Information on EC 2.7.2.18 - fatty acid kinase

-

-

?

ATP + acetate

ADP + acetyl phosphate

ATP + butyrate

ADP + butyryl phosphate

ATP + heptanoate

ADP + heptanoyl phosphate

22% activity compared to acetate

-

?

ATP + hexanoate

ADP + hexanoyl phosphate

38% activity compared to acetate

-

?

ATP + isobutyrate

ADP + isobutyryl phosphate

ATP + isovalerate

ADP + isovaleryl phosphate

-

-

?

ATP + myristic acid

ADP + myristyl phosphate

ATP + octanoate

ADP + octanoyl phosphate

8.4% activity compared to acetate

-

?

ATP + oleate

ADP + oleyl phosphate

ATP + oleic acid

ADP + oleyl phosphate

ATP + propionate

ADP + propionyl phosphate

ATP + valerate

ADP + valeryl phosphate

CTP + isobutyrate

CDP + isobutyryl phosphate

-

103% activity compared to ATP

-

?

GTP + isobutyrate

GDP + isobutyryl phosphate

-

70% activity compared to ATP

-

?

ITP + isobutyrate

IDP + isobutyryl phosphate

-

68% activity compared to ATP

-

?

UTP + isobutyrate

UDP + isobutyryl phosphate

-

78% activity compared to ATP

-

?

additional information

?

-

6 entries

ATP + oleic acid

ADP + oleyl phosphate

ATP

-

dependent on

Mg2+

Mn2+

Ca2+

-

complete inhibition at 4 mM Ca2+

Co2+

-

65% residual activity in the presence of 4 mM

Cu2+

-

58% residual activity in the presence of 4 mM

K+

-

complete inhibition at 4 mM K+

Na+

-

complete inhibition at 4 mM Na+

Zn2+

-

42% residual activity in the presence of 4 mM

10.8

2-methylbutyrate

-

at pH 7.5 and 30°C

53.9

acetate

at pH 5.7 and 37°C

1.8 - 3.1

ATP

16.9 - 24.5

Butyrate

4.3 - 109

Isobutyrate

9.5

Isovalerate

-

at pH 7.5 and 30°C

14.3 - 25.8

propionate

12.5 - 14.2

valerate

177

acetate

at pH 5.7 and 37°C

250

ATP

with acetate as cosubstrate, at pH 5.7 and 37°C

118

Butyrate

at pH 5.7 and 37°C

65.4

Isobutyrate

at pH 5.7 and 37°C

172

propionate

at pH 5.7 and 37°C

80.2

valerate

at pH 5.7 and 37°C

3.3

acetate

at pH 5.7 and 37°C

80.5

ATP

with acetate as cosubstrate, at pH 5.7 and 37°C

4.8

Butyrate

at pH 5.7 and 37°C

0.6

Isobutyrate

at pH 5.7 and 37°C

6.7

propionate

at pH 5.7 and 37°C

5.6

valerate

at pH 5.7 and 37°C

0.031

-

mutant enzyme T61A, at pH 7.5 and 37°C

0.034

-

mutant enzyme S93A, at pH 7.5 and 37°C

0.053

-

mutant enzyme H266A, at pH 7.5 and 37°C

0.497

-

mutant enzyme R202A, at pH 7.5 and 37°C

54.5

-

wild type enzyme, at pH 7.5 and 37°C

5.7

-

7.2

-

6 - 8.5

-

more than 80% activity between pH 6.0 and 8.5

51

activity declines sharply at temperatures above 51°C, with 44% and 12% of activity remaining at 60°C and 67°C, respectively

-

UniProt

Cereibacter sphaeroides ATH 2.4.1

-

UniProt

spirochete MA-2

-

Staphylococcus aureus RN4220

-

760788, 761247, 761390, 761398, 761457, 761798, 761929, 762267

-

-

761929

-

Staphylococcus aureus USA300

-

760788, 761247, 761398, 761798, 762267

-

malfunction

metabolism

physiological function

240000

-

FakA, gel filtration

762267

?

homodimer

subunit FakB2 bound to oleate, sitting drop vapor diffusion method, using 0.1 M citrate, pH 5.5, 20% (w/v) PEG 3000

-

H266A

-

the 1000fold less active mutant shows a reduced thermal denaturation temperature compared to the wild type enzyme

R170A

-

inactive

S93A

-

the 1000fold less active mutant shows a reduced thermal denaturation temperature compared to the wild type enzyme

T61A

-

the 1000fold less active mutant shows a reduced thermal denaturation temperature compared to the wild type enzyme

45 - 60

-

25% loss of activity after 30 min at 45°C, complete loss of activity after 30 min at 60°C

-50°C, crude cell extract, 2 months, no loss of activity

-

5°C, purified enzyme, 48 h, no loss of activity

-

DEAE-cellulose column chromatography and Sephacryl S-300 gel filtration

-

HisTrap column chromatography

Ni-NTA column chromatography

-

761798

Ni-NTA column chromatography and Superdex 75 gel filtration

-

expressed in Escherichia coli BL21 cells

-

761798

expressed in Escherichia coli BL21(DE3) cells

-

761457, 762267

expressed in Escherichia coli BL21(DE3) Rosetta-2 cells