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Information on EC 2.7.4.24 - diphosphoinositol-pentakisphosphate 1-kinase and Organism(s) Rattus norvegicus and UniProt Accession P0C644

for references in articles please use BRENDA:EC2.7.4.24
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IUBMB Comments
This enzyme is activated by osmotic shock . Ins(1,3,4,5,6)P5, 1D-myo-inositol diphosphate tetrakisphosphate and 1D-myo-inositol bisdiphosphate triphosphate are not substrates . The enzyme specifically phosphorylates the 1-position of the substrates .
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This record set is specific for:
Rattus norvegicus
UNIPROT: P0C644
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Synonyms
ppip5k, diphosphoinositol pentakisphosphate kinase, ip7 kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diphosphoinositol pentakisphosphate kinase
-
PP-InsP5 kinase
-
ATP:5-diphospho-1D-myo-inositol-pentakisphosphate phosphotransferase
-
-
-
-
diphospho-myo-inositol pentakisphosphate 5-kinase
-
-
-
-
diphosphoinositol pentakisphosphate kinase
-
-
-
-
kinase (phosphorylating), diphosphoinositol 1,2,3,4,5-pentakisphosphate 5-
-
-
-
-
PP-InsP5 kinase
-
-
-
-
PP-IP5 kinase
-
-
-
-
PPIP5K
-
-
-
-
PPIP5K1
-
-
-
-
PPIP5K2
-
-
-
-
VIP1
-
-
-
-
VIP2
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:1D-myo-inositol-5-diphosphate-pentakisphosphate 1-phosphotransferase
This enzyme is activated by osmotic shock [4]. Ins(1,3,4,5,6)P5, 1D-myo-inositol diphosphate tetrakisphosphate and 1D-myo-inositol bisdiphosphate triphosphate are not substrates [4]. The enzyme specifically phosphorylates the 1-position of the substrates [6].
CAS REGISTRY NUMBER
COMMENTARY hide
188929-01-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1D-myo-inositol 5-diphosphate pentakisphosphate
ADP + 1D-myo-inositol bisdiphosphate tetrakisphosphate
show the reaction diagram
ADP + 1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate
ATP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate
show the reaction diagram
-
-
-
-
?
ADP + diphosphoinositol pentakisphosphate
ATP + ?
show the reaction diagram
-
enzyme has ATP synthase activity
-
-
?
ATP + 5-diphospho-1D-myo-inositol-pentakisphosphate
ADP + bis(diphospho)-1D-myo-inositol tetrakisphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 1D-myo-inositol 5-diphosphate pentakisphosphate
ADP + 1D-myo-inositol bisdiphosphate tetrakisphosphate
show the reaction diagram
(PP)2-InsP4 synthesis pathway, overview
-
-
?
ATP + 5-diphospho-1D-myo-inositol-pentakisphosphate
ADP + bis(diphospho)-1D-myo-inositol tetrakisphosphate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
F-
-
50% inhibition at 0.03 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.94
1D-myo-inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate
-
pH 6.8, 37°C
1.39
ADP
-
pH 6.8, 37°C
1.89
ATP
-
pH 6.8, 37°C
0.7
diphosphoinositol pentakisphosphate
-
pH 6.8, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.5
-
half maximal acitvity at pH 5.5 and pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 40
-
70% activity at 25°C and 40°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
VIP1_RAT
1434
0
159619
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
160000
about, isozyme PPIP5K1, mass spectrometry
200000
sucrose density gradient centrifugation
400000
gel filtration
56000
-
SDS-PAGE
60000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
the enzyme contains a putative ATP-grasp kinase domain
monomer
-
1 * 56000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
complete loss of activity within 18 h
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% loss of activity in 20% glycerol after 1 week
-
-70°C, no loss of activity in 20% glycerol for 3 months
-
4°C, complete loss of activity within 18 h
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native PPIP5K1 from rat brain by anion exchange and heparin affinity chromatography, followed by nickel affinity chromatography and sucrose gradient centrifugation
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Voglmaier, S.M.; Bembenek, M.E.; Kaplin, A.I.; Dorman, G.; Olszewski, J.D.; Prestwich, G.D.; Snyder, S.H.
Purified inositol hexakisphosphate kinase is an ATP synthase: diphosphoinositol pentakisphosphate as a high-energy phosphate donor
Proc. Natl. Acad. Sci. USA
93
4305-4310
1996
Rattus norvegicus
Manually annotated by BRENDA team
Huang, C.F.; Voglmaier, S.M.; Bembenek, M.E.; Saiardi, A.; Snyder, S.H.
Identification and purification of diphosphoinositol pentakisphosphate kinase, which synthesizes the inositol pyrophosphate bis(diphospho)inositol tetrakisphosphate
Biochemistry
37
14998-15004
1998
Rattus norvegicus
Manually annotated by BRENDA team
Shears, S.B.; Ali, N.; Craxton, A.; Bembenek, M.E.
Synthesis and metabolism of bis-diphosphoinositol tetrakisphosphate in vitro and in vivo
J. Biol. Chem.
270
10489-10497
1995
Rattus norvegicus
Manually annotated by BRENDA team
Choi, J.H.; Williams, J.; Cho, J.; Falck, J.R.; Shears, S.B.
Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress
J. Biol. Chem.
282
30763-30775
2007
Rattus norvegicus (P0C644), Homo sapiens (Q6PFW1), Homo sapiens
Manually annotated by BRENDA team