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Information on EC 2.7.3.4 - taurocyamine kinase and Organism(s) Schistosoma mansoni and UniProt Accession P16641

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Schistosoma mansoni
UNIPROT: P16641 not found.
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Word Map
The taxonomic range for the selected organisms is: Schistosoma mansoni
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
phosphagen kinase, taurocyamine kinase, guanidino kinase, taurocyamine phosphotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
guanidino kinase
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ATP:taurocyamine phosphotransferase
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-
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kinase (phosphorylating), taurocyamine
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-
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kinase, taurocyamine (phosphorylating)
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taurocyamine phosphotransferase
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TPK
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-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + taurocyamine = ADP + N-phosphotaurocyamine
show the reaction diagram
an arginine residue of the phosphagen specificity loop is crucial for substrate specificity, mechanism and structure-function relationship, overview
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
ATP:taurocyamine N-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-72-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + taurocyamine
ADP + N-phosphotaurocyamine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + taurocyamine
ADP + N-phosphotaurocyamine
show the reaction diagram
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-
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
dependent on
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the phosphagen kinase (PK) family. Schistosoma mansoni taurocyamine SmTK is derived from gene duplication, as are all known trematode taurocyamine kinases
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KTRC_SCHMA
746
0
83891
Swiss-Prot
Secretory Pathway (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant wild-type and mutant enzymes complexed with taurocyamine or L-arginine, sitting drop vapor diffusion method, mixing of 5 mg/ml protein in 20mM Tris-HCl, pH 8.5, with reservoir solution, containing 200mM diammonium tartrate, pH 5.4, 20% w/v PEG 3350, 20% v/v ethylene glycol, in a 2:1 or 1:1 protein/solution volume ratio, 2 weeks, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution leads to a small angle x-ray scattering model of SmTK-TSA in solution with two closed active sites, molecular replacement. The SmTK crystal is soaked with the dead end transition state analogue components taurocyamine-NO3 2-MgADP
sitting-drop vapour-diffusion, 100 mM MES, pH 6.5, 25%(w/v) PEG 3000, 290 K, unit-cell parameters a = 52.7, b = 122.1, c = 63.2 A , beta = 108.5°, space group P21, 2.8 A resolution on ESRF beamline ID29
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C268S
site-directed mutagenesis
C268S/C631S
site-directed mutagenesis
C631S
site-directed mutagenesis
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatogrphy, concentrated to 10 mg ml-1 by lyophilization and resolubilized in MilliQ water, the purity is verified by the presence of a single band on overloaded Coomassie Blue-stained SDS-PAGE
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by Blue Sepharose affinity and anion exchange chromatography, followed by ultrafiltration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
Schistosoma mansoni is a trematode flatworm that has been identified as the aetiological agent of schistosomiasis, a disease that affects about 200 million people worldwide
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Awama, A.M.; Paracuellos, P.; Laurent, S.; Dissous, C.; Marcillat, O.; Gouet, P.
Crystallization and x-ray analysis of the Schistosoma mansoni guanidino kinase
Acta Crystallogr. Sect. F
F64
854-857
2008
Schistosoma mansoni (P16641), Schistosoma mansoni
Manually annotated by BRENDA team
Merceron, R.; Awama, A.M.; Montserret, R.; Marcillat, O.; Gouet, P.
The substrate-free and -bound crystal structures of the duplicated taurocyamine kinase from the human parasite Schistosoma mansoni
J. Biol. Chem.
290
12951-12963
2015
Schistosoma mansoni (P16641), Schistosoma mansoni
Manually annotated by BRENDA team