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Information on EC 2.7.2.3 - phosphoglycerate kinase and Organism(s) Mus musculus and UniProt Accession P09041

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Mus musculus
UNIPROT: P09041 not found.
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
pgk, phosphoglycerate kinase, pgk-1, 3-phosphoglycerate kinase, phosphoglycerate kinase 1, pgk-2, phosphoglycerate kinase-1, phosphoglycerokinase, phosphoglycerate kinase 2, 3-pgk, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoglycerate kinase 2
-
3-PGK
-
-
-
-
3-phosphoglycerate kinase
3-phosphoglycerate phosphokinase
-
-
-
-
3-phosphoglyceric acid kinase
-
-
-
-
3-phosphoglyceric acid phosphokinase
-
-
-
-
3-phosphoglyceric kinase
-
-
-
-
ATP-3-phospho-D-glycerate-1-phosphotransferase
-
-
-
-
ATP:D-3-phosphoglycerate 1-phosphotransferase
-
-
-
-
glycerate 3-phosphate kinase
-
-
-
-
glycerophosphate kinase
-
-
-
-
kinase (phosphorylating), phosphoglycerate
-
-
-
-
PGK
-
-
-
-
phosphoglycerate kinase 1
phosphoglycerate kinase 2
-
-
phosphoglyceric acid kinase
-
-
-
-
phosphoglyceric kinase
-
-
-
-
phosphoglycerokinase
-
-
-
-
X chromosome-linked phosphoglycerate kinase-1
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:3-phospho-D-glycerate 1-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-83-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + 3-phospho-D-glyceroyl phosphate
ATP + 3-phospho-D-glycerate
show the reaction diagram
-
-
-
r
3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl 1-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 3-phospho-D-glycerate
ADP + 1,3-diphosphoglycerate
show the reaction diagram
ATP + 3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
CTP + 3-phospho-D-glycerate
CDP + 1,3-diphosphoglycerate
show the reaction diagram
dATP + 3-phospho-D-glycerate
dADP + 1,3-diphosphoglycerate
show the reaction diagram
dGTP + 3-phospho-D-glycerate
dGDP + 1,3-diphosphoglycerate
show the reaction diagram
GTP + 3-phospho-D-glycerate
GDP + 1,3-diphosphoglycerate
show the reaction diagram
ITP + 3-phospho-D-glycerate
IDP + 1,3-diphosphoglycerate
show the reaction diagram
-
-
-
-
?
UTP + 3-phospho-D-glycerate
UDP + 1,3-diphosphoglycerate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + 3-phospho-D-glyceroyl phosphate
ATP + 3-phospho-D-glycerate
show the reaction diagram
-
-
-
r
ATP + 3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
divalent cation
-
Mg2+ or Mn2+
Mn2+
-
0.8 mM Mg2+ and 0.5 mM Mn2+ are optimal
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3-diphosphoglycerate
-
competitive to 3-phospho-D-glycerate and MgATP2-
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
ADP
-
inhibitory in forward reaction
citrate
-
40% inhibition at 50 mM
iodoacetate
-
-
N-ethylmaleimide
-
-
Urea
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no effect by glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-phosphate, pyruvate, phosphoenolpyruvate and lactate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.028 - 0.2
3-phospho-D-glycerate
0.039 - 0.23
ADP
0.038 - 0.42
ATP
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.3 - 3.8
2,3-diphosphoglycerate
0.08 - 0.175
ADP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
169
-
purified enzyme
260 - 450
-
purified isozyme PGKB
430
-
purified isozyme PGK2B
450
-
purified isozyme PGK2A
600
-
purified testis enzyme
710
-
purified enzyme
8.5
-
purified isozyme PGK2C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
-
-
6.5 - 8.5
-
-
7.6
-
assay at
8
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4 - 8
-
30% activity at pH 5.4, 60% activity at pH 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 40
-
activity of both isozymes in either reaction direction increases steadily with increasing temperature within this range
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
phosphoglycerate kinase 2 is a sperm-specific isoform
Manually annotated by BRENDA team
exclusively expressed in spermatids
Manually annotated by BRENDA team
-
from intraperitoneal cavity
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
there is no significant difference in cell proliferation between parental Lewis lung carcinoma (LLC-1) and LLC-1 transduced with PGK-1. Overexpression of PGK-1 in LLC-1 reduces the COX-2 expression, and, in turn, affects prostaglandin E2, cell invasion, angiogenesis, and the immune functions, and finally inhibit the tumor progression
