Information on EC 2.7.4.15 - thiamine-diphosphate kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.7.4.15
-
RECOMMENDED NAME
GeneOntology No.
thiamine-diphosphate kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + thiamine diphosphate = ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
-
thiamin triphosphate metabolism
-
Thiamine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
ATP:thiamine-diphosphate phosphotransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
ATP:thiamin-diphosphate phosphotransferase
-
-
-
-
kinase (phosphorylating), thiamin diphosphate
-
-
-
-
kinase, thiamin diphosphate (phosphorylating)
-
-
-
-
protein bound thiamin diphosphate:ATP phosphoryltransferase
-
-
-
-
TDP kinase
-
-
-
-
thiamin diphosphate kinase
-
-
-
-
thiamin diphosphate phosphotransferase
-
-
-
-
thiamin pyrophosphate kinase
-
-
-
-
thiamin-diphosphate kinase
-
-
-
-
thiamine diphosphate kinase
-
-
-
-
thiamine diphosphate kinase
-
-
thiamine pyrophosphate-ATP phosphoryltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9075-79-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
BL21 strain and heat-sensitive CV2, CGSC 4682 strain
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
r
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
show the reaction diagram
-
-
-
-
?
CTP + thiamine diphosphate
CDP + thiamine triphosphate
show the reaction diagram
-
6% of the activity with ATP
-
-
?
additional information
?
-
-
enzymatic synthesis of thiamine triphosphate in Escherichia coli
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
about 50% of the activation with Mg2+
Fe2+
-
30 mM FeCl2, maximal activation of approximately 30%
Fe3+
-
30 mM FeCl32, maximal activation of approximately 30%
Mg2+
-
required
Mg2+
-
required
Mg2+
-
required
Mn2+
-
about 50% of the activation with Mg2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
HgCl2
-
10 mM, complete inhibition
NEM
-
10 mM, 64% inhibition
PCMB
-
0.5 mM, 79.5% inhibition
PCMB
-
10 mM, complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Creatine
-
the enzyme is dependent on creatine
additional information
-
a low molecular weight cofactor is required
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.6
-
ATP
-
pH 7.4, 37°C
1.1
-
thiamine diphosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
thiamine triphoshate accumulation in response to amino acid starvation, controverse mechanism of synthesis dicussed, adenylate kinase-independent accumulation of thiamine triphosphate, heat-sensitive CV2 strain of Escherichia coli accumulates thiamine triphosphate despite full inactivation of adenylate cyclase and subsequent decrease of cellular ATP, ATP-dependent mechanisms for thiamine triphosphate synthesis not favoured since large accumulation also observed during energy stress where ATP is low
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
-
in Tris-HCl buffer slightly higher activity than in phosphate buffer
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.1
-
-
isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
primarily localized in
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
103000
-
-
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE