Information on EC 2.7.4.23 - ribose 1,5-bisphosphate phosphokinase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.7.4.23
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RECOMMENDED NAME
GeneOntology No.
ribose 1,5-bisphosphate phosphokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
aminomethylphosphonate degradation
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glyphosate degradation III
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PRPP biosynthesis II
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Pentose phosphate pathway
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SYSTEMATIC NAME
IUBMB Comments
ATP:ribose-1,5-bisphosphate phosphotransferase
This enzyme, found in NAD supression mutants of Escherichia coli, synthesizes 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) without the participation of EC 2.7.6.1, ribose-phosphate diphosphokinase. Ribose, ribose 1-phosphate and ribose 5-phosphate are not substrates, and GTP cannot act as a phosphate donor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
K-12 strains; phnN
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + ribose 1,5-bisphosphate
5-phospho-alpha-D-ribose 1-diphosphate + ADP
show the reaction diagram
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
acitivity very low in a crude extract of strain HO1088/pTR553 before addition of IPTG
0.03
acitivity in a crude extract of strain HO1088/pHO500 after addition of IPTG
0.12
acitivity in a crude extract of strain HO1088/pTR553 after addition of IPTG
8.3
purified PhnN protein
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21410
calculated from the deduced nucleotide sequence of the allele specifying the histidine-tailed polypeptide
23500
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification of histidine-tailed ribose 1,5-bisphosphokinase, centrifugation, ultrasonic treatment, application to Co2+-containing Talon metal affinity resin, purified almost to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
into pSK50-delta A2 and then subcloned into pCAH56 and into pAH126
Show AA Sequence (2655 entries)
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