Information on EC 2.7.2.4 - aspartate kinase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
2.7.2.4
-
RECOMMENDED NAME
GeneOntology No.
aspartate kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + L-aspartate = ADP + 4-phospho-L-aspartate
show the reaction diagram
; The enzyme from E. coli is a multifunctional protein, which also catalyses the reaction of EC 1.1.1.3 homoserine dehydrogenase
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
Cysteine and methionine metabolism
-
ectoine biosynthesis
-
Glycine, serine and threonine metabolism
-
grixazone biosynthesis
-
homoserine biosynthesis
-
Lysine biosynthesis
-
lysine biosynthesis I
-
lysine biosynthesis II
-
lysine biosynthesis III
-
lysine biosynthesis VI
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
norspermidine biosynthesis
-
spermidine biosynthesis II
-
SYSTEMATIC NAME
IUBMB Comments
ATP:L-aspartate 4-phosphotransferase
The enzyme from Escherichia coli is a multifunctional protein, which also catalyses the reaction of EC 1.1.1.3 homoserine dehydrogenase. This is also the case for two of the four isoenzymes in Arabidopsis thaliana. The equilibrium constant strongly favours the reaction from right to left, i.e. the non-physiological direction of reaction.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AK
-
-
-
-
AK-R7
Saccharomyces cerevisiae HT1(pIVUts31d), Saccharomyces cerevisiae SG211
-
-
-
AK-ts31d
Saccharomyces cerevisiae HT1(pIVUts31d), Saccharomyces cerevisiae SG211
-
-
-
AK2
-, O23653
-
AKbeta
P74569
-
AKbeta
Synechocystis sp. PCC6803
P74569
-
-
AKbeta
P61489
-
AKsyn
P74569
-
AKsyn
Synechocystis sp. PCC6803
P74569
-
-
Ask
-, Q0KJ33
-
Ask
Streptomyces albulus CR1
-, Q0KJ33
-
-
ASK1
-, B0L9J2
-
ASK2
-, B0L9J2
-
aspartate kinase
-
-
aspartate kinase
Q9LYU8, Q9S702
-
aspartate kinase
-
-
aspartate kinase
P26512
-
aspartate kinase
Q9RUL9
-
aspartate kinase
Q9RUL9
-
-
aspartate kinase
P0A4Z8
-
aspartate kinase
-
-
aspartate kinase
P10869
-
aspartate kinase
Saccharomyces cerevisiae HT1(pIVUts31d), Saccharomyces cerevisiae SG211
-
-
-
aspartate kinase
-
-
aspartate kinase
P61489
-
aspartate kinase
-
-
aspartate kinase
-
-
aspartate kinase
B0L9J2
-
aspartate kinase (phosphorylating)
-
-
-
-
aspartate kinase 1
Q9LYU8
-
aspartate kinase 2
-
-
aspartate kinase 3
Q9S702
-
aspartate kinase beta
P74569
-
aspartate kinase beta
Synechocystis sp. PCC6803
P74569
-
-
aspartate kinase I
-
also acts as homoserine dehydrogenase (EC 1.1.1.3)
aspartate kinase II
-
also acts as homoserine dehydrogenase (EC 1.1.1.3)
aspartate kinase III
-
-
aspartic kinase
-
-
-
-
aspartokinase
-
-
-
-
aspartokinase
-
-
-
aspartokinase
-
-
aspartokinase
-
-
aspartokinase
-
-
-
aspartokinase
Q57991
-
aspartokinase
-
-
aspartokinase
-
-
aspartokinase
-
-
aspartokinase
-, Q0KJ33
-
aspartokinase
Streptomyces albulus CR1
-, Q0KJ33
-
-
aspartokinase 1-homoserine dehydrogenase 1
-
-
aspartokinase 1-homoserine dehydrogenase 1
Escherichia coli ATCC 9723
-
-
-
aspartokinase I
-
-
aspartokinase II
-
-
aspartokinase II
Bacillus subtilis VB217
-
-
-
aspartokinase III
-
-
aspartokinase-homoserine dehydrogenase
-
-
aspartokinase-homoserine dehydrogenase
-
-
beta-aspartokinase
-
-
-
-
dap-aspartokinase
-
mesodiaminopimelate-sensitive isoenzyme
HOM3 product
P10869
-
HOM3-R7 product
-
-
HOM3-R7 product
Saccharomyces cerevisiae HT1(pIVUts31d), Saccharomyces cerevisiae SG211
-
-
-
HOM3-ts31d product
-
-
HOM3-ts31d product
Saccharomyces cerevisiae HT1(pIVUts31d), Saccharomyces cerevisiae SG211
-
-
-
LT-aspartokinase
-
lysine-threonine-sensitive isoenzyme
lysC
-
gene name
MtbAKbeta
P0A4Z8
-
Thr-sensitive aspartate kinase
P61489
-
thrA
-
gene name
CAS REGISTRY NUMBER
COMMENTARY
9012-50-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Amycolatopsis lactamdurans LC411
LC411
-
-
Manually annotated by BRENDA team
; ligated into pET 23d(+) vector and Escherichia coli strain DH10B for cloning, expression in Escherichia coli BL21 (DE3) pLysS codon+
SwissProt
Manually annotated by BRENDA team
; Saccharomyces cerevisiae M20-20D cells are transformed with A. thaliana cDNA, protein expressed and purified from Saccharomyces cerevisiae, all data refer to the recombinant yeast protein
-
-
Manually annotated by BRENDA team
ligated into pET 23d(+) vector and Escherichia coli strain DH10B for cloning, expression in Escherichia coli BL21 (DE3) pLysS codon+
UniProt
Manually annotated by BRENDA team
var. Bensheim, mutant RL4
-
-
Manually annotated by BRENDA team
Azotobacter sp.
