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The taxonomic range for the selected organisms is: Synechocystis sp.
The enzyme appears in selected viruses and cellular organisms
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Rcp1 + ATP
?
Cph1 is a light-regulated histidine kinase that mediates red, far-red reversible phosphorylation of the a small response regulator Rcp1
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ATP + histidine kinase Hik34
ADP + histidine kinase Hik34 N-phospho-L-histidine
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
additional information
?
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the enzyme is involved in chemical sensing
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?
ATP + histidine kinase Hik34
ADP + histidine kinase Hik34 N-phospho-L-histidine
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histidine kinase Hik34 might negatively regulate the expression of certain heat shock genes that might by related to thermotolerance in Synechocystis
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?
ATP + histidine kinase Hik34
ADP + histidine kinase Hik34 N-phospho-L-histidine
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autophosphorylation, in vitro at physiological temperatures, but not at elevated temperatures, such as 44°C
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ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
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ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
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in Synechocystis sp. PCC 6803 four histidine kinases, Hik16, Hik33, Hik34, and Hik41, perceive and transduce salt signals
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?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
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in Synechocystis sp. PCC 6803 four histidine kinases, Hik16, Hik33, Hik34, and Hik41, perceive and transduce salt signals. The Hik16/Hik41 system responds only to NaCl
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ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
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the three-component system of histidine kinases and response regulator, His16-Hik41-Rre17, acts as transducer of hyperosmotic stress
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?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
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the two-component system of histidine kinase and response regulator, His10-Rre3, acts as transducer of hyperosmotic stress
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?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
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the two-component system of histidine kinase and response regulator, His33-Rre31, acts as transducer of hyperosmotic stress
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?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
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the two-component systes of histidine kinase and response regulator, His34-Rre1, acts as transducer of hyperosmotic stress
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?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Rcp1 + ATP
?
Cph1 is a light-regulated histidine kinase that mediates red, far-red reversible phosphorylation of the a small response regulator Rcp1
-
-
?
ATP + histidine kinase Hik34
ADP + histidine kinase Hik34 N-phospho-L-histidine
-
histidine kinase Hik34 might negatively regulate the expression of certain heat shock genes that might by related to thermotolerance in Synechocystis
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
additional information
?
-
the enzyme is involved in chemical sensing
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
-
in Synechocystis sp. PCC 6803 four histidine kinases, Hik16, Hik33, Hik34, and Hik41, perceive and transduce salt signals
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
-
in Synechocystis sp. PCC 6803 four histidine kinases, Hik16, Hik33, Hik34, and Hik41, perceive and transduce salt signals. The Hik16/Hik41 system responds only to NaCl
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
-
the three-component system of histidine kinases and response regulator, His16-Hik41-Rre17, acts as transducer of hyperosmotic stress
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
-
the two-component system of histidine kinase and response regulator, His10-Rre3, acts as transducer of hyperosmotic stress
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
-
the two-component system of histidine kinase and response regulator, His33-Rre31, acts as transducer of hyperosmotic stress
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
-
the two-component systes of histidine kinase and response regulator, His34-Rre1, acts as transducer of hyperosmotic stress
-
-
?
