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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
glycocyamine kinase, guanidinoacetate kinase,
more
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kinase, guanidinoacetate (phosphorylating)
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glycocyamine kinase
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glycocyamine kinase
Namalycastis sp.
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glycocyamine kinase
Namalycastis sp.
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ATP + guanidinoacetate = ADP + phosphoguanidinoacetate
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-
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phospho group transfer
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-
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ATP:guanidinoacetate N-phosphotransferase
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ADP + phosphocreatine
ATP + ?
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poor substrate
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-
?
ADP + phosphoguanidinoacetate
ATP + guanidinoacetate
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-
-
?
ATP + creatine
ADP + phosphocreatine
Namalycastis sp.
0.5% of the activity with glycocyamine
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-
r
ATP + glycocyamine
ADP + phosphoglycocyamine
Namalycastis sp.
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-
-
r
ATP + guanidine
ADP + phosphoguanidine
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
ATP + guanidine
ADP + phosphoguanidine
-
-
-
r
ATP + guanidine
ADP + phosphoguanidine
-
-
-
-
?
ATP + guanidine
ADP + phosphoguanidine
Polycelis cornuta
-
-
-
-
?
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
-
-
-
-
?
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
Namalycastis sp.
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-
-
r
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
-
-
-
-
?
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
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not: arginine, creatine, lombricine
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r
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
Perinereis sp.
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-
-
?
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
Perinereis sp.
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specific for guanidinoacetate, ITP 2%, GTP 0.5% of ATP activity
-
?
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
Polycelis cornuta
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-
-
-
?
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ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
Namalycastis sp.
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-
-
r
ATP + guanidinoacetate
ADP + phosphoguanidinoacetate
Perinereis sp.
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-
-
?
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Ca2+
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slightly
Ca2+
Perinereis sp.
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29% of Mg2+-activation
Mg2+
Namalycastis sp.
essential for activation
Mg2+
Perinereis sp.
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5 mM, activation
Mn2+
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-
Mn2+
Perinereis sp.
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5 mM, activation
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3-guanidinopropionate
Perinereis sp.
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slight
4-Guanidinobutyrate
Perinereis sp.
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slight
5,5'-dithiobis(2-nitrobenzoate)
Perinereis sp.
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agmatine
Perinereis sp.
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Creatine
Perinereis sp.
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slight
guanidine
Perinereis sp.
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slight
iodoacetamide
Perinereis sp.
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-
L-arginine
Perinereis sp.
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slight
methylguanidine
Perinereis sp.
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slight
N-bromosuccinimide
Perinereis sp.
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phenylhydrazine
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inhibits phosphorylation of ADP
Taurocyamine
Perinereis sp.
-
slight
p-chloromercuribenzoate
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-
p-chloromercuribenzoate
Perinereis sp.
-
-
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0.33 - 4.1
Guanidinoacetate
6.8
phosphoguanidinoacetate
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-
0.8
ATP
Perinereis sp.
-
pH 8.1, 25°C
0.89
ATP
Namalycastis sp.
native GK
1.02
ATP
Namalycastis sp.
recombinant GK beta
3.38
glycocyamine
Namalycastis sp.
recombinant GK beta
4.7
glycocyamine
Namalycastis sp.
native GK
0.33
Guanidinoacetate
-
-
4.1
Guanidinoacetate
Perinereis sp.
-
pH 8.1, 25°C
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24
glycocyamine
Namalycastis sp.
recombinant GK beta
25.3
glycocyamine
Namalycastis sp.
native GK
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45.3
Perinereis sp.
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pH 8.1, 25°C
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6.8
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phosphorylation of ADP
8.1
Perinereis sp.
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phosphorylation of guanidinoacetate
8.9
-
phosphorylation of guanidinoacetate
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6.5 - 9
Perinereis sp.
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less than 50% of maximal activity above and below
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22
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phosphorylation of guanidinoacetate
35
Perinereis sp.
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phosphorylation of guanidinoacetate
40
-
optimum above, phosphorylation of ADP
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16 - 32
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less than 50% of maximal activity above and below, phosphorylation of guanidinoacetate
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brenda
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brenda
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brenda
three isoforms
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brenda
Perinereis sp.
polychaete
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-
brenda
Polycelis cornuta
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-
-
brenda
Namalycastis sp.
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SwissProt
brenda
Namalycastis sp.
alpha chain
SwissProt
brenda
Namalycastis sp.
beta chain
SwissProt
brenda
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Namalycastis sp.
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brenda
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-
brenda
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-
brenda
Polycelis cornuta
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-
brenda
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Namalycastis sp.
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brenda
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KGCY_HEDDI
393
0
44462
Swiss-Prot
other Location (Reliability: 2 )
Q6AW42_9ANNE
390
0
44038
TrEMBL
-
Q6AW43_9ANNE
374
0
42512
TrEMBL
-
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40000
Namalycastis sp.
SDS-PAGE
42200
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1 * 42200 + 1 * 43800, SDS-PAGE
43800
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1 * 42200 + 1 * 43800, SDS-PAGE
45000
Perinereis sp.
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1 * 47000 + 1 * 45000, SDS-PAGE
47000
Perinereis sp.
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1 * 47000 + 1 * 45000, SDS-PAGE
79000
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sucrose density gradient centrifugation
87500
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amino acid composition
89150
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equilibrium sedimentation centrifugation
90000
Perinereis sp.
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gel filtration
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heterodimer
Namalycastis sp.
heterodimer with two homologous polypeptide chains, alpha and beta, derived from a common pre mRNA by mutually exclusive N-terminal alternative exons
dimer
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1 * 42200 + 1 * 43800, SDS-PAGE
dimer
Namalycastis sp.
alphabeta, 2 * ca. 40000, SDS-PAGE
dimer
Perinereis sp.
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1 * 47000 + 1 * 45000, SDS-PAGE
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the crystal structures of recombinant glycocyamine kinase alphabeta and glycocyamine kinase betabeta from Namalycastis sp. are determined at 2.6 A and 2.4 A resolution, respectively. In addition, the structure of the glycocyamine kinase betabeta is determined at 2.3 A resolution in complex with a transition state analog, Mg2+ ADP-NO3- glycocyamine. In both heterodimeric and homodimeric glycocyamine kinase forms, the conformations of the two N-termini are asymmetric and the asymmetry is different than that reported previously for the homodimeric creatinine kinases from several organisms
Namalycastis sp.
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5.5 - 9.5
Perinereis sp.
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-
642364
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35
Perinereis sp.
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stable up to
40
Perinereis sp.
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20 min, 4% loss of activity
45
Perinereis sp.
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20 min, 28% loss of activity
50
Perinereis sp.
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inactivation
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the recombinant GK alpha enzyme is quite unstable, storage on ice for 4 h caused 10% loss of activity, in contrast GK beta enzyme is stable, the instability of GK alpha is most likely due to its expression as a maltose-binding protein fusion protein
Namalycastis sp.
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-20°C, 20 mM Tris-acetate buffer, pH 8.0, 1 mM dithiothreitol, 5% glycerol, several months
Perinereis sp.
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4°C, ammonium sulfate precipitate
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ammonium sulfate fractionation, gel filtration on Sephacryl S-200n, and DEAE-5PW ion exchange chromatography
Namalycastis sp.
using Ni-NTA chromatography
Namalycastis sp.
-
Perinereis sp.
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expressed in Escherichia coli as a His-tagged fusion protein
Namalycastis sp.
expression in Escherichia coli as maltose-binding protein fusion proteins
Namalycastis sp.
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Shirokane, Y.; Nakajima, M.; Mizusawa, K.
Purification and properties of guanidinoacetate kinase from a polychaete, Perineis sp.
Agric. Biol. Chem.
55
2235-2242
1991
Perinereis sp.
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brenda
Pradel, L.A.; Kassab, R.; Conlay, C.; van Thoai, N.
Properties and amino acid composition of purified ATP: guanidinoacetate phosphotransferase
Biochim. Biophys. Acta
154
305-314
1968
Nephtys caeca
brenda
Pradel, L.A.; Kassab, R.; van Thoai, N.
On ATP:guanidinoacetate N-phosphotransferase
Biochim. Biophys. Acta
81
86-95
1964
Nephtys caeca
-
brenda
Virden, R.; Watts, D.C.
Distribution of guanidine-adenosine triphosphate phosphotransferases and adenosintriphosphatase (ATPase) in animals from several phyla
Comp. Biochem. Physiol.
13
161-77
1964
Myxicola infundibulum, Polycelis cornuta
brenda
Furukohri, T.; Suzuki, T.
Preparation of glycocyamine kinase from polychaete, Perinereis brevicirris
Rep. USA Mar. Biol. Inst. Kochi Univ.
9
215ff
1987
Perinereis brevicirris
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brenda
Suzuki, T.; Nishimura, Y.; Umekawa, M.; Yamamoto, Y.; Kawamichi, H.; Furukohri, T.
Evolution of phosphagen kinase VII. Isolation of glycocyamine kinase from the polychaete Neanthes diversicolor and the cDNA-derived amino acid sequences of alpha and beta chains
J. Protein Chem.
18
13-19
1999
Hediste diversicolor
brenda
Mizuta, C.; Tanaka, K.; Suzuki, T.
Isolation, characterization, and cDNA-derived amino acid sequence of glycocyamine kinase from the tropical marine worm Namalycastis sp
Comp. Biochem. Physiol. B
140
387-393
2005
Namalycastis sp. (Q6AW42), Namalycastis sp. (Q6AW43)
brenda
Lim, K.; Pullalarevu, S.; Surabian, K.T.; Howard, A.; Suzuki, T.; Moult, J.; Herzberg, O.
Structural basis for the mechanism and substrate specificity of glycocyamine kinase, a phosphagen kinase family member
Biochemistry
49
2031-2041
2010
Namalycastis sp. (Q6AW42)
brenda
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