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Information on EC 2.7.2.2 - carbamate kinase and Organism(s) Trichomonas vaginalis and UniProt Accession O96432

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EC Tree
IUBMB Comments
The enzyme catalyses the reversible conversion of carbamoyl phosphate and ADP to ATP and carbamate, which hydrolyses to ammonia and hydrogencarbonate. The physiological role of the enzyme is to generate ATP.
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This record set is specific for:
Trichomonas vaginalis
UNIPROT: O96432
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The taxonomic range for the selected organisms is: Trichomonas vaginalis
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
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Synonyms
carbamate kinase, ckase, atp:carbamate phosphotransferase, carbamyl phosphokinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carbamoyl phosphokinase
-
-
-
-
carbamyl phosphokinase
-
-
-
-
CKase
-
-
-
-
kinase, carbamate (phosphorylating)
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:carbamate phosphotransferase
The enzyme catalyses the reversible conversion of carbamoyl phosphate and ADP to ATP and carbamate, which hydrolyses to ammonia and hydrogencarbonate. The physiological role of the enzyme is to generate ATP.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-69-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + carbamoyl phosphate
ATP + carbamate
show the reaction diagram
-
-
-
?
ATP + carbamate
ADP + carbamoyl phosphate
show the reaction diagram
-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + carbamate
ADP + carbamoyl phosphate
show the reaction diagram
-
-
-
r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2S,5S)-2-isopropyl-5-methylcyclohexanol
-
-
heptadecanoic acid
-
methyl (11Z)-11-icosen-14-ynoate
-
-
neophytadiene
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
Carbamoyl phosphate
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.47364
(1R,2S,5S)-2-isopropyl-5-methylcyclohexanol
pH and temperature not specified in the publication
-
0.72112
1-Dodecanol
pH and temperature not specified in the publication
1.71
elaidic acid
pH and temperature not specified in the publication
0.90996
heptadecanoic acid
pH and temperature not specified in the publication
0.33539
linoleic acid
pH and temperature not specified in the publication
0.48556
methyl (11Z)-11-icosen-14-ynoate
pH and temperature not specified in the publication
-
3.85
myristic acid
pH and temperature not specified in the publication
0.04047
neophytadiene
pH and temperature not specified in the publication
-
0.85567
palmitic acid
pH and temperature not specified in the publication
2.36
stearic acid
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1100
recombinant protein
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CBK_TRIVA
314
0
33927
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
x * 36000, SDS-PAGE, recombinant protein including His-tag
70000
gel filtration, recombinant protein
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 33920, calculated from amino acid sequence
dimer
x * 36000, SDS-PAGE, recombinant protein including His-tag
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Minotto, L.; Edwards, M.R.; Bagnara, A.S.
Trichomonas vaginalis: characterization, expression, and phylogenetic analysis of a carbamate kinase gene sequence
Exp. Parasitol.
95
54-62
2000
Trichomonas vaginalis (O96432), Trichomonas vaginalis
Manually annotated by BRENDA team
Shaikh, S.; Aaqil, H.; Rizvi, S.; Shakil, S.; Abuzenadah, A.; Gupta, P.; Saxena, S.; Tiwari, R.; Kumar, A.
Comparative inhibition study of compounds identified in the methanolic extract of Apamarga Kshara against Trichomonas vaginalis carbamate kinase (TvCK) an enzoinformatics approach
Interdiscip. Sci. Comput. Life Sci.
8
357-365
2016
Trichomonas vaginalis (O96432), Trichomonas vaginalis
Manually annotated by BRENDA team