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Information on EC 2.7.4.16 - thiamine-phosphate kinase and Organism(s) Aquifex aeolicus and UniProt Accession O67883

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Aquifex aeolicus
UNIPROT: O67883 not found.
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Word Map
The taxonomic range for the selected organisms is: Aquifex aeolicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
thiamin pyrophosphokinase, thiamine monophosphate kinase, thiamin phosphate kinase, thiamin-monophosphate kinase, thiamine-phosphate kinase, thiamin monophosphate kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
thiamin monophosphate kinase
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ATP:thiamin-phosphate phosphotransferase
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kinase, thiamin monophosphate (phosphorylating)
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thiamin monophosphatase
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thiamin monophosphate kinase
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thiamin monophosphokinase
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thiamin phosphate kinase
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thiamin-monophosphate kinase
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thiamin-phosphate kinase
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thiamine monophosphate kinase
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thiamine monophosphokinase
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additional information
ThiL is a member of a small ATP binding superfamily
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + thiamine phosphate = ADP + thiamine diphosphate
show the reaction diagram
Aquifex aeolicus ThiL utilizes a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate, reaction mechanism, overview
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:thiamine-phosphate phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9068-23-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + thiamine phosphate
ADP + thiamine diphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + thiamine phosphate
ADP + thiamine diphosphate
show the reaction diagram
ThiL catalyzes the ATP-dependent phosphorylation of thiamin monophosphate to form thiamin diphosphate, the active form of vitamin B1
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Uniprot
Manually annotated by BRENDA team
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme in complex with nonhydrolyzable AMP-PCP and TMP, or with the reaction products ADP and TPP, ThiL is incubated with 4 mM AMP-PCP or ATP and 4 mM MgCl2 on ice for 1 h prior to crystallization experiments, optimized conditions for the ThiL binary complexes include 100 mM HEPES, pH 7.4, 6-11% isopropyl alcohol, 200 mM NaCl, and 4-6% 2-methyl-2,4-pentanediol, 100 mM CaCl2 is used as an additive in a 1:9 ratio with the reservoir solution, TMP is soaked into the co-crystallized ThiL and AMP-PCP crystals by transferring the crystals to a solution containing the mother liquor and 20 mM TMP and allowing the crystals to soak for 5 min before being frozen. To obtain ThiL ADP-TPP crystals, ThiL is incubated on ice with 4 mM ADP, 4 mM TPP, and 4 mM MgCl2, optimized conditions for the product complex include 100 mM imidazole, pH 8.0, 7-10% PEG 8000, and 100-250 mM Ca(C2H3O2)2, 3-7 days, cryoprotection by 15% ethylene glycol, X-ray diffraction structure determination and analysis at 1.5-2.6 A resolution
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
McCulloch, K.M.; Kinsland, C.; Begley, T.P.; Ealick, S.E.
Structural studies of thiamin monophosphate kinase in complex with substrates and products
Biochemistry
47
3810-3821
2008
Aquifex aeolicus (O67883), Aquifex aeolicus
Manually annotated by BRENDA team