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Information on EC 2.7.2.3 - phosphoglycerate kinase and Organism(s) Saccharolobus solfataricus and UniProt Accession P50317

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Saccharolobus solfataricus
UNIPROT: P50317 not found.
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The taxonomic range for the selected organisms is: Saccharolobus solfataricus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
pgk, phosphoglycerate kinase, pgk-1, 3-phosphoglycerate kinase, phosphoglycerate kinase 1, pgk-2, phosphoglycerate kinase-1, phosphoglycerokinase, phosphoglycerate kinase 2, 3-pgk, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-PGK
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3-phosphoglycerate kinase
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3-phosphoglycerate phosphokinase
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3-phosphoglyceric acid kinase
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3-phosphoglyceric acid phosphokinase
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3-phosphoglyceric kinase
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ATP-3-phospho-D-glycerate-1-phosphotransferase
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ATP:D-3-phosphoglycerate 1-phosphotransferase
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glycerate 3-phosphate kinase
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glycerophosphate kinase
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kinase (phosphorylating), phosphoglycerate
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phosphoglyceric acid kinase
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phosphoglyceric kinase
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phosphoglycerokinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
an ordered mechanism is assumed where 3-phospho-D-glycerate and 3-phospho-D-glyceroyl phosphate bind before ADP and ATP, respectively
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:3-phospho-D-glycerate 1-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9001-83-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
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-
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r
ADP + 3-phospho-D-glyceroyl phosphate
ATP + 3-phospho-D-glycerate
show the reaction diagram
-
the reaction occurs at 70°C
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r
ATP + 3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
the reaction occurs at 30°C
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-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + 3-phospho-D-glyceroyl phosphate
ATP + 3-phospho-D-glycerate
show the reaction diagram
-
the reaction occurs at 70°C
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-
r
ATP + 3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
the reaction occurs at 30°C
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r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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20 mM used in assay conditions
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.54
3-phospho-D-glycerate
70°C, pH 6.5
5.6
3-phospho-D-glyceroyl phosphate
70°C, pH 6.5
0.085
ADP
70°C, pH 6.5
9.7
ATP
70°C, pH 6.5
0.57
3-phospho-D-glycerate
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at pH 7.0 and 30°C
0.008
3-phospho-D-glyceroyl phosphate
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at pH 7.0 and 70°C
0.37
ADP
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at pH 7.0 and 70°C
9.31
ATP
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at pH 7.0 and 30°C
additional information
additional information
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.14
ADP
70°C, pH 6.5
1.02
ADP
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at pH 7.0 and 70°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
gluconeogenic pathway
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
x * 41000, SDS-PAGE
44395
x * 44395, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
heat treatment at 80°C is an effective first step in the purification of these recombinant enzyme from extracts of the Escherichia coli host
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli. The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from Sulfolobus solfataricus overlap by 8-bp
the gene encoding phosphoglycerate kinase is inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector is then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants are obtained on galactose plates at 37°C, but not at 28°C. These transformants contain low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enables complementation of the yeast pgk defect at 37°C but not at 28°C
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Piper, P.W.; Emson, C.; Jones, C.E.; Cowan, D.A.; Fleming, T.M.; Littlechild, J.A.
Complementation of a pgk deletion mutation in Saccharomyces cerevisiae with expression of the phosphoglycerate-kinase gene from the hyperthermophilic archaeon Sulfolobus solfataricus
Curr. Genet.
29
594-596
1996
Saccharolobus solfataricus
Manually annotated by BRENDA team
Jones, C.E.; Fleming, T.M.; Cowan, D.A.; Littlechild, J.A.; Piper, P.W.
The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli
Eur. J. Biochem.
233
800-808
1995
Saccharolobus solfataricus (P50317), Saccharolobus solfataricus
Manually annotated by BRENDA team
Kouril, T.; Esser, D.; Kort J.; Westerhoff, H.V.; Siebers, B.; Snoep, J.L.
Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus
FEBS J.
280
4666-4680
2013
Saccharolobus solfataricus (P50317), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (P50317)
Manually annotated by BRENDA team
Kouril, T.; Eicher, J.J.; Siebers, B.; Snoep, J.L.
Phosphoglycerate kinase acts as a futile cycle at high temperature
Microbiology
163
1604-1612
2017
Saccharomyces cerevisiae, Saccharolobus solfataricus
Manually annotated by BRENDA team