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malfunction
DeltaHik34 mutant cells are resistant to heat stress within its first hours, they cannot recover after 24 h long high temperature treatment, while the wild-type cell population is able to recover after 24 h of cultivation at 44°C. The damage caused by high temperature depends on many factors, which differ in these experiments: light intensity, CO2 content, the growth stage, or the growth temperature before heat stress. In DELTAHik34 mutant, the content of all pigments starts to decrease after 6 h of heat stress. Heat stress phenotypes, overview
malfunction
Hik2 mutants are not viable
malfunction
removal of the HAMP or the PAS domain from Hik33n-SphSc and substitutions of amino acid residues in the PAS domain influence kinase activity, overview. Subdomain-deleted variants of Hik33n-SphSc show reduced activity compared to the unaltered chimera
physiological function
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the histidine kinase SphS is involved in transcriptional activation of the phosphate (Pi)-acquisition system
physiological function
Hik2 is one of three essential histidine kinases in the cyanobacterium Synechocystis sp. PCC 6803. The signal input domain of Hik2 responds to environmental Cl- concentration
physiological function
histidine kinase Hik33 is one of the key regulators helping Synechocystis acclimate to multiple stress conditions, Hik33 may play important roles in allowing cells to acclimate to changing environmental conditions
physiological function
histidine kinase SphS senses phosphate-deficient conditions. SphS phosphorylates its cognate response regulator, SphR, under phosphate-deficient conditions and regulates the expression of the so-called pho regulon, which includes genes for acclimation to phosphate-deficient conditions, namely genes for a periplasmic alkaline phosphatase PhoA and phosphate transporters
physiological function
the enzyme Hik34 plays an important role in changes in transcriptome, proteome, lipidome, and photosynthesis in response to short term heat stress. Hik34 affects the expression of sets of genes under salt and osmotic stress, under normal conditions and heat stress and under oxidative stress
additional information
Hik2 contains a GAF (cGMP-specific phosphodiesterases, adenylyl cyclases and FhlA) domain in the signal input region
additional information
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Hik2 contains a GAF (cGMP-specific phosphodiesterases, adenylyl cyclases and FhlA) domain in the signal input region
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D156A
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity
D300A
site-directed mutagenesis, the mutation might change the dimeric configuration of the PAS domain, thereby enhancing the negative effect on kinase activity. The D300A-mutated PAS domain of Hik33 also forms dimer in the assay. The Hik33n-SphSc mutant shows reduced expression under non-stressed and salt-stressed conditions compared to unaltered Hik33n-SphSc
D389A
site-directed mutagenesis, the Hik33n-SphSc mutant shows only slightly reduced expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
D412N
site-directed mutagenesis, the Hik33n-SphSc mutant shows only slightly reduced expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
E416Q
site-directed mutagenesis, the Hik33n-SphSc mutant shows normal expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
G123A
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity
G405A
site-directed mutagenesis, the Hik33n-SphSc mutant shows only slightly reduced expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
G405S
site-directed mutagenesis, the Hik33n-SphSc mutant shows only slightly reduced expression under non-stressed conditions and no expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
N309A
site-directed mutagenesis, the Hik33n-SphSc mutant shows only slightly reduced expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
P114A
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity
Q141A
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity
Q22A
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2nHik7c construct is inactive in alkaline phosphatase activity
Q411E
site-directed mutagenesis, the Hik33n-SphSc mutant shows normal expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
R129K
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity
R377A
site-directed mutagenesis, the Hik33n-SphSc mutant shows only slightly reduced expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
R400S/R404A
site-directed mutagenesis, the Hik33n-SphSc mutant shows normal expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
R404A
site-directed mutagenesis, the Hik33n-SphSc mutant shows normal expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
R415E
site-directed mutagenesis, the mutation might change the dimeric configuration of the PAS domain, thereby enhancing the negative effect on kinase activity. The Hik33n-SphSc mutant shows reduced expression under non-stressed and salt-stressed conditions compared to unaltered Hik33n-SphSc
T409V
site-directed mutagenesis, the Hik33n-SphSc mutant shows only slightly reduced expression under non-stressed conditions and no expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
T414V
site-directed mutagenesis, the Hik33n-SphSc mutant shows only slightly reduced expression under non-stressed conditions and no expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
V395A
site-directed mutagenesis, the Hik33n-SphSc mutant shows only slightly reduced expression under non-stressed conditions and highly reduced expression under salt-stressed conditions compared to unaltered Hik33n-SphSc
W134F
site-directed mutagenesis, the point mutant of recombinant fused sensor, Hik2n-Hik7c construct is inactive in alkaline phosphatase activity
