anthracis
UNIPROT: Q81SV8
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Word Map
The taxonomic range for the selected organisms is: Bacillus anthracis
The enzyme appears in selected viruses and cellular organisms
Synonyms
nm23-h1, nucleoside diphosphate kinase, ndp kinase, nm23-h2, ndpk-b, ndpk2, nucleoside diphosphokinase, ndk-1, nucleoside-diphosphate kinase, nucleoside diphosphate kinase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleoside diphosphate kinase
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kinase, nucleoside diphosphate (phosphorylating)
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NDK
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nucleoside 5'-diphosphate kinase
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nucleoside diphosphate (UDP) kinase
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nucleoside diphosphokinase
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nucleotide phosphate kinase
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UDP kinase
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uridine diphosphate kinase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-51-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + GDP
ADP + GTP
show the reaction diagram
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?
ATP + NDP
ADP + NTP
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + NDP
ADP + NTP
show the reaction diagram
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
complete inhibition at 10 mM
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
assay at room temperature
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NDK_BACAN
148
0
16601
Swiss-Prot
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
exists as a hexamer that is composed of two SDS-stable trimers interacting in a back-to-back association
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
the presence of diethyl dicarbonate inhibits the autophosphorylation of Ndk
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour-diffusion method, 0.002 ml protein solution consisting of 10 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM EDTA equilibrated against 0.5 ml reservoir solution consisting of 2.25 M ammonium formate and 0.1 M HEPES buffer, pH 7.25, X-ray diffraction structure determination at 2.0 A resolution
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G19V
the mutation rigidifies the 43-63 loop in Ndk, using GTP as the phosphate donor a 300fold reduction in phosphotransferase activity is observed
H115Q
H115Q is incompetent in autophosphorylation, confirming the role of histidine as a phosphate acceptor
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Misra, G.; Aggarwal, A.; Mittal, S.; Singh, Y.; Ramachandran, R.
Purification, crystallization and preliminary structural analysis of nucleoside diphosphate kinase from Bacillus anthracis
Acta Crystallogr. Sect. F
63
1084-1086
2007
Bacillus anthracis (Q81SV8), Bacillus anthracis
Manually annotated by BRENDA team
Misra, G.; Aggarwal, A.; Dube, D.; Zaman, M.S.; Singh, Y.; Ramachandran, R.
Crystal structure of the Bacillus anthracis nucleoside diphosphate kinase and its characterization reveals an enzyme adapted to perform under stress conditions
Proteins
76
496-506
2009
Bacillus anthracis (Q81SV8), Bacillus anthracis
Manually annotated by BRENDA team