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Information on EC 2.7.4.3 - adenylate kinase and Organism(s) Mus musculus and UniProt Accession Q9R0Y5
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2.7.4.3 adenylate kinaseIUBMB Comments
Inorganic triphosphate can also act as donor.
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Word Map
- 2.7.4.3
- phosphoenolpyruvate
- adp
- creatine
- hpr
- fructose
- nucleoside
-
permease
- streptococcus
-
phosphoenolpyruvate-dependent
- mannose
- 6-phosphate
-
catabolite
- deaminase
- lactose
- mannitol
- hexokinase
- mutans
- glucose-6-phosphate
-
camp-dependent
- aminoglycoside
- autophosphorylation
- phosphoglucomutase
-
phosphofructokinase
- kanamycin
- neomycin
- 6-phosphogluconate
-
phosphorelay
- 5'-nucleotidase
-
antiterminator
- mgatp
- 2-deoxyglucose
-
intermembrane
-
transphosphorylation
-
gamma-phosphate
- melibiose
- salivarius
- ganciclovir
- phosphocreatine
- nptii
-
diadenosine
-
diphosphohydrolase
-
gamma-32patp
-
p-loop
-
2.7.3.2
- dikinase
-
coarse-grained
- analysis
- phosvitin
- medicine
-
2,6-bisphosphate
- mucolipidosis
- phosphoenzyme
- diagnostics
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
phosphotransferase, adenylate kinase, myokinase, adenylate kinase 1, adenylate kinase 2, nonstructural protein 4b, cinap, adenylokinase, spadk, adenylate kinase isoenzyme 1, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
5'-AMP-kinase
-
-
-
-
adenylic kinase
-
-
-
-
adenylokinase
-
-
-
-
kinase, adenylate (phosphorylating)
-
-
-
-
kinase, myo- (phosphorylating)
-
-
-
-
myokinase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:AMP phosphotransferase
Inorganic triphosphate can also act as donor.
CAS REGISTRY NUMBER
COMMENTARY
9013-02-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ADP + ADP
?
-
facilitates storage and use of the high energy of the adenine nucleotides, involved in maintenance of equilibrium among adenine nucleotides and maintenance of energy charge, important to energy economy of living systems
-
-
r
AMP + H2O
ADP + phosphate
GMP can not be substituted for the AMP substrate
-
-
r
ATP + AMP
ADP + ADP
-
specificity for AMP-site is much more rigorous than for ATP-site
-
r
additional information
?
-
nucleotide-binding domain 1 cannot hydrolyze ATP
-
-
?
additional information
?
-
-
nucleotide-binding domain 1 cannot hydrolyze ATP
-
-
?
additional information
?
-
-
adenylte kinase-catalysed ADP production in the vicinity of K/ATP channels is involved in channel regulation
-
-
?
additional information
?
-
-
secretion of adenylate kinase 1 is required for extracellular ATP synthesis in myotubes
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ADP + ADP
?
-
facilitates storage and use of the high energy of the adenine nucleotides, involved in maintenance of equilibrium among adenine nucleotides and maintenance of energy charge, important to energy economy of living systems
-
-
r
additional information
?
-
-
adenylte kinase-catalysed ADP production in the vicinity of K/ATP channels is involved in channel regulation
-
-
?
additional information
?
-
-
secretion of adenylate kinase 1 is required for extracellular ATP synthesis in myotubes
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
adenosine 5'-(beta,gamma-imido)triphosphate tetralithium
-
non-metabolizable ATP analogue, inhibition of adenylate kinase abolishes the stimulatory effect of AMP on K/ATP channels
diadenosine polyphosphate
inhibits adenylate kinase activity of the nucleotide-binding domain 1 of CFTR
-
P1,P5-di(adenosine-5')pentaphosphate
-
inhibition of adenylate kinase prevents the stimulatory effect of AMP on K/ATP channels
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.4
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
expression is upregulated during late spermiogenesis when the flagellum is being assembled
-
expression level of adenylate kinase 1 and ATP synthase beta is strongly increased during myogenesis
mRNA is present at low but relatively equivalent levels in pachytene spermatocytes, round spermatids, and condensing spermatids
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
cytoplasmic adenylate kinase 1 is secreted from myotubes but not from myoblasts
-
additional information
additional information
localizes to the sodium dodecyl sulfate insoluble sperm tail fraction
-
additional information
localizes to the sodium dodecyl sulfate insoluble sperm tail fraction
-
additional information
-
localizes to the sodium dodecyl sulfate insoluble sperm tail fraction
-
additional information
localizes to the mitochondrial sheath in sperm midpiece
-
additional information
localizes to the mitochondrial sheath in sperm midpiece
-
additional information
-
localizes to the mitochondrial sheath in sperm midpiece
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
depletion of adenylate kinase 2 (AK2) by RNAi impairs adiponectin secretion in 3T3-L1 adipocytes, immunoglobulin M secretion in BCL1 cells, and the induction of the unfolded protein response during differentiation of both cell types. Depletion of AK2 results in changes in adipocyte energy homeostasis, but these effects do not detectably impair key adipocyte properties such as mitochondrial biogenesis and triglyceride storage
metabolism
-
adenylate kinase 2 links mitochondrial energy metabolism to the induction of the unfolded protein response
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). KAD1_MOUSE
194
0
21540
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
additional information
-
method for simultaneous detection of adenylate kinase isoforms directly on gel or nitrocellulose after separation by denaturing electrophoresis and electroblotting. Method allows for quantitative dection of enzyme activity from amny sources in both its reaction courses
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
G551D
mutation affects the ability of the nucleotide-binding domain 1 of CFTR to dimerize, has an exaggerated nonlinear phase compared with the wild type
additional information
additional information
-
in adenylate kinase knock-out mice, the stimulatory effect of AMP on K/ATP channels is much less pronounced, though not completely suppressed
additional information
-
knock out of the major isoform adenylate kinase 1 disrupts the synchrony between inorganic phosphate turnover at ATP-consuming sites and gamma-ATP exchange at ATP synthesis sites. This reduces energetic signal communication in the post-ischemic heart. Adenylate kinase 1 gene deletion blunts vascular adenylate kinase phosphotransfer, compromises the contractility-coronary flow relationship, and precipitates inadequate coronary reflow following ischemiareperfusion. Deficit in adenylate kinase activity abrogates AMP signal generation and reduces the vascular adenylate kinase/creatine kinase activity ratio essential for the response of metabolic sensors. The sarcolemma-associated splice variant adenylate kinase 1beta facilitates adenosine production. Adenosine treatment bypasseds adenylate kinase 1 deficiency and restores post-ischemic flow to wild-type levels
additional information
-
knock-down of adenylate kinase 1 results in block of synthesis of external ATP
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
nucleotide-binding domain 1 of CFTR purified to homogeneity by nickel ion affinity chromatography, followed by Sepharose S200 gel filtration and a second nickel affinity step
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into vector pET28b and expressed in Escherichia coli BL21DE3pLys cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
adenylate kinase 2 is markedly induced during adipocyte and B cell differentiation
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
method for simultaneous detection of adenylate kinase isoforms directly on gel or nitrocellulose after separation by denaturing electrophoresis and electroblotting. Method allows for quantitative dection of enzyme activity from amny sources in both its reaction courses
medicine
-
knock out of the major isoform adenylate kinase 1 disrupts the synchrony between inorganic phosphate turnover at ATP-consuming sites and gamma-ATP exchange at ATP synthesis sites. This reduces energetic signal communication in the post-ischemic heart. Adenylate kinase 1 gene deletion blunts vascular adenylate kinase phosphotransfer, compromises the contractility-coronary flow relationship, and precipitates inadequate coronary reflow following ischemiareperfusion. Deficit in adenylate kinase activity abrogates AMP signal generation and reduces the vascular adenylate kinase/creatine kinase activity ratio essential for the response of metabolic sensors. The sarcolemma-associated splice variant adenylate kinase 1beta facilitates adenosine production. Adenosine treatment bypasseds adenylate kinase 1 deficiency and restores post-ischemic flow to wild-type levels
additional information
additional information
-
isoenzyme AK1b mediated AMP-induced activation of recombinant ATP-sensitive potassium channels in presence of ATP
additional information
CFTR gating mechanism based on adenylate kinase activity
additional information
-
CFTR gating mechanism based on adenylate kinase activity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Noda, L.
Adenylate kinase
The Enzymes,3rd Ed. (Boyer,P. D. ,ed. )
8
279-305
1973
Bacillus subtilis, Bos taurus, Saccharomyces cerevisiae, Citrus limon, Blattidae, Oryctolagus cuniculus, Escherichia coli, Homo sapiens, Mus musculus, Physarum polycephalum, Rattus norvegicus, Sus scrofa, Thiobacillus denitrificans, Triticum aestivum
-
Janssen, E.; Kuiper, J.; Hodgson, D.; Zingman, L.V.; Alekseev, A.E.; Terzic, A.; Wieringa, B.
Two structurally distinct and spatially compartmentalized adenylate kinases are expressed from the AK1 gene in mouse brain
Mol. Cell. Biochem.
256/257
59-72
2004
Mus musculus
-
Cao, W.; Haig-Ladewig, L.; Gerton, G.L.; Moss, S.B.
Adenylate kinases 1 and 2 are part of the accessory structures in the mouse sperm flagellum
Biol. Reprod.
75
492-500
2006
Mus musculus (Q9R0Y5), Mus musculus (Q9WTP6), Mus musculus
Ravera, S.; Calzia, D.; Panfoli, I.; Pepe, I.M.; Morelli, A.
Simultaneous detection of molecular weight and activity of adenylate kinases after electrophoretic separation
Electrophoresis
28
291-300
2007
Mus musculus, Bos taurus (P00570)
Gross, C.H.; Abdul-Manan, N.; Fulghum, J.; Lippke, J.; Liu, X.; Prabhakar, P.; Brennan, D.; Willis, M.S.; Faerman, C.; Connelly, P.; Raybuck, S.; Moore, J.
Nucleotide-binding domains of cystic fibrosis transmembrane conductance regulator, an ABC transporter, catalyze adenylate kinase activity but not ATP hydrolysis
J. Biol. Chem.
281
4058-4068
2006
Homo sapiens (P13569), Homo sapiens, Mus musculus (P26361), Mus musculus
Schulze, D.U.; Duefer, M.; Wieringa, B.; Krippeit-Drews, P.; Drews, G.
An adenylate kinase is involved in KATP channel regulation of mouse pancreatic beta cells
Diabetologia
50
2126-2134
2007
Mus musculus
Choo, H.J.; Kim, B.W.; Kwon, O.B.; Lee, C.S.; Choi, J.S.; Ko, Y.G.
Secretion of adenylate kinase 1 is required for extracellular ATP synthesis in C2C12 myotubes
Exp. Mol. Med.
40
220-228
2008
Mus musculus
Dzeja, P.P.; Bast, P.; Pucar, D.; Wieringa, B.; Terzic, A.
Defective metabolic signaling in adenylate kinase AK1 gene knock-out hearts compromises post-ischemic coronary reflow
J. Biol. Chem.
282
31366-31372
2007
Mus musculus
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