Information on EC 2.7.3.5 - lombricine kinase

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The expected taxonomic range for this enzyme is: Coelomata

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
2.7.3.5
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
lombricine kinase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + lombricine = ADP + N-phospholombricine
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycine, serine and threonine metabolism
-
-
SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
ATP:lombricine N-phosphotransferase
Also acts on methylated lombricines such as thalassemine; the specificity varies with the source species.
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
guanidinethylphosphoserine kinase
-
-
-
-
kinase (phosphorylating), lombricine
-
-
-
-
kinase, lombricine (phosphorylating)
-
-
-
-
LK
-
-
-
-
LK
-
cytoplasmic enzyme
MiLK
-
mitochondrial enzyme
SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
9026-53-3
-
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
California blackworm
UniProt
Manually annotated by BRENDA team
Megascolides cameroni
-
-
-
Manually annotated by BRENDA team
Stylaria sp.
-
UniProt
Manually annotated by BRENDA team
sludge worm
UniProt
Manually annotated by BRENDA team
SplaateGeneral_Information, Organism, Commentary, Literature
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-lombricine
ADP + phospholombricine
show the reaction diagram
Megascolides cameroni
-
-
-
r
ATP + D-lombricine
ADP + phospholombricine
show the reaction diagram
-
D-isomer, 100%, and L-isomer, 176%, are reactive
-
?
ATP + guanidinoethyl phosphate
ADP + N-phospho-guanidinoethyl phosphate
show the reaction diagram
-
15% of the activity with D-lombricine
-
?
ATP + L-lombricine
ADP + phospholombricine
show the reaction diagram
Megascolides cameroni
-
-
-
r
ATP + L-lombricine
ADP + phospholombricine
show the reaction diagram
-
D-isomer, 100%, and L-isomer, 176%, are reactive
-
?
ATP + L-thalassemine
ADP + N-L-thalassemine phosphate
show the reaction diagram
-
i.e. guanidinoethylphospho-O-(alpha-N,N-dimethyl)serine, 88% of the activity with D-lombricine
-
-
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
Stylaria sp.
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
?
ATP + lombricine
ADP + N-phospholombricine
show the reaction diagram
-
-
?
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
-
-
?
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
-
-
?
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
-
-
?
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
-
-
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
Megascolides cameroni
-
i.e. guanidinoethylphospho-O-serine
-
r
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
D-isomer, 100%, and L-isomer, 176%, are reactive
-
?
ATP + taurocyamine
ADP + N-taurocyamine phosphate
show the reaction diagram
-
44% of the activity with D-lombricine
-
?
dADP + phospholombricine
dATP + lombricine
show the reaction diagram
Megascolides cameroni
-
at 16% of the activity with ADP
-
?
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + lombricine
ADP + phospholombricine
show the reaction diagram
-
-
-
?
SplaateCofactor,Organism,Commentary,Literature,Filename
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SplaateMetals_Ions,Organism,Commentary, Literature
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Megascolides cameroni
-
5 mM, slight activation in both directions
Co2+
Megascolides cameroni
-
5 mM, slight activation in both directions
Mg2+
-
enzyme contains one binding site for MgADP-
Mg2+
Megascolides cameroni
-
5 mM, activation in both directions
Mn2+
Megascolides cameroni
-
5 mM, activation in both directions
additional information
Megascolides cameroni
-
not activated by Ni2+, Cu2+, Fe2+, Fe3+, Al3+, Sn2+, Ba2+, Cd2+, Zn2+, Be2+
SplaateInhibitors, Organism, Commentary, Literature,Filename
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2,4-Dinitrofluorobenzene
-
-
NEM
Megascolides cameroni
-
0.3 mM, complete inhibition
o-Iodosobenzoate
-
-
o-Iodosobenzoate
Megascolides cameroni
-
0.3 mM, complete inhibition
p-hydroxymercuribenzoate
Megascolides cameroni
-
0.0001 mM, complete inhibition
Phenyl iodoacetate
-
-
Phenyl iodoacetate
Megascolides cameroni
-
1 mM, complete inhibition
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
9.1
D-Lombricine
Megascolides cameroni
-
pH 8.6, 30C
11.7
L-lombricine
Megascolides cameroni
-
pH 8.6, 30C
7.4
phospholombricine
Megascolides cameroni
-
pH 8.6, 30C
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
12.2
-
25C
31
Megascolides cameroni
-
D-lombricine
49
Megascolides cameroni
-
L-lombricine
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.2
Megascolides cameroni
-
synthesis of ATP
8.6
Megascolides cameroni
-
phosphorylation of lombricine
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 8
Megascolides cameroni
-
pH 6.5: about 85% of maximal activity, pH 8.0: about 50% of maximal activity, synthesis of ATP
7.5 - 9.5
Megascolides cameroni
-
pH 7.5: about 65% of maximal activity, pH 9.5: about 45% of maximal activity, phosphorylation of lombricine
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.6
calculated from amino acid sequence
7.7
calculated from amino acid sequence
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Megascolides cameroni
-
-
Manually annotated by BRENDA team
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
74200
-
gel filtration
642486
80000
-
-
642489
SplaateSubunits, Organism, Commentary, Literature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 40941, calculation from nucleotide sequence
?
x * 41437, calculated from amino acid sequence
?
x * 41649, calculated from amino acid sequence
dimer
-
two non-identical subunits, Sepharose-mercurial chromatography
SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
substrate-free enzyme or in complex with ADP, vapor diffusion method, using 15 mM bis-Tris, 0.2 M NaNO3, 1 mM dithiothreitol, and 20% (w/v) PEG 3350MME, pH 6.8
-
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freeze-drying of preparations in non-volatile buffers causes almost 40% loss of activity. Freezing and thawing at pH 8.6 in N-ethylmorpholine-hydrochloric acid buffer produces complete inactivation
Megascolides cameroni
-
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, 0.033 M phosphate buffer, pH 7, or 0.05 M Tris-HCl buffer, pH 7.5, or 0.01 M glycylglycine buffer, pH 7, several weeks stable, or in 75% saturated ammonium sulfate solution, pH 8 stable for several months
-
2C, pH 7.5, 50% v/v glycerol, several months stable
Megascolides cameroni
-
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
Megascolides cameroni
-
DEAE column chromatography, ADP-agarose column chromatography, and Superdex 200 gel filtration
-
recombinant enzyme
-
SplaateCommentary, Organism, Literature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli TOP 10 cells
expressed in Escherichia coli TOP 10 cells
Stylaria sp.
expressed in Escherichia coli
-
expression in Escherichia coli
-
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
K95Y
-
the mutation produces a dramatic change in guanidine substrate specificity, resulting in a shift of substrate specificity from lombricine to taurocyamine, the K95Y mutant functions not as lombricine kinase but as taurocyamine kinase
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature