Information on EC 2.7.4.4 - nucleoside-phosphate kinase

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
2.7.4.4
-
RECOMMENDED NAME
GeneOntology No.
nucleoside-phosphate kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + nucleoside phosphate = ADP + nucleoside diphosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-phosphate phosphotransferase
Many nucleotides can act as acceptors; other nucleoside triphosphates can act instead of ATP.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
AMPK1
-
isoform
AMPK2
-
isoform
kinase, nucleoside monophosphate (phosphorylating)
-
-
-
-
NMP kinase
-
-
NMP-kinase
-
-
-
-
nucleoside monophosphate kinase
-
-
thymidylate kinase
-
-
TMPK
-
-
CAS REGISTRY NUMBER
COMMENTARY
9026-50-0
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + dAMP
ADP + dADP
show the reaction diagram
-
100% activity
-
-
?
ATP + GMP
ADP + GDP
show the reaction diagram
-
GTP, ITP, UTP are not donor substrates, IMP is not acceptor substrate
-
r
ATP + TMP
ADP + TDP
show the reaction diagram
-
-
-
-
?
ATP + UMP
ADP + UDP
show the reaction diagram
-
-
-
?
ATP + [2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
ADP + (2-((2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)ethyl)boronic phosphoric anhydride
show the reaction diagram
-
-
-
-
?
GTP + AMP
GDP + ADP
show the reaction diagram
-
GMP, CMP, UMP or IMP are not acceptor substrates
-
?
ITP + AMP
IDP + ADP
show the reaction diagram
-
IMP or GMP are not acceptor substrates
-
r
additional information
?
-
-
no activity with 3-(thymin-1-yl)propylboronic acid, (E)-3-(thymin-1-yl)prop-1-enylboronic acid, and (E)-1-(5'-deoxy-thymidin-5'-yl)-vinyl boronic acid
-
-
-
additional information
?
-
-
the enzyme shows almost no activity towards tenofovir and adefovir
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + TMP
ADP + TDP
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
half as effective as Mg2+ at 4 mM
Mg2+
-
activation
Mg2+
-
activity saturation at 4 mM
Mn2+
-
as effective as Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(E)-1-(5'-deoxy-thymidin-5'-yl)-vinyl boronic acid
-
about 30% inhibition at 1 mM
-
3-(thymin-1-yl)propylboronic acid
-
about 10% inhibition at 1 mM
-
[(E)-2-[(2R,3S,5R)-5-(6-amino-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl]ethenyl]boronic acid
-
about 15% inhibition at 1 mM
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
about 45% inhibition at 1 mM
[2-[(2R,3S,5R)-5-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl]ethyl]boronic acid
-
about 25% inhibition at 1 mM
[2-[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl]ethyl]boronic acid
-
about 55% inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
EDTA
-
variable degree of activation
glutathione
-
variable degree of activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.08
dAMP
-
isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
0.14
dAMP
-
isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
0.23
TMP
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
0.119
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
80
dAMP
-
isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
500
dAMP
-
isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
1.25
TMP
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
0.014
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1000
dAMP
-
isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
716
3000
dAMP
-
isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
716
5400
TMP
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
1020
0.139
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
28875
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
description of assay method
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 7.5
-
10% of the activity at pH 5.3
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1.3
-
inactivation in few min at 2C
642629
7
-
60C, stable for 2 min
642628
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60
-
2 min, considerably stable at pH 7
642628
80
-
completely inactivated at pH 7
642629
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C, 3 weeks, 75% of its activity lost
-
frozen, crude enzyme solution, indefinitely
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, using ammonium sulfate precipitation
-
partial
-