Manually annotated by BRENDA team
-
2fold higher level than in testis
Manually annotated by BRENDA team
-
in early meiosis
Manually annotated by BRENDA team
-
isozyme PGK2
Manually annotated by BRENDA team
-
of the mouse lower first molar. Strong expression of Pgk1 mRNA in the odontogenic epithelial cells and surrounding mesenchymal cells of the tooth germ from embryonic day 10.5 to embryonic day 18.0
Manually annotated by BRENDA team
additional information
-
isozyme PGK1 is found in all somatic tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
targeted disruption of Pgk2 by homologous recombination eliminates PGK activity in sperm and testis, and severely impairs male fertility, but does not block spermatogenesis. Mating behavior, reproductive organ weights (testis, excurrent ducts, and seminal vesicles), testis histology, sperm counts, and sperm ultrastructure are indistinguishable between Pgk2(-/-) and wild-type mice. Sperm motility and ATP levels are markedly reduced in males lacking PGK2. Pgk2(-/-) males sire occasional pups. Alternative pathways that bypass the PGK step of glycolysis exist. One of these bypass enzymes, acylphosphatase, is active in mouse sperm, perhaps contributing to phenotypic differences between mice lacking GAPDHS or PGK2
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PGK2_MOUSE
417
0
44853
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
47000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 45000, SDS-PAGE
?
x * 48000, SDS-PAGE
monomer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallized in three forms: as the apoenzyme, as a complex with 3-phosphoglycerate, and as a complex with 3-phosphoglycerate and ATP. The crystal structures are solved to 2.7, 2.0, and 2.7 A resolutions, respectively
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7
-
isozyme PGKB: loss of 70% activity after 45 min, isozyme PGKA: loss of 10% activity after 45 min
642268
9.6
-
isozymes PGKA and PGKB: stable for at least 30 min
642268
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
pH 6.0, 10 min: 20% loss of activity, PGKA, 80% loss of activity PGKB
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin and dithioerythritol stabilize in the enzyme assay
-
optimal stabilization at 1 mM EDTA and 5 mM 2-mercaptoethanol
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
as a precipitate in 3.3 M ammonium sulfate, stable for years
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
876fold, to homogeneity
-
99fold from muscle, 333fold from testis
-
isoenzyme PGK1 and all 3 genetic variants of PGK2
-
isozymes PGKA and PGKB
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
129S6/SvEvTac genomic DNA fragments inserted into vector pUCBM21
-
1872-bp Pgk1/EGFP DNA fragment excised by double digestion of MluI and DraIII, consisting of 519 bp of the Pgk1 promoter, 723 bp of the EGFP cDNA and 224 bp of the bovine growth hormone (BGH) poly-A tail from the vector pPgk1/EGFP. Microinjection into the pronucleus of in vitro-fertilized FVB/NCrl mouse eggs and re-implanted into pseudopregnant ICR females to generate transgenic mice. CHO-K1 cells transfected with pPgk1/EGFP
3'UTR of PGK2 subcloned into the three regions F1, F2 and F3
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in the cytoplasm of early meiotic spermatocytes, t-KSRP promotes the decay of Pgk2 mRNA
-
Pgk2 mRNA is first detectable in early meiosis in spermatocytes and increases dramatically in spermatids. Decline of t-KSRP in late meiosis and increase of PTBP2 are concomitant with the increase in Pgk2 mRNA levels
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
in situ PCR is useful for detecting PGK-1 gene in paraffin-embedded sections under optimized conditions of both PCR cycle number and proteinase K concentration
medicine
additional information
-
polypyrimidine tract binding protein 2 is a trans-acting factor that helps to stabilize PGK2 mRNA in male mouse germ cells, specifically binds to the F1 region of the PGK2 3'-UTR
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kuntz, G.W.K.; Krietsch, W.K.G.
Phosphoglycerate kinase from animal tissue
Methods Enzymol.
90
103-110
1982
Oryctolagus cuniculus, Equus caballus, Homo sapiens, Mus musculus, trout
-
Manually annotated by BRENDA team
Lee, C.Y.
3-Phosphoglycerate kinase isozymes and genetic variants from mouse
Methods Enzymol.
90
121-126
1982
Mus musculus
-
Manually annotated by BRENDA team
Pegorado, B.; Lee, C.Y.
