Information on EC 2.7.4.4 - nucleoside-phosphate kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
2.7.4.4
-
RECOMMENDED NAME
GeneOntology No.
nucleoside-phosphate kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + nucleoside phosphate = ADP + nucleoside diphosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Pyrimidine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-phosphate phosphotransferase
Many nucleotides can act as acceptors; other nucleoside triphosphates can act instead of ATP.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AMPK1
-
isoform
AMPK2
-
isoform
kinase, nucleoside monophosphate (phosphorylating)
-
-
-
-
NMP kinase
-
-
NMP-kinase
-
-
-
-
nucleoside monophosphate kinase
-
-
thymidylate kinase
-
-
TMPK
-
-
CAS REGISTRY NUMBER
COMMENTARY
9026-50-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + dAMP
ADP + dADP
show the reaction diagram
-
100% activity
-
-
?
ATP + GMP
ADP + GDP
show the reaction diagram
-
GTP, ITP, UTP are not donor substrates, IMP is not acceptor substrate
-
r
ATP + TMP
ADP + TDP
show the reaction diagram
-
-
-
-
?
ATP + UMP
ADP + UDP
show the reaction diagram
-
-
-
?
ATP + [2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
ADP + (2-((2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)ethyl)boronic phosphoric anhydride
show the reaction diagram
-
-
-
-
?
GTP + AMP
GDP + ADP
show the reaction diagram
-
GMP, CMP, UMP or IMP are not acceptor substrates
-
?
ITP + AMP
IDP + ADP
show the reaction diagram
-
IMP or GMP are not acceptor substrates
-
r
additional information
?
-
-
no activity with 3-(thymin-1-yl)propylboronic acid, (E)-3-(thymin-1-yl)prop-1-enylboronic acid, and (E)-1-(5'-deoxy-thymidin-5'-yl)-vinyl boronic acid
-
-
-
additional information
?
-
-
the enzyme shows almost no activity towards tenofovir and adefovir
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + TMP
ADP + TDP
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
half as effective as Mg2+ at 4 mM
Mg2+
-
activation
Mg2+
-
activity saturation at 4 mM
Mn2+
-
as effective as Mg2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(E)-1-(5'-deoxy-thymidin-5'-yl)-vinyl boronic acid
-
about 30% inhibition at 1 mM
-
3-(thymin-1-yl)propylboronic acid
-
about 10% inhibition at 1 mM
-
[(E)-2-[(2R,3S,5R)-5-(6-amino-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl]ethenyl]boronic acid
-
about 15% inhibition at 1 mM
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
about 45% inhibition at 1 mM
[2-[(2R,3S,5R)-5-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl]ethyl]boronic acid
-
about 25% inhibition at 1 mM
[2-[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl]ethyl]boronic acid
-
about 55% inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
variable degree of activation
glutathione
-
variable degree of activation
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.08
-
dAMP
-
isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
0.14
-
dAMP
-
isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
0.23
-
TMP
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
0.119
-
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
80
-
dAMP
-
isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
500
-
dAMP
-
isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
1.25
-
TMP
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
0.014
-
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1000
-
dAMP
-
isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
9416
3000
-
dAMP
-
isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37C
9416
5400
-
TMP
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
17221
0.139
-
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
-
in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37C
328742
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
description of assay method
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
7.5
-
10% of the activity at pH 5.3
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1.3
-
-
inactivation in few min at 2C
7
-
-
60C, stable for 2 min
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
2 min, considerably stable at pH 7
80
-
-
completely inactivated at pH 7
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-15C, 3 weeks, 75% of its activity lost
-
frozen, crude enzyme solution, indefinitely
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial, using ammonium sulfate precipitation
-
partial
-