-
-
-
Manually annotated by BRENDA team
; chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus; chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus, and the beta subunit from Thermus flavus
-
-
Manually annotated by BRENDA team
VB217, ATCC6051
-
-
Manually annotated by BRENDA team
Bacillus subtilis VB217
VB217, ATCC6051
-
-
Manually annotated by BRENDA team
strain I110proC
-
-
Manually annotated by BRENDA team
Bradyrhizobium japonicum I110proC
strain I110proC
-
-
Manually annotated by BRENDA team
ATCC 10068
-
-
Manually annotated by BRENDA team
Corynebacterium flavescens N13
N13
-
-
Manually annotated by BRENDA team
ATCC 21799, nucleotide sequence encoding the beta-subunit
SwissProt
Manually annotated by BRENDA team
expression in Escherichia coli BL21-CodonPlus(DE3)-RIL cells with pET-26b(+) vector
SwissProt
Manually annotated by BRENDA team
carrot
-
-
Manually annotated by BRENDA team
strain R1, Deinococcus radiodurans AK has a different protein structure and a different evolutionary history from Thermus thermophilus AK, the disruption mutant exhibits a similar phenotype and the functions are not different
UniProt
Manually annotated by BRENDA team
strain R1, Deinococcus radiodurans AK has a different protein structure and a different evolutionary history from Thermus thermophilus AK, the disruption mutant exhibits a similar phenotype and the functions are not different
UniProt
Manually annotated by BRENDA team
9723 (ATCC)
-
-
Manually annotated by BRENDA team
strain B
-
-
Manually annotated by BRENDA team
strain K-12
-
-
Manually annotated by BRENDA team
Tir 8, derepressed strain of K 12 and mutant Gif 108
-
-
Manually annotated by BRENDA team
Escherichia coli ATCC 9723
9723 (ATCC)
-
-
Manually annotated by BRENDA team
Escherichia coli K12
K12
-
-
Manually annotated by BRENDA team
strain U112
-
-
Manually annotated by BRENDA team
barley
-
-
Manually annotated by BRENDA team
barley; L. cv. Jagriti
-
-
Manually annotated by BRENDA team
strain NCIMB 8826
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum NCIMB 8826
strain NCIMB 8826
-
-
Manually annotated by BRENDA team
strain MCS10
-
-
Manually annotated by BRENDA team
strain MCS10
-
-
Manually annotated by BRENDA team
Methanococcus jannaschii has only a single, monofunctional form of AK; pET-41a vector and Rosetta (DE3) Escherichia coli cells for protein expression
UniProt
Manually annotated by BRENDA team
strain AV19
-
-
Manually annotated by BRENDA team
homozygous tobacco plants expressing bacterialAK are crossed with homozygous transgenic tobacco lines expressing F-AtCGS, T-AtCGS (lacking its N-terminus region) or D-AtCGS (lacking 90-nt of the N-terminus region)
-
-
Manually annotated by BRENDA team
no activity in Edwardsiella sp.
-
-
-
Manually annotated by BRENDA team
no activity in Providencia sp.
