Information on EC 2.7.3.5 - lombricine kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Coelomata

EC NUMBER
COMMENTARY
2.7.3.5
-
RECOMMENDED NAME
GeneOntology No.
lombricine kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + lombricine = ADP + N-phospholombricine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Glycine, serine and threonine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
ATP:lombricine N-phosphotransferase
Also acts on methylated lombricines such as thalassemine; the specificity varies with the source species.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
guanidinethylphosphoserine kinase
-
-
-
-
kinase (phosphorylating), lombricine
-
-
-
-
kinase, lombricine (phosphorylating)
-
-
-
-
LK
-
-
-
-
LK
-
cytoplasmic enzyme
MiLK
-
mitochondrial enzyme
CAS REGISTRY NUMBER
COMMENTARY
9026-53-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
California blackworm
UniProt
Manually annotated by BRENDA team
Megascolides cameroni
-
-
-
Manually annotated by BRENDA team
Stylaria sp.
-
UniProt
Manually annotated by BRENDA team
-
C8CG36
UniProt
Manually annotated by BRENDA team
sludge worm
C8CG36
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-lombricine
ADP + phospholombricine
show the reaction diagram
Megascolides cameroni
-
-
-
-
r
ATP + D-lombricine
ADP + phospholombricine
show the reaction diagram
-
D-isomer, 100%, and L-isomer, 176%, are reactive
-
-
?
ATP + guanidinoethyl phosphate
ADP + N-phospho-guanidinoethyl phosphate
show the reaction diagram
-
15% of the activity with D-lombricine
-
-
?
ATP + L-lombricine
ADP + phospholombricine
show the reaction diagram
Megascolides cameroni
-
-
-
-
r
ATP + L-lombricine
ADP + phospholombricine
show the reaction diagram
-
D-isomer, 100%, and L-isomer, 176%, are reactive
-
-
?
ATP + L-thalassemine
ADP + N-L-thalassemine phosphate
show the reaction diagram
-
i.e. guanidinoethylphospho-O-(alpha-N,N-dimethyl)serine, 88% of the activity with D-lombricine
-
-
-
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
C8CG36, -
-
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
C8CG36, -
-
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
B0FYX1
-
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
I0E0A8
-
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
Stylaria sp.
I0E0A7
-
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
I0E0A8
-
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
I0E0A7
-
-
-
?
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
-
-
-
?
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
-
-
-
?
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
Q8T6T7, -
-
-
-
-
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
Megascolides cameroni
-
i.e. guanidinoethylphospho-O-serine
-
r
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
D-isomer, 100%, and L-isomer, 176%, are reactive
-
-
?
ATP + taurocyamine
ADP + N-taurocyamine phosphate
show the reaction diagram
-
44% of the activity with D-lombricine
-
-
?
dADP + phospholombricine
dATP + lombricine
show the reaction diagram
Megascolides cameroni
-
at 16% of the activity with ADP
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
Megascolides cameroni
-
5 mM, slight activation in both directions
Co2+
Megascolides cameroni
-
5 mM, slight activation in both directions
Mg2+
-
enzyme contains one binding site for MgADP-
Mg2+
Megascolides cameroni
-
5 mM, activation in both directions
Mn2+
Megascolides cameroni
-
5 mM, activation in both directions
additional information
Megascolides cameroni
-
not activated by Ni2+, Cu2+, Fe2+, Fe3+, Al3+, Sn2+, Ba2+, Cd2+, Zn2+, Be2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,4-Dinitrofluorobenzene
-
-
NEM
Megascolides cameroni
-
0.3 mM, complete inhibition
o-Iodosobenzoate
-
-
o-Iodosobenzoate
Megascolides cameroni
-
0.3 mM, complete inhibition
p-hydroxymercuribenzoate
Megascolides cameroni
-
0.0001 mM, complete inhibition
Phenyl iodoacetate
-
-
Phenyl iodoacetate
Megascolides cameroni
-
1 mM, complete inhibition
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
9.1
-
D-Lombricine
Megascolides cameroni
-
pH 8.6, 30C
11.7
-
L-lombricine
Megascolides cameroni
-
pH 8.6, 30C
7.4
-
phospholombricine
Megascolides cameroni
-
pH 8.6, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
12.2
-
-
25C
31
-
Megascolides cameroni
-
D-lombricine
49
-
Megascolides cameroni
-
L-lombricine
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.2
-
Megascolides cameroni
-
synthesis of ATP
8.6
-
Megascolides cameroni
-
phosphorylation of lombricine
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
8
Megascolides cameroni
-
pH 6.5: about 85% of maximal activity, pH 8.0: about 50% of maximal activity, synthesis of ATP
7.5
9.5
Megascolides cameroni
-
pH 7.5: about 65% of maximal activity, pH 9.5: about 45% of maximal activity, phosphorylation of lombricine
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.87
-
-
-
7.6
-
B0FYX1
calculated from amino acid sequence
7.7
-
C8CG36, -
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Megascolides cameroni
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
74200
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 40941, calculation from nucleotide sequence
?
B0FYX1
x * 41437, calculated from amino acid sequence
?
C8CG36, -
x * 41649, calculated from amino acid sequence
dimer
-
two non-identical subunits, Sepharose-mercurial chromatography
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
substrate-free enzyme or in complex with ADP, vapor diffusion method, using 15 mM bis-Tris, 0.2 M NaNO3, 1 mM dithiothreitol, and 20% (w/v) PEG 3350MME, pH 6.8
-
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
freeze-drying of preparations in non-volatile buffers causes almost 40% loss of activity. Freezing and thawing at pH 8.6 in N-ethylmorpholine-hydrochloric acid buffer produces complete inactivation
Megascolides cameroni
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
0C, 0.033 M phosphate buffer, pH 7, or 0.05 M Tris-HCl buffer, pH 7.5, or 0.01 M glycylglycine buffer, pH 7, several weeks stable, or in 75% saturated ammonium sulfate solution, pH 8 stable for several months
-
2C, pH 7.5, 50% v/v glycerol, several months stable
Megascolides cameroni
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Megascolides cameroni
-
DEAE column chromatography, ADP-agarose column chromatography, and Superdex 200 gel filtration
-
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli TOP 10 cells
-, I0E0A8
expressed in Escherichia coli TOP 10 cells
Stylaria sp.
I0E0A7
expressed in Escherichia coli
-
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
K95Y
-
the mutation produces a dramatic change in guanidine substrate specificity, resulting in a shift of substrate specificity from lombricine to taurocyamine, the K95Y mutant functions not as lombricine kinase but as taurocyamine kinase