Purification and characterization of two isozymes of 3-phosphoglycerate kinase from the mouse
Biochim. Biophys. Acta
522
423-433
1978
Mus musculus, Mus musculus DBA/2J
Manually annotated by BRENDA team
Mukherjee, K.; Ghosh, S.; Ray, M.; Ray, S.
Purification and characterization of 3-phosphoglycerate kinase from Ehrlich ascites carcinoma cells
Indian J. Biochem. Biophys.
39
332-341
2002
Mus musculus
Manually annotated by BRENDA team
Xu, M.; Hecht, N.B.
Polypyrimidine tract binding protein 2 stabilizes phosphoglycerate kinase 2 mRNA in murine male germ cells by binding to its 3UTR
Biol. Reprod.
76
1025-1033
2007
Mus musculus
Manually annotated by BRENDA team
Honda, J.Y.; Kobayashi, I.; Kiyoshima, T.; Yamaza, H.; Xie, M.; Takahashi, K.; Enoki, N.; Nagata, K.; Nakashima, A.; Sakai, H.
Glycolytic enzyme Pgk1 is strongly expressed in the developing tooth germ of the mouse lower first molar
Histol. Histopathol.
23
423-432
2008
Mus musculus
Manually annotated by BRENDA team
Tang, S.J.; Ho, M.Y.; Cho, H.C.; Lin, Y.C.; Sun, G.H.; Chi, K.H.; Wang, Y.S.; Jhou, R.S.; Yang, W.; Sun, K.H.
Phosphoglycerate kinase 1-overexpressing lung cancer cells reduce cyclooxygenase 2 expression and promote anti-tumor immunity in vivo
Int. J. Cancer
123
2840-2848
2008
Mus musculus
Manually annotated by BRENDA team
Sawyer, G.M.; Monzingo, A.F.; Poteet, E.C.; OBrien, D.A.; Robertus, J.D.
X-ray analysis of phosphoglycerate kinase 2, a sperm-specific isoform from Mus musculus
Proteins
71
1134-1144
2008
Mus musculus (P09041), Mus musculus
Manually annotated by BRENDA team
Hishikawa, Y.; An, S.; Yamamoto-Fukuda, T.; Shibata, Y.; Koji, T.
Improvement of in situ PCR by optimization of PCR cycle number and proteinase k concentration: localization of x chromosome-linked phosphoglycerate kinase-1 gene in mouse reproductive organs
Acta Histochem. Cytochem.
42
15-21
2009
Mus musculus
Manually annotated by BRENDA team
Danshina, P.V.; Geyer, C.B.; Dai, Q.; Goulding, E.H.; Willis, W.D.; Kitto, G.B.; McCarrey, J.R.; Eddy, E.M.; OBrien, D.A.
Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice
Biol. Reprod.
82
136-145
2010
Mus musculus
Manually annotated by BRENDA team
Xu, M.; McCarrey, J.R.; Hecht, N.B.
A cytoplasmic variant of the KH-type splicing regulatory protein serves as a decay-promoting factor for phosphoglycerate kinase 2 mRNA in murine male germ cells
Nucleic Acids Res.
36
7157-7167
2008
Mus musculus
Manually annotated by BRENDA team
Wu, H.; Chou, C.; Hung, Y.; Yu, C.
Allotransplantation of transgenic mouse ovaries expressing enhanced green fluorescent protein under the control of the murine phosphoglycerate kinase 1 promoter
Reprod. Domest. Anim.
45
90090-6
2010
Mus musculus (P09411), Mus musculus
Manually annotated by BRENDA team
Mikawa, T.; Shibata, E.; Shimada, M.; Ito, K.; Ito, T.; Kanda, H.; Takubo, K.; Lleonart, M.E.; Inagaki, N.; Yokode, M.; Kondoh, H.
Phosphoglycerate mutase cooperates with Chk1 kinase to regulate glycolysis
iScience
23
101306
2020
Mus musculus (P09411)
Manually annotated by BRENDA team
Park, H.J.; Lee, W.Y.; Park, C.; Hong, K.H.; Kim, J.H.; Song, H.
Species-specific expression of phosphoglycerate kinase 2 (PGK2) in the developing porcine testis
Theriogenology
110
158-167
2018
Mus musculus (P09041), Mus musculus, Sus scrofa (Q6RI85), Sus scrofa
Manually annotated by BRENDA team