-
-
-
Manually annotated by BRENDA team
no activity in Streptomyces akiyoshiensis
producer strain of 5-hydroxy-4-oxonorvaline
-
-
Manually annotated by BRENDA team
rice, IAC-165 upland variety
-
-
Manually annotated by BRENDA team
strain 63 (ATCC 25901)
-
-
Manually annotated by BRENDA team
Paenibacillus polymyxa 63
strain 63 (ATCC 25901)
-
-
Manually annotated by BRENDA team
wild-type strain P-14
-
-
Manually annotated by BRENDA team
strain HT1(pIVUts31d), has a feedback-resistent aspartate kinase; strain SG211, contains HOM3-R7 allel, insensitive to threonine
-
-
Manually annotated by BRENDA team
wildtype strain 8723c, parental strain of SG211
SwissProt
Manually annotated by BRENDA team
Saccharomyces cerevisiae HT1(pIVUts31d)
strain HT1(pIVUts31d), has a feedback-resistent aspartate kinase
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae SG211
strain SG211, contains HOM3-R7 allel, insensitive to threonine
-
-
Manually annotated by BRENDA team
Massa 03
-
-
Manually annotated by BRENDA team
strain BHT
-
-
Manually annotated by BRENDA team
Streptococcus mutans BHT
strain BHT
-
-
Manually annotated by BRENDA team
strain CR1
-
-
Manually annotated by BRENDA team
strain CR1, homologous expression in Streptomyces albulus CR1, expression with pQE30 plasmid in Escherichia coli M15 (pREP4)
TrEMBL
Manually annotated by BRENDA team
Streptomyces albulus CR1
strain CR1
-
-
Manually annotated by BRENDA team
Streptomyces albulus CR1
strain CR1, homologous expression in Streptomyces albulus CR1, expression with pQE30 plasmid in Escherichia coli M15 (pREP4)
TrEMBL
Manually annotated by BRENDA team
Synechocystis sp. PCC6803
-
UniProt
Manually annotated by BRENDA team
; chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus; chimera composed of the chimeric alpha subunit that comprises the N-terminal catalytic region from Bacillus subtilis AK II and the C-terminal region from Thermus flavus, and the beta subunit from Thermus flavus
-
-
Manually annotated by BRENDA team
; expression in Escherichia coli BL21-CodonPlus(DE3)-RIL cells
SwissProt
Manually annotated by BRENDA team
Thermus thermophilus AT-62
AT-62
-
-
Manually annotated by BRENDA team
transgenic narbon bean
-
-
Manually annotated by BRENDA team
Black Mexican Sweet; maize
-
-
Manually annotated by BRENDA team
Cat100-1 inbred line
-
-
Manually annotated by BRENDA team
expression in Saccharomyces cerevisiae sigma a3hu
TrEMBL
Manually annotated by BRENDA team
maize
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
A 2fold increase in lysine production is observed by cloning of the ASK gene in Corynebacterium glutamicum rather than in Escherichia coli, due to the presence of lysine exporter channel which facilitates lysine extraction
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
110
-
ATP
-
pH 8.0, 30C, mutant E257K
22040
170
-
ATP
-
pH 8.0, 30C, mutant E257K/T359I
22040
310
-
ATP
-
pH 8.0, 30C, wild-type
22040
370
-
ATP
-
pH 8.0, 30C, mutant T359I
22040
44
-
L-aspartate
-
pH 8.0, 30C, mutant E257K
12113
120
-
L-aspartate
-
pH 8.0, 30C, mutant E257K/T359I
12113
270
-
L-aspartate
-
pH 8.0, 30C, wild-type
12113
290
-
L-aspartate
-
pH 8.0, 30C, mutant T359I
12113
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.08
-
L-lysine
B0L9J2, -
Ask2-Oh545o2 and Ask2-Oh51Ao2; extract of transformed yeast hom3 cells by Ask2-Oh545o2 and Ask2-Oh51Ao2
1.4
-
L-lysine
-
pH 8.0, 30C, wild-type
121.5
-
L-lysine
-
pH 8.0, 30C, mutant E257K
145.4
-
L-lysine
-
pH 8.0, 30C, mutant T359I
157.2
-
L-lysine
-
pH 8.0, 30C, mutant E257K/T359I
0.3
-
L-threonine
-, Q57991
-
0.4
-
L-threonine
-
pH 8.0, 30C, mixture with L-lysine
0.7
-
L-threonine
-
pH 8.0, 30C, + 650 mM NaCl
2.9
-
L-threonine
-
pH 8.0, 30C
3.6
-
L-threonine
-
pH 8.0, 30C
3.9
-
L-threonine
-
pH 8.0, 30C, + 650 mM KCl
13.5
-
L-threonine
-
pH 8.0, 30C, + 650 mM KCl
18.7
-
L-threonine
-
pH 8.0, 30C, + 650 mM NaCl
PDB
SCOP
CATH
ORGANISM
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Neisseria meningitidis serogroup B (strain MC58)
Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Synechocystis sp. (strain PCC 6803 / Kazusa)
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
urea
-
enzyme is completely inactivated by 4 M
urea
Paenibacillus polymyxa 63
-
enzyme is completely inactivated by 4 M
-