W318A
site-directed mutagenesis, the mutation might change the dimeric configuration of the PAS domain, thereby enhancing the negative effect on kinase activity. The W318A-mutated PAS domain of Hik33 also forms dimer in the assay. The Hik33n-SphSc mutant shows reduced expression under non-stressed and salt-stressed conditions compared to unaltered Hik33n-SphSc
additional information
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inactivation of each putative hik gene, no significant alterations in gene expression in most of the mutants. DeltaHik33 and deltaHik19 exhibit reduced ability to activate luciferase at low temperature. Deltahik27, deltahik34, and deltahik20 show enhanced expression of some genes, whereas others are repressed. In deltaHik34 cells, levels of transcripts of heat-shock genes are elevated. In deltaHik33-, deltaHik34-, deltaHik16-, and deltaHik41-mutant cells gene expression is significantly affected by elevated levels of NaCl
additional information
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mutation of hik41 does not affect the expression of the genes located downstream of this gene, 22 H2O2-inducible genes are either totally or almost totally unresponsive to H2O2 in deltaHik33 mutant cells, whereas mutation of Hik34, Hik16 or Hik41 abolishes the H2O2 induction of only two genes, Hik16 and Hik41 regulate the H2O2-inducible expression of the same two genes
additional information
construction of a fused sensor, Hik2n-Hik7c, which has the signal input domain of Hik2 and the kinase domain of the phosphate-deficiency sensor Hik7. The coding region of the hik7 gene is replaced with the fused sensor to evaluate the signalling activity in vivo as the activity of alkaline phosphatase (AP), which is regulated by Hik7. Cells expressing Hik2n-Hik7c have weak AP activities under standard growth conditions. Saline stress by NaCl induces AP activity in a dose-dependent manner. Hik2n-Hik7c responds to Cl- concentration. Amino acid substitutions in the signal input domain of Hik2 all result in loss of this responsiveness, i.e. mutants Q22A, P114A, G123A, R129K, W134F, Q141A and D156A completely lose AP activity
additional information
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construction of a fused sensor, Hik2n-Hik7c, which has the signal input domain of Hik2 and the kinase domain of the phosphate-deficiency sensor Hik7. The coding region of the hik7 gene is replaced with the fused sensor to evaluate the signalling activity in vivo as the activity of alkaline phosphatase (AP), which is regulated by Hik7. Cells expressing Hik2n-Hik7c have weak AP activities under standard growth conditions. Saline stress by NaCl induces AP activity in a dose-dependent manner. Hik2n-Hik7c responds to Cl- concentration. Amino acid substitutions in the signal input domain of Hik2 all result in loss of this responsiveness, i.e. mutants Q22A, P114A, G123A, R129K, W134F, Q141A and D156A completely lose AP activity
additional information
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generation of a series of chimeric proteins that include the N-terminal region of Hik33 (M1-A422) and the C-terminal region (Q197-P430) of SphS, a histidine kinase that senses phosphate-deficient conditions. The chimeric protein Hik33n-SphSc regulates expression of the phoA gene under stress conditions. Under standard growth conditions, cells that harbor the gene for Hik33n-SphSc express phoA, whereas the expression of phoA is repressed under salt stress and under cold stress
additional information
generation of a series of chimeric proteins that include the N-terminal region of Hik33 (M1-A422) and the C-terminal region (Q197-P430) of SphS, a histidine kinase that senses phosphate-deficient conditions. The chimeric protein Hik33n-SphSc regulates expression of the phoA gene under stress conditions. Under standard growth conditions, cells that harbor the gene for Hik33n-SphSc express phoA, whereas the expression of phoA is repressed under salt stress and under cold stress
additional information
-
generation of a series of chimeric proteins that include the N-terminal region of stress regulator histidine kinase Hik33 (M1-A422) and the C-terminal region (Q197-P430) of SphS. The chimeric protein Hik33n-SphSc regulates expression of the phoA gene under stress conditions. Under standard growth conditions, cells that harbor the gene for Hik33n-SphSc express phoA, whereas the expression of phoA is repressed under salt stress and under cold stress
additional information
generation of a series of chimeric proteins that include the N-terminal region of stress regulator histidine kinase Hik33 (M1-A422) and the C-terminal region (Q197-P430) of SphS. The chimeric protein Hik33n-SphSc regulates expression of the phoA gene under stress conditions. Under standard growth conditions, cells that harbor the gene for Hik33n-SphSc express phoA, whereas the expression of phoA is repressed under salt stress and under cold stress
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Bartsevich, V.V.; Shestakov, S.V.
The dspA gene product of the cyanobacterium Synechocystis sp. strain PCC 6803 influences sensitivity to chemically different growth inhibitors and has amino acid similarity to histidine protein kinases
Microbiology
141
2915-2920
1995
Synechocystis sp. (P20169)
-
brenda
Kaneko, T.; Sato, S.; Kotani, H.; et al.
Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions
DNA Res.
3
109-136
1996
Synechocystis sp. (P20169)
brenda
Reilly, P.; Hulmes, J.D.; Pan, Y.C.; Nelson, N.
Molecular cloning and sequencing of the psaD gene encoding subunit II of photosystem I from the cyanobacterium, Synechocystis sp. PCC 6803
J. Biol. Chem.
263
17658-17662
1988
Synechocystis sp. (P20169)
brenda
Kaneko, T.; Tanaka, A.; Sato, S.; Kotani, H.; Sazuka, T.; Miyajima, N.; Sugiura, M.; Tabata, S.
Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome
DNA Res.
2; 153-166
191-158
1995
Synechocystis sp. (Q55168)
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brenda
Park, C.M.; Shim, J.Y.; Yang, S.S.; Kang, J.G.; Kim, J.I.; Luka, Z.; Song, P.S.
Chromophore-apoprotein interactions in Synechocystis sp. PCC6803 phytochrome Cph1
Biochemistry
39
6349-6356
2000
Synechocystis sp. (Q55168)
brenda
Yeh, K.C.; Wu, S.H.; Murphy, J.T.; Lagarias, J.C.
A cyanobacterial phytochrome two-component light sensory system
Science
277
1505-1508
1997
Synechocystis sp. (Q55168)
brenda
Paithoonrangsarid, K.; Shoumskaya, M.A.; Kanesaki, Y.; Satoh, S.; Tabata, S.; Los, D.A.; Zinchenko, V.V.; Hayashi, H.; Tanticharoen, M.; Suzuki, I.; Murata, N.
Five histidine kinases perceive osmotic stress and regulate distinct sets of genes in Synechocystis
J. Biol. Chem.
279
53078-53086
2004
Synechocystis sp.
brenda
Suzuki, I.; Kanesaki, Y.; Hayashi, H.; Hall, J.J.; Simon, W.J.; Slabas, A.R.; Murata, N.
The histidine kinase Hik34 is involved in thermotolerance by regulating the expression of heat shock genes in Synechocystis
Plant Physiol.
138
1409-1421
2005
Synechocystis sp.
brenda
Marin, K.; Suzuki, I.; Yamaguchi, K.; Ribbeck, K.; Yamamoto, H.; Kanesaki, Y.; Hagemann, M.; Murata, N.
Identification of histidine kinases that act as sensors in the perception of salt stress in Synechocystis sp. PCC 6803
Proc. Natl. Acad. Sci. USA
100
9061-9066
2003
Synechocystis sp.
brenda
Murata, N.; Los, D.A.
Histidine kinase Hik33 is an important participant in cold-signal transduction in cyanobacteria
Physiol. Plant.
126
17-27
2006
Synechocystis sp.
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brenda
Kanesaki, Y.; Yamamoto, H.; Paithoonrangsarid, K.; Shoumskaya, M.; Suzuki, I.; Hayashi, H.; Murata, N.
Histidine kinases play important roles in the perception and signal transduction of hydrogen peroxide in the cyanobacterium, Synechocystis sp. PCC 6803
Plant J.
49
313-324
2007
Synechocystis sp.
brenda
Michel, K.P.; Schroeder, A.K.; Zimmermann, M.; Brandt, S.; Pistorius, E.K.; Frankenberg-Dinkel, N.; Staiger, D.
The hybrid histidine kinase Slr1759 of the cyanobacterium Synechocystis sp. PCC 6803 contains FAD at its PAS domain
Arch. Microbiol.
191
553-559
2009
Synechocystis sp.
brenda
Kimura, S.; Shiraiwa, Y.; Suzuki, I.
Function of the N-terminal region of the phosphate-sensing histidine kinase, SphS, in Synechocystis sp. PCC 6803
Microbiology
155
2256-2264
2009
Synechocystis sp.
brenda
Sakayori, T.; Shiraiwa, Y.; Suzuki, I.
A Synechocystis homolog of SipA protein, Ssl3451, enhances the activity of the histidine kinase Hik33
Plant Cell Physiol.
50
1439-1448
2009
Synechocystis sp.
brenda
Kotajima, T.; Shiraiwa, Y.; Suzuki, I.
Functional analysis of the N-terminal region of an essential histidine kinase, Hik2, in the cyanobacterium Synechocystis sp. PCC 6803
FEMS Microbiol. Lett.
351
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2014
Synechocystis sp. (P73276), Synechocystis sp.
brenda
Cerveny, J.; Sinetova, M.A.; Zavrel, T.; Los, D.A.
Mechanisms of high temperature resistance of Synechocystis sp. PCC 6803: an impact of histidine kinase 34
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Synechocystis sp. (P73276), Synechocystis sp.
brenda
Shimura, Y.; Shiraiwa, Y.; Suzuki, I.
Characterization of the subdomains in the N-terminal region of histidine kinase Hik33 in the cyanobacterium Synechocystis sp. PCC 6803
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53
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2012
Synechocystis sp., Synechocystis sp. (Q55586)
brenda