Information on EC 2.7.4.15 - thiamine-diphosphate kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.7.4.15
-
RECOMMENDED NAME
GeneOntology No.
thiamine-diphosphate kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + thiamine diphosphate = ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
thiamin triphosphate metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:thiamine-diphosphate phosphotransferase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ATP:thiamin-diphosphate phosphotransferase
-
-
-
-
kinase (phosphorylating), thiamin diphosphate
-
-
-
-
kinase, thiamin diphosphate (phosphorylating)
-
-
-
-
protein bound thiamin diphosphate:ATP phosphoryltransferase
-
-
-
-
TDP kinase
-
-
-
-
thiamin diphosphate kinase
-
-
-
-
thiamin diphosphate phosphotransferase
-
-
-
-
thiamin pyrophosphate kinase
-
-
-
-
thiamin-diphosphate kinase
-
-
-
-
thiamine diphosphate kinase
-
-
-
-
thiamine diphosphate kinase
-
-
thiamine pyrophosphate-ATP phosphoryltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9075-79-0
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
BL21 strain and heat-sensitive CV2, CGSC 4682 strain
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
r
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
CTP + thiamine diphosphate
CDP + thiamine triphosphate
show the reaction diagram
-
6% of the activity with ATP
-
-
?
additional information
?
-
-
enzymatic synthesis of thiamine triphosphate in Escherichia coli
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
-
about 50% of the activation with Mg2+
Fe2+
-
30 mM FeCl2, maximal activation of approximately 30%
Fe3+
-
30 mM FeCl32, maximal activation of approximately 30%
Mg2+
-
required
Mg2+
-
required
Mg2+
-
required
Mn2+
-
about 50% of the activation with Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
HgCl2
-
10 mM, complete inhibition
NEM
-
10 mM, 64% inhibition
PCMB
-
0.5 mM, 79.5% inhibition
PCMB
-
10 mM, complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Creatine
-
the enzyme is dependent on creatine
additional information
-
a low molecular weight cofactor is required
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
ATP
-
-
0.6
ATP
-
pH 7.4, 37°C
1.1
thiamine diphosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
thiamine triphoshate accumulation in response to amino acid starvation, controverse mechanism of synthesis dicussed, adenylate kinase-independent accumulation of thiamine triphosphate, heat-sensitive CV2 strain of Escherichia coli accumulates thiamine triphosphate despite full inactivation of adenylate cyclase and subsequent decrease of cellular ATP, ATP-dependent mechanisms for thiamine triphosphate synthesis not favoured since large accumulation also observed during energy stress where ATP is low
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
-
in Tris-HCl buffer slightly higher activity than in phosphate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.1
-
isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
primarily localized in
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
103000
-
-